Evaluation of the Bacteriological risk of solid Medical and Pharmaceutical waste from a Moroccan Hospital Center

2021 ◽  
pp. 2983-2988
Author(s):  
Oussama Chauiyakh ◽  
Aziz Et-tahir ◽  
Kamal Kettani ◽  
Abdelaziz Chaouch ◽  
Halima Bessi
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Pathogenic Bacteria ◽  
Hospital Waste ◽  
Pharmaceutical Waste ◽  
Before And After ◽  
Biology Laboratory ◽  
Ground Material ◽  
Hospital Center

Medical and pharmaceutical waste (MPW) pose a big problem in society and at the healthcare centers because they cause a bacteriological risk in the living being and the environment. Morocco, since 1991, has developed a legal arsenal on medical and pharmaceutical waste. In order to identify pathogens at the level of hospital waste, and to assess their treatment system, this study was carried out at a Moroccan hospital center. The analysis of the samples was performed at the medical biology laboratory of the same hospital. Eleven samples were done at four care units, including two at the internal storage room before treating waste with an ECODAX T300 type mill, and two after treatment from the ground material and leachate. Before crushing 63.6% (7/11) of the samples gave positive cultures. The presence of pathogenic microorganisms such as (Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas stuzerie and Pseudomonas fluorescens) have been identified and their sensitivity to antibiotics has also been determined. After the grinding cycle, the microbiological characterization of the samples on ground material and leachate revealed 100% of bacterial presence, the microorganisms (Escherichia coli and Klebsiella pneumoniae) were identified. The results show that hospital waste encloses multi-resistant pathogenic bacteria before and after their treatment, which raises a big question mark on the quality and effectiveness of the treatment of MPW.

scholarly journals Characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates from the community in Morocco

2011 ◽  
Vol 60 (9) ◽  
pp. 1344-1352 ◽  
Author(s):  
Abouddihaj Barguigua ◽  
Fatima El Otmani ◽  
Mustapha Talmi ◽  
Fatna Bourjilat ◽  
Fatima Haouzane ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Extended Spectrum

scholarly journals UTILIZAÇÃO DE GÁS OZÔNIO NA DESINFECÇÃO DE RESÍDUOS DE SERVIÇOS DE SAÚDE

2018 ◽  
Vol 7 (2) ◽  
pp. 125-139
Author(s):  
Thais Nogueira Gonzaga ◽  
Dora Inés Kozusny-Andreani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Health Care Services ◽  
Pathogenic Bacteria ◽  
Ozone Treatment ◽  
Microbiological Analysis ◽  
Before And After ◽  
Staphylococcus Spp

Nesta pesquisa objetivou-se avaliar a viabilidade técnica da aplicação de ozônio como bactericida e fungicida em amostras de resíduos de serviços de saúde potencialmente infectantes. Foram determinados os     micro-organismos presentes nos resíduos gerados em um hospital particular. Para realização das análises microbiológicas e o tratamento com ozônio o material foi particulado e homogeneizado. As análises microbiológicas foram realizadas antes e após a ozonização.Para os testes de desinfecção foram retirados 10,0g de amostra que foi submetida à ozonização por 5, 10, 15, 20 e 25 minutos com doses de 140,0; 280,0; 420,0; 560,0 e 700,0mg L-1 de ozônio, respectivamente. Verificou-se presença de mesófilos totais, coliformes totais e termotolerantes, Escherichia coli, Pseudomonas aeruginosa, Proteus spp., Staphylococcus aureus, Staphylococcus spp, Candida albicans e Rhizopus spp. O ozônio foi eficiente para eliminação de todos os micro-organismos em 20 minutos; nos primeiros cinco minutos de exposição ao gás verificou-se redução superior a 98%.Palavras-chave: Bactérias patogênicas. Fungos. Ozonização. USING OZONE GAS FOR DISINFECTION OF SOLID WASTE FROM HEALTH CARE SERVICES ABSTRACT: The aim of this research was to evaluate the technical viability of the application of ozone as bactericide and fungicide in samples of potentially infectious health services residues. The microorganisms present in the waste generated in a private hospital were determined. The material was particulated and homogenized to perform the microbiological analysis and to undergo ozone treatment. Microbiological analysis was performed before and after ozonization. For the disinfection tests, 10.0g of sample were removed and submitted to ozonization for 5, 10, 15, 20 and 25 minutes with 140,0; 280,0; 420,0; 560,0 and 700,0mg doses of L-1 of ozone, respectively. It was verified the presence of total mesophiles, total and thermotolerant coliforms, Escherichia coli, Pseudomonas aeruginosa, Proteus spp., Staphylococcus aureus, Staphylococcus spp, Candida albicans and Rhizopus spp. Ozone was efficient while eliminating all microorganisms in 20 minutes; in the first five minutes of gas exposure, the reduction was greater than 98%.Keywords: Pathogenic bacteria. Fungi. Ozonization.

