A Phytotherapic Approach to Reduce Sperm DNA Fragmentation in Patients with Male Infertility

2016 ◽  
Vol 84 (2) ◽  
pp. 79-82 ◽  
Author(s):  
Marco Capece ◽  
Giuseppe Romeo ◽  
Antonio Ruffo ◽  
Leo Romis ◽  
Salvatore Mordente ◽  
...  
Keyword(s):  
Male Infertility ◽  
Dna Fragmentation ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Secondary Outcome ◽  
Tribulus Terrestris ◽  
Sperm Dna Fragmentation ◽  
Male Factor ◽  
Sperm Analysis

Introduction Infertility affects 50 to 80 million (between 8 and 12% of couples). Male factor is a cause of infertility in almost half of the cases, mainly due to oligoasthenoteratozoospermia. DNA fragmentation is now considered an important factor in the aetiology of male infertility. We studied the effects on semen analysis and on DNA fragmentation of in vivo admnistration of Myo-Inositol and Tribulus Terrestris plus Alga Ecklonia plus Biovis (Tradafertil; Tradapharma Sagl, Swizerland) in men with previously diagnosed male infertility. Materials and Methods Sixty patients were enrolled in the present study and were randomized into two subgroups: the group A who received Myo-inositol 1000 mg, Tribulus Terrestris 300 mg, Alga Ecklonia Bicyclis 200 mg and Biovis one tablet a day for 90 days, and the group B (placebo group) who received one placebo tablet a day for 90 days. The primary efficacy outcome was the improvement of semen characteristics after 3 months’ therapy and the secondary outcome was the reduction of the DNA fragmentation after treatment. Results The groups were homogenous for age, hormonal levels, sperm concentration and all parameters of sperm analysis. Sperm concentration and progressive motility improved after treatment with Tradafertil (3.82 Mil/ml vs. 1.71 Mil/ml; p<0.05; 4.86% vs. 1.00%; p<0.05) as well as the DNA fragmentation (-1.64% vs -0.39%, p<0.001). No side effects were revealed. Conclusions In conclusion, we can affirm that Tradafertil is safe and tolerable. It is a new phytotherapic approach to Oligoasthenoteratospermia (OAT) syndrome that could lead to good results without interacting with hypothalamic–pituitary–gonadal axis.

P–068 CatSper4 cation channel expression is low in male infertility and is affected by cryopreservation

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
N Kilic ◽  
T İrez ◽  
N Dayiolu
Keyword(s):  
Male Infertility ◽  
Dna Fragmentation ◽  
Semen Analysis ◽  
Middle Part ◽  
Cation Channel ◽  
The Other ◽  
Control Group ◽  
Sperm Dna Fragmentation ◽  
Negative Effects ◽  
Sperm Dna

Abstract Study question Is CatSper4 expression in sperm related to functional parameters and does cryopreservation affect CatSper4 expression? Summary answer In this study, it was aimed to investigate whether CatSper4 has a relationship with sperm parameters and is CatSper 4 affected by cryopreservation. What is known already CatSper membrane channels, known as cation channels, are thought to play an important role in the insufficiency of sperm physiology, acrosome reaction, and chemotaxis movement. There is no study on cation channel distribution in an infertile male patient. In addition, studies conducted in recent years have shown that cryopreservation techniques have negative effects on sperm DNA, but there is no analysis in the literature regarding the effects of cryopreservation on CatSper4 ion channel proteins. Study design, size, duration Samples of the patients who applied to the Andrology laboratory in the Medical Park Hospital IVF unit between March 1 and June 1 in 2020 were included in the study. Also, patients with no family history of no genetic anomalies , no varicocele and azoospermia were included.The study were divided into 4 groups in accordance with the male infertility guideline of the European Association of Urology as normozoospermic (control group), the asthenoteratozoospermia, teratozoospermia, and oligoastenotheratozoospermia. Participants/materials, setting, methods In this prospective study, semen analysis, DNA fragmentation, and CatSper 4 by IHC of control group patients with normospermia (n = 40) and oligospermia(n = 50), asthenospermia(n = 40), and teratozoospermia(n = 38) patients were compared and differences resulting from cryopreservation were evaluated by Wilcoxon signed Ranks Test. Main results and the role of chance It was observed that CatSper4 protein positivity was localized in the middle part of the sperm and it was statistically higher in the normozoospermic patient group compared to the other groups (p = 0,01). When the positivity values of CatSper4 protein before and after freezing were compared in the groups, it was seen that the values decreased (p = 0,001,p=0,01). Sperm DNA fragmentation was found to be lowest in normospermia and statistically significantly higher in other groups. Cryopreservation application increased DNA fragmentation in all groups (p &lt; 0,001 , p &lt; 0,01). Limitations, reasons for caution Unfortunately, embryo screening in patients with low CatSper4 expression is not available in the present study. Soon we plan to screen a broader clinical pregnancy series and present the IVF results associated with CatSper4. Wider implications of the findings: Our study indicated that, CatSper4 expression is quite high in normospermia when compared with the other groups, particularly oligoasthenoteratozoospermia and asthenoteratozoospermia. There are almost no studies on this subject in the literature, and we think that it should be studied in larger patient groups and in unexplained infertile cases. Trial registration number Not applicable

