scholarly journals Validation of the spectrophotometric method for the dosing of some combined capsule

2021 ◽  
Vol 64 (4) ◽  
pp. 10-16
Author(s):  
Livia Uncu ◽  
◽  
Vladilena Evtodienco ◽  
Ecaterina Mazur ◽  
Elena Donici ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Limit Values ◽  
Minimum Error ◽  
Quantification Limit ◽  
Relative Standard ◽  
Validation Parameters ◽  
High Degree

Background: UV-Vis spectrophotometry remains the most accessible spectral method with a high degree of sensitivity and information. The advantage of the method consists in its universality, the ability to combine with other methods, the minimum error, as well as its economic efficiency. The objective of this study was the determination of some validation parameters for the spectrophotometric method of dosing piracetam and nicergoline in combined capsules. Material and methods: Agilent 8453 UV-Vis spectrophotometer, reference standards of piracetam and nicergoline, 0.1 M HCl methanolic solution. Validation of the spectrophotometric method according to the requirements of the ICH guide “Q2R1: For analytical procedures and validation”. Results: Linearity was investigated on concentration ranges 5-40 µg / mL. The regression (R2 ) values were 0.9998 for nicergoline and 0.998 for piracetam, respectively. The limit of detection was 1.737 µg / mL for nicergoline and 0.369 µg / mL for piracetam. Quantification limit values were also calculated as 5.265 and 1.118 µg / mL for nicergoline and piracetam, accordingly. The results obtained showed that the developed spectrophotometric method is accurate, precise and robust, because the value of the relative standard deviation was less than 1.0%. Conclusions: The developed spectrophotometric method showed specificity, linearity, accuracy, precision and robustness, and can be applied on the concentration range between 80-120% of the nominal value of the content of nicergoline and piracetam in the preparation.

scholarly journals Determination of Sodium Cromoglycate by a New Kinetic Spectrophotometric Method in Biological Samples

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Mohsen Keyvanfard ◽  
Khadijeh Alizad ◽  
Razieh Shakeri
Keyword(s):  
Standard Deviation ◽  
Sodium Cromoglycate ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Biological Samples ◽  
Limit Of Detection ◽  
Micellar Medium ◽  
Relative Standard ◽  
Kinetic Spectrophotometric

A new kinetic spectrophotometric method is described for the determination of ultratrace amounts of sodium cromoglycate (SCG). The method based on catalytic action of SCG on the oxidation of amaranth with periodate in acidic and micellar medium. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance of the amaranth at 518 nm, for the first 4 min from initiation of the reaction. Calibration curve was linear in the range of 4.0−36.0 ng mL−1SCG. The limit of detection is 2.7 ng mL−1SCG. The relative standard deviation (RSD) for ten replicate analyses of 12, 20, and 28 ng mL−1SCG was 0.40%, 0.32%, and 0.53%, respectively. The proposed method was used for the determination of SCG in biological samples.

Kinetic Spectrophotometric Determination of Ruthenium by its Catalytic Effect on the Reduction of Spadns with Sodium Hypophosphite in Micellar Media

2012 ◽  
Vol 204-208 ◽  
pp. 4067-4070 ◽  
Author(s):  
Zhi Rong Zhou ◽  
Li Zhen Zhang
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Catalytic Effect ◽  
Limit Of Detection ◽  
Fixed Time ◽  
Sodium Hypophosphite ◽  
Micellar Media ◽  
Relative Standard ◽  
Kinetic Spectrophotometric

A simple kinetic spectrophotometric method was developed for the determination of trace amounts of Ru (III). The method is based on the reduction of spadns by sodium hypophosphite (NaH2PO2) in micellar media. The reaction was monitored spectrophotometrically by measuring the decrease in the absorbance of spadns at 515 nm with a fixed-time method. The decrease in the absorbance of spadns is proportional to the concentration of Ru (III) in the range 0.40–10.0 μg/L with a fixed time of 2.5–7.0 min from the initiation of the reaction. The limit of detection is 0.12 μg/L Ru (III). The relative standard deviation for the determination of 0.10 and 0.20 μg/25mL Ru (III) was 2.3 % and 2.0 %, respectively. The method was applied to the determination of Ru (III) in some ores and metallurgy products.

scholarly journals Flotation-Spectrophotometric Method for the Determination of Aluminium ion Using Xylenol Orange

2011 ◽  
Vol 8 (4) ◽  
pp. 1528-1535 ◽  
Author(s):  
F. Nekouei ◽  
Sh. Nekouei
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Limit Of Detection ◽  
Xylenol Orange ◽  
Molar Absorptivity ◽  
Relative Standard ◽  
Ion Associate ◽  
Sensitive Method

