Genotyping of HPV DNA Positive and HPV E6/E7 mRNA Negative Cervical Samples with Abnormal Cytology

Context.—Cervical cancer screening in women younger than 30 years relies on cervical cytology because of the poor performance of human papillomavirus (HPV) DNA testing in this age group. Objectives.—To determine the performance of in-cell HPV E6, E7 mRNA quantification (HPV OncoTect) for the detection of high-grade cervical intraepithelial neoplasia in women younger than 30 years. Design.—We analyzed 3133 cytology specimens from a screening population of women aged 19–75 years investigate HPV OncoTect as a triage/secondary screening test for atypical squamous cells of undetermined significance (ASCUS) and low-grade squamous intraepithelial lesion (LSIL) cytology in women younger than 30 years. Test results were compared to histology in 246 cases. Results.—The sensitivity of E6, E7 mRNA was 89% for CIN 2+ and 100% for CIN 3+ lesions in women 30 years and older. In women younger than 30 years, the sensitivity of E6, E7 mRNA for CIN 2+ lesions was 88% for CIN 2+ and 92% for CIN 3+ lesions. Abnormal cytology (≥ASCUS) exhibited a sensitivity of 89% for CIN 2+ and 100% for CIN 3+ in women 30 years and older and 96% sensitivity for CIN 2+ and 93% sensitivity for CIN 3+ in women younger than 30. The specificity of E6, E7 mRNA was >80% for CIN 2+ and CIN 3+ in both groups of women compared to a specificity of abnormal cytology of <10% for CIN 2+ and CIN 3+ in both groups. Conclusions.—HPV OncoTect demonstrates a performance that would be effective for ASCUS/LSIL triage in women including those younger than 30 years.

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The diagnostic value of HPV E6/E7 mRNA test in young women with cervical squamous intraepithelial lesion: A Retrospective Analysis

10.21203/rs.3.rs-266984/v1 ◽

2021 ◽

Author(s):

Jing Wang ◽

Xiaomin Xu

Keyword(s):

Young Women ◽

Predictive Value ◽

Diagnostic Value ◽

Squamous Intraepithelial Lesion ◽

Abnormal Cytology ◽

Dna Test ◽

Hpv Dna ◽

Hpv E6 ◽

Intraepithelial Lesion ◽

Hpv Dna Test

Abstract Background At present, the HPV DNA test is used to triage young female patients with abnormal cytology. Still, it is not suitable to precisely identify the population with persistent HPV infection. The purpose of this study was to evaluate the diagnostic value of HPV E6/E7 mRNA test in young women with abnormal cytology by comparing HPV DNA test.Methods A total of 258 young women aged 20 to 29 years,with squamous cell abnormalities on the cervical cytology, were enrolled in this study between January 2015 and December 2019.All patients were subject to HPV DNA test, HPV E6/E7mRNA test,colposcopy biopsy, and histopathological examination.A comparative analysis of the diagnostic performance of the HPV DNA test and HPV E6/E7mRNA test was conducted according to the histological diagnosis(CIN II and CIN II+were defined as high-grade squamous intraepithelial lesion+(HSIL+)).Results The results showed that HPV E6/E7 mRNA test had a higher specificity of 47.3%(40.0%-55.1%) for HSIL+ compared to HPV DNA test that had specificity of 16.0% (11.0%-22.6%)in young women(P<0.01). The HPV E6/E7 mRNA test presented high rates of specificity,positive predictive value (PPV), and negative predictive value(NPV), which were 92.1%(86.0%-96.0%), 62.1%(42.4%-78.7%), 92.1%(85.9%-95.8%), respectively, compared to that of HPV DNA,which were 15.8%(10.4%-23.2%),14.6%(9.40%-21.9%), and 71.0%(51.8%-85.1%),respectively(P<0.01)in young women with mildly abnormal cytology(ASC-US and LSIL). Yet, with severe abnormal cytology(ASC-H and HSIL), HR-HPV test was similar to HPV E6/E7 mRNA test in sensitivity(χ2=0.98,P=0.322),specificity(χ2=0.938,P=0.333),PPV(χ2=0.074,P=0.786) and NPV(χ2=0.00,P=1.000).Conclusions Compared to the HPV DNA test,the HPV E6 /E7mRNA test has better clinical value in screening cervical cancer and predicting the risk of HSIL+ in young women, especially those with mild abnormal cytology.

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Genotyping of HPV DNA positive and HPV E6/E7 mRNA negative cervical samples with abnormal cytology

Journal of Clinical Virology ◽

10.1016/j.jcv.2016.08.175 ◽

2016 ◽

Vol 82 ◽

pp. S88

Author(s):

A. Altay ◽

I. Tuney ◽

K. Ergunay ◽

A. Usubutun ◽

K. Yuce ◽

...

