A REVIEW ON NATURAL ANTI BIOFILM AGENTS FOR WOUND BIOFILM

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (10) ◽  
pp. 7-18
Author(s):  
Mahipriyaa S. R. ◽  
Baby Roselin R ◽  
Arjun K. ◽  
Nithyanth M. ◽  
Sankar V. ◽  
...  
Keyword(s):  
Natural Products ◽  
Growth And Development ◽  
Antibacterial Agents ◽  
Extracellular Polymeric Substances ◽  
Bacterial Resistance ◽  
Bacterial Biofilms ◽  
Wound Infections ◽  
Promising Candidate ◽  
Biofilm Growth ◽  
Micro Organisms

A biofilm may be a consortium of micro-organisms in which the cells of microbes attach to each other on a living or non-living surface inside a self-produced matrix of extracellular polymeric substances. The bacterial or fungal colonies invade the surface of the wounds, thereby delaying the healing cascade. The resistance is partially due to low metabolic rate of these colonies, which directly impacts the action of oral or parenteral antibiotics. Hence, there is an urgent need to develop antibacterial agents to regulate the biofilm growth and development. The last few decades have witnessed wide research studies attempting to investigate the anti-biofilm effects of natural products. This review will summarize the wound infections associated with biofilm, mechanisms of bacterial resistance due to biofilm and recent studies on discovery of natural products with their mechanisms for inhibiting various bacterial biofilms that can be a promising candidate which could provide novel strategies for biofilm-associated infections.

Efficacy of Usnea sp. Extracts in Preventing Biofilm Formation by Bacillus Species Isolated from Soaking Liquor Samples

2020 ◽  
Vol 115 (6) ◽  
pp. 222-229
Author(s):  
Didem Berber ◽  
İpek Türkmenoğlu ◽  
Meral Birbir ◽  
Nüzhet Cenk Sesal
Keyword(s):  
Biofilm Formation ◽  
Antibacterial Agents ◽  
Bacterial Resistance ◽  
Antimicrobial Agents ◽  
Biological Activities ◽  
Bacillus Species ◽  
Bacterial Biofilms ◽  
Bacterial Cells ◽  
Leather Industry ◽  
Acetone Extracts

Bacteria forms biofilm to be resistant to antibacterial agents and other unfavorable environment as compared to planktonic bacterial cells. Due to resistance of bacterial biofilms to commonly used antimicrobial agents and adverse effects of these biofilms in different industries, potential natural compounds which can inhibit bacterial biofilms have attracted more attention in recent years. Lichens are known to have unique secondary metabolites with various biological activities including anti-biofilm properties. Therefore, Bacillus toyonensis, Bacillus mojavensis, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus velezensis, Bacillus cereus, and Bacillus licheniformis, isolated from soak liquor samples in the previous study, were tested for their ability to form biofilm in this study. Biofilm-forming Bacillus species were detected as B. subtilis, B. amyloliquefaciens, and B. velezensis. The anti-biofilm effect of the acetone extracts of Usnea sp. was evaluated at various concentrations against these biofilm-forming isolates. The anti-biofilm effect of acetone extracts of Usnea sp. against B. subtilis and B. amyloliquefaciens was observed at the concentration of 5 µg/mL by inhibition ratios of 62.75% and 72.72%, respectively. In addition, biofilm formation of B. velezensis was inhibited by the treatment with 1.25 µg/mL extracts at a 62.69% inhibition rate. Biofilm formations of B. amyloliquefaciens and B. velezensis were also suppressed by the extracts at varying percentages of inhibition ranging between 10.11-43.69% and 21.25-46.35%, respectively. This study may provide an alternative approach to overcome the biofilm formation and bacterial resistance to the antibacterial agents in the leather industry.

scholarly journals Theoretical Evaluation of Novel Thermolysin Inhibitors from Bacillus thermoproteolyticus. Possible Antibacterial Agents