A simple, fast, reproducible and sensitive method for the flotation- spectrophotometric determination of Al3+is reported. The apparent molar absorptivity (ε) of the ion associate was determined to be 8.35×104L mol-1cm-1. The calibration curve was linear in the concentration range of 1.0-50 ng mL-1of Al3+with a correlation coefficient of 0.9997. The limit of detection (LOD) was 0.621 ng mL. The relative standard deviation (RSD) at 10 and 30 ng mL-1of aluminium were 1.580 and 2.410% (n=7) respectively. The method was applied for measuring the amount of aluminium in water samples.

scholarly journals SPECTROPHOTOMETRIC DETERMINATION OF CEFPIROME IN PHARMACEUTICAL PREPARATION

2020 ◽  
pp. 124-127
Author(s):  
DILIP M CHAFLE
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Pharmaceutical Preparation ◽  
Effect Of Temperature ◽  
Percentage Error ◽  
Ferric Nitrate ◽  
Red Color ◽  
Relative Standard

Objective: A simple, sensitive and precise visible spectrophotometric method has been proposed for the determination of cefpirome (CFM) in pure and oral injectable dosage form. Methods: A spectrophotometric method is based on the formation of stable red color product by oxidation of drugs by ferric nitrate and subsequent complexation with 1, 10 – phenanthroline with maximum absorption at 515 nm. Result: The red color complex was formed between Fe (II) and 1, 10 – phenanthroline after reduction of Fe (III) to Fe (II) in the presence of CFM drug. The phosphoric acid solution was used only for quenching the complex formation reaction. Several parameters such as the maximum wavelength of absorption, the volume of reagents, sequence of addition and effect of temperature and time of heating were optimized to achieve high sensitivity, stability and reproducible results. Under the optimum conditions, linear relationship with good correlation coefficient (0.994) was found over the concentration range from 0.20 to 6.00 μg/mL with a molar extinction coefficient 7.7813 × 104 L/mol/cm, limit of detection 0.2026 and limit of quantification 0.6141 μg/mL, respectively. Conclusion: The proposed method was evaluated statistically for linearity, accuracy, and precision in terms of standard deviation, percentage recovery, percentage error and relative standard deviation. The proposed method can be applied for the routine estimation of CFM in the laboratory.

scholarly journals ULTRAVIOLET-SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF NORFLOXACIN PRESENT IN TASTE MASKED DRUG RESIN COMPLEX

2018 ◽  
Vol 11 (4) ◽  
pp. 244
Author(s):  
Ayya Rajendra Prasad ◽  
Jayanthi Vijaya Ratna
Keyword(s):  
Spectrophotometric Method ◽  
Absorption Maximum ◽  
Method Development ◽  
Limit Of Detection ◽  
Statistical Parameters ◽  
Limit Of Quantification ◽  
Relative Standard ◽  
Validation Parameters ◽  
Development And Validation

 Objective: The objective of this study was developed and validated a novel, specific, precise, and simple ultraviolet (UV)-spectrophotometric method for the estimation of norfloxacin present in taste masked drug-resin complex.Methods: UV-spectrophotometric determination was performed with ELICO SL 1500 UV-visible spectrophotometer using 0.1 N HCl as a medium. The spectrum of the standard solution was run from 200 to 400 nm range for the determination of absorption maximum (λ max). λ max of norfloxacin was found at 278 nm. The absorbance of standard solutions of 1, 2, 3, 4, and 5 μg/ml of drug solution was measured at an absorption maximum at 278 nm against the blank. Then, a graph was plotted by taking concentration on X-axis and absorbance on Y-axis which gave a straight line. Validation parameters such as linearity and range, selectivity and specificity, limit of detection (LOD) and limit of quantification (LOQ), accuracy, precision, and robustness were evaluated as per the International Conference on Harmonization (ICH) guidelines.Results: Linearity for the UV-spectrophotometric method was noted over a concentration range of 1–5 μg/ml with a correlation coefficient of 0.9995. The LOD and LOQ for norfloxacin were found at 0.39 μg/ml and 1.19 μg/ml, respectively. Accuracy was in between 99.00% and 99.17%. % relative standard deviation for repeatability, intraday precision, and interday precision was found to be 0.600, in between 0.291 and 0.410, and in between 0.682 and 1.439, respectively. The proposed UV spectrophotometric method is found to be robust.Conclusion: The proposed UV-spectrophotometric method was validated according to the ICH guidelines, and results and statistical parameters demonstrated that the developed method is sensitive, precise, reliable, and simple for the estimation of norfloxacin present in taste masked drug-resin complex.

