New Findings in Non-invasive Assessment of Embryo Quality

2021 ◽  
Vol 12 (3) ◽  
pp. 206-219
Author(s):  
O Badri seighaldehi ◽  
H Mohseni Kouchesfahani ◽  
N Nasiri ◽  
M Shahverdi ◽  
M Hezavehei ◽  
...  
Keyword(s):  
Embryo Quality ◽  
Non Invasive ◽  
New Findings

Non-invasive biomarkers for embryo quality in sterile patients

2018 ◽  
Author(s):  
Iris Martínez Rodero ◽  
Juan Manuel Moreno Moya ◽  
Josep Marí-Alexandre ◽  
Irene Cuevas Saiz ◽  
Carla Olmedo Illueca
Keyword(s):  
Embryo Quality ◽  
Non Invasive

scholarly journals Analysis of Morphokinetic Parameters of Feline Embryos Using a Time-Lapse System

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 748
Author(s):  
Joanna Kochan ◽  
Agnieszka Nowak ◽  
Barbara Kij ◽  
Sylwia Prochowska ◽  
Wojciech Niżański
Keyword(s):  
Embryo Development ◽  
Embryo Quality ◽  
Time Lapse ◽  
Blastocyst Stage ◽  
Cavity Formation ◽  
Non Invasive ◽  
Morphokinetic Parameters ◽  
Morphological Defects ◽  
Development In Vitro

The aim of this study was to analyze the morphokinetic parameters of feline embryos using a time lapse system. Oocytes matured in vitro were fertilized (IVF) and in vitro cultured in a time lapse-system (Primo Vision®, Gothenburg, Sweden). The first cell division of embryos occurred between 17 h post insemination (hpi) and 38 hpi, with the highest proportion of embryos (46%) cleaving between 21 and 24 hpi. The timing of the first cleavage significantly affected further embryo development, with the highest development occurring in embryos that cleaved at 21–22 hpi. Embryos that cleaved very early (17–18 hpi) developed poorly to the blastocyst stage (2%) and none of the embryos that cleaved later than 27 hpi were able to reach the blastocyst stage. Morphological defects were observed in 48% of the embryos. There were no statistically significant differences between the timing intervals of the first cleavage division and the frequency of morphological defects in embryos. Multiple (MUL) morphological defects were detected in more than half (56%) of the abnormal embryos. The most frequent single morphological defects were cytoplasmic fragmentation (FR) (8%) and blastomere asymmetry (AS) (6%). Direct cleavage (DC) from 1–3 or 3–5 blastomeres, reverse cleavage (RC) and vacuoles were rarely observed (2–3%). The timing of blastocyst cavity formation is a very good indicator of embryo quality. In our study, blastocyst cavity formation occurred between 127–167 hpi, with the highest frequency of hatching observed in blastocysts that cavitated between 142–150 hpi. Blastocysts in which cavitation began after 161 h did not hatch. In conclusion, the timing of the first and second cleavage divisions, the timing of blastocyst cavity formation and morphological anomalies can all be used as early and non-invasive indicators of cat embryo development in vitro.

Cell-free DNA: a non-invasive test for assessing embryo quality

Placenta ◽  
2011 ◽  
Vol 32 ◽  
pp. S281-S282 ◽  
Author(s):  
Paola Scaruffi ◽  
Shanti Levi ◽  
Gian Paolo Tonini ◽  
Paola Anserini
Keyword(s):  
Embryo Quality ◽  
Cell Free Dna ◽  
Non Invasive ◽  
Invasive Test ◽  
Free Dna

scholarly journals Respiration rates of individual bovine in vitro-produced embryos measured with a novel, non-invasive and highly sensitive microsensor system

Reproduction ◽  
2005 ◽  
Vol 130 (5) ◽  
pp. 669-679 ◽  
Author(s):  
A S Lopes ◽  
L H Larsen ◽  
N Ramsing ◽  
P Løvendahl ◽  
M Räty ◽  
...  
Keyword(s):  
Respiration Rate ◽  
Embryo Quality ◽  
Developmental Stages ◽  
Embryo Morphology ◽  
The Novel ◽  
Non Invasive ◽  
Respiration Rates ◽  
Morphological Evaluation ◽  
Highly Sensitive

