Influence of pore geometry on motility and trapping of metal reducing bacteria
<p>Diverse processes such as bioremediation, biofertilization, and microbial drug delivery<br>rely on bacterial migration in porous media. However, how pore-scale confinement alters<br>bacterial motility is unknown due to the inherent heterogeneity in porous media. As a<br>result, models of migration are limited and often employ ad hoc assumptions.<br>We aim to determine the impact of pore confinement in the spreading dynamics of two<br>populations of motile metal reducing bacteria by directly visualizing individual <em>Acidovorax</em><br>and <em>Pelosinus</em> in an unconfined liquid medium and in a microfluidic chip containing regular<br>placed pillars. We observe that the length of runs of the two species differs from the<br>unconfined and confined medium. Results show that bacteria in the confined medium<br>display a systematic shorter jumps due to grain obstacles when compared to the open<br>porous medium. Close inspection of the trajectories reveals that cells are intermittently<br>and transiently trapped, which produces superdiffusive motion at early and subdiffusion<br>behavior at late times, as they navigate through the confined pore space. While in the open<br>medium, we observe a linearly increasing variance with respect to time for <em>Acidovorax</em>, and<br>for <em>Pelosinus</em> the variance increases at a much faster rate showing super diffusive behavior<br>at early times. At late times, the rate of growth in spreading increases for <em>Acidovorax</em> while<br>it reduces for <em>Pelosinus</em>. We finally discuss that the paradigm of run-and-tumble motility<br>is dramatically altered in the confined porous medium and its practical applications of<br>these effects on large-scale transport.</p>