scholarly journals Proteomic Analysis in Seminal Plasma of Fertile Donors and Infertile Patients with Sperm DNA Fragmentation

2020 ◽  
Vol 21 (14) ◽  
pp. 5046
Author(s):  
Alba Fernandez-Encinas ◽  
Agustín García-Peiró ◽  
Javier del Rey ◽  
Jordi Ribas-Maynou ◽  
Carlos Abad ◽  
...  
Keyword(s):  
Male Infertility ◽  
Dna Fragmentation ◽  
Seminal Plasma ◽  
Recurrent Miscarriage ◽  
Semen Analysis ◽  
Sperm Chromatin ◽  
Sperm Dna Fragmentation ◽  
Plasma Samples ◽  
Sperm Dna ◽  
Dispersion Test

Seminal plasma proteomics studies could represent a new approach for the determination of molecular elements driving male infertility, resulting in a better male infertility characterization. The aim of this study is to investigate proteomic differences in seminal plasma samples from fertile and infertile individuals. For that, semen samples were selected according to semen analysis, clinical pathology, and values of sperm DNA fragmentation (alkaline and neutral Comet assay and Sperm Chromatin Dispersion test). A total of 24 seminal plasma samples classified in four groups were processed: fertile donors (FD), recurrent miscarriage patients (RM), asthenoteratozoospermic patients (ATZ), and asthenoteratozoospermic patients with varicocele (ATZ-VAR). Results obtained by 2D-differential gel electrophoresis (2D-DIGE) revealed 26 spots significantly increased in fertile donors when compared to patient groups. Also, eight spots in the ATZ group and two in the ATZ-VAR group were decreased compared to the other groups. Twenty-eight proteins were identified by mass spectrometry (MS), most of them involved in metabolic and cellular processes and with a catalytic or binding function. Protein–protein interactions through Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) tool suggest that a large part of them were associated with each other. Furthermore, most of them were associated with ubiquitin C, indicating that it could play an important regulation role, resulting in a potential male infertility biomarker.

scholarly journals Can Utilization of Sperm DNA Fragmentation (SDA) Tests in Combination with Time Lapse Microscopy Help in Improving ICSI Implantation Rates in Unexplained Recurrent Implantation Failures Utilizing Double Stranded SDA as the Causative Factor for the same

2020 ◽  
Vol 4 (1) ◽  
Keyword(s):  
Dna Damage ◽  
Dna Fragmentation ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Time Lapse ◽  
Sperm Dna Fragmentation ◽  
Time Lapse Microscopy ◽  
Sperm Dna ◽  
Implantation Rates ◽  
Embryo Kinetics

