Cellular and environmental factors influencing biofilm formation and colonization of plant tissue by a beneficial strain of bacteria, Pseudomonas donghuensis P482.

2020 ◽  
Author(s):  
Magdalena Rajewska ◽  
Marta Matuszewska ◽  
Sylwia Jafra
Keyword(s):  
Antimicrobial Activity ◽  
Environmental Factors ◽  
Biofilm Formation ◽  
Genetic Background ◽  
Plant Pathogens ◽  
Knock Out ◽  
Factors Influencing ◽  
Fungal Plant Pathogens ◽  
Artificial Surfaces ◽  
Strain Type

<p>The ability to colonize different environmental niches by bacteria is most often determined by the ability to form biofilms - complex, multicellular communities. This, in turn, depends on both cellular and extracellular factors such as genetic background of the strain, type of surface (biotic or abiotic) to which bacteria attach, availability of nutrients, temperature, <em>etc</em>. <em>Pseudomonas donghuensis</em> P482 strain is a little-known isolate from tomato rhizosphere, exhibiting antimicrobial activity towards bacterial and fungal plant pathogens. Studies have shown that it efficiently colonizes plant rhizosphere and forms biofilm on artificial surfaces. Which genetic or environmental factors underlie the mechanism of biofilm formation were yet to be elucidated. The presented research aimed at identifying those factors. Basing on the analysis of genome, knock-out mutants of the P482 strain were constructed in the genes potentially involved in biofilm formation and further analyzed for motility, colony morphology, attachment to artificial surfaces in different culture conditions, and colonization of maize and tomato rhizosphere.</p>

scholarly journals The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marta Matuszewska ◽  
Tomasz Maciąg ◽  
Magdalena Rajewska ◽  
Aldona Wierzbicka ◽  
Sylwia Jafra
Keyword(s):  
Gene Expression ◽  
Antimicrobial Activity ◽  
Carbon Source ◽  
Reference Genes ◽  
Plant Pathogens ◽  
Plant Protection ◽  
Carbon Sources ◽  
Unknown Compound ◽  
Fungal Plant Pathogens ◽  
The Impact

AbstractPseudomonas donghuensis P482 is a tomato rhizosphere isolate with the ability to inhibit growth of bacterial and fungal plant pathogens. Herein, we analysed the impact of the carbon source on the antibacterial activity of P482 and expression of the selected genes of three genomic regions in the P482 genome. These regions are involved in the synthesis of pyoverdine, 7-hydroxytropolone (7-HT) and an unknown compound (“cluster 17”) and are responsible for the antimicrobial activity of P482. We showed that the P482 mutants, defective in these regions, show variations and contrasting patterns of growth inhibition of the target pathogen under given nutritional conditions (with glucose or glycerol as a carbon source). We also selected and validated the reference genes for gene expression studies in P. donghuensis P482. Amongst ten candidate genes, we found gyrB, rpoD and mrdA the most stably expressed. Using selected reference genes in RT-qPCR, we assessed the expression of the genes of interest under minimal medium conditions with glucose or glycerol as carbon sources. Glycerol was shown to negatively affect the expression of genes necessary for 7-HT synthesis. The significance of this finding in the light of the role of nutrient (carbon) availability in biological plant protection is discussed.

Production, partial purification, and antimicrobial activity of a novel antibiotic produced by a Bacillus subtilis isolate from Ulmus americana

1988 ◽  
Vol 66 (11) ◽  
pp. 2338-2346 ◽  
Author(s):  
L. R. Schreiber ◽  
Garold F. Gregory ◽  
C. R. Krause ◽  
J. M. Ichida
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
High Performance ◽  
Plant Pathogens ◽  
Antibiotic Activity ◽  
Dutch Elm Disease ◽  
Absolute Methanol ◽  
Partial Purification ◽  
Ulmus Americana ◽  
Fungal Plant Pathogens

Antibiotic substances produced by a Bacillus subtilis isolate from the xylem of an American elm (Ulmus americana L.) inhibited several fungal plant pathogens including the Dutch elm disease pathogen, Ceratocystis ulrni. Bioassays indicated the bacterium produced a diffusible, methanol-soluble antibiotic on potato dextrose agar at 30 °C. Cellulose powder column chromatography separated the antibiotic, which was further purified on a Sephadex LH-20 column, yielding two peaks of antibiotic activity, BS1 and BS2. These were further purified by paper chromatography. High-performance liquid chromatography, using C-18 reverse phase chromatography under isocratic conditions, resulted in five 280-nm absorbing peaks. Only one peak contained antibiotic activity. Upon ultrafiltration, the antibiotics passed through a 500 mol. wt. filter. The antibiotic was soluble in water, absolute methanol, ethanol, and chloroform but not in hexane or petroleum ether. Antimicrobial activity differed from that of other B. subtilis antibiotics including iturin A, bacillomycin L, mycosubtilin, fengycin, and bacilysin. Ceratocystis ulmi exposed to BS1 produced short, twisted, and swollen hyphae with irregularly thickened cell walls.

