scholarly journals The Effect of Water Instant Noodle on Histologycal Appearance of Pregnant Mice (Mus musculus L.)

2021 ◽  
Vol 1 (1) ◽  
pp. 39-49
Author(s):  
Sister Sianturi
Keyword(s):  
Statistical Analysis ◽  
Cell Damage ◽  
Pregnant Female ◽  
Staining Procedure ◽  
Histological Structure ◽  
Female Mice ◽  
Pregnant Mice ◽  
Hepatocyte Cells ◽  
Boiled Water ◽  
Instant Noodle

ABSTRAK   Penelitian ini dilakukan sebagai lanjutan dari penelitian sebelumnya yaitu uji pemberian air rebusan mie instan pada mencit betina bunting dan diperoleh hasil bahwa terdapat kelainan fetus tetapi pada analisis statistic kelainan tersebut tidak signifikan. Penelitian ini dilakukan untuk mengetahui gambaran histologi organ hepar mencit betina bunting setelah pemberian air rebusan mie instan. Tahapan penelitian ini adalah sebagai berikut: persiapan hewan uji mencit betina bunting yang sudah diberi perlakuan air rebusan mie instan dari 3 merek yang berbeda yaitu P1, P2, dan P3.Masing-masing perlakuan tersebut terdiri atas pemberian air rebusan dengan konsentrasi yang berbeda yaitu 30%, 50%, dan 70% selama 14 hari, kemudian mencit dikorbankan lalu diambil organ hepar,  pembuatan preparat histology, lalu diamati struktur histologinya. Metode pembuatan preparat histologi mengikuti prosedur pewarnaan HE (Haematoxyllin-Eosine). Hasil penelitian menunjukkan adanya perubahan struktur sel hepatosit hepar pada perlakuan yaitu degenerasi midzonal dan nekrosis. Hal ini menunjukkan adanya pengaruh pemberian perlakuan air rebusan mi instan terhadap susunan sel hepar. Penelitian menggunakan jumlah sampel organ yang terbatas sehingga perlu dilakukan penelitian dengan sampel yang lebih banyak dan diperlukan adanya analisis statistik untuk mengetahui tingkat derajat kerusakan sel.   ABSTRACT This study was conducted based on previous research, to test of giving instant noodle boiled water to pregnant female mice and the results showed that there were fetal abnormalities but the statistical analysis of these abnormalities was not significant. This study was conducted to determine the histology of the liver of pregnant female mice after giving instant noodle boiled water. The stages of this research are as follows: preparation of pregnant female mice that have been treated with instant noodle boiled water from 3 different brands, namely P1, P2, and P3. Each treatment consisted of giving boiled water with different concentrations of 30 %, 50%, and 70% for 14 days, then the mice were sacrificed and then the liver was taken, histology preparations were made, then the histological structure was observed. The method of making histology preparations followed the HE (Haematoxyllin-Eosine) staining procedure. The results showed that there were changes in the structure of the liver hepatocyte cells in the treatment, namely midzonal degeneration and necrosis. This shows that there is an effect of giving instant noodle boiled water treatment on the composition of liver cells. The study used a limited number of organ samples, so it is necessary to do research with more samples and statistical analysis is needed to determine the degree of cell damage

scholarly journals The Effect of Folic Acid as Supportive Therapy of Spiramycine on Weight of Foetus to Toxoplasma gondii – Infected Pregnant Mice (Mus musculus)

2019 ◽  
Vol 3 (1) ◽  
pp. 33
Author(s):  
Alfina Azkiana ◽  
Boedi Setiawan ◽  
Erma Safitri ◽  
Lucia Tri Suwanti ◽  
Mufasirin Mufasirin ◽  
...  
Keyword(s):  
Folic Acid ◽  
Toxoplasma Gondii ◽  
Mus Musculus ◽  
Supportive Therapy ◽  
Pregnant Female ◽  
Anova Test ◽  
Analytical Balance ◽  
Female Mice ◽  
Pregnant Mice ◽  
T1 And T2

This research aimed to investigate the effect of folic acid as supportive therapy of spiramycine on weight of foetus to Toxoplasma gondii-infected pregnant mice (Mus musculus). Twenty pregnant female mice were divided into four groups as C -, C +, T1 and T2. C +, T1 and T2 were infected by Toxoplasma gondii. C – and C + administered orally 0.5 ml aquadest, T1 administered orally 130 mg/kg BW spiramycine and 0.052 µg/g BW folic acid and T2 administered orally 0.052 µg/g BW folic acid. Experimental groups received the treatments for 5 days, then animals of each groups were sacrified. Foetuses were dissected out for observation. The weight of fetuses were measured using an analytical balance. The data weight of foetuses was presented descriptively and analyzed by ANOVA test and continued by Tukey HSD. From this study, the weight of foetuses from the pregnant mice of T1 and T2 have difference compared with the controls. The result of this research is folic acid affects the weight of foetuses to Toxoplasma gondii-infected pregnant mice.