Over 50% of intracytoplasmic sperm injection (ICSI) cycles don’t display implantation. Hence laboratories make their maximum efforts to select the best embryos as far as implantation enhancement is concerned. Further utilization of available technologies like time lapse recording have been made in a large number of artificial reproductive technology (ART) centres. Various studies that utilize embryo kinetics have implicated that time when embryo cleavage may prove to be an important factor that determines the implantation potential of an embryo. With this variety of algorithms mathematic wise have been used to forecast which the best embryos are for transfer. But the efficacy of these might be influenced by multiple confounding factors. Thus work on biomarkers that can forecast good ART warrants newer embryo selection basis. Regarding conventional ICSI, typical standard routine semen analysis involving sperm concentration, motility and morphology does not predict the implantation percentages in an ICSI cycle. Once sperm DNA fragmentation (SDA) methods were inducted they appeared to hold promise in forecasting good ART success. Although certain studies utilizing various techniques like TUNEL. SCSA, SCD proved a relation existed between DNA damage and implantation rates in ICSI but the same was contradicted by others. With this it was thought that bias between evaluation of ejaculate and motile sperm picked up for ICSI, as is known regarding absence of positive association of sperm motility and DNA fragmentation. Thus study by Casanovas et.al., tried to find if there is any correlation of single stranded (ssSDA) and double stranded (dsSDA) sperm DNA damage that might forecast ICSI success and utilizing Neutral Comet Assays along with help of time lapse technology they found that double stranded sperm influenced delay in embryo formation as seen by embryo kinetics and thus interfere with implantation rates. Reproduction of these findings might help in getting a standard for getting best embryos selected in ICSI utilizing SDA and time lapse microscopy.

scholarly journals Correlation of sperm DNA damage with blastocyst formation: systematic review and meta-analysis

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Prashanth K. Adiga ◽  
Srisailesh Vitthala ◽  
Shivaranjeni
Keyword(s):  
Dna Damage ◽  
Dna Fragmentation ◽  
Semen Analysis ◽  
Meta Analysis ◽  
Main Body ◽  
Sperm Dna Fragmentation ◽  
Blastocyst Formation ◽  
Male Factor ◽  
Sperm Dna Damage ◽  
Sperm Dna

Abstract Background The routine semen analysis fails to detect sperm DNA damage which contributes to the majority of male factor infertility. Sperm DNA fragmentation test (DFI) measures the sperm DNA damage. Blastocyst formation is an important step in IVF ± ICSI. At present, the literature lacks any data that correlates DFI and blastocyst formation. Main body of the abstract We searched MEDLINE and other databases till 2020 for the studies that reported on sperm DNA damage and blastocyst formation in assisted reproductive technology (ART). The outcomes analyzed were (1) a comparison of blastulation rates in high DFI and low DFI groups. (2) Comparison of blastulation rates in high DFI and low DFI groups based on (a) different sperm DNA fragmentation assays (COMET, SCD, SCSA, TUNEL), (b) different types of ART (IVF/IVF + ICSI/ICSI). 10 studies were included in this review. A non-significant increase in the blastocyst formation was observed in high DFI group (OR = 0.70; 95% CI = 0.4 to 1.21; P = 0.20) and with SCD and TUNEL assays. Short conclusion Our study emphasizes on sperm DNA fragmentation (sperm DNA damage) as an important marker of blastocyst formation. The results of this meta-analysis suggest that the high sperm DNA fragmentation may not adversely affect the blastocyst formation.

P–042 Impact of semen parameters, sperm DNA fragmentation and sperm aneuploidy in male infertility

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
M Maia ◽  
C Almeida ◽  
M Cunha ◽  
A Gonçalves ◽  
S S Soares ◽  
...  
Keyword(s):  
Male Infertility ◽  
Dna Fragmentation ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Sperm Dna Fragmentation ◽  
Male Age ◽  
Fertility Status ◽  
Sperm Aneuploidy ◽  
Sperm Dna ◽  
Aneuploidy Testing