scholarly journals Necessity requires no decision: carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

2021 ◽  
Author(s):  
Marta Matuszewska ◽  
Tomasz Maciag ◽  
Magdalena Rajewska ◽  
Aldona Wierzbicka ◽  
Sylwia Jafra
Keyword(s):  
Gene Expression ◽  
Antimicrobial Activity ◽  
Carbon Source ◽  
Reference Genes ◽  
Plant Pathogens ◽  
Plant Protection ◽  
Carbon Sources ◽  
Unknown Compound ◽  
Fungal Plant Pathogens ◽  
The Impact

Abstract Pseudomonas donghuensis P482 is a tomato rhizosphere isolate with the ability to inhibit growth of bacterial and fungal plant pathogens. Herein, we analysed the impact of the carbon source on the antibacterial activity of P482 and expression of the selected genes of three genomic regions in the P482 genome. These regions are involved in the synthesis of pyoverdine, 7‑hydroxytropolone (7-HT) and an unknown compound (“cluster 17”) and are responsible for antimicrobial activity of P482. We showed that the P482 mutants, defective in these regions, show variations and contrasting patterns of growth inhibition of the target pathogen under given nutritional conditions (with glucose or glycerol as a carbon source). We also selected and validated the reference genes for gene expression studies in P. donghuensis P482. Amongst ten candidate genes, we found gyrB, rpoD and mrdA the most stably expressed. Using selected reference genes in RT-qPCR, we assessed the expression of the genes of interest under minimal medium conditions with glucose or glycerol as carbon sources. Glycerol was shown to negatively affect the expression of genes necessary for 7-HT synthesis. The significance of this finding in the light of the role of nutrient (carbon) availability in biological plant protection is discussed.

Peptidotriazoles with antimicrobial activity against bacterial and fungal plant pathogens

Peptides ◽  
2012 ◽  
Vol 33 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Imma Güell ◽  
Lluís Micaló ◽  
Laura Cano ◽  
Esther Badosa ◽  
Rafael Ferre ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Plant Pathogens ◽  
Fungal Plant Pathogens

Defensive Role of Plant-Derived Secondary Metabolites: Indole and Its’ Derivatives

2020 ◽  
Vol 9 (2) ◽  
pp. 78-88
Author(s):  
Mulugeta Mulat ◽  
Raksha Anand ◽  
Fazlurrahman Khan
Keyword(s):  
Biofilm Formation ◽  
Growth And Development ◽  
Functional Role ◽  
Plant Pathogens ◽  
Bacterial Species ◽  
Indole Derivatives ◽  
Resistance Level ◽  
Defensive Role ◽  
Insect Attack

The diversity of indole concerning its production and functional role has increased in both prokaryotic and eukaryotic systems. The bacterial species produce indole and use it as a signaling molecule at interspecies, intraspecies, and even at an interkingdom level for controlling the capability of drug resistance, level of virulence, and biofilm formation. Numerous indole derivatives have been found to play an important role in the different systems and are reported to occur in various bacteria, plants, human, and plant pathogens. Indole and its derivatives have been recognized for a defensive role against pests and insects in the plant kingdom. These indole derivatives are produced as a result of the breakdown of glucosinolate products at the time of insect attack or physical damages. Apart from the defensive role of these products, in plants, they also exhibit several other secondary responses that may contribute directly or indirectly to the growth and development. The present review summarized recent signs of progress on the functional properties of indole and its derivatives in different plant systems. The molecular mechanism involved in the defensive role played by indole as well as its’ derivative in the plants has also been explained. Furthermore, the perspectives of indole and its derivatives (natural or synthetic) in understanding the involvement of these compounds in diverse plants have also been discussed.

Morphological, biochemical and molecular identification of rhizobacteria isolates with potential for biocontrol of fungal plant pathogens

2021 ◽  
pp. 1-14
Author(s):  
Stephen Larbi-Koranteng ◽  
Richard Tuyee Awuah ◽  
Fredrick Kankam ◽  
Muntala Abdulai ◽  
Marian Dorcas Quain ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Plant Pathogens ◽  
Fungal Plant Pathogens
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