Characterization of Multidrug-Resistant Biofilm-Producing Escherichia coli and Klebsiella pneumoniae in Healthy Cattle and Cattle with Diarrhea

2021 ◽  
Author(s):  
Samiran Bandyopadhyay ◽  
Debaraj Bhattacharyya ◽  
Indranil Samanta ◽  
Jaydeep Banerjee ◽  
Md Habib ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Multidrug Resistant ◽  
Healthy Cattle

Transferable cefoxitin resistance in enterobacteria from Greek hospitals and characterization of a plasmid-mediated group 1 beta-lactamase (LAT-2).

1996 ◽  
Vol 40 (7) ◽  
pp. 1736-1740 ◽  
Author(s):  
M Gazouli ◽  
L S Tzouvelekis ◽  
E Prinarakis ◽  
V Miriagou ◽  
E Tzelepi
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Klebsiella Pneumoniae ◽  
Enterobacter Aerogenes ◽  
Citrobacter Freundii ◽  
Beta Lactamase ◽  
Beta Lactamases ◽  
Greek Hospitals ◽  
Group 1

Cefoxitin resistance in Klebsiella pneumoniae from Escherichia coli strains isolated in Greek hospitals was found to be due to the acquisition of similar plasmids coding for group 1 beta-lactamases. The plasmids were not self-transferable but were mobilized by conjugative plasmids. These elements have also been spread to Enterobacter aerogenes. The most common enzyme was a Citrobacter freundii-derived cephalosporinase (LAT-2) which differed from LAT-1 by three amino acids.

scholarly journals Emergence of CTX-M-15-Producing Enterobacteria in Cameroon and Characterization of a blaCTX-M-15-Carrying Element

2005 ◽  
Vol 49 (1) ◽  
pp. 441-443 ◽  
Author(s):  
J. Gangoue-Pieboji ◽  
V. Miriagou ◽  
S. Vourli ◽  
E. Tzelepi ◽  
P. Ngassam ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Target Site ◽  
Target Site Duplication ◽  
Truncated Form

ABSTRACT CTX-M-15-producing Klebsiella pneumoniae and Escherichia coli emerged recently in Cameroon. CTX-M-15 was encoded by two different multiresistance plasmids, of which one carried an ISEcp1-bla CTX-M-15 element flanked by a 5-bp target site duplication and inserted within a Tn2-derived sequence. A truncated form of this element in the second plasmid was identified.

CHARACTERIZATION OF KLEBSIELLA PNEUMONIAE AND ESCHERICHIA COLI STRAINS THAT PRODUCE CTX-M-2-TYPE BROAD SPECTRUM BETA-LACTAMASE ISOLATED FROM A CHILD WITH LEUKEMIA

2002 ◽  
Vol 21 (3) ◽  
pp. 260-262 ◽  
Author(s):  
Toshiya Ohkawa ◽  
Masao Yoshinaga ◽  
Naoaki Ikarimoto ◽  
Hiroaki Miyanohara ◽  
Koichiro Miyata ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Broad Spectrum ◽  
Beta Lactamase
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