scholarly journals Occurrence of Escherichia coli in cloacal samples of broiler chicken from Kollam and Kottayam districts

2021 ◽  
Vol 52 (4) ◽  
Author(s):  
Afsal S. ◽  
Latha C. ◽  
Sethulekshmi C. ◽  
Binsy M. ◽  
Beena C. J. ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Broiler Chicken ◽  
One Health ◽  
Holistic Approach ◽  
Cloacal Swab ◽  
Zoonotic Diseases ◽  
Foodborne Illness ◽  
Culture Techniques ◽  
E Coli ◽  
Conventional Culture

Foodborne pathogens like E. coli are considered as the major causes of foodborne illness in humans worldwide. The present study was undertaken to determine the occurrence of E. coli in cloacal samples of broiler chicken from Kollam and Kottayam districts. The occurrence of E. coli in cloacal samples from broiler chicken was 76.5 per cent from Kollam and 79 per cent from Kottayam through culture techniques. Out of the total 400 cloacal swab samples collected from broiler chicken, 77.8 per cent were positive for E. coli. The samples which were subjected to conventional culture techniques were further analysed for PCR confirmation. The study revealed that, 56.5 and 67 per cent samples were positive for E. coli from Kollam and Kottayam, respectively. An overall occurrence of 61.8 per cent out of 400 samples were confirmed for E. coli by PCR. One Health approach can be used as a suitable tool to combat the foodborne zoonotic diseases, since it is an integrated, multidisciplinary, holistic approach. Proper implementation of biosecurity measures in farms is mandatory to control foodborne zoonotic diseases.

scholarly journals Antimicrobial Resistance of Escherichia coli and Salmonella isolated from Raw Retail Broiler Chicken Carcasses in Zambia

2020 ◽  
Author(s):  
Elizabeth Muligisa Muonga ◽  
Geoffrey Mainda ◽  
Mercy Mukuma ◽  
Geoffrey Kwenda ◽  
Bernard Hang'ombe ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Foodborne Pathogens ◽  
Broiler Chicken ◽  
Target Genes ◽  
Health Concern ◽  
Public Health Concern ◽  
E Coli ◽  
Genes Encoding

Abstract BackgroundAntimicrobial resistance (AMR) of foodborne pathogens is of public health concern, especially in developing countries such as Zambia. This study was undertaken to determine the antimicrobial resistance profiles of Escherichia coli ( E. coli ) and Salmonella isolated from raw retail broiler chicken carcasses purchased from open and supermarkets in Zambia.ResultsA total of 189 E. coli and five Salmonella isolates were isolated. Identification and confirmation of the isolates were done using Analytical Profile Index (API 20E) (Biomerieux ® ) and 16S rRNA sequencing. Antimicrobial susceptibility tests (AST) were performed using the Kirby Bauer disk diffusion technique using a panel of 10 antibiotics. Multiplex PCR was used to determine the presence of three target genes encoding for resistance: tet A, Sul 1 and bla CTX-M . WHONET 2018 software was used to analyse AST results. The E. coli isolates were mostly resistant to tetracycline (79.4%), ampicillin (51.9%), and trimethoprim/sulfamethoxazole (49.7%). Two of the five Salmonella isolates were resistant to at least one antibiotic. Forty- seven (45.2%) of the 104 isolates that were screened for the presence of the resistant genes possessed at least one of the targeted resistance genes.ConclusionThis study has demonstrated the presence of AMR E. coli and Salmonella on raw retail broiler chicken carcasses from open and supermarkets, which is of public health concern.