Abstract Study question Should sperm aneuploidies and sperm DNA fragmentation (sDNAfrag) be included as valid tests in the routine investigation of male infertility? Summary answer Sperm DNA fragmentation was associated with male age, oligozoospermia (OZ), oligoteratozoospermia (OT), astenoteratozoospermia (AT) and oligoastenoteratozoospermia (OAT). Sperm aneuploidies were associated with OT and OAT. What is known already Semen parameters assist male infertility diagnosis and treatment, but sDNAfrag and aneuploidy analysis could add useful information, as abnormal values compromise fertility. To include these tests in the routine diagnosis it should be determined if behave as informative parameter and add information regarding the fertility status. For that, further studies comparing these tests to semen parameters are needed, since previous results are not consensual. Additionally, standardization of a sDNAfrag cut-off is needed, as different sample sizes and techniques originate distinct results. Also, until a standardization of the protocol is missing, a cut-off value should be defined for each laboratory. Study design, size, duration A retrospective and prospective investigation was performed, within a 12 years period (April 2007-December 2019). A total of 835 infertile males with a normal karyotype (46,XY) were included. Karyotyping and evaluation of sDNAfrag and sperm aneuploidies were made at a public Genetic unit. All normozoospermic (NZ) patients with a born child and patients whose infertility treatments were done due to female factors were selected from our database and used as controls (60 individuals). Participants/materials, setting, methods Semen analysis followed WHO–2010 guidelines. sDNAfrag was evaluated using the TUNEL assay. Sperm aneuploidies were detected using FISH (chromosomes 13, 18, 21, X, Y). Several tests were applied: correlations for linear associations between numerical variables, ANOVA for comparisons between means, Dunn-test for post-hoc comparisons. To determine the sDNAfrag cut-off value, the area under the ROC curve, sensitivity and specificity, were calculated, with the Youden-Index used to find a threshold that maximizes both sensitivity and specificity. Main results and the role of chance Regarding male age, it was observed a positive correlation with sperm concentration, a negative correlation with sperm vitality (VT) and hypoosmolality, and a positive correlation with sDNAfrag. Regarding sDNAfrag, it was observed negative correlation with sperm concentration, total progressive motility (TPM), morphology, VT and hypoosmolality. Regarding sperm aneuploidies, both total sperm aneuploidy and total sperm disomy exhibited a negative association with sperm concentration, TPM and morphology. It was also investigated whose groups of individuals could be indicated for sDNAfrag or sperm aneuploidy testing. The NZ group evidenced significant lower sDNAfrag, total sperm aneuploidy and total sperm disomy in relation to the non-NZ group. In the NZ group, sDNAfrag was significantly lower in relation to the OZ, OT, AT and OAT groups. The NZ group presented significant lower percentages of sperm aneuploidy in relation to the OT and OAT groups, and significant lower percentages of sperm disomy in relation to the OAT group. Additionally, sDNAfrag was positively correlated with total sperm aneuploidy and total sperm disomy. From the present large population, ROC curve analysis allowed estimating a cut-off value of 18.8% for the TUNEL-assay (sDNAfrag), with 0.658 of area under the curve, 53.9% sensitivity and 76.7% specificity. Limitations, reasons for caution Although presenting a high number of cases and strict controls, the present study was unable to include as controls healthy men with proven fertility. Additionally, the present study did not take into account life-style factors and male associated pathologies besides infertility. Wider implications of the findings: Semen parameters were shown to be negatively correlated with sDNAfrag and sperm aneuploidies. As sDNAfrag testing and sperm aneuploidy testing were associated with semen abnormalities and male age, it is suggested their inclusion in the routine evaluation of infertile men, thus adding important complementary information about the fertility status. Trial registration number Not Appliable

scholarly journals Contribution of Ayurveda in the Management of Ksheena Shukra Vikara with Special Reference to Asthenozoospermia: A Case Report

2021 ◽  
pp. 140-149
Author(s):  
Manju Mohan ◽  
Sawarkar Punam ◽  
Sawarkar Gaurav
Keyword(s):  
Case Report ◽  
Male Infertility ◽  
Sperm Motility ◽  
Clinical Symptoms ◽  
Sperm Count ◽  
Single Case ◽  
Semen Analysis ◽  
Male Factor ◽  
Sperm Analysis ◽  
Patient Reported