scholarly journals Spectrophotometric Method for the Determination of Drugs Containing Phenol Group by Using 2, 4- Dinitrophenylhydrazine

2010 ◽  
Vol 7 (2) ◽  
pp. 395-402
Author(s):  
Padmarajaiah Nagaraja ◽  
Ashwinee Kumar Shrestha
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Dosage Forms ◽  
Limit Of Quantification ◽  
Pharmaceutical Dosage Forms ◽  
Accuracy And Precision ◽  
Relative Standard ◽  
Phenolic Drugs

A spectrophotometric method has been proposed for the determination of four phenolic drugs; salbutamol, ritodrine, amoxicillin and isoxsuprine. The method is based on the oxidation of 2, 4- dinitrophenyl-hydrazine and coupling of the oxidized product with drugs to give intensely colored chromogen. Under the proposed optimum condition, beer’s law was obeyed in the concentration range of 2.5-17, 2-29, 4-33 and 5-30 μg/mL for salbutamol, ritodrine, amoxicillin and isoxsuprine respectively. The limit of detection (LOD) and limit of quantification (LOQ) were 0.2, 0.83, 0.09, 0.84 μg/mL and 0.66, 2.79, 0.3 and 2.81 μg/mL in the same order. No interference was observed from common pharmaceutical adjuvants. The ringbom plots and low relative standard deviation assert the applicability of this method. The suggested method was further applied for the determinations of drugs in commercial pharmaceutical dosage forms, which was compared statistically with reference methods by means oft- test andF- test and were found not to differ significantly at 95% confidence level. The procedure is characterized by its simplicity with accuracy and precision.

scholarly journals MEAN CENTERING OF RATIO SPECTRA METHOD FOR THE ANALYSIS OF THEOPHYLLINE AND EPHEDRINE HCL MIXTURE IN TABLET

2018 ◽  
Vol 11 (5) ◽  
pp. 218
Author(s):  
Muchlisyam Muchlisyam ◽  
Sudarmi Sudarmi ◽  
Cindy Caroline
Keyword(s):  
Standard Deviation ◽  
Detection Limit ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Standard Addition ◽  
Addition Method ◽  
Limit Of Quantification ◽  
Mean Centering ◽  
Relative Standard

 Objective: Mean centering of ratio spectra method (MCR method) is one of the simplest methods for the determination of drug mixtures. The purpose of this research is to determine the content of theophylline (THEO) and ephedrine HCl (EPH) in tablets by MCR spectra method.Methods: This research was conducted with the MCR method. It was measured at 271 nm for THEO and 257 nm for EPH using 0.1 N HCL as a solution. The calculation was conducted with Matlab application. The analytical characteristics of the method are detection limit, accuracy, precision, and selectivity. Standard addition method was used to increase the concentration of EPH in the sample until it reached the range of calibration concentration.Result: The research has showed that validations for THEO were 100.57% for accuracy, 0.68% for relative standard deviation (RSD), 0.46 μg/mL for limit of detection (LOD), and 1.52 μg/mL for limit of quantification (LOQ). Meanwhile, the validations for EPH are 100.02% for accuracy, 0.07% for RSD, 43.12 μg/mL for LOD, and 143.72 μg/mL for LOQ. The level of THEO is 97.43 ± 0.17% and the level of EPH is 101.36 ± 0.25% for brand one’s tablet. Meanwhile, brand two’s tablet contains 98.72 ± 0.14% of THEO and 103.62 ± 0.23% of EPH.Conclusion: MCR ultraviolet spectrophotometric method can be used to determine the content of THEO and EPH in tablets and meets the detection limit, accuracy, precision, and selectivity.

scholarly journals The Development of a New Kinetic Spectrophotometric Method for the Determination of Vanadium(V) Based on its Catalytic Effect on the Oxidation of Malachite Green Oxalate by Bromate in Acidic and Micellar Medium

2010 ◽  
Vol 7 (4) ◽  
pp. 1612-1620 ◽  
Author(s):  
M. Keyvanfard ◽  
N. Abedi
Keyword(s):  
Malachite Green ◽  
Spectrophotometric Method ◽  
Catalytic Effect ◽  
Limit Of Detection ◽  
Fixed Time ◽  
Micellar Medium ◽  
Relative Standard ◽  
Kinetic Spectrophotometric ◽  
Malachite Green Oxalate

