scholarly journals Effect of Topical Application of Different Substances on Fibroplasia in Cutaneous Surgical Wounds

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Andreza Miranda Abreu ◽  
Dhelfeson Willya Douglas Oliveira ◽  
Sandra Aparecida Marinho ◽  
Nádia Lages Lima ◽  
João Luiz de Miranda ◽  
...  

Background. Fibroblasts on the edges of a surgical wound are