scholarly journals LUNG MORPHOLOGY IN MARAL FETUSES

Author(s):  
S.N. Chebakov ◽  
◽  
O.S. Mishina ◽  
Ye.D. Berdova ◽  
◽  
...  

Maral breeding is a promising branch of animal hus-bandry in the Altai Region and the Republic of Altai. Being semi-wild animals, marals travel long distances, mainly in mountainous areas in larch and pine forests and on high-altitude plateaus of the Republic of Altai. The following deer species range in the Altai Region and the Republic of Altai: Siberian musk deer(Moschus moschiferus), elk(Alces alces), sika deer(Cervus nippon), reindeer(Rangifer tarandus), and maral (Cervus elaphus sibiricus, Severtzov 1872). According to V.G. Lunitsyn, the regions of the Re-public of Altai account for 98% of all velvet antler products produced in Russia which are exported to the countries of the Asian-Pacific Region. The specimens were taken from maral fetuses of the age from 1 to 3 months obtained at forced slaughter. The fetuses were weighed, the lungs were extracted and weighed, and the specimens were for-malin-fixed (10-12% solution), washed in tap water;and slices were prepared by a freezing microtome. The slices were hematoxylin-eosin stained according to Van Gieson. The tissue specimens show that all orders of bronchioles in the lungs of maral fetuses, especially in the caudal lobes, are clearly expressed. In the bronchi of the lungs of fetuses at the age of 3 months, the muscle layer of smooth muscle cells is expressed. At this age, the spaces similar to alveo-lar passages are visible.The diameter of the alveoli in the form of tubes at this age is about 2-5 μm. In the micro-slides under study, vessels in the lungs of fetuses at the age of 1-3 months are characterized by rapid growth. Con-nective tissue elements surrounding the bronchial tubes with capillaries are formed.

2020 ◽  
Author(s):  
Shasha Fan ◽  
Jianxun Zhu ◽  
Min He ◽  
Nan Wang ◽  
Xiaoru Xu ◽  
...  

Abstract Background As a famous animal-derived herbal medicine, the pharmacological functions of velvet antler are widely recognized in the world. However, there are still great limitations on quality standard and control system of velvet antler. Methods In this research, microscopic observation, delayed luminescence (DL) and chemical analysis were used to evaluate the four grades of velvet antler slices obtained from Cervus nippon Temminck and Cervus elaphus Linnaeu. In addition, those three measurement platforms have been used to compared the two of the four grades among Cervus nippon Temminck, Cervus elaphus Linnaeu and Rangifer tarandus. Results We assessed the quality grades of velvet antler slices by using microscopic observation, delayed luminescence and chemical analysis. These techniques are able to indicate the difference of different grades of velvet antler slices to a certain extent, and has their own advantages, respectively. Conclusion As a new, rapid and systemic tool, DL provides a technique for studying the overall property of different grades of velvet antler slices, and the integrated assessment by measuring microscopic observation, DL and chemical components may provide a novel means to measure animal-derived herbal quality control.


1998 ◽  
Vol 244 (1) ◽  
pp. 123-134 ◽  
Author(s):  
A. M. Long ◽  
N.P. Moore ◽  
T. J. Hayden

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Laura Glendinning ◽  
Buğra Genç ◽  
R. John Wallace ◽  
Mick Watson

AbstractThe rumen microbiota comprises a community of microorganisms which specialise in the degradation of complex carbohydrates from plant-based feed. These microbes play a highly important role in ruminant nutrition and could also act as sources of industrially useful enzymes. In this study, we performed a metagenomic analysis of samples taken from the ruminal contents of cow (Bos Taurus), sheep (Ovis aries), reindeer (Rangifer tarandus) and red deer (Cervus elaphus). We constructed 391 metagenome-assembled genomes originating from 16 microbial phyla. We compared our genomes to other publically available microbial genomes and found that they contained 279 novel species. We also found significant differences between the microbiota of different ruminant species in terms of the abundance of microbial taxonomies, carbohydrate-active enzyme genes and KEGG orthologs. We present a dataset of rumen-derived genomes which in combination with other publicly-available rumen genomes can be used as a reference dataset in future metagenomic studies.


