scholarly journals Cracking the Metabolic engineering of bacteria: Review of methods involved in organic acid Production

2021 ◽  
Author(s):  
Manam Walait ◽  
Huda Rehman mir ◽  
Zainab Hassan ◽  
Javed Iqbal Wattoo
Keyword(s):  
Metabolic Engineering ◽  
Recombinant Dna ◽  
Product Formation ◽  
Background Information ◽  
Recombinant Dna Technology ◽  
Transport Pathway ◽  
Organic Acid Production ◽  
Nutritional Values ◽  
Food Industries ◽  
Average Body

Metabolic engineering is defined as recombinant DNA technology to improve specific biochemical reactions for product formation. We modify the metabolic processes of bacteria to get our desired food by metabolic engineering. Metabolic engineering will enhance these microorganisms' properties and their ability to produce a diverse number of products cost-effectively. To produce amino acids, we modify the central metabolic pathway, biosynthetic pathway, and transport pathway. In many food industries, the production of organic acids through different processes and techniques have proved very beneficial because of their widespread applications. In line with this information, the present review aimed to provide background information for researchers about genetically modified foods for increased food yield to fulfil the nutritional values for average body growth.

Industrial fermentations with (unstable) recombinant cultures

1982 ◽  
Vol 297 (1088) ◽  
pp. 617-629 ◽  
Keyword(s):  
Mixed Culture ◽  
Recombinant Dna ◽  
Novel Species ◽  
Oxygen Demand ◽  
Continuous Cultivation ◽  
Product Formation ◽  
Time Dependent ◽  
Recombinant Dna Technology ◽  
Fermentation Time ◽  
Full Sense

Recombinant DNA technology is yielding organisms with virtually a complete range of genetic stability, i.e. from organisms that appear to be entirely stable over the course of many fermentations to those that are so unstable that their half-life as novel species may be appreciably less than a conventional fermentation time. As such, a novel time-dependent mixed culture community is created, containing at a minimum the original recombinant microbe and a second strain, which may be denoted as a revertant in some partial or full sense. These two strains will differ in one or more important characteristics such as (desired) product formation rates, substrate utiliza­ tion abilities, morphology, biomass growth rates and oxygen demand. In this paper, I consider batch and continuous cultivation, and I use some plausible biological rate forms to explore implications in fermentor behaviour and product formation occa­ sioned by the existence of this particular form of ‘mixed culture’.

Enzymatic Synthesis of 15N-L-aspartic Acid Using Recombinant Aspartase from Escherichia Coli K12

2008 ◽  
Vol 59 (11) ◽  
Author(s):  
Iulia Lupan ◽  
Sergiu Chira ◽  
Maria Chiriac ◽  
Nicolae Palibroda ◽  
Octavian Popescu
Keyword(s):  
Amino Acids ◽  
Aspartic Acid ◽  
Enzymatic Synthesis ◽  
Large Scale ◽  
Recombinant Dna ◽  
Industrial Enzymes ◽  
Recombinant Dna Technology ◽  
Bacterial Fermentation ◽  
Natural Protein ◽  
Novel Method

Amino acids are obtained by bacterial fermentation, extraction from natural protein or enzymatic synthesis from specific substrates. With the introduction of recombinant DNA technology, it has become possible to apply more rational approaches to enzymatic synthesis of amino acids. Aspartase (L-aspartate ammonia-lyase) catalyzes the reversible deamination of L-aspartic acid to yield fumaric acid and ammonia. It is one of the most important industrial enzymes used to produce L-aspartic acid on a large scale. Here we described a novel method for [15N] L-aspartic synthesis from fumarate and ammonia (15NH4Cl) using a recombinant aspartase.

scholarly journals Protein-Engineered Polymers Functionalized with Antimicrobial Peptides for the Development of Active Surfaces

2021 ◽  
Vol 11 (12) ◽  
pp. 5352
Author(s):  
Ana Margarida Pereira ◽  
Diana Gomes ◽  
André da Costa ◽  
Simoni Campos Dias ◽  
Margarida Casal ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Peptides ◽  
Recombinant Dna ◽  
Biological Properties ◽  
Resistant Bacteria ◽  
Recombinant Dna Technology ◽  
Free Standing ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Antimicrobial Materials

Antibacterial resistance is a major worldwide threat due to the increasing number of infections caused by antibiotic-resistant bacteria with medical devices being a major source of these infections. This suggests the need for new antimicrobial biomaterial designs able to withstand the increasing pressure of antimicrobial resistance. Recombinant protein polymers (rPPs) are an emerging class of nature-inspired biopolymers with unique chemical, physical and biological properties. These polymers can be functionalized with antimicrobial molecules utilizing recombinant DNA technology and then produced in microbial cell factories. In this work, we report the functionalization of rPBPs based on elastin and silk-elastin with different antimicrobial peptides (AMPs). These polymers were produced in Escherichia coli, successfully purified by employing non-chromatographic processes, and used for the production of free-standing films. The antimicrobial activity of the materials was evaluated against Gram-positive and Gram-negative bacteria, and results showed that the polymers demonstrated antimicrobial activity, pointing out the potential of these biopolymers for the development of new advanced antimicrobial materials.

scholarly journals Biotin Production by Using Recombinant DNA Technology

1992 ◽  
Vol 38 (Special) ◽  
pp. 263-266
Author(s):  
O. IFUKU ◽  
S. HAZE ◽  
J. KISHIMOTO ◽  
M. YANAGI
Keyword(s):  
Recombinant Dna ◽  
Recombinant Dna Technology ◽  
Dna Technology

Introduction to Recombinant DNA

PEDIATRICS ◽  
1984 ◽  
Vol 74 (3) ◽  
pp. 408-411
Author(s):  
Stephen D. Cederbaum
Keyword(s):  
Inborn Errors Of Metabolism ◽  
Recombinant Dna ◽  
Phenylalanine Hydroxylase ◽  
Professional Lives ◽  
Recombinant Dna Technology ◽  
Inborn Errors ◽  
Dna Technology ◽  
Basic Vocabulary ◽  
The Moment ◽  
The Many

Seldom has a scientific or biomedical break-through evoked the awe, controversy, or sheer incredulity that has accompanied the developments in the field of recombinant DNA technology or more popularly, gene cloning and genetic engineering. Now little more than one generation after Avery, et al1 demonstrated that genes were encoded in DNA and Watson and Crick2 interpreted the structure of these molecules, genes are being cut, manipulated, and recombined to produce unprecedented new insights into genetics and molecular biology and the prospect of gene therapy. These developments have not occurred without anxiety to both scientists and laymen. At the moment, neither the most apocalyptic fears nor the most optimistic dreams appear to be imminent, although I believe that the dreams are closer to fulfillment than the fears. Recombinant DNA technology is already having great impact in hematology, oncology, endocrinology, immunology, and infectious disease and will soon play an important role in other medical subspecialities as well. In none, however, will it have quite the same impact as in genetics because DNA is the material that genetics "is all about." The cloning and study of phenylalanine hydroxylase is one of the first instances in which this technology has important implications in the diseases traditionally classified as inborn errors of metabolism. In order to understand and appreciate the presentation by Woo on phenylalanine hydroxylase as well as the many future papers that will play so vital a role in all of our professional lives, it is necessary to acquire the basic vocabulary of the field.

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