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 386
Author(s):  
Emilio Lamazares ◽  
Desmond MacLeod-Carey ◽  
Fernando P. Miranda ◽  
Karel Mena-Ulecia
Keyword(s):  
Antibacterial Agent ◽  
Antibacterial Agents ◽  
Bacterial Resistance ◽  
Antihypertensive Agents ◽  
Dynamics Simulation ◽  
Ligand Efficiency ◽  
Promising Candidate ◽  
Theoretical Evaluation ◽  
Gram Positive Bacteria ◽  
Poisson Boltzmann

The search for new antibacterial agents that could decrease bacterial resistance is a subject in continuous development. Gram-negative and Gram-positive bacteria possess a group of metalloproteins belonging to the MEROPS peptidase (M4) family, which is the main virulence factor of these bacteria. In this work, we used the previous results of a computational biochemistry protocol of a series of ligands designed in silico using thermolysin as a model for the search of antihypertensive agents. Here, thermolysin from Bacillus thermoproteolyticus, a metalloprotein of the M4 family, was used to determine the most promising candidate as an antibacterial agent. Our results from docking, molecular dynamics simulation, molecular mechanics Poisson–Boltzmann (MM-PBSA) method, ligand efficiency, and ADME-Tox properties (Absorption, Distribution, Metabolism, Excretion, and Toxicity) indicate that the designed ligands were adequately oriented in the thermolysin active site. The Lig783, Lig2177, and Lig3444 compounds showed the best dynamic behavior; however, from the ADME-Tox calculated properties, Lig783 was selected as the unique antibacterial agent candidate amongst the designed ligands.

scholarly journals An In Vitro Coumarin-Antibiotic Combination Treatment of Pseudomonas aeruginosa Biofilms

2021 ◽  
Vol 16 (1) ◽  
pp. 1934578X2098774
Author(s):  
Jinpeng Zou ◽  
Yang Liu ◽  
Ruiwei Guo ◽  
Yu Tang ◽  
Zhengrong Shi ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Drug Resistance ◽  
Combination Treatment ◽  
Crude Extract ◽  
Biofilm Formation ◽  
Bacterial Resistance ◽  
Antibacterial Properties ◽  
Biofilm Growth ◽  
Antibiotic Combination

The drug resistance of Pseudomonas aeruginosa is a worldwide problem due to its great threat to human health. A crude extract of Angelica dahurica has been proved to have antibacterial properties, which suggested that it may be able to inhibit the biofilm formation of P. aeruginosa; initial exploration had shown that the crude extract could inhibit the growth of P. aeruginosa effectively. After the adaptive dose of coumarin was confirmed to be a potential treatment for the bacteria’s drug resistance, “coumarin-antibiotic combination treatments” (3 coumarins—simple coumarin, imperatorin, and isoimperatorin—combined with 2 antibiotics—ampicillin and ceftazidime) were examined to determine their capability to inhibit P. aeruginosa. The final results showed that (1) coumarin with either ampicillin or ceftazidime significantly inhibited the biofilm formation of P. aeruginosa; (2) coumarin could directly destroy mature biofilms; and (3) the combination treatment can synergistically enhance the inhibition of biofilm formation, which could significantly reduce the usage of antibiotics and bacterial resistance. To sum up, a coumarin-antibiotic combination treatment may be a potential way to inhibit the biofilm growth of P. aeruginosa and provides a reference for antibiotic resistance treatment.

scholarly journals Natural products potential of Dictyoceratida sponges‐associated micro‐organisms

2021 ◽  
Author(s):  
E.R. Abdelaleem ◽  
M.N. Samy ◽  
U.R. Abdelmohsen ◽  
S.Y. Desoukey
Keyword(s):  
Natural Products ◽  
Micro Organisms

Antibiotic adjuvants: a promising approach to combat multidrug resistant bacteria