2000 ◽  
Vol 12 (S1) ◽  
pp. 419-422 ◽  
Author(s):  
Akira Homma ◽  
Jong Inn Woo ◽  
Helen F. K. Chiu ◽  
Kua E. Hoek

The recognition of behavioral and psychological symptoms of dementia (BPSD) varies in Asian countries. In India, for example, symptoms such as repetition, forgetfulness, anxiety, and agitation are simply ignored in the rural setting and are of no concern to family members; other symptoms, such as wandering, are easily tolerated. This cultural acceptance of certain BPSD, combined with the difficulty in locating a physician skilled in the diagnosis and treatment of these symptoms, limits their assessment and treatment in India (International Psychogeriatric Association, 1998). Fortunately, in a few Asian countries, empiric data on the frequency of BPSD have been obtained using formal assessment instruments.


1995 ◽  
Vol 1 (2) ◽  
pp. 149-155 ◽  
Author(s):  
Zabihollah Rezaee ◽  
Joseph Z. Szendi ◽  
Connie Shum ◽  
Robert C. Elmore

Author(s):  
Ch. N. Sambyla ◽  
N. M. Bessonova ◽  
R. B. Chysyma

The Republic of Tyva is a region in the geographical center of Asia, which located at the junction of the Siberian taiga and Central Asian desert-steppe landscapes, in a wide band of mountains and intermountain plains. The mountain-forest area of Tyva has long been considered a natural habitat for antler deer, one of which is the maral (Cervus elaphus sibiricus). In order to restore maral breeding and increase the number of marals in the former limits, marals of the Altai-Sayan breed have been imported to the Republic of Tyva from the Republic of Altai. The preservation of productive traits, reproductive function and the realization of the genetic potential of animals introduced to new climatic conditions largely depends on the ability of these animals to adapt to existing environments. We have assessed in this paper the natural resistance of the marals of Altai-Sayan breed during introduction in the Tyva Republic in comparison with the indicators of the marals have been bred in the Altai Republic. The researches have been carried out in 2019. The research material was blood samples of marals of Altai-Sayan breed imported to the Republic of Tuva (n=27) and marals of the same breed bred in the Republic of Altai (n=17). Studies have shown some deviations in the blood leukogram of imported marals, such as a decrease in the number of eosinophils and rod nuclear neutrophils (P < 0,001), the increase in the number of segmented nuclear neutrophils and lymphocytes (P < 0,05). The number of monocytes have been increased in 18,6 times compared to the Altai marals. The change in the number of monocytes exceeded the physiological norm by 24,7 %. The indicator of adaptation evaluation in imported marals had higher values (6,8), which were in 1,7 times higher than in marals bred in the Altai Republic (4,1), which indicates the intensity of adaptive mechanisms in imported animals during adaptation. Analysis of phagocytic activity and phagocytic index revealed intensive phagocytosis in imported marals: phagocytic activity – by 12,4 % (P < 0,05), phagocytic index – by 5,1 %, and the increase in the content of lysosomal and cationic proteins by 12,8 % (P < 0,05). Analysis of the bactericidal activity of blood serum has shown its lower level in imported animals (45,97±1,36 %), compared with marals of the same breed bred in the Altai Republic (52,19±2,15 at P < 0,05). Thus, according to most indicators of natural resistance marals of Altai-Sayan breed imported to Tyva have a fairly high level of natural protection, which indicates that they can be adapted to the natural climatic and feeding conditions of the Republic of Tyva.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 241-242
Author(s):  
Anna Krutikova ◽  
Natalia Dementeva

