GC-MS Identification of Anti-inflammatory and Anticancer Metabolites in Edible Milky White Mushroom (Calocybe indica) against Human Breast Cancer (MCF-7) Cells

2021 ◽  
pp. 4300-4306
Author(s):  
Mohanasundaram S. ◽  
Rangarajan N. ◽  
Sampath V. ◽  
Porkodi K. ◽  
M.V. Dass Prakash ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Protein Denaturation ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Inhibition Assay ◽  
Human Breast ◽  
Anti Inflammatory ◽  
Calocybe Indica ◽  
Mcf 7

Milky White Mushroom is a species of edible mushroom native to India. The Purpose of this study was to determine the anti-inflammatory and anticancer compounds from Calocybe indica. The ethanolic extract from Calocybe indica were prepared. GC-MS was performed and the major bioactive compounds such as polysaccharides, amino acids, sterols, phytol, and squalene were determined. Calocybe indica was analyzed for their antioxidant activity through In vitro assays such as Free radical activity on DPPH, Hydroxyl radical scavenging assay, Nitric Oxide scavenging assay, Superoxide scavenging assay. Anti-inflammatory effect was estimated by protein denaturation inhibition assay, Proteinase inhibition assay, Cyclooxygenase inhibition assay and Lipoxygenase inhibition assay. Anticancer activity of Calocybe indica extract showed maximum inhibition of 69.11% of growth of human breast cancer cell (MCF 7) at 100μg/ml exposure for about 72 hours. At the end of this study, it was indicated that ethanolic extract of Calocybe indica can be used as an anti- inflammatory and anticancer agent.

In vitro anti-inflammatory and anti-proliferative potential of roots extract of Euphorbia hirta Linn

2021 ◽  
Vol 10 (1) ◽  
pp. 7-9
Author(s):  
Rashmi ◽  
◽  
Pankaj Gupta ◽  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Human Breast Cancer ◽  
Protein Denaturation ◽  
Alcoholic Extract ◽  
Human Breast ◽  
Euphorbia Hirta ◽  
Proliferative Effect ◽  
Anti Inflammatory ◽  
Mcf 7

Aim: The main aim of this current study was to characterize the anti-inflammatory and anti-proliferative activities of alcoholic extract of the roots of euphorbia hirta. Genus Euphorbia contains many therapeutic plants which are widely studied. This study is designed to explore the effects of alcoholic roots extract of euphorbia hirta, on selected cell lines of Human Breast cancer (MCF - 7). Methods: The Human Breast Cancer cell lines i.e. MCF – 7 cell lines were treated with alcoholic roots extract of euphorbia hirta at different concentrations. Anti-proliferative effect of the alcoholic extract was evaluated by assessing the cell viability. MTT assay was used to determine the cell viability at the wavelength of 570 nm. Antiinflammatory potential of extract was evaluated by calculating the percentage inhibition of protein denaturation. Results: The results showed the significant anti-inflammatory potential as it effectively inhibits the protein denaturation. The alcoholic roots extract of euphorbia hirta also showed moderate cytotoxic effect against MCF - 7 cell-lines (P ≤0.05). Conclusions: The results showed that this extract had significant anti-inflammatory potential and moderate anti-proliferative effect, as extract was able to inhibit the proliferation of tumor cells. Hence, it seems to be a virtuous contender to protect against growth of tumor cells.

scholarly journals Anti-Inflammatory Effects of a Methanol Extract from the Marine SpongeGeodia cydoniumon the Human Breast Cancer MCF-7 Cell Line

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Susan Costantini ◽  
Giovanna Romano ◽  
Fabiola Rusolo ◽  
Francesca Capone ◽  
Eliana Guerriero ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Methanol Extract ◽  
Marine Sponges ◽  
Biologically Active ◽  
Dependent Manner ◽  
Human Breast ◽  
Normal Human Breast ◽  
Anti Inflammatory ◽  
Mcf 7

Many research groups are working to find new possible anti-inflammatory molecules, and marine sponges represent a rich source of biologically active compounds with pharmacological applications. In the present study, we tested different concentrations of the methanol extract from the marine sponge,Geodia cydonium, on normal human breast epithelial cells (MCF-10A) and human breast cancer cells (MCF-7). Our results show that this extract has no cytotoxic effects on both cell lines whereas it induces a decrease in levels of VEGF and five proinflammatory cytokines (CCL2, CXCL8, CXCL10, IFN-γ, and TNF-α) only in MCF-7 cells in a dose-dependent manner, thereby indicating an anti-inflammatory effect. Moreover, interactomic analysis suggests that all six cytokines are involved in a network and are connected with some HUB nodes such as NF-kB subunits and ESR1 (estrogen receptor 1). We also report a decrease in the expression of two NFKB1 and c-Rel subunits by RT-qPCR experiments only in MCF-7 cells after extract treatment, confirming NF-kB inactivation. These data highlight the potential ofG. cydoniumfor future drug discovery against major diseases, such as breast cancer.

