Phytochemical screening and evaluation of antioxidant activity of the stem bark of Anacardium occidentale L. and Vitellaria paradoxa C.F. Gaertn, two medicinal plants from Poro region (Northern of Côte d'Ivoire)

2021 ◽  
Vol 1 (2) ◽  
pp. 022-028
Author(s):  
Gboko Abiba Ouattara ◽  
Tiekpa Wawa Justine ◽  
Kamagaté Tidiane ◽  
Boni Ahoussi Pascal ◽  
Kabran Aka Faustin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Stem Bark ◽  
Anacardium Occidentale ◽  
Phytochemical Screening ◽  
Vitellaria Paradoxa ◽  
Dpph Method ◽  
Treatment Of Diabetes ◽  
Major Chemical ◽  
Chemical Groups

Anacardium occidentale and Vitellaria paradoxa are two medicinal plants traditionally used by Ivorian, particularly in the treatment of diabetes, arterial hypertension and diarrhea. The objective of this work was to evaluate the phytochemical constituents and the antioxidant activity of the stem barks of A. occidentale L. and V. paradoxa. The stem bark of both plants was used for the aqueous extraction. Major chemical groups were identified using the tube staining method followed by confirmation by thin layer chromatography (TLC). The antioxidant activity of the extracts was evaluated by DPPH method. The results of phytochemical screening of plant extracts revealed the presence of polyphenols, anthraquinones, tannins, sterols, terpenes and saponins. In addition, the evaluation of the antioxidant activity by spectrophotometry using the DPPH method showed that all extracts had significant and different antioxydant activity compared to the reference molecule (Vitamin C, IC50 = 0.80 ± 0.70 µg/mL) with respective IC50 of 0.42 ± 0.32 µg/mL for V. paradoxa and 0.87 ± 0.77 µg/mL for A. occidentale. V. paradoxa therefore has the best antioxydant activity. These results could explain the therapeutic virtues of A. occidentale and V. paradoxa in the treatment of various pathologies.

scholarly journals Etude Phytochimique et Activité Antioxydante des Extraits D’écorces de Tiges de Vitellaria Paradoxa C.F.Gaertn, Une Plante Médicinale Utilisée au Nord de la Côte d’Ivoire

2021 ◽  
Vol 17 (34) ◽  
pp. 241
Author(s):  
Kamagaté Tidiane ◽  
Gboko Abiba Ouattara ◽  
Koné Monon ◽  
Touré Abdoulaye ◽  
Ouattara Karamoko
Keyword(s):  
Antioxidant Activity ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Activité Antioxydante ◽  
Vitellaria Paradoxa ◽  
Cote D’Ivoire ◽  
Plante Médicinale ◽  
Côte D'ivoire ◽  
Dpph Method ◽  
Chemical Groups

Au Nord de la Côte d’Ivoire, Vitellaria paradoxa (karité) est une plante médicinale couramment utilisée contre de nombreuses pathologies. Le présent travail a pour objectif de déterminer les principaux constituants chimiques et d’évaluer l’activité antioxydante des extraits aqueux et hydro-éthanolique (70 %) d’écorces de tiges de cette plante. Les différents groupes chimiques ont été mis en évidence par la technique de coloration et de précipitation en tubes. La méthode de réduction du radical 2,2’-diphényl-1-picrylhydrazyle (DPPH) a permis d’évaluer le pouvoir antioxydant des extraits. Le criblage phytochimique a révélé la présence de principaux groupes chimiques suivants: alcaloïdes, anthraquinones, polyphénols totaux, terpènes et stérols, saponines et tanins. Le test de DPPH a montré une activité antioxydante importante des deux extraits, comparée à celle de la Vitamine C (CI50 =0,80±0,7 μg/mL) avec des CI50 respectives de 0,42±0,3 μg/mL pour l’extrait aqueux et 0,87±0,7 μg/mL pour l’extrait hydro-éthanolique. Ces résultats pourraient justifier l’utilisation traditionnelle de V. paradoxa pour le traitement de diverses pathologies dans de nombreuses régions de l’Afrique de l’Ouest.  In North of Côte d ’Ivoire, Vitellaria paradoxa (Shea tree) is a medicinal plant commonly used against many pathologies. The objective of this work is to determine phytochemical constituents and to evaluate antioxidant activity of aqueous and hydro-ethanolic extracts (70%) of Vitellaria paradoxa. Different chemical groups were highlighted by the tube staining and precipitation technique and the 2, 2’-diphényl-1-picrylhydrazyl (DPPH) method allowed the evaluation of the antioxidant power of the extracts. Phytochemical tests revealed the following chemical groups: alkaloids, anthraquinones, total polyphenols, terpenes and sterols, saponins and tannins. The DPPH test showed significant antioxidant activity of the two extracts compared to Vitamin C (IC50 = 0.80 ± 0.7 μg/mL) with IC50 respectively 0.42 ± 0.3 μg/mL for the aqueous extract and 0.87 ± 0.7 μg/mL for the hydro-ethanolic extract. These results could justify the traditional use of V. paradoxa in the treatment of various pathologies in many parts of West Africa.