Evaluation of Detection Methods for Screening Meat and Poultry Products for the Presence of Foodborne Pathogens

2005 ◽  
Vol 68 (12) ◽  
pp. 2637-2647 ◽  
Author(s):  
VALERIE M. BOHAYCHUK ◽  
GARY E. GENSLER ◽  
ROBIN K. KING ◽  
JOHN T. WU ◽  
LYNN M. McMULLEN
Keyword(s):  
Foodborne Pathogens ◽  
Culture Method ◽  
Lateral Flow ◽  
Detection Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Meat ◽  
Culture Methods ◽  
E Coli ◽  
Poultry Products ◽  
Conventional Culture

Rapid and molecular technologies such as enzyme-linked immunosorbent assay (ELISA), PCR, and lateral flow immunoprecipitation can reduce the time and labor involved in screening food products for the presence of pathogens. These technologies were compared with conventional culture methodology for the detection of Salmonella, Campylobacter, Listeria, and Escherichia coli O157:H7 inoculated in raw and processed meat and poultry products. Recommended protocols were modified so that the same enrichment broths used in the culture methods were also used in the ELISA, PCR, and lateral flow immunoprecipitation assays. The percent agreement between the rapid technologies and culture methods ranged from 80 to 100% depending on the pathogen detected and the method used. ELISA, PCR, and lateral flow immunoprecipitation all performed well, with no statistical difference, compared with the culture method for the detection of E. coli O157:H7. ELISA performed better for the detection of Salmonella, with sensitivity and specificity rates of 100%. PCR performed better for the detection of Campylobacter jejuni, with 100% agreement to the culture method. PCR was highly sensitive for the detection of all the foodborne pathogens tested except Listeria monocytogenes. Although the lateral flow immunoprecipitation tests were statistically different from the culture methods for Salmonella and Listeria because of false-positive results, the tests did not produce any false negatives, indicating that this method would be suitable for screening meat and poultry products for these pathogens.

Antimicrobial action of rabbit leukocyte CAP18(106-137).

1997 ◽  
Vol 41 (3) ◽  
pp. 624-629 ◽  
Author(s):  
D J Mason ◽  
R Dybowski ◽  
J W Larrick ◽  
V A Gant
Keyword(s):  
Membrane Potential ◽  
Colony Formation ◽  
Flow Cytometric Analysis ◽  
Membrane Integrity ◽  
Broth Culture ◽  
Antimicrobial Protein ◽  
Culture Techniques ◽  
E Coli ◽  
Conventional Culture ◽  
Solid Media

CAP18 is a cationic antimicrobial protein originally isolated from rabbit neutrophils, of which a 32-mer sequence from its C-terminal and (CAP18(106-137)) has been found to be the most active. The bactericidal action of this peptide has been characterized by conventional culture techniques and flow cytometry. Cultures of Escherichia coli NCTC10418 were exposed to the MBC (12 microM) of the peptide for up to 60 min and stained with a fluorochrome sensitive to changes in either membrane potential (bis-(1,3-dibutylbarbituric acid)trimethine oxonol [DiBAC4(3)), or membrane integrity (propidium iodide [PI]) before flow cytometric analysis. Addition of CAP18(106-137) to E. coli in broth culture resulted in immediate collapse of membrane potential [as determined by uptake of DiBAC4(3)] and loss of membrane integrity (as indicated by uptake of PI), with a corresponding 6- to 8-log decrease in viable counts as determined by colony formation on solid media. In identical experiments, the presence of Mg2+ (1 to 10 mM), K+ (50 to 250 mM), or EDTA (5 mM) or incubation in nutrient-free buffer or at 4 degrees C had no effect on peptide-induced dye uptake. In contrast, addition of Ca2+ (1 to 10 mM) or the respiratory chain poison carbonyl cyanide m-chlorophenylhydrazone (CCCP) (50 microM) inhibited the uptake of both dyes. These findings, however, did not relate to bacterial recovery on solid media, where (unless in the presence of K+ 150 to 250 mM) CAP18(106-137) at 12 microM fulfilled the MBC criteria (99.9% killing). We conclude that CAP18(106-137) exerts a rapid and profound action on E. coli cytoplasmic membranes and viability as measured by colony formation. The results suggest, however, that CAP18(106-137) may exert its action at sites additional to the cell membrane and that its activity profile is unique among cationic antimicrobial proteins.

