BUILDING EXTENSIBLE 3D INTERACTION METADATA WITH INTERACTION INTERFACE CONCEPT

2009 ◽  
Keyword(s):  
3D Interaction ◽  
Interaction Interface

scholarly journals Erythrocytes 3D genome organization in vertebrates

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Anastasia Ryzhkova ◽  
Alena Taskina ◽  
Anna Khabarova ◽  
Veniamin Fishman ◽  
Nariman Battulin
Keyword(s):  
Blood Cells ◽  
Genome Organization ◽  
Large Scale ◽  
Chromatin Condensation ◽  
Erythroid Differentiation ◽  
Interaction Patterns ◽  
3D Interaction ◽  
Interphase Chromosome ◽  
3D Genome ◽  
Contact Maps

AbstractGeneration of mature red blood cells, consisting mainly of hemoglobin, is a remarkable example of coordinated action of various signaling networks. Chromatin condensation is an essential step for terminal erythroid differentiation and subsequent nuclear expulsion in mammals. Here, we profiled 3D genome organization in the blood cells from ten species belonging to different vertebrate classes. Our analysis of contact maps revealed a striking absence of such 3D interaction patterns as loops or TADs in blood cells of all analyzed representatives. We also detect large-scale chromatin rearrangements in blood cells from mammals, birds, reptiles and amphibians: their contact maps display strong second diagonal pattern, representing an increased frequency of long-range contacts, unrelated to TADs or compartments. This pattern is completely atypical for interphase chromosome structure. We confirm that these principles of genome organization are conservative in vertebrate erythroid cells.

scholarly journals The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Benoît Bragantini ◽  
Christophe Charron ◽  
Maxime Bourguet ◽  
Arnaud Paul ◽  
Decebal Tiotiu ◽  
...  
Keyword(s):  
Rna Polymerase Ii ◽  
Histone H3 ◽  
Histone Chaperone ◽  
Genetic Interactions ◽  
X Ray ◽  
X Ray Crystallography ◽  
Dependent Activity ◽  
Interaction Interface ◽  
Assembly Factor ◽  
Encoding Gene

AbstractBiogenesis of eukaryotic box C/D small nucleolar ribonucleoproteins initiates co-transcriptionally and requires the action of the assembly machinery including the Hsp90/R2TP complex, the Rsa1p:Hit1p heterodimer and the Bcd1 protein. We present genetic interactions between the Rsa1p-encoding gene and genes involved in chromatin organization including RTT106 that codes for the H3-H4 histone chaperone Rtt106p controlling H3K56ac deposition. We show that Bcd1p binds Rtt106p and controls its transcription-dependent recruitment by reducing its association with RNA polymerase II, modulating H3K56ac levels at gene body. We reveal the 3D structures of the free and Rtt106p-bound forms of Bcd1p using nuclear magnetic resonance and X-ray crystallography. The interaction is also studied by a combination of biophysical and proteomic techniques. Bcd1p interacts with a region that is distinct from the interaction interface between the histone chaperone and histone H3. Our results are evidence for a protein interaction interface for Rtt106p that controls its transcription-associated activity.

scholarly journals Molecular switch from MYC to MYCN expression in MYC protein negative Burkitt lymphoma cases

2019 ◽  
Vol 9 (12) ◽  
Author(s):  
Lucia Mundo ◽  
Maria Raffaella Ambrosio ◽  
Francesco Raimondi ◽  
Leonardo Del Porro ◽  
Raffaella Guazzo ◽  
...  
Keyword(s):  
Protein Expression ◽  
Burkitt Lymphoma ◽  
Human Cancer ◽  
Large Family ◽  
World Health ◽  
Loss Of Function ◽  
Interaction Interface ◽  
Mrna And Protein Expression ◽  
Bona Fide ◽  
Health Organization

AbstractMYC is the most altered oncogene in human cancer, and belongs to a large family of genes, including MYCN and MYCL. Recently, while assessing the degree of correlation between MYC gene rearrangement and MYC protein expression in aggressive B-cell lymphomas, we observed few Burkitt lymphoma (BL) cases lacking MYC protein expression despite the translocation involving the MYC gene. Therefore, in the present study we aimed to better characterize such cases. Our results identified two sub-groups of MYC protein negative BL: one lacking detectable MYC protein expression but presenting MYCN mRNA and protein expression; the second characterized by the lack of both MYC and MYCN proteins but showing MYC mRNA. Interestingly, the two sub-groups presented a different pattern of SNVs affecting MYC gene family members that may induce the switch from MYC to MYCN. Particulary, MYCN-expressing cases show MYCN SNVs at interaction interface that stabilize the protein associated with loss-of-function of MYC. This finding highlights MYCN as a reliable diagnostic marker in such cases. Nevertheless, due to the overlapping clinic, morphology and immunohistochemistry (apart for MYC versus MYCN protein expression) of both sub-groups, the described cases represent bona fide BL according to the current criteria of the World Health Organization.

The Design of Embedded Systems GUI Message Based on Mobile and Intelligent Materials

2012 ◽  
Vol 460 ◽  
pp. 210-213
Author(s):  
Shu Zhou
Keyword(s):  
Information Technology ◽  
User Interface ◽  
Embedded Systems ◽  
Graphical User Interface ◽  
Application Framework ◽  
The Novel ◽  
Interaction Interface ◽  
Novel Method ◽  
Personal Assistant ◽  
Embedded Gui

Research and development of embedded systems is the hot information technology and the graphical user interface (GUI) is the most important part of human-computer interaction interface. These embedded GUI system has been widely used because of its strong and powerful functionality. But many times , it is necessary to sacrifice performance for its versatility. In this paper, the embedded GUI systems based on the message mechanism is study considering the short of current embedded GUI systems. The novel method of a message-driven application framework is suggested to solve the problem of the current message mechanisms for the implementation. Meanwhile, a circular queue algorithm is given in order to achieve messaging unit. Then, the message has been classified considering of efficiency. In the end, the design example of personal assistant is given.

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