Abstract In the northern part of Russia, there are wild and domestic reindeer. There are four breeds of domestic reindeer in Russia: Nenets, Evenki, Even and Chukchi. The breeds are adapted to various climatic zones of habitat (tundra or taiga). Breeds vary in growth and live weight, as well as in areas of application. The aim was to study the polymorphism of the gene for growth hormone in the reindeer. The DNA was extracted by the phenolic method using the standard method using proteinase K from blood and tissues. Specially designed primers were used to amplify the site of growth hormone gene. As a result of amplification, a 422 bp fragment was obtained covering the region of the somatotropin gene containing 2 and 3 exons. The resulting amplification was sequenced on an Applied Biosystems 3500 Genetic Analyzer using the BigDye® Terminator v3.1 Sequencing Standard Kit according to the protocol. Four single-nucleotide polymorphisms of C12T, C72T, A122G, A235G were detected in the investigated region of the growth hormone gene (somatotropin). The positions of the found SNPs were determined from a similar section of the gene in a red deer (Cervus elaphus), the genome of which was completely sequenced. Two SNPs are in the intron, two are in the exon. The frequencies of genotypes and alleles were calculated in the six studied populations of domestic and wild reindeer. The results obtained in the study make it possible to draw conclusions about the genetic difference between the populations of wild and domestic reindeer of Russia, and between the domestic reindeer of different breeds. Theme АААА-А-18-118021590138-1.


2020 ◽  
Author(s):  
Xue Li ◽  
Le-Tai Yi ◽  
Ya-Qiong Bi ◽  
Lei Zhang ◽  
Ya-Hong Sun ◽  
...  

Abstract Background: The traditional medical practices of the Daur region are an important element in Chinese ethnomedicinal knowledge. However, relatively little recording of traditional Daur medicine (TDM) has been carried out. As it is gradually being replaced by traditional Chinese medicine (TCM) and modern medicine, further research is urgently needed. Methods: We collected ethnobotany and ethnozoology data about their local name, parts used and diseases to be treated, dosage, and route for administration through semi-structured interviews with 114 informants (18 sessions), focus group discussions (6 sessions), and extensive literature reviews. Medicinal samples and specimens were collected during field investigations from June 2015 to October 2018. The diseases and ailments reported as being treated were classified according to the International Classification of Diseases 11 th (ICD-11). The expression correlation of medicine classification, medicine, and disease was assessed using Cytoscape 3.6.1 software. Quantitative indices such as informant consensus factor (ICF) and use value (UV) were used to evaluate the importance of ethnomedicinal data. Results: We documented 30 plant species (from 19 families) and 21 animal species (from 11 families). In the past, the majority of plant species were collected from natural habitats, but now they are mainly cultivated. The most widely utilized plants were herbs (21 species), followed by shrubs (4), trees (3), and fungus (2). The most utilized groups of animals were mammals (14 species), followed by birds (5), amphibians (1), and reptiles (1). Medicines were mostly administered orally (43) but were also applied externally (11) or delivered via both routes (9). Informants indicated that medicines were prepared via decoction (21), grinding (11), boiling (10), extraction (8), and burning (7). Medicines were taken as a drink (37), eaten (16), or made into pills and powders (7), but could also be administered as an ointment/wash (6), wrap/dressing (5), in the nose, eyes, and mouth (4), or as a fumigate (2). The TDMs investigated in this study treated 76 human diseases or ailments classified under 13 disease categories, based on the ICD-11. The highest ICF (1.00) was reported for neoplasms and visual system diseases and childbirth or the puerperium, followed by injury, poisoning, or other diseases resulting from external causes (0.92), and symptoms, signs or clinical findings, not elsewhere classified (0.82). The most used medicinal species were Cervus elaphus , Cervus nippon , Capreolus , Gallus , Canis lupus familiaris , Betula platyphylla , and Artemisia integrifolia . Conclusions: A substantial body of ethnobotanical and ethnozoological knowledge could lead to the development of new medicines. Therefore, research into Daur ethnic medicines is urgently required The current research can make an important contribution to the body of knowledge about the national medicine of the Daur people. This is particularly important because the younger generation is not interested in learning and preserving their traditional medical practices. The information documented in the present study will provide a useful basis for future investigations into the ethnopharmacological and traditional knowledge of the Daur region.


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