PRODUCTION OF CONGENITAL DEFECTS IN THE OFFSPRING OF PREGNANT MICE TREATED WITH CORTISONE

PEDIATRICS ◽  
1951 ◽  
Vol 8 (4) ◽  
pp. 527-533
Author(s):  
F. CLARKE FRASER ◽  
T. D. FAINSTAT
Keyword(s):  
Cleft Palate ◽  
Congenital Malformations ◽  
Pregnant Female ◽  
Congenital Defects ◽  
Genetic Constitution ◽  
Female Mice ◽  
Pregnant Mice ◽  
Gestational Stage

It has been shown that cortisone, injected into pregnant female mice, will produce cleft palate and other congenital malformations in the resulting young. The incidence of cleft palate varies according to the genetic constitution of the treated mice, the dosage of cortisone used and the gestational stage at which treatment is begun.

Statistical Analysis of Measures of Arousal in Ovariectomized Female Mice

2001 ◽  
Vol 39 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Jonathan Frohlich ◽  
Maria Morgan ◽  
Sonoko Ogawa ◽  
Leslie Burton ◽  
Donald Pfaff
Keyword(s):  
Statistical Analysis ◽  
Female Mice

scholarly journals Co-factor Influences the Severity of Zika Virus-Associated Microcephaly

2021 ◽  
Author(s):  
Adriano Barreto Nogueira ◽  
Breno Bonadies Andrade ◽  
Leonardo Yuri Kasputis Zanini ◽  
Hillary Sayuri Ramires Hoshino ◽  
Natalia Camargo Ortega ◽  
...  
Keyword(s):  
Experimental Model ◽  
Intravenous Injection ◽  
Zika Virus ◽  
Brain Lesion ◽  
Methylation Pattern ◽  
Mtor Signaling ◽  
Pregnant Female ◽  
Deficient Diet ◽  
Female Mice ◽  
Nutritional Deficits

Microcephaly has been regarded the most remarkable consequence of the Zika virus (ZIKV) epidemic in Brazil 2015. It remains to be determined whether there are factors that contribute to the degree of brain lesion associated with ZIKV infection during pregnancy. Previous studies showed that socioeconomic conditions correlate with ZIKV-associated microcephaly. Certain nutritional deficits display the potential to interfere in the mechanistic target of rapamycin (mTOR) signaling, which plays a major role in the pathophysiology of ZIKV-associated microcephaly. We hypothesize that a nutritional or environmental co-factor that interferes in mTOR signaling correlates with ZIKV-associated birth defects. To assess this hypothesis, we plan to: 1) develop a mouse model of ZIKV-associated microcephaly through intravenous injection of ZIKV and rapamycin for a straightforward interference on mTOR receptor; 2) determine in the experimental model and in cases of ZIKV-associated microcephaly the epigenetic signature (DNA methylation pattern) in neurons and muscle cells harvested by biopsy, and in hematopoietic and mesenchymal stem cells sorted from blood; 3) analyze through mass spectrometry in serum of pregnant female mice submitted to ZIKV and rapamycin injection and in serum of mothers of children with ZIKV-associated microcephaly the metabolomic pattern of cholesterol (a nutritional status marker), vitamin A and its metabolite retinoic acid, folate, and other metabolites related to these three nutritional factors; 4) check whether pregnant female mice submitted to intravenous injection of ZIKV and feed with a deficient diet of the most likely co-factor found in this study give birth to microcephalic mice with features that mimic clinical cases. In summary, our general objective is to develop an experimental model that mimics ZIKV-associated microcephaly cases and to find a co-factor involved in the microcephaly outbreak in Brazil 2015.

Schistosoma japonicum infection in pregnant mice

1999 ◽  
Vol 73 (3) ◽  
pp. 277-278 ◽  
Author(s):  
M. Bendixen ◽  
M.V. Johansen ◽  
J. Andreassen ◽  
P. Nansen
Keyword(s):  
Schistosoma Japonicum ◽  
Post Infection ◽  
Congenital Infection ◽  
Pregnant Female ◽  
Control Groups ◽  
Nmri Mice ◽  
Pregnant Mice

Ten 1-week and ten 2-weeks pregnant female NMRI mice were experimentally exposed to 70 Schistosoma japonicum cercariae. Ten littermice from each group were examined for worms by perfusion 4, 6 and 8 weeks post infection. Although the mothers (n = 15) were found infected with 15.5 ± 13.4 worms at perfusion 6 and 7 weeks post infection, no worms were found in any of the examined littermice, as well as no detection of faecal or tissue eggs. Litter sizes did not differ from control groups and all littermice were healthy. The present study therefore suggests that congenital infection with S. japonicum does not occur in percutaneously infected mice and that infection of the mother during pregnancy does not seem to affect the offspring.