2021 ◽  
Vol 22 ◽  
Author(s):  
Namita Sharma ◽  
Anil K. Chhillar ◽  
Sweety Dahiya ◽  
Pooja Choudhary ◽  
Aruna Punia ◽  
...  
Keyword(s):  
Drug Targets ◽  
Pathogenic Bacteria ◽  
Antibacterial Agents ◽  
Bacterial Resistance ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Antibacterial Drugs ◽  
Effective Strategies ◽  
The Impact ◽  
Emergence Of Resistance

The escalating emergence and prevalence of infections caused by multi-drug resistant (MDR) pathogenic bacteria accentuate the crucial need to develop novel and effectual therapeutic strategies to control this threat. Recent past surprisingly indicates a staggering decline in effective strategies against MDR. Different approaches have been employed to minimize the effect of resistance but the question still lingers over the astounding number of drugs already tried and tested to no avail, furthermore, the detection of new drug targets and the action of new antibacterial agents against already existing drug targets also complicate the condition. Antibiotic adjuvants are considered as one such promising approach for overcoming the bacterial resistance. Adjuvants can potentiate the action of generally adopted antibacterial drugs against MDR bacterial pathogens either by minimizing the impact and emergence of resistance or improving the action of antibacterial drugs. This review provides an overview of mechanism of antibiotic resistance, main types of adjuvants and their mode of action, achievements and progression.

Antimicrobial peptide HPA3NT3-A2 effectively inhibits biofilm formation in mice infected with drug-resistant bacteria

2019 ◽  
Vol 7 (12) ◽  
pp. 5068-5083 ◽  
Author(s):  
Jong-Kook Lee ◽  
Loredana Mereuta ◽  
Tudor Luchian ◽  
Yoonkyung Park
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Peptide ◽  
Biofilm Formation ◽  
Extracellular Polymeric Substances ◽  
Resistant Bacteria ◽  
Bacterial Biofilms ◽  
Drug Resistant ◽  
Drug Resistant Bacteria ◽  
Serious Infections

Bacterial biofilms formed through secretion of extracellular polymeric substances (EPS) have been implicated in many serious infections and can increase antibiotic resistance by a factor of more than 1000.

scholarly journals Myostatin Promotes Osteoclastogenesis by Regulating Ccdc50 Gene Expression and RANKL-Induced NF-κB and MAPK Pathways

2020 ◽  
Vol 11 ◽  
Author(s):  
Xin Zhi ◽  
Qian Chen ◽  
Shaojun Song ◽  
Zhengrong Gu ◽  
Wenqiang Wei ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Growth And Development ◽  
Essential Role ◽  
Gene Overexpression ◽  
Promising Candidate ◽  
Mapk Pathways ◽  
Candidate Target ◽  
Type Receptor

Myostatin is a crucial cytokine that is widely present in skeletal muscle and that negatively regulates the growth and development of muscle cells. Recent research has shown that myostatin might play an essential role in bone metabolism. In RAW264.7 cells and bone marrow monocytes (BMMCs), myostatin activates the expression of the II type receptor ActR II B. Here, we report that myostatin significantly promoted RANKL/M-CSF-induced osteoclastogenesis and activated NF-κB and MAPK pathways in vitro via the Ccdc50 gene. Overexpression of myostatin promoted osteoclastogenesis and osteoclastogenesis-related markers including c-Src, MMP9, CTR, CK, and NFATc1. Specifically, myostatin increased the phosphorylation of Smad2, which led to the activation of NF-κB and MAPK pathways to activate osteoclastogenesis. Ccdc50 was identified as a gene whose expression was highly decreased in osteoclastogenesis upon myostatin treatment, and it could inhibit the function of myostatin in osteoclastogenesis by blocking NF-κB and MAPKs pathways. Our study indicates that myostatin is a promising candidate target for inhibiting RANKL-mediated osteoclastogenesis and might participate in therapy for osteoporosis, and that the Ccdc50 gene plays a significant role in the regulatory process.

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