Keyword(s):

Abnormal Cytology ◽

Hpv Dna ◽

Hpv E6

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Evaluation of HPV16 E7 expression in head and neck carcinoma cell lines and clinical specimens

Scientific Reports ◽

10.1038/s41598-020-78345-8 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Koji Kitamura ◽

Keisuke Nimura ◽

Rie Ito ◽

Kotaro Saga ◽

Hidenori Inohara ◽

...

Keyword(s):

Head And Neck ◽

Cell Lines ◽

Carcinoma Cell ◽

Head And Neck Carcinoma ◽

Oropharyngeal Carcinoma ◽

Human Embryonic Kidney Cells ◽

Clinical Specimens ◽

Hpv Dna ◽

Hpv E6 ◽

Carcinoma Cell Lines

AbstractHuman papillomavirus (HPV) 16 infection in the oropharynx is one of the major risk factors for oropharyngeal carcinoma. Although the HPV E6 and E7 proteins are known to have a role in head and neck carcinogenesis, whether their expression is maintained once the tumour has developed still remains unclear. We evaluated the expression of these proteins in HPV16-positive cancer cell lines and clinical oropharyngeal specimens. Two out of the four commercially available antibodies directed against the E7 protein could detect the E7 protein overexpressed in the 293FT cells, human embryonic kidney cells, although none of the four commercially available anti-E6 antibodies could detect the overexpressed E6 protein. Whereas HPV16-positive head and neck or cervical carcinoma cell lines expressed the E7 mRNA, the antibodies with an ability to detect the E7 protein could not detect it in western blotting in these HPV16-positive cell lines. In clinical specimens, E7 protein was partially detected in p16-positive area in p16-positive and HPV16 DNA-positive samples, but not in p16-negative and HPV DNA-negative or p16-positive and HPV DNA-negative samples. Consistent with these findings, the E7 protein was poorly translated from the endogenous structure of the E7 mRNA, although significant E7 mRNA expression was detected in these samples. Our findings indicate that E7 protein is partially expressed in p16-positive area in p16-positive and HPV16 DNA-positive clinical specimens.

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Diagnostic Performance of HPV E6/E7 mRNA and HPV DNA Assays for the Detection and Screening of Oncogenic Human Papillomavirus Infection among Woman with Cervical Lesions in China

Asian Pacific Journal of Cancer Prevention ◽

10.7314/apjcp.2015.16.17.7633 ◽

2015 ◽

Vol 16 (17) ◽

pp. 7633-7640 ◽

Cited By ~ 9

Author(s):

Hye-young Wang ◽

Dongsup Lee ◽

Sunyoung Park ◽

Geehyuk Kim ◽

Sunghyun Kim ◽

...

Keyword(s):

Human Papillomavirus ◽

Diagnostic Performance ◽

Cervical Lesions ◽

Hpv Dna ◽

Hpv E6 ◽

Human Papillomavirus Infection ◽

Papillomavirus Infection ◽

Dna Assays

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Prevalence of abnormal cervical cytology and HPV DNA positivity among HIV positive women

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20193791 ◽

2019 ◽

Vol 8 (9) ◽

pp. 3649

Author(s):

Sunita Malik ◽

Supriti Kumari ◽

Harsha S. Gaikwad ◽

Archana Mishra ◽

Mausumi Bharadwaj

Keyword(s):

Hpv Infection ◽

Cervical Cytology ◽

Hiv Positive ◽

Abnormal Cytology ◽

Cross Sectional ◽

Hpv Dna ◽

Hpv Dna Testing ◽

Abnormal Cervical Cytology ◽

Hiv Positive Women ◽

Relationship Of

Background: The relationship among HIV, HPV, and development of CIN is complex and incompletely understood. Present study is undertaken to find out the prevalence and relationship of abnormal cervical cytology and HPV infection in HIV positive women.Methods: This was a cross-sectional, case control study conducted on 95 HIV seropositive and 95 seronegative women. Specimen was collected from the cervix for HPV DNA testing, subtyping and cytology.Results: HPV DNA positivity was higher in seropositive group (18.6% vs. 7.4%). Premalignant conditions were found only in seropositive group. At CD4 count <249 HPV DNA positivity was 53%, at 250-499 the percentage of HPV DNA positivity was 31% and at >500 HPV DNA positivity was 19%.Conclusions: Prevalence of abnormal cytology and HPV DNA positivity is higher amongst HIV positive women and there is an association between HPV DNA positivity with lower CD4 counts.

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Sensitivity, Specificity, and Clinical Value of Human Papillomavirus (HPV) E6/E7 mRNA Assay as a Triage Test for Cervical Cytology and HPV DNA Test

Journal of Clinical Microbiology ◽

10.1128/jcm.02570-10 ◽

2011 ◽

Vol 49 (7) ◽

pp. 2643-2650 ◽

Cited By ~ 63

Author(s):

Maria Benevolo ◽

Amina Vocaturo ◽

Donatella Caraceni ◽

Deborah French ◽

Sandra Rosini ◽

...