scholarly journals Preventing Foodborne Illness: E. coli “The Big Six”

EDIS ◽  
2018 ◽  
Vol 2018 (1) ◽  
Author(s):  
Bruna Bertoldi ◽  
Susanna Richardson ◽  
Renee Goodrich Schneider ◽  
Ploy Kurdmongkolthan ◽  
Keith R. Schneider
Keyword(s):  
Foodborne Pathogens ◽  
Foodborne Illness ◽  
Human Nutrition ◽  
Food Science ◽  
Food Handlers ◽  
E Coli ◽  
Fact Sheet ◽  
Big Six

This 7-page fact sheet is one in a series of fact sheets discussing common foodborne pathogens of interest to food handlers, processors, and retailers. It covers the characteristics of, and symptoms caused by, the bacterium E. coli (particularly the “big six” strains), and also details how to minimize the risk of spreading or contracting an E. coli infection. Written by Bruna Bertoldi, Susanna Richardson, Renee Goodrich-Schneider, Ploy Kurdmongkoltham, and Keith R. Schneider and published by the UF/IFAS Department of Food Science and Human Nutrition, January 2018. http://edis.ifas.ufl.edu/fs233

scholarly journals The impact of socioeconomic status on foodborne illness in high-income countries: a systematic review

2015 ◽  
Vol 143 (12) ◽  
pp. 2473-2485 ◽  
Author(s):  
K. L. NEWMAN ◽  
J. S. LEON ◽  
P. A. REBOLLEDO ◽  
E. SCALLAN
Keyword(s):  
Systematic Review ◽  
Socioeconomic Status ◽  
Low Socioeconomic Status ◽  
Foodborne Pathogens ◽  
Developed Countries ◽  
Foodborne Illness ◽  
Low Socioeconomic ◽  
E Coli ◽  
Developed Nations ◽  
The Impact

SUMMARYFoodborne illness is a major cause of morbidity and loss of productivity in developed nations. Although low socioeconomic status (SES) is generally associated with negative health outcomes, its impact on foodborne illness is poorly understood. We conducted a systematic review to examine the association between SES and laboratory-confirmed illness caused by eight important foodborne pathogens. We completed this systematic review using PubMed for all papers published between 1 January 1980 and 1 January 2013 that measured the association between foodborne illness and SES in highly developed countries and identified 16 studies covering four pathogens. The effect of SES varied across pathogens: the majority of identified studies for Campylobacter, salmonellosis, and E. coli infection showed an association between high SES and illness. The single study of listeriosis showed illness was associated with low SES. A reporting bias by SES could not be excluded. SES should be considered when targeting consumer-level public health interventions for foodborne pathogens.

scholarly journals Preventing Foodborne Illness: E. coli O157:H7

EDIS ◽  
1969 ◽  
Vol 2003 (3) ◽  
Author(s):  
Keith R. Schneider ◽  
Renée M. Goodrich ◽  
Melissa A. Kirby
Keyword(s):  
Foodborne Pathogens ◽  
Cooperative Extension ◽  
Cooperative Extension Service ◽  
Foodborne Illness ◽  
Human Nutrition ◽  
Extension Service ◽  
Food Science ◽  
Food Handlers ◽  
University Of Florida ◽  
E Coli

This is one in a series of fact sheets discussing common foodborne pathogens of interest to food handlers, processors, and retailers.  For related publications, visit http://edis.ifas.ufl.edu/topic_foodborne_illness. This document is FSHN031, one of a series of the Food Science and Human Nutrition Department, Florida Cooperative Extension Service, IFAS, University of Florida. Publication: January 2003. FSHN031/FS097: Preventing Foodborne Illness: E. coli O157:H7 (ufl.edu)

scholarly journals Antimicrobial Resistance of Escherichia coli and Salmonella isolated from Raw Retail Broiler Chicken Carcasses in Zambia