A new, simple, sensitive and selective kinetic spectrophotometric method was developed for the determination of ultra trace amounts of vanadium(V). The method is based on the catalytic effect of vanadium(V) on the oxidation of malachite green oxalate (MG) by bromate in acidic and micellar medium. The reaction was monitored spectrophotometrically by measuring the decrease in the absorbance of malachite green oxalate (MG) at 625 nm with a fixed-time method. The decrease in the absorbance of MG is proportional to the concentration of vanadium(V) in the range of 1-100 ng/mL with a fixed time of 0.5-2 min from the initiation of the reaction. The limit of detection is 0.71 ng/mL of vanadium(V). The relative standard deviation for the determination of 5, 30, 50 ng/mL of vanadium(V) was2.5% 2.6%, 2.4% and respectively. The method was applied to the determination of vanadium(V) in water samples.

scholarly journals Development and validation of spectrophotometric method for phenylephrine hydrochloride estimation in nasal drops formulations

2008 ◽  
Vol 27 (2) ◽  
pp. 149 ◽  
Author(s):  
Ivana Savić ◽  
Goran Nikolić ◽  
Vladimir Banković
Keyword(s):  
Standard Deviation ◽  
Sodium Hydroxide ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Molar Absorptivity ◽  
Dosage Forms ◽  
Phenylephrine Hydrochloride ◽  
Relative Standard ◽  
Development And Validation

Simple, accurate and reproducible UV-spectrophotometric method was developed and validated for the estimation of phenylephrine hydrochloride in pharmaceutical nasal drops formulations. Phenylephrine hydrochloride was estimated at 291 nm in 1 mol⋅dm-3 sodium hydroxide (pH 13.5). Beer’s law was obeyed in the concentration range of 10–100 μg⋅cm−3 (r2 = 0.9990) in the sodium hydroxide medium. The apparent molar absorptivity was found to be 1.63×103 dm3⋅mol−1⋅cm−1. The method was tested and validated for various parameters according to the ICH (International Conference on Harmonization) guidelines. The detection and quantitation limits were found to be 0.892 and 2.969 μg⋅cm−3, respectively. The proposed method was successfully applied for the determination of phenylephrine hydrochloride in pharmaceutical nasal drops formulations. The results demonstrated that the procedure is accurate, precise and reproducible (relative standard deviation < 1 %), while being simple, cheap and less time consuming, and hence can be suitably applied for the estimation of phenylephrine hydrochloride in different dosage forms.

SIMULTANEOUS ESTIMATION OF DOXORUBICIN AND HYDROXYCHAVICOL IN BULK AND PHARMACEUTICAL FORMULATION BY Q-ABSORPTION RATIO METHOD

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (02) ◽  
pp. 51-58
Author(s):  
Sachin Bhusari ◽  
Bharti Gotmare ◽  
Pravin Wakte
Keyword(s):  
Quality Control ◽  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Pharmaceutical Formulation ◽  
Ratio Method ◽  
Simultaneous Estimation ◽  
Calibration Standards ◽  
Analytical Method Validation ◽  
Relative Standard ◽  
Validation Parameters

A simple, rapid, accurate, precise and economical UV-spectrophotometric method has been developed and validated for simultaneous estimation of doxorubicin hydroxychavicol (DOXO) and OH-CHV in a pharmaceutical formulation. It is based on the Q-analysis UV Spectrophotometric method. The absorption spectra of DOXO and OH-CHV were obtained over the range of 200-800 nm, and the absorption maxima were determined. Multiple calibration standards were prepared for both the drugs separately and absorbances were recorded at respective absorption maxima. Calibration curves were plotted and the linear responses were studied. Various analytical method validation parameters viz. accuracy, precision, LOD, LOQ, robustness and ruggedness were calculated using quality control standards. The absorbance maxima of DOXO and OH-CHV were found to be 477 nm and 284 nm, respectively in methanol. In Q-analysis, the isoabsorptive point for both the drugs was found at 297 nm. The linearity range was between 5-40 μg/ml for DOXO and 5-40 μg/ml for OH-CHV at their respective wavelengths. The intra-day and inter-day study shows percent relative standard deviation in the range of 0.9991 to 0.9993. LOD and LOQ were found to be 0.024 µg/ml and 0.075µg/ml for DOXO and 0.228μg/ml and 0.692μg/ml for OH-CHV. The total percent recoveries of DOXO and OH-CHV were found to be 98.75 and 99.41, respectively.

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