scholarly journals Phytochemical Study and Antioxidant Activity of Annona muricata (Annonaceae) and Corchorus olitorius (Tiliaceae) Two Medicinal Plants from the Ivorian Pharmacopoeia Used in the Treatment of Diabetes

2021 ◽  
Vol 9 (1) ◽  
pp. 31-42
Author(s):  
Abba P. Obouayeba Obou C. Okou ◽  
Bognan J.A.A. Ackah Bi Tra L. Tra ◽  
Tanoh H. Kouakou Allico J.Djaman ◽  
Jean D. N guessan
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Gallic Acid ◽  
Aqueous Extract ◽  
Corchorus Olitorius ◽  
Annona Muricata ◽  
Phytochemical Screening ◽  
Phytochemical Study ◽  
Ic50 Value ◽  
Treatment Of Diabetes

Annona muricata and Corchorus olitorius are two medicinal plants whose leaves are widely used in Ivory Coast both in nutrition and for the treatment of various pathologies because of their phytochemical compositions. The objective of this study was to determine the phytochemicals and to assess the antioxidant property of the leaves of these two plants. The phytochemical study was carried out using tests for the identification and quantification of phytoconstituents. As for the study of antioxidant activity, it was carried out by measuring the anti-free radical activity either by ABTS or by DPPH. Qualitative phytochemical screening revealed the presence of flavonoids, polyphenols, saponins, sterols, catechetical tannins and terpenes. The quantitative phytochemical screening of the extracts showed that the content of flavonoids (329.10 ± 2.51 mg Eq quercetin/Kg) and that of polyphenols (82.09 ± 0.13 mg Eq gallic acid/g) of Annona muricata are higher than the content of flavonoids (178.45 ± 1.09 mg Eq quercetin/Kg) and that of polyphenols (67.98 ± 5.22 mg Eq gallic acid/g) of Corchorus olitorius. The results of the antioxidant activity by measurement of the ABTS radical shows that the aqueous extract of Annona muricata exhibits the best activity with an IC50 value of 1.79 ± 0.15 μM, while the aqueous extract of Corchorus olitorius records an IC50 value of 2.64 ± 0.05 μM. Similar results are observed with the measurement of DPPH. The various metabolites demonstrated have various therapeutic properties which may be involved in the treatment of diabetes. This would confirm the therapeutic use reported by the populations. However, the evaluation of the antimalarial, antibacterial and anti-inflammatory activities as well as the toxicity test of the aqueous extract of these plants would be necessary.

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

scholarly journals Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

2019 ◽  
Vol 79 (3) ◽  
pp. 452-459 ◽  
Author(s):  
F. R. G. Silva ◽  
T. M. S. Matias ◽  
L. I. O. Souza ◽  
T. J. Matos-Rocha ◽  
S. A. Fonseca ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Bioactive Metabolites ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Dpph Method

Abstract The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

scholarly journals IN-VIVO ANTI-DIARRHOEAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETHANOL STEM BARK EXTRACT OF VITELLARIA PARADOXA GAERTN F. (SAPOTACEAE)

2020 ◽  
Vol 4 (3) ◽  
pp. 247-251
Author(s):  
Z. Abdullahi ◽  
A. A. Jimoh ◽  
B. E. Patrick ◽  
M. I. Yakubu ◽  
D. Mallam
Keyword(s):  
Castor Oil ◽  
Lethal Dose ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Experimental Models ◽  
Bark Extract ◽  
Phytochemical Screening ◽  
Vitellaria Paradoxa ◽  
Stem Bark Extract