2020 ◽  
Author(s):  
Elizabeth Muligisa Muonga ◽  
Geoffrey Mainda ◽  
Mercy Mukuma ◽  
Geoffrey Kwenda ◽  
Bernard Hang'ombe ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Foodborne Pathogens ◽  
Broiler Chicken ◽  
Target Genes ◽  
Health Concern ◽  
Public Health Concern ◽  
E Coli ◽  
Genes Encoding

Abstract Background Antimicrobial resistance (AMR) of foodborne pathogens is of public health concern, especially in developing countries such as Zambia. This study was undertaken to determine the antimicrobial resistance profiles of Escherichia coli (E. coli) and Salmonella isolated from raw retail broiler chicken carcasses purchased from open and supermarkets in Zambia.Results A total of 189 E. coli and five Salmonella isolates were isolated. Identification and confirmation of the isolates were done using Analytical Profile Index (API 20E) (Biomerieux®) and 16S rRNA sequencing. Antimicrobial susceptibility tests (AST) were performed using the Kirby Bauer disk diffusion technique using a panel of 10 antibiotics. Multiplex PCR was used to determine the presence of three target genes encoding for resistance: tetA, Sul1 and blaCTX-M. WHONET 2018 software was used to analyse AST results. The E. coli isolates were mostly resistant to tetracycline (79.4%), ampicillin (51.9%), and trimethoprim/sulfamethoxazole (49.7%). Two of the five Salmonella isolates were resistant to at least one antibiotic. Forty- seven (45.2%) of the 104 isolates that were screened for the presence of the resistant genes possessed at least one of the targeted resistance genes.Conclusion This study has demonstrated the presence of AMR E. coli and Salmonella on raw retail broiler chicken carcasses from open and supermarkets, which is of public health concern.

scholarly journals Bacteriological assessments of foodborne pathogens in poultry meat at different super shops in Dhaka, Bangladesh

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Jalal Uddin ◽  
Khaled Hossain ◽  
Saddam Hossain ◽  
Karabi Saha ◽  
Fatema Tuz Jubyda ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Culture Method ◽  
Poultry Meat ◽  
Salmonella Spp ◽  
E Coli ◽  
Poultry Products ◽  
Enteropathogenic Bacteria ◽  
Conventional Culture ◽  
Shigella Spp ◽  
Meat Samples

Poultry is now considered as a major fast-growing source of meat in the world. The consumers demand safe and hygienic products without contamination with pathogenic microorganisms when the production and consumption of poultry meat is gradually increasing. The present study was conducted to assess the bacterial contamination of dressed chicken collected from different supershops in Dhaka, Bangladesh. The chicken samples from S1, S2, M1, M2 and A supershops were analyzed to determine the enteropathogenic bacteria in poultry meat. Three genera of bacteria were isolated from all of the chicken meat samples. These enteropathogens from various organs of dressing chickens were also enumerated. The isolates were presumptively identified as E. coli, Salmonella spp., and Shigella spp. by conventional culture method. The three enteropathogens were subjected to PCR assay for their confirmation as virulent enteropathogens. Only E. coli isolates were confirmed as pathogenic E. coli (Enterotoxigenic), other isolates were not confirmed as virulent Salmonella spp., Shigella spp.. Results of this study demonstrated that more cautions are recommended for personnel hygiene in processing and handling of poultry and poultry products to prevent occurrence of enterotoxigenic E. coli in dressed poultry meat sold by the supershops in Bangladesh.

scholarly journals Antimicrobial resistance of Escherichiacoli and Salmonella isolated from retail broiler chicken carcasses in Zambia

2021 ◽  
Vol 6 (1) ◽  
pp. 35
Author(s):  
Elizabeth Muligisa-Muonga ◽  
Geoffrey Mainda ◽  
Mercy Mukuma ◽  
Geoffrey Kwenda ◽  
Bernard Hang’ombe ◽  
...  
Keyword(s):  
Public Health ◽  
Antimicrobial Resistance ◽  
Foodborne Pathogens ◽  
Broiler Chicken ◽  
Health Concern ◽  
Public Health Concern ◽  
E Coli ◽  
Antimicrobial Susceptibility Tests ◽  
Susceptibility Tests ◽  
Open Markets