Oxygen consumption is a useful parameter for evaluating embryo quality, since it provides a valuable indication of overall metabolic activity. Over the years, several approaches have been used to measure the respiration rates of individual embryos, but a convincing method has not yet been reported. In this study, we introduce and have validated a novel high resolution microsensor technology to determine the respiration rates of individual embryos at different developmental stages. We have employed this technology to investigate the correlation between respiration rate and embryo morphology, diameter and sex. Following morphological evaluation, individual respiration rates of day 3 (n = 18) and day 7 (n = 60) bovine in vitro-produced embryos were determined. Of the measured embryos, 64 were lysed for sex diagnosis by PCR. Average respiration rates of day 7 embryos (1.30 ± 0.064 nl/h) were 3.4-fold higher than day 3 embryos (0.38 ± 0.011 nl/h). On day 7, the average respiration rate of quality 1 blastocysts was significantly higher than the respiration rates of the lower qualities. For both day 3 and day 7 embryos, respiration rates were directly influenced by embryo diameter but did not differ between sexes. These results have demonstrated that the novel microsensor technology can be used to accurately and rapidly (8 min) measure the respiration rates of individual embryos at different developmental stages. Respiration rates were only in partial agreement with embryo morphology, suggesting a slight discrepancy between these two methods in assessing embryo quality. It is likely that a combined assessment of embryo respiration and morphology would improve embryo classification and subsequent selection.

scholarly journals Micro- and Nano-Scales Three-Dimensional Characterisation of Softwood

2021 ◽  
Vol 7 (12) ◽  
pp. 263
Author(s):  
Alessandra Patera ◽  
Anne Bonnin ◽  
Rajmund Mokso
Keyword(s):  
Mechanical Response ◽  
Three Dimensional ◽  
Deep Understanding ◽  
Swelling Behaviour ◽  
Multi Scale ◽  
Non Invasive ◽  
X Ray Imaging ◽  
New Findings ◽  
Bordered Pits ◽  
Paul Scherrer

Understanding the mechanical response of cellular biological materials to environmental stimuli is of fundamental importance from an engineering perspective in composites. To provide a deep understanding of their behaviour, an exhaustive analytical and experimental protocol is required. Attention is focused on softwood but the approach can be applied to a range of cellular materials. This work presents a new non-invasive multi-scale approach for the investigation of the hygro-mechanical behaviour of softwood. At the TOMCAT beamline of the Paul Scherrer Institute, in Switzerland, the swelling behaviour of softwood was probed at the cellular and sub-cellular scales by means of 3D high-resolution phase-contrast X-ray imaging. At the cellular scale, new findings in the anisotropic and reversible swelling behaviour of softwood and in the origin of swelling hysteresis of porous materials are explained from a mechanical perspective. However, the mechanical and moisture properties of wood highly depend on sub-cellular features of the wood cell wall, such as bordered pits, yielding local deformations during a full hygroscopic loading protocol.

scholarly journals Label-free assessment of pre-implantation embryo quality by the Fluorescence Lifetime Imaging Microscopy (FLIM)-phasor approach

2018 ◽  
Author(s):  
Ning Ma ◽  
Nabora Reyes de Mochel ◽  
Paula Duyen Anh Pham ◽  
Tae Yeon Yoo ◽  
Ken WY. Cho ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Embryo Quality ◽  
Assisted Reproductive Technologies ◽  
Learning Algorithm ◽  
Reproductive Technologies ◽  
List Type ◽  
Label Free ◽  
Embryo Viability ◽  
Invasive Approach ◽  
Non Invasive

