Cytokine Gene Expression in the Tunica Albuginea of Patients with Peyronie's Disease. Pilot Study with a Control Group

Calf disbudding is a painful husbandry practice on dairy and beef cattle farms. An objective measurement of pain is useful to reliably evaluate the pain intensity and anti-nociceptive (analgesic) efficacy of therapeutic agents. The aim of this study was to investigate the changes in peripheral leucocyte inflammatory cytokine gene expression in calves after disbudding, and to assess whether the changes in cytokine gene expression could be an indicator of the efficacy of analgesic drugs. In a randomised controlled study, 16 calves (aged 31 to 41 days and weighing 58 to 73 kg), undergoing routine disbudding, were randomly allocated into two groups (n = 8 in each group). Calves in the control group received no analgesic, while those in the treatment group received 0.5 mg kg−1 meloxicam subcutaneously prior to disbudding. Disbudding was performed using an electric debudder. Blood (10 mL) was sampled from the jugular vein just before and 4 and 24 h post-disbudding, RNA was extracted from leukocytes, and the transcription of 12 genes of interest was assessed using nCounter gene expression assay. The results showed significantly higher transcription (compared to baseline values) of the studied genes (except CRH, IFNγ, and IL10) in the control group calves at either 4 or 24 h post-disbudding. The administration of meloxicam one hour before disbudding significantly attenuated the upregulation of IL6, PGHS2, TAC1, NOS1, and CRH gene transcription post-disbudding, while it did not suppress the elevated transcription of acute and pro-inflammatory cytokines such as IL1β, IFNγ, IL8, and TNFα genes. In conclusion, nCounter gene expression assay seems to be a promising tool to study the expression of cytokine genes and thus could be used for the pre-clinical evaluation of novel analgesics.

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Immunomodulation mediated by a herbal syrup containing a standardized Echinacea root extract: A pilot study in healthy human subjects on cytokine gene expression

Phytomedicine ◽

10.1016/j.phymed.2014.04.034 ◽

2014 ◽

Vol 21 (11) ◽

pp. 1406-1410 ◽

Cited By ~ 16

Author(s):

B. Dapas ◽

S. Dall’Acqua ◽

R. Bulla ◽

C. Agostinis ◽

B. Perissutti ◽

...

Keyword(s):

Gene Expression ◽

Pilot Study ◽

Human Subjects ◽

Cytokine Gene Expression ◽

Root Extract ◽

Cytokine Gene ◽

Healthy Human ◽

Healthy Human Subjects

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RNA-sequencing profiling analysis of pericyte-derived extracellular vesicle–mimetic nanovesicles-regulated genes in primary cultured fibroblasts from normal and Peyronie’s disease penile tunica albuginea

BMC Urology ◽

10.1186/s12894-021-00872-x ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Guo Nan Yin ◽

Shuguang Piao ◽

Zhiyong Liu ◽

Lei Wang ◽

Jiyeon Ock ◽

...

Keyword(s):

Gene Expression ◽

Rna Sequencing ◽

Tunica Albuginea ◽

Global Gene Expression ◽

Extracellular Vesicle ◽

Peyronie’S Disease ◽

Rt Pcr ◽

Cultured Fibroblasts ◽

Primary Fibroblasts ◽

Peyronie's Disease

Abstract Background Peyronie’s disease (PD) is a severe fibrotic disease of the tunica albuginea that causes penis curvature and leads to penile pain, deformity, and erectile dysfunction. The role of pericytes in the pathogenesis of fibrosis has recently been determined. Extracellular vesicle (EV)–mimetic nanovesicles (NVs) have attracted attention regarding intercellular communication between cells in the field of fibrosis. However, the global gene expression of pericyte-derived EV–mimetic NVs (PC–NVs) in regulating fibrosis remains unknown. Here, we used RNA-sequencing technology to investigate the potential target genes regulated by PC–NVs in primary fibroblasts derived from human PD plaque. Methods Human primary fibroblasts derived from normal and PD patients was cultured and treated with cavernosum pericytes isolated extracellular vesicle (EV)–mimetic nanovesicles (NVs). A global gene expression RNA-sequencing assay was performed on normal fibroblasts, PD fibroblasts, and PD fibroblasts treated with PC–NVs. Reverse transcription polymerase chain reaction (RT-PCR) was used for sequencing data validation. Results A total of 4135 genes showed significantly differential expression in the normal fibroblasts, PD fibroblasts, and PD fibroblasts treated with PC–NVs. However, only 91 contra-regulated genes were detected among the three libraries. Furthermore, 20 contra-regulated genes were selected and 11 showed consistent changes in the RNA-sequencing assay, which were validated by RT-PCR. Conclusion The gene expression profiling results suggested that these validated genes may be good targets for understanding potential mechanisms and conducting molecular studies into PD.