Different parts of Vitellaria paradoxa plant have many applications in ethno-medicine. Some of the uses of this plant include treatment of diarrhoea and other GIT disorders. In this study the antidiarrhoeal activity of the ethanol extract of Vitellaria paradoxa was evaluated using three experimental models: Castor oil-induced diarrhoea; small intestinal motility and intestinal fluid accumulation (enteropooling) models in mice. Five groups of five mice were used for each model. Group one mice received 10 ml/kg of distilled water, while groups 2, 3, and 4 received 125, 250 and 500 mg/kg of the extract orally respectively. Group 5 mice received Loperamide 5 mg/kg orally. Oral median lethal dose (LD50) of the extract was determined using OECD (2008) Guideline 425. Phytochemical studies were conducted using standard procedures. The LD50 was estimated to be greater than 5000 mg/kg body weight and there were no signs of mortality or visible signs of toxicity in all the mice treated. Phytochemical screening revealed the presence of carbohydrates, alkaloids, flavonoids, saponins, tannins, triterpenes, steroids, cardiac glycosides and anthraquinones glycosides. Extract showed a dose-dependent anti-diarrhoeal activity by reducing stool frequency and consistency. The extract at the higher doses significantly (p < 0.05) inhibited GIT motility and castor oil-induced enteropooling, comparable to that of the reference control drug Loperamide. The study showed that ethanol stem bark extract of Vitellaria paradoxa possess anti-diarrhoeal activity and thus justifies its ethno-medicinal use in the treatment of diarrhoea.

scholarly journals Utero-relaxing and anti-free-radical effects of hydro-ethanolic extracts of Desmodium ramosissimum in the wistar female rats

2021 ◽  
Vol 2 ◽  
pp. 6
Author(s):  
Hèzouwè Madjalani ◽  
Kossi Metowogo ◽  
Tcha Pakoussi ◽  
Aklesso Nabede ◽  
Essodjolon Kanabiya ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Medicinal Plants ◽  
Rural Areas ◽  
Public Health Problem ◽  
Stem Bark ◽  
Sub Saharan Africa ◽  
Female Rats ◽  
Phenolic Constituents ◽  
Ethanolic Extracts

Objectives: In sub-Saharan Africa, the therapeutic use of medicinal plants is of arousing interest. Reproductive disorders are a public health problem like everywhere else in the world. The population, especially in rural areas, uses medicinal plants for their beneficial effects. In Africa, the population uses these medicinal plants for their healthcare, including solving problems related to childbirth where there is an increase in the number of women known as “traditional birth attendants.” The effects of its medicinal plants relate to the various reproductive processes in women, from pregnancy to induction of labor, elimination of placental retention, and management of postpartum hemorrhage. To contribute to the enhancement of medicinal plants traditionally used in reproductive healthcare in Togo and to make information on their pharmacological effects available to the population, this work focused on the evaluation utero-relaxing and anti-free radical effects of extracts of Desmodium ramosissimum in the female rats of the Wistar strain. Material and Methods: The effects of hydro-ethanolic extracts of leaves, stem barks, and roots of D.ramosissimum were then tested on shreds Myometer of the female rats mounted in an organ vat and the antioxidant activity of these extracts tested was then determined. Results: The results show that hydro-ethanolic extracts from leaves, stem bark, and roots of D.ramosissimum cause a reduction in the frequency of spontaneous contractions of the uterine muscle. This reduction shows that these extracts have relaxing effects on the contraction of this muscle. The leaves extract showed better antioxidant activity compared to other extracts. Conclusion: This antioxidant activity is probably due to the phenolic constituents and its metabolites would be partly responsible for the pharmacological activities of D.ramosissimum. D.ramosissimum could thus be used to reduce uterine contractions in order to avoid Threats of Premature Labor.

scholarly journals Phytochemical Screening and Biocidal Studies Of Stem Bark Chrysophyllum Albidum (Linn) And Straw Aristolochia Ringens from Nigeria.