104 SURVIVAL AND APOPTOSIS RATES AFTER VITRIFICATION OF EARLY, EXPANDED, AND HATCHED CALF AND COW BLASTOCYSTS IN VITRO-PRODUCED USING CRYOTOP AS A DEVICE

2010 ◽  
Vol 22 (1) ◽  
pp. 211
Author(s):  
R. Morató ◽  
D. Izquierdo ◽  
M. T. Paramio ◽  
T. Mogas
Keyword(s):  
Cell Damage ◽  
Survival Rates ◽  
Developmental Competence ◽  
Staining Procedure ◽  
Tunel Staining ◽  
Dna Integrity ◽  
Blastocyst Development ◽  
Integrity Index ◽  
High Degree

Two experiments were designed to determine the ability of 7 and 8 day in vitro-produced blastocysts to survive to the vitrification procedure. Embryos were classified as early blastocysts, expanded, or hatching/hatched blastocysts. Vitrification was done using cryotop devices as described Du et al. (2007). After warming, blastocysts were incubated for 3 h in SOF medium. In the first experiment, we examined the developmental competence of early blastocysts, expanded blastocysts, and hatching/hatched blastocysts after vitrification using the Cryotop method. In the second experiment, warmed blastocysts that had been vitrified on cryotops were fixed in 4% formaldehyde and incubated with TUNEL staining for detecting DNA damaged nuclei. The percentage of TUNEL positive and negative blastomeres was assessed by confocal microscopy. In all experiments cow and calf blastocysts were compared. When the results according to the developmental stage were analyzed, no differences in the survival rates after vitrification of expanded and hatched blastocysts were observed at Day 8 from cow and calf blastocysts. After warming, survival rates of 52.4 and 50% were noted in the groups of expanded and hatched blastocysts respectively from Day 8 cow blastocysts. Similar results were observed in the groups of expanded (54.5%) and hatched (59.4%) blastocysts from Day 8 calf blastocysts. When embryos were vitrified at Day 7, survival rates of 78.4 and 66.7% were observed after warming expanded and hatched blastocysts from cows. In calves, a significant increase in the survival up to 83.3 and 80% was observed after warming expanded and hatched blastocysts. The lowest survival rates were observed in early blastocysts (from 26 to 51%), particularly in those vitrified at Day 8 (≤40%). Following vitrification, cell death was monitored in blastocysts 3 h after warming by TUNEL labelling of cells with damaged DNA. The TUNEL staining procedure was undertaken on Day 7 calf (n = 23) and cow (n = 25) blastocysts, as well as on Day 8 calf (n = 22) and cow (n = 30) blastocysts. When taking into account the stage of blastocyst development, there was a trend toward higher DNA integrity index after warming of expanded and hatched blastocysts compared with early blastocysts in calf and cow groups. So, cell damage was minimal in those blastocysts vitrified at expanded and hatched stage and rates were comparable with those from control fresh blastocysts. These findings suggest that the Cryotop technique seems to be particularly useful for blastocysts presenting a high degree of expansion (expanded and hatched blastocysts), mainly those blastocysts vitrified and warmed at Day 7.

The Relationship Between Pregnancy Block and Infanticide in House Mice (Mus Musculus Domesticus) During Lactational Pregnancy

Behaviour ◽  
1996 ◽  
Vol 133 (13-14) ◽  
pp. 1023-1050 ◽  
Author(s):  
Sarah Lenington ◽  
Carol B. Coopersmith
Keyword(s):  
Mus Musculus ◽  
Sexual Contact ◽  
Pregnant Female ◽  
Delivery Rate ◽  
House Mice ◽  
Mus Musculus Domesticus ◽  
Female Mice ◽  
Postpartum Estrus ◽  
The Relationship

AbstractRecently mated female house mice separated from their mates and exposed to unfamiliar (strange) males often undergo pregnancy disruption. A favored explanation for this phenomenon, known as pregnancy block, is that it has evolved as a female counterresponse to potential infanticide by males. Curiously, pregnancy block is believed not to occur during lactational pregnancy, even though that lactational pregnancy is thought to be common in house mice. We examined the potential for male-induced pregnancy block to occur during lactational pregnancy in female mice after lactation disruption by both male infanticide (Expt 1) and litter removal (Expt 2). Strange males were paired with females that had both recently given birth to a first litter and potentially been reimpregnanted by the original stud male during postpartum estrus. Hence, some of the females' second litters were sired by the stud males and pregnancy block could then be detected as a drop in the delivery rate of such litters among these experimental females relative to control females that were not exposed to strange males. Pairs remained housed together until after a second litter was born. Thus, we were also able to measure the influence of prolonged heterosexual cohabitation on infanticide by strange males towards subsequent litters. If, as some have found, non-sexual contact with a pregnant female suppresses male infanticide, then the importance of male infanticide as an explanation for pregnancy block is further drawn into question. When strange males were introduced to lactating dams approximately one and a half days postpartum (Expt 1), male infanticide effectively halted lactation. However, none of these females subsequently showed pregnancy block. In contrast, when strange males were introduced to dams approximately one day postpartum and lactation was interrupted even earlier (Expt 2), pregnancy block did occur. There was no evidence for a cohabitation-induced reduction of male infanticide in either experiment; most strange males initially categorized as infanticidal subsequently killed females' second litters, provided they had not sired those litters. Despite the lack of cohabitation effects on male infanticide, because pregnancy block may only occur under restricted conditions during lactational pregnancy, the role of male infanticide in shaping the evolution of pregnancy block may be less straightforward than previously thought.

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