Background: Male Infertility is one of the burning issues now a day’s nevertheless disregarded reproductive health problems in India. Incidences of this issue expands day by day because of the disturbing lifestyle pattern. Almost 30-40-% of infertility cases found to be related to male factor. Asthenozoospermia is the most common identifiable anomaly related to male infertility found in semen analysis having reduced motility of sperm. Aim and Objectives: To assess the efficacy of Ayurvedic management (Shodhana and Shamana Chikitsa) in the management of Ksheena Shukra Vikara w.s.r. to Asthenozoospermia. Methods: It is a single case study. A 33-year-old male patient who was already diagnosed with Asthenozoospermia for three years approached to Pancharkarma OPD. Sperm motility was only 12%. The patient was treated with Shodhana Chikitsa (Vamana and Virechana with Mahatiktaka Ghritapana and Dashmooladi Niruha Vasti and Uttarvasti with Vidaryadi Ghrita followed by Shamana Chikitsa (Tab Neo Charak Pharmacy, Tab Addyzoa, Chandraprabha Vati, Paripathadi Kashaya, Ashwagandhadi Yamaka, Avipattikar Churna) approximately 3 months. After 3 months, patient-reported improvement. Results: Assessment of the patient with clinical symptoms and sperm analysis report was done following 3 months. Sperm motility increased up to 40% with increment in sperm count.  Conclusion: This case report provides us a guideline that infertility associated with Asthenozoospermia can be treated successfully by adopting basic Ayurveda Siddhanta's.

scholarly journals To analyse the semen for various parameters with special reference to lifestyle factors

2017 ◽  
Vol 6 (6) ◽  
pp. 2589
Author(s):  
Abhinav Aswal ◽  
Sangeeta Sharma ◽  
Rani Bansal ◽  
Anjali Khare
Keyword(s):  
Alcohol Consumption ◽  
Male Infertility ◽  
Prospective Study ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Seminiferous Tubules ◽  
Male Factor ◽  
Medical College ◽  
Accessory Sex Glands ◽  
Negative Effect

Background: Male factor is responsible for infertility in 23% cases. Semen analysis is the cornerstone of infertility evaluation as it provides information on the functional status of seminiferous tubules, epididymis and accessory sex glands. Reports in recent years has shown that incidence of male infertility has increased as a result of various factors such as lifestyle, environmental pollution and stress.Methods: This prospective study was conducted on patients reporting for semen analysis in Department of Pathology, Subharti Medical College. The duration of the study was from October 2014 to September 2016 with a study sample of 196 cases. Semen analysis was done by manual method according to WHO 2010 criteria.Results: According to fertility scoring, out of 196 cases, 51 (26%) were infertile cases. With respect to infertile cases 82.4% were alcoholic, 80.4% tobacco smokers, 25.5% were tobacco chewers. These results were statistically significant. Out of 45 cases of oligozoospermia 37 (82.2%) were alcoholic, 36 (80%) were tobacco smoker and 10 (22.2%) were tobacco chewers. Out of 54 cases of asthenozoosperma 38 (70.4%) were alcoholic, 37 (68.5%) were tobacco smoker and 11 (20.4%) were tobacco chewers.Conclusions: Alcohol consumption, tobacco smoking and tobacco chewing have a significant negative effect on the process of spermatogenesis, ultimately affecting sperm concentration, viability and motility. Hence clinician and fertility counselors need to be more focused to control infertility by modifying the life style factors. 

scholarly journals Cementing the relationship between conventional and advanced semen parameters

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Bashir M. Ayad ◽  
Ibukun P. Oyeyipo ◽  
Gerhard Van der Horst ◽  
Stefan S. Du Plessis
Keyword(s):  
Dna Fragmentation ◽  
Semen Analysis ◽  
World Health ◽  
Semen Parameters ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Linear Regression Models ◽  
Sod Activity ◽  
Sperm Analysis ◽  
The Relationship