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Increasing effectiveness of conservative treatment of Peyronie’s disease

Andrology and Genital Surgery ◽

10.17650/2070-9781-2018-19-4-78-84 ◽

2018 ◽

Vol 19 (4) ◽

pp. 78-84

Author(s):

O. B. Zhukov ◽

A. E. Vasilyev ◽

M. E. Novikov

Keyword(s):

Treatment Group ◽

Conservative Treatment ◽

Erectile Function ◽

Early Stage ◽

Tunica Albuginea ◽

Peyronie’S Disease ◽

Control Group ◽

Plaque Size ◽

Study Objective ◽

Peyronie's Disease

The study objectiveis to investigate the effectiveness of conservative treatment of Peyronie’s disease using antifibrotic agents.Materials and methods. The study included 32 patients aged 44–65 years with diagnosed Peyronie’s disease. The patients were examined using the International Index of Erectile Function, Visual Analog Scale, tunica albuginea of the penis elasticity testing, ultrasound examination (with intracavernous pharmacological testing and dynamic spiral tomography if necessary). The patients were divided into 2 groups. The patients in the treatment group (n = 16) received magnetic laser therapy at the area of the plaque with the “Mustang-urolog” device, as well as vacuum laser treatment with the “Yarovit” device and bovhyaluronidase azoximer as injections and later suppositories. In the comparison group (n = 16), similar therapy was used without bovhyaluronidase azoximer. Control examination was performed 3 months later. If conservative treatment was ineffective, the patients were forwarded to surgical treatment.Results. As a result of conservative treatment, density of the tunica albuginea and cavernous bodies, structure of the cavernous bodies improved; the International Index of Erectile Function increased, and pain intensity decreased. In the treatment group, a stronger tendency toward improvement was observed compared to the control group. Decrease in the plaque size was observed in 63.3 % of the patients in the treatment group and in 43.7 % of the patients in the control group. At the control examination, plaques weren’t detected in 6 and 3 patients, respectively.Conclusion. Conservative treatment is effective in patients with early stage of Peyronie’s disease with moderate curvature of the penis (<30°) and plaque size <2 cm. We recommend including bovhyaluronidase azoximer in the combination treatment regimen of these patients for increased effectiveness.

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Downregulation of miR-29b is associated with Peyronie’s disease

Urologia Journal ◽

10.1177/03915603211036628 ◽

2021 ◽

pp. 039156032110366

Author(s):

Vinicius Genuino dos Santos ◽

Gabriel Arantes dos Santos ◽

Cristóvão Barbosa Neto ◽

Nayara Izabel Viana ◽

Ruan Pimenta ◽

...

Keyword(s):

Messenger Rna ◽

Tunica Albuginea ◽

Corpus Cavernosum ◽

Small Sample ◽

Peyronie’S Disease ◽

Control Group ◽

Fibrous Plaque ◽

Peyronie's Disease ◽

Collagen Genes ◽

First Time

Background: Peyronie’s disease (PD) is characterized by the formation of fibrous plaque in tunica albuginea, causing several problems in patients. The etiology of this disease is not fully understood, and there are few effective treatments. To better understand the molecular pathways of PD, we studied miR-29b, a microRNA that could be involved with this illness. MicroRNAs are endogenous molecules that act by inhibiting messenger RNA. MiR-29b regulates 11 of 20 collagen genes and the TGF-β1 gene, which are related to PD progression. Methods: We compared miR-29b expression in 11 patients with PD and 14 patients without PD (control group). For the patients with PD, we utilized samples from the fibrous plaque ( n = 9), from the tunica albuginea ( n = 11), and from the corpus cavernosum ( n = 8). For the control group, we utilized samples from the tunica albuginea ( n = 14) and from the corpus cavernosum ( n = 10). MiR-29b expression was determined by q-PCR. Results: We found a downregulation of miR-29b in the fibrous plaque, tunica albuginea and corpus cavernosum of patients with PD in comparison with the control group ( p = 0.0484, p = 0.0025, and p = 0.0016, respectively). Conclusion: Although our study has a small sample, we showed for the first time an evidence that the downregulation of miR-29b is associated with PD.

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