scholarly journals Analysis of Eugenol Content in Ethanolic Extract of Galangal Rhizome (Alpinia galanga L. Willd) Ointment Using UV-VIS Spectrophotometry Method

2018 ◽  
Author(s):  
Adi Yugatama ◽  
Sholichah Rohmani ◽  
Rizky Apriliani
Keyword(s):  
Ethanolic Extract ◽  
Alpinia Galanga

scholarly journals Kadar hambat minimum dan kadar bunuh minimum ekstrak etanol lengkuas merah (Alpinia galanga L.) terhadap pertumbuhan Candida albicansThe minimum inhibitory concentration and a minimum lethal dose of red galangal (Alpinia galanga L.) ethanolic extract on the growth of Candida albicans

2020 ◽  
Vol 32 (1) ◽  
pp. 1
Author(s):  
Harunai Kamoda ◽  
Shelly Lelyana ◽  
Vinna Kurniawati Sugiaman
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Oral Candidiasis ◽  
Lethal Dose ◽  
Ethanolic Extract ◽  
Alpinia Galanga ◽  
Post Hoc ◽  
Minimum Lethal Dose ◽  
Colony Counter

Pendahuluan: Kandidiasis rongga mulut merupakan infeksi yang disebabkan oleh jamur patogen Candida albicans, dengan prevalensi cukup tinggi yaitu 20% - 75%. Obat antijamur yang umum digunakan untuk pengobatan kandidiasis rongga mulut adalah nistatin, akan tetapi nistatin memiliki efek samping dan harganya relatif mahal. Sebagai alternatif lain digunakan tanaman obat seperti lengkuas merah (Alpinia galanga L) yang diduga memiliki aktivitas antijamur. Tujuan penelitian ini adalah untuk mengetahui kadar hambat minimum (KHM) dan kadar bunuh minimum (KBM) ekstrak etanol lengkuas merah terhadap pertumbuhan Candida albicans. Metode: Jenis penelitian eksperimental murni dengan membandingkan kelompok uji yang mengandung ekstrak etanol lengkuas merah (alpinia galanga L) dengan konsentrasi 200mg/ml; 100mg/ml; 50mg/ml; 25mg/ml; 12,5mg/ml; 6,25mg/ml; kontrol positif berupa nistatin, dan kontrol negatif berupa DMSO 10% terhadap pertumbuhan Candida albicans dengan mengamati kekeruhan larutan pada 96 well plate menggunakan spectrophotometer  dan KBM ditentukan menggunakan colony counter dengan pengulangan sebanyak 4 kali. Analisis data menggunakan uji parametrik ANOVA dan uji Post Hoc Tuckey. Hasil: Hasil uji ANOVA nilai p 0,00 yang artinya bahwa kedua data memiliki nilai yang signifikan antar perlakuan, dan uji Post Hoc Tuckey memperlihatkan terdapat perbedaan yang signifikan pada setiap kelompok uji terhadap jumlah Candida. Simpulan: Kadar Hambat Minimum (KHM) ekstrak etanol lengkuas merah (Alpinia galanga L) terhadap pertumbuhan Candida albicancs adalah 200mg/ml namun hanya menghambat 60%, sedangkan ekstrak etanol lengkuas merah (Alpinia galanga L) tidak dapat membunuh Candida albicans.Kata kunci: Candida albicans, lengkuas merah, Alpinia galanga L. ABSTRACTIntroduction: Candida albicans is a pathogenic fungus that can cause oral candidiasis, reaches 20% - 70% prevalence in human. Antifungal drugs often used for oral candidiasis treatment is nystatin, but nystatin has many harmful side effects, and its price is relatively high. Therefore, herbal remedies such as red galangal (Alpinia galanga L.), which presumed to have antifungal activity, can be used as an alternative treatment. The purpose of this research was to determine the minimum inhibitory concentration and a minimum lethal dose of red galangal ethanolic extract on the growth of Candida albicans. Methods: This research was an experimental laboratory, conducted by comparing the test group containing ethanolic extracts of red galangal (Alpinia galanga L.) with the concentrations of 200mg/ml; 100mg/ml; 50mg/ml; 25mg/ml; 12.5mg/ml; and 6.25mg/ml; positive control group was treated with nystatin; and negative control with 10% DMSO. The solutions on 96 well plates were observed with a spectrophotometer, and the minimum killing concentration (MKC) was determined using a colony counter, and the experiment was replicated four times. The result was then analysed with ANOVA parametric test and the Post-Hoc Tuckey test afterwards. Results: The result of the ANOVA test showed that the p-value was < 0.05, which means that all treatments have a significant value, while the Post-Hoc Tuckey test indicated that there were significant differences in each group regarding the number of Candida albicans. Conclusion: Minimum Inhibitory Level (MIC) of red galangal (Alpinia galanga L) ethanolic extract against Candida albicans growth is 200mg/ml but only inhibits 60% of the growth. In contrast, the extract is unable to mortally affects the Candida albicans.Keywords: Candida albicans, red galangal, Alpinia galanga L.