Abstract Background Affordable conventional semen analysis remains a fundamental procedure to be performed routinely during the diagnosis of male infertility. Advanced semen analyses provide valuable clinical insights in treatment-related decision-making, but these are highly expensive and lack universal standardization. This study aimed at determining the relationship between conventional semen parameters, measured with assistance of computer-aided sperm analysis (CASA), and a set of advanced semen tests. Basic semen analysis (n = 124) was performed according to the World Health Organization (WHO) guidelines. Sperm DNA fragmentation and intracellular superoxide (O2−•) levels were assessed by flow cytometry. Seminal plasma thiobarbituric acid reactive substances (TBARS) levels as well as superoxide dismutase (SOD) and catalase (CAT) activity were measured by spectrophotometry. Spearman’s rank correlation coefficient was used, with significance set at p < 0.05. Results Semen pH correlated negatively with TBARS (p < 0.01). The proportions of total and progressively motile as well as rapid spermatozoa correlated positively with CAT activity (p < 0.05). Sperm viability correlated negatively with both O2−• (p < 0.05) and DNA fragmentation (p = 0.01), while normal morphology correlated negatively with O2−• levels (p < 0.05) and positively with CAT activity (p < 0.05). Straight-line velocity (VCL) and average-path velocity (VAP) correlated negatively with both O2−• (p < 0.01) and TBARS (p < 0.01). Amplitude of lateral head displacement (ALH) correlated negatively with O2−• (p < 0.01) and DNA fragmentation (p < 0.01), while its correlation with SOD activity was positive (p < 0.05). Conclusion The results obtained from this study support the validity of some CASA parameters as sensitive indicators of changes in sperm oxidative status and DNA integrity. Predicting advanced from conventional parameters through the building of linear regression models should be considered for future studies.

P–043 Six years’ retrospective statistical study (2013 – 2018) investigating the impact of Sperm DNA fragmentation on sperm analysis parameters

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
H Bahri ◽  
W Zidi ◽  
M Benkhalifa
Keyword(s):  
Dna Fragmentation ◽  
Sperm Motility ◽  
Sperm Morphology ◽  
Sperm Concentration ◽  
Structural Defects ◽  
Sperm Dna Fragmentation ◽  
Positive Correlation ◽  
Sperm Analysis ◽  
Sperm Dna ◽  
The Relationship