Keyword(s):

Human Papillomavirus ◽

Cervical Cytology ◽

Clinical Value ◽

Dna Test ◽

Hpv Dna ◽

Hpv E6 ◽

Sensitivity Specificity ◽

Triage Test ◽

Hpv Dna Test

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Prevalence of Chlamydia trachomatis Infection, Serovar Distribution and Co-Infections with Seven High-Risk HPV Types among Italian Women with a Recent History of Abnormal Cervical Cytology

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16183354 ◽

2019 ◽

Vol 16 (18) ◽

pp. 3354 ◽

Cited By ~ 3

Author(s):

Marianna Martinelli ◽

Rosario Musumeci ◽

Alberto Rizzo ◽

Narcisa Muresu ◽

Andrea Piana ◽

...

Keyword(s):

Chlamydia Trachomatis ◽

Screening Program ◽

Hpv Infection ◽

Cervical Cytology ◽

Recent History ◽

Abnormal Cytology ◽

Chlamydia Trachomatis Infection ◽

Hpv Dna ◽

Abnormal Cervical Cytology ◽

History Of

Chlamydia trachomatis (Ct) and human papillomavirus (HPV) are the most prevalent sexually transmitted infections throughout the world. Despite the serious complications associated with chronic Ct infections in sexually active women, a screening program is not yet available in Italy. Moreover, HPV/Ct co-infections are also known to occur frequently, increasing the risk of HPV-induced carcinogenesis. The aim of this study was to evaluate the prevalence of Ct infections, the distribution of Ct serovars, and the incidences of Ct/HPV co-infections among women with a recent history of abnormal cervical cytology. Cervical samples were collected from 199 women referred for a gynecological visit following an abnormal Pap test results. All samples were tested for the presence of Ct and HPV DNA using real-time PCR assays; Ct typing of positive samples was performed by PCR–RFLP (restriction fragment length polymorphism) targeting the ompA gene. A high percentage of these women (12.8% and 21.7% with or without abnormal cytology on “retesting”, respectively) were found to be Ct positive. Serovar F was the most prevalent type in Ct positive women, followed by E and K. Ct/HPV co-infections were detected in 7% (14/199) of enrolled women, with HPV-16, HPV-51, and HPV-52 being most frequently identified in co-infections. This study provides new epidemiological data on the prevalence of Ct and associated HPV infection in women with a recent history of abnormal cervical cytology in Italy, where notification of cases is not mandatory.

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8. Human papillomavirus mRNA testing for the detection of anal high-grade squamous intraepithelial lesions in HIV-positive men who have sex with men

Sexual Health ◽

10.1071/shv10n6ab8 ◽

2013 ◽

Vol 10 (6) ◽

pp. 573

Author(s):

Elena Sendagorta Cudós ◽

Maria P. Romero Gomez ◽

Beatriz Hernandez Novoa ◽

Ander Mayor ◽

Jose I. Bernardino De La Serna ◽

...

Keyword(s):

Predictive Value ◽

Screening Program ◽

University Hospital ◽

High Grade ◽

Squamous Intraepithelial Lesions ◽

Cross Sectional ◽

Hpv Dna ◽

Hpv E6 ◽

Anal Cytology ◽

Intraepithelial Lesions

Background Currently, screening for anal high-grade squamous intraepithelial lesions (HSIL) relies on anal cytology and high-resolution anoscopy (HRA). Since this approach has limited sensitivity and specificity for detecting anal HSIL, there is increasing interest in the role of biomarkers for predicting anal HSIL. The aim of this study is to evaluate the diagnostic accuracy of HPV E6/E7-mRNA expression for the detection of anal HSIL in MSM HIV-infected patients, in comparison to DNA-HR-HPV and anal cytology. Methods: This cross-sectional screening study included 101 MSM followed at the HIV-unit of La Paz University Hospital. Intra-anal swabs from patients participating in a screening program including cytology, HRA and histology were analysed. HR-HPV-DNA detection was performed by means of the CLART HPV2 assay (GENOMICA SAU.). E6/E7-mRNA detection of HR-HPV types 16, 18, 31, 33 and 45 was performed using the NucliSENS-EasyQ assay (BioMérieux, Marcy l’Etoile, France). Results: HR-HPV DNA and HPV E6/E7 mRNA were detected in 82% and 57% of the anal smears, respectively. Anal cytology screening was abnormal in 70.3%. For the detection of HSIL sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were 71.7%, 55.6%, 57.9%, and 69.8% for E6/E7-mRNA testing, respectively, compared with 97.9%, 31.5%, 55.4% and 94.4%, respectively, for HR-HPV DNA testing and 83%, 40.7%, 54.9%, 73.3%, respectively, for cytology testing. Conclusions: In comparison with the other tests, the NucliSENS EasyQ HPV assay yielded a lower clinical sensitivity but a higher clinical specificity and PPV for the detection of anal HSIL in MSM HIV-infected patients.

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