scholarly journals Impact of ethanolic extract of Mikania glomerata on human breast cancer (MCF 7) cell line

2016 ◽  
Vol 2 (4) ◽  
pp. 94 ◽  
Author(s):  
Sarojini S. ◽  
Senthilkumaar P. ◽  
Ramesh V.
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Ethanolic Extract ◽  
Breast Cancer Cell Lines ◽  
Human Breast ◽  
Anti Cancer ◽  
Mikania Glomerata ◽  
Mcf 7

The ethanol extract of Mikania glomerata has anti-proliferative effect on the human breast cancer cell lines. The object of the present work is to investigate the anti-cancer effect of Mikania glomerata ethanolic extract on breast cancer. Soxlet fractions using crude ethanolic extract of Mikania glomerata was prepared by standard extraction protocols. To check the antiproliferative effect of this extract, the extract chosen was tested for cell viability on the breast cancer cells MCF 7 in different concentrations. Cell viability was evaluated by MTT assay for 24 hour and 48 hours. The LD50 value was calculated and different morphometric assays were performed with the effective dose of the extract. The effect of the extract on the normal cell was evaluated as well. Cell proliferation, cell cycle, Clonogenic survival, Apoptosis and MTT assays were performed. The ethanolic extract showed a dose-dependent and time dependent inhibition on cell proliferation in the breast cancer cell lines. It showed low cytotoxicity in the normal cells and inhibited cellular adhesion and wound healing in treated cancer cells. The present study suggests that the leaf extract from Mikania glomerata induces anticancer effect on the breast cancer cells. Further study might help to confirm it as an anti-cancer drug.

scholarly journals Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Verisa Chowjarean ◽  
Kamala Sadabpod
Keyword(s):  
Breast Cancer ◽  
Colony Formation ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Ethanolic Extract ◽  
Bioactive Compound ◽  
The Novel ◽  
Human Breast ◽  
Phosphorylated Akt ◽  
Mcf 7

Background/Aim. Grammatophyllum speciosum Blume exhibits various promising pharmacological activities. However, its effect on breast cancer has not been determined. Materials and Methods. The antiproliferation effects of the G. speciosum pseudobulb ethanolic extract (GSE) and isovitexin (bioactive constituent) were investigated on the MCF-7 human breast cancer cell line using MTT and colony formation assay. The expression levels of proliferation-regulatory proteins were determined by western blotting. Results. Noncytotoxic concentrations of GSE significantly suppressed the proliferation of MCF-7 cells. Tumor colony formation decreased in both number and size. The level of phosphorylated AKT and β-catenin was suppressed by GSE treatment. Antiproliferation was observed in isovitexin-treated MCF-7 cells in the form of inhibited colony formation and reduced expression of phosphorylated AKT and β-catenin protein. Conclusions. This study demonstrates the novel effect of G. speciosum as an antiproliferative via suppression of the AKT/β-catenin-dependent pathway. This may prompt further investigation of this plant in breast cancer therapy.

scholarly journals Effect of ethanolic extract of Reissantia indica on the human breast cancer cell line (MCF-7)

2018 ◽  
Vol 7 (1) ◽  
pp. 10-12
Author(s):  
Gayathri P ◽  
◽  
Dhana Sekar V ◽  
Sudhakar K ◽  
Sangeetha M ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Plant Extract ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Ethanolic Extract ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Plant Parts ◽  
Mcf 7

Reissantia indica belongs to the family Celastraceae, is a native shrub to Maharashtra and Bengal, India. Its distinct plant parts are said to possess different activities, natives of Maharashtra and Bengal are using the plant parts for various purposes. In this experiment, the effect of ethanolic extract to destroy the cancer cells was studied. Study was carried on with human breast cancer cell line (MCF-7). Preparation of cell line was done using the reagents under standard protocol. Effect of plant extract on the cancer cells were recorded at two different timings (24 & 48 Hrs) respectively. Various doses of plant extract and standard were taken and introduced into cell line. Using 96 well plates the cells were seeded and studied for the viability. From the tabulated records, it clearly shows us the dose dependent response for the inhibition of cells. The maximum concentration of the plant extract 100µg/ml was found to be effective

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