Abstract Study question What is the relationship between Sperm DNA fragmentation (SDF) levels and sperm analysis (Spermocytogramme) parameters results? Summary answer SDF level of patients with pathological spermocytogramme presents negative correlations to total spermatozoa mobility, vitality and concentration, and positive correlation to sperm morphology defects. What is known already The relationship between SDF and Sperm analysis parameters and especially sperm morphology needs to be more studied since few studies over the last years were focused on this relationship. However, abnormalities in these two parameters are considered as the most important biological indicators of male infertility. The pathogenesis of Teratozoospermia (&lt;4% morphologically normal sperm cells according to WHO 2010) is continuously increasing over the last decade according to several studies. In addition, SDF is also increasing over the years because of several factors such as pollution, stress and lifestyle changing. Study design, size, duration Retrospective study including 331 infertile patients undergoing SDF-index testing with Spermocytogramme from January 2013 – December 2018. Patients divided into two groups: 143 patients with normal-Spermocytogramme and 188 patients with pathological-Spermocytogramme. Each group includes patients with abnormal SDF levels (&gt;30%). Statistical analyzes were performed using SPSS22.0 for Windows-software. Kolmogorov–Smirnov-test for normality analysis and comparisons by Student-t-test or Mann–Whitney U-test, as appropriate. Pearson/Spearman’ tests for correlations were used as appropriate, P-value&lt;0.05 was considered as significant. Participants/materials, setting, methods 143 patients with normal Spermocytogramme (2.8% abnormal-SDF) vs 188 patients with pathological Spermocytogramme (10.6% abnormal-SDF). WHO–2010 instructions for sperm-analysis were used through Makler®-counting-chamber (Sefi-Medical Instrument Ltd) for sperm-concentration and motility-determination using Sperm-class-analyzer-software (CASA-system (Microptic®)) to detect sperm abnormalities. Normozoospermia was determined when sperm progressive-motility is ≥ 32%, sperm-concentration ≥15x106/mL, and sperm-morphology ≥4%. “Diff-Quick” staining-method for the coloration of the fixed-sperm-slides was used for Sperm-morphology analysis. GoldCyto Sperm®Kit (Goldcyto Biotech corp.) was used to analyze SDF. Main results and the role of chance SDF is significantly higher in pathological spermocytogramme’ patients than in normal spermocytogramme’ patients (17.02 ± 11.88 vs 12.16 ± 9.58 respectively). In patients with pathological spermocytogramme, SDF is negatively correlated to Progressive sperm motility (r= –0.137; p = 0.042), Total sperm motility (r= –0.153; p = 0.036), vitality (r=–0.140; p = 0.048) and concentration (r=–0.195; p = 0.007). In the other hand, SDF presented positive correlation with teratozoospermia and especially with sperm midpiece defects (r = 0.171; p = 0.02). However, SDF did not present any correlation with age, testosterone levels and total ejaculated sperm volume. However the latter was positively correlated to spermatozoa midpiece and head defects (r = 0.156; p = 0.034; r = 0.203; p = 0.006, respectively). These results are in accordance with García-Ferreyra et al. (2014) who found that men with abnormal spermatozoa morphology showed high levels of DNA fragmentation, Sá et al. (2015) who confirmed that semen with lower concentration, motility and morphology have higher levels of SDF and showed that sperm head staining patterns are correlated with the degree of SDF. In addition, recently the study of Jakubik-Uljaszstudy et al. (2020) could confirms our results when it concluded that detailed sperm structural defects coexist with abnormal nuclear sperm DNA dispersion and that men with teratozoospermia may have a higher risk for sperm DNA damage. Limitations, reasons for caution Our study is a retrospective statistical investigation that included patients attending to the laboratory for fertility diagnosis after a period of infertility. Meta-analyzes studies in addition to more prospective-randomized-controlled-trials with couples undergoing assisted-reproductive-treatments and in comparison with fertile men are needed to confirm the relationship between SDF and spermocytogramme defects. Wider implications of the findings: These results should interest andrologists, reproductive science fundamentalists and embryologists who want to improve the investigations on the origin of infertility especially when it comes from male side. Trial registration number Not applicable

scholarly journals Flow Cytometry Detection of Sperm DNA Fragmentation and Apoptotic Markers in the Semen of Infertile Males

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Huda Mossa Omran ◽  
Moiz Bakhiet ◽  
Volker Ehemann
Keyword(s):  
Flow Cytometry ◽  
Dna Damage ◽  
Male Infertility ◽  
Dna Fragmentation ◽  
Semen Analysis ◽  
Chromatin Condensation ◽  
B Cell Lymphoma ◽  
Sperm Dna Fragmentation ◽  
Molecular Defects ◽  
Sperm Dna

The effect of sperm molecular defects on fertilization and pregnancy outcome after assisted reproductive therapy (ART) is widely documented by both research and clinical societies. Sperm DNA fragmentation and abnormal chromatin condensation represent critical causes of male infertility. Advanced androgenic techniques for accurately identifying molecular defects help in selecting an appropriate treatment strategy. Additionally, specific markers of apoptosis are increasingly important in predicting male infertility. The ability of flow cytometry to estimate the quantity of sperm with DNA fragmentation or damage and multifactor measurements in immotile sperm have made this developed technique essential in fertility centers. The study is aimed at assessing the level of DNA fragmentation and apoptosis by measuring flow cytometry using new techniques. Flow cytometry analysis revealed a varying degree of DNA damage. It was able to quantify the degree of impairment even in samples with minimal DNA fragmentation. DNA damage was observed even in samples that were considered normal with a routine semen analysis. Flow cytometry was sensitive to changes in sperm apoptosis. Elevated p53 activity levels were associated with high DNA fragmentation. Meanwhile, B-cell lymphoma 2 (Bcl-2) activities showed a different pattern. In conclusion, flow cytometry for sperm DNA fragmentation and markers of apoptosis can be a valuable tool in assisted reproductive centers.

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