Antimicrobial resistance (AMR) of foodborne pathogens is of public health concern, especially in developing countries such as Zambia. This study was undertaken to determine the antimicrobial resistance profiles of Escherichia coli (E. coli) and Salmonella isolated from retail broiler chicken carcasses purchased from open markets and supermarkets in Zambia. A total of 189 E. coli and five Salmonella isolates were isolated. Identification and confirmation of the isolates were done using Analytical Profile Index (API 20E) (Biomerieux®) and 16S rRNA sequencing. Antimicrobial susceptibility tests (AST) were performed using the Kirby Bauer disk diffusion technique using a panel of 9 antibiotics. WHONET 2018 software was used to analyze AST results. The E. coli isolates were mostly resistant to tetracycline (79.4%), ampicillin (51.9%), and trimethoprim/sulfamethoxazole (49.7%). Two of the five Salmonella isolates were resistant to at least one antibiotic. This study has demonstrated the presence of AMR E. coli and Salmonella on retail broiler chicken carcasses from open markets and supermarkets, which is of public health concern.

Comparisons of Non-thermal Decontamination Methods to Improve the Safety for Raw Beef Consumption

2021 ◽  
Author(s):  
Sangeun Park ◽  
Eunyoung Park ◽  
Yohan Yoon
Keyword(s):  
Foodborne Pathogens ◽  
Hypochlorous Acid ◽  
High Pressure Processing ◽  
Foodborne Illness ◽  
Thermal Treatments ◽  
Quality Properties ◽  
E Coli ◽  
Cell Counts ◽  
Uv Led ◽  
Thermal Decontamination

The object of this study was to examine non-thermal treatments to reduce foodborne pathogens in raw beef. Foodborne-illness pathogens were inoculated in the raw beef. Death rates of foodborne illness pathogens were evaluated by non-thermal decontamination methods(high pressure processing at 500MPa[HPP] for 2min, 5min, and 7min; UV LED radiation at 405nm[UV LED] for 2h, 6h, and 24h; hypochlorous acid water at 100ppm[HAW] for 1min, 3min, and 5min; 2.5% lactic acid[LA] for 1min, 3min, and 5min; modified atmosphere that replaced O2 to CO2 [MAP] for 24h and 48​​h; bio-gel[BG] application for 24h and 48h. Quality characteristics were measured after applying the practical non-thermal decontamination methods. After the treatment of HPP for 7min, inactivity rates were 4.4-6.7Log CFU/g for E. coli, Salmonella, and L. monocytogenes and 1.7Log CFU/g for S. aureus (p <0.05). After the treatment with UV LED for 24h, the reduced cell counts were 0.5, 0.7, and 0.3Log CFU/g for E. coli , Salmonella , and S. aureus, respectively(p <0.05), but no significant reduction for L. monocytogenes. When the beef was treated with HAW was treated for 5min, 0.6Log CFU/g of E. coli, 0.5Log CFU/g of Salmonella, 0.4Log CFU/g of S. aureus , and 0.5Log CFU/g of L. monocytogenes were inactivated. After the beef was treated with LA for 5min, 1.8Log CFU/g of E. coli, 3.0Log CFU/g of Salmonella, 1.3Log CFU/g of S. aureus, and 1.9Log CFU/g of L. monocytogenes were inactivated. MAP for 48h caused the inactivation of 0.3Log CFU/g of E. coli, 0.1Log CFU/g of Salmonella. After treatment of BG for 48h, 0.3Log CFU/g of E. coli and 0.4Log CFU/g of Salmonella were significantly decreased(p <0.05). HPP cooked the beef after 2min of treatment. HAW and BG changed the surface color of the beef, LA reduced the pH of beef (p<0.05). However, UV LED did not cause any changes in the beef quality properties. These results indicates that UV LED can improve the food safety of raw beef.

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