AbstractDevelopment of quantitative, safe and rapid techniques for assessing embryo quality provides significant advances in Assisted Reproductive Technologies (ART). We apply the phasor-FLIM method to capture endogenous fluorescent biomarkers of pre-implantation embryos as a non-morphological caliber for embryo quality. Here, we identify the developmental, or “D-trajectory”, that consists of fluorescence lifetime from different stages of mouse pre-implantation embryos. The D-trajectory correlates with intrinsic fluorescent species from a distinctive energy metabolism and oxidized lipids, as seen with Third Harmonic Generation (THG) that changes over time. In addition, we have defined an Embryo Viability Index (EVI) to distinguish pre-implantation embryo quality using the Distance Analysis, a machine learning algorithm to process the fluorescence lifetime distribution patterns. We show that the phasor-FLIM approach provides a much-needed non-invasive quantitative technology for identifying healthy embryos at the early compaction stage with 86% accuracy. This may increase embryo implantation success for in vitro fertilization clinics.HighlightsA label-free method of tracking metabolic trajectories during pre-implantation mouse embryo development.A non-invasive approach for assessing embryo quality and viability by a phasor-FLIM analysis.

scholarly journals Non-Invasive Continuous Measurement of Haemodynamic Parameters—Clinical Utility

2021 ◽  
Vol 10 (21) ◽  
pp. 4929
Author(s):  
Aleksandra Bodys-Pełka ◽  
Maciej Kusztal ◽  
Maria Boszko ◽  
Renata Główczyńska ◽  
Marcin Grabowski
Keyword(s):  
Clinical Utility ◽  
Everyday Clinical Practice ◽  
Non Invasive ◽  
Haemodynamic Parameters ◽  
Cardiac Procedures ◽  
New Findings ◽  
Evaluation And Monitoring ◽  
Invasive Techniques ◽  
Further Development ◽  
Invasive Measurement

The evaluation and monitoring of patients’ haemodynamic parameters are essential in everyday clinical practice. The application of continuous, non-invasive measurement methods is a relatively recent solution. CNAP, ClearSight and many other technologies have been introduced to the market. The use of these techniques for assessing patient eligibility before cardiac procedures, as well as for intraoperative monitoring is currently being widely investigated. Their numerous advantages, including the simplicity of application, time- and cost-effectiveness, and the limited risk of infection, could enforce their further development and potential utility. However, some limitations and contradictions should also be discussed. The aim of this paper is to briefly describe the new findings, give practical examples of the clinical utility of these methods, compare them with invasive techniques, and review the literature on this subject.

scholarly journals New Aspects in the Diagnosis and Therapy of Fetal Hypoplastic Left Heart Syndrome

2019 ◽  
Vol 79 (08) ◽  
pp. 863-872 ◽  
Author(s):  
Oliver Graupner ◽  
Christian Enzensberger ◽  
Roland Axt-Fliedner
Keyword(s):  
Hypoplastic Left Heart Syndrome ◽  
Treatment Options ◽  
Left Heart ◽  
Hypoplastic Left Heart ◽  
Non Invasive ◽  
New Findings ◽  
Parental Counseling ◽  
The Right ◽  
Systemic Ventricle ◽  
Left Heart Syndrome

AbstractFetal hypoplastic left heart syndrome (HLHS) is a severe congenital heart disease with a lethal prognosis without postnatal therapeutic intervention or surgery. The aim of this article is to give a brief overview of new findings in the field of prenatal diagnosis and the therapy of HLHS. As cardiac output in HLHS children depends on the right ventricle (RV), prenatal assessment of fetal RV function is of interest to predict poor functional RV status before the RV becomes the systemic ventricle. Prenatal cardiac interventions such as fetal aortic valvuloplasty and non-invasive procedures such as maternal hyperoxygenation seem to be promising treatment options but will need to be evaluated with regard to long-term outcomes. Novel approaches such as stem cell therapy or neuroprotection provide important clues about the complexity of the disease. New aspects in diagnostics and therapy of HLHS show the potential of a targeted prenatal treatment planning. This could be used to optimize parental counseling as well as pre- and postnatal management of affected children.

scholarly journals Non-invasive assessment of in-vitro embryo quality to improve transfer success

2015 ◽  
Vol 31 (5) ◽  
pp. 585-592 ◽  
Author(s):  
Tina Rødgaard ◽  
Peter M.H. Heegaard ◽  
Henrik Callesen
Keyword(s):  
Embryo Quality ◽  
Non Invasive ◽  
Transfer Success
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