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Moses Owolabi
Keyword(s):  
Medicinal Plants ◽  
Cytotoxic Activity ◽  
Biological Activities ◽  
Stem Bark ◽  
Breast Tumour ◽  
Tumour Cells ◽  
Bacterial Activity ◽  
Liver Tumour ◽  
Phytochemical Screening ◽  
Health Maintenance

Introduction: Medicinal plants play a significant role in the health maintenance in underdeveloped countries, herbs and spices continue serve as a new sources for herbal medicines. Aim: The objective was to investigate phytochemical screening and biocidal activity on stem bark Chrysophyllum albidum (Linn) and straw Aristolochia ringens using chloroform. Methods: Chloroform extracts of C. albidum and A. ringens were obtained and screened for phytochemical constituents. The colour intensity or the precipitate formation was used as analytical responses to these tests. The Antimicrobial activities crude extracts were evaluated against some microorganisms with broth microdilution test. The extracts were screened for in-vitro cytotoxic activity against breast tumour and liver tumour cells. Results: The results of the phytochemical screening revealed the presence of alkaloids, saponins, steroids, anthraquinone and glycosides. C. albidum and A. ringens chloroform extracts revealed high quantities of alkaloid and flavonoid respectively. While cardiac glycoside and anthraquinone were found with low quantity in C. albidum and A. ringens extracts respectively. C. albidum showed promising bacterial activity against Escherichia coli and cytotoxic activity to liver tumour cells. While A. ringens, showed excellent bacterial activity against Bacillus cereus and good fungal activity against Candida albicans. A. ringens also showed cytotoxic to liver tumour and breast tumour cells. The observed antimicrobial and cytotoxic effects may be due to the antagonist or synergistic effect of the secondary metabolites identified in the extracts. Conclusion: Medicinal plants studied showed good potential biological activities that support the idea that traditional medicines remain useful healthcare in developing countries

scholarly journals Phytochemical screening and antioxidant activity of some medicinal plants’ crude juices

2020 ◽  
Vol 28 ◽  
pp. e00536
Author(s):  
Radwan S. Farag ◽  
Mohamed S. Abdel-Latif ◽  
Hanaa H. Abd El Baky ◽  
Layla S. Tawfeek
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Phytochemical Screening

scholarly journals UJI AKTIVITAS ANTIOKSIDAN SENYAWA FLAVONOID PADA KULIT BATANG TUMBUHAN GAYAM (Inocarpus fagiferus Fosb.)

Jurnal Kimia ◽  
2016 ◽  
Author(s):  
Made Hany Anastasia ◽  
Sri Rahayu Santi ◽  
Manuntun Manurung
Keyword(s):  
Antioxidant Activity ◽  
Ammonium Thiocyanate ◽  
Water Extract ◽  
Lipid Peroxide ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Chloroform Extract ◽  
Hydroxyl Groups ◽  
Tropical Plant ◽  
Dpph Method

Gayam (Inocarpus fagiferus Fosb) is a tropical plant whose stem bark has been used as traditional medicine. This research aims to identify the flavonoides isolate from stem bark of gayam and identify the class of flavonoids compound as antioxidant. Extraction of 1000 g stem bark powder with 8500 mL of 96 % ethanol to yield 30 g concentrated ethanol extract. Partition of 15 g extract ethanol-water (7:3) with chloroform and n-buthanol to yield 1,5 g concentrated chloroform extract, 5,87 g concentrated n-buthanol extract and 5,63 g concentrated water extract. The flavonoids test using Wilstatter and Bate-Smith Matcalf reagent showed that n-buthanol and water extract contain flavonoids compounds. Antioxidant activity of flavonoids tested with DPPH method and lipid peroxide ammonium thiocyanate method showed that n-buthanol extract had antioxidant activity with IC50 of 24 ppm and n-buthanol extract has ability to inhibit the formation of lipid peroxide with inhibition value of 63,04 %. Separation of n-buthanol extract using thin layer chromatography preparative with chloroform as mobile phase and silica gel GF254 as stationary phase obtained four fractions. Flavonoids test showed that fraction F3 contained flavonoids compounds. FTIR analysis indicated that isolate had functional groups such as  -OH , C=O, C-O alcohol, C=C aromatic, C-H aromatic and C-H aliphatic. UV-vis spectra showed 2 peaks at ? 310 nm  and  261 nm which indicated flavonoids group of  isolfavone with hydroxyl groups at C-5 and  C-7. Antioxidant activity test with DPPH method showed that the isolate had antioxidant activity with IC50 of 1 ppm.

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