scholarly journals Analgesic activity of Alpinia galanga extract in mice models and TNF-alpha receptor computational docking analysis on its leads with pharmacokinetics prediction

2018 ◽  
Vol 7 (3) ◽  
pp. 446 ◽  
Author(s):  
K. R. Subash ◽  
G. Francis Britto ◽  
Katari Sudheer Kumar ◽  
Amineni Umamaheshwari ◽  
Vijaya Chandra Reddy Konda ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Chemical Structure ◽  
Computational Analysis ◽  
Binding Free Energy ◽  
Ethanolic Extract ◽  
Standard Group ◽  
Lead Compound ◽  
Mice Model ◽  
Computational Docking ◽  
Alpinia Galanga

Background: Alpinia galanga is an ayurvedic herb recognized and used across many traditional medicine systems for its analgesic and anti-inflammatory activity. The present study scientifically validates the potential anti nociceptive action of ethanolic extract of Alpinia galanga by chemical, neurogenic and inflammatory nociception model in mice followed by identification of potential lead compound by computational analysis.Methods: The assessment of anti nociceptive action is evaluated by Acetic acid induced abdominal constrictions and Formalin assay on ethonolic extract of Alpinia galanga, followed by 20 compounds with known chemical structure of Alpinia galanga is subjected to computational analysis to predict possible lead compound with desirable pharmacokinetic and drug like features.Results: The percentage inhibition rate of Aspirin (100mg/kg) was 82.15% compared to Alpinia galanga (100mg/kg) 19.63%, (200mg/kg) 33.02% and (400mg/kg) 57.13% by acetic acid induced abdominal constrictions antinociceptive mice model. Alpinia galanga 400mg/kg (71.70%) had comparable percentage inhibition of nociception to standard group indomethacin (88.71%) in formalin induced nociceptive mice model. Among 20 compounds screened for pharmacokinetic and drug like features, Galanal B had the binding free energy -56.664 when compared to control compound 2AZ5-56.000.Conclusions: The Alpinia galanga extract had significant anti nociceptive activity and followed by computational analysis of 20 compounds with known chemical structure predicted Galanal B as lead compound with best insilico pharmacokinetic and drug like features.

Encapsulation of complex extracts in β-cyclodextrin: An application to propolis ethanolic extract

2009 ◽  
pp. 1-11 ◽  
Author(s):  
Nick Kalogeropoulos ◽  
Spyros Konteles ◽  
Ioannis Mourtzinos ◽  
Elena Troullidou ◽  
Antonia Chiou ◽  
...  
Keyword(s):  
Ethanolic Extract

Effect of Arrabidaea chica verlot ethanolic extract on epithelial cells viability exposed to a bisphosphonate

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
PMW Zago ◽  
IMO Sousa ◽  
MP Jorge ◽  
L Servat ◽  
N de C Queiroz ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Ethanolic Extract ◽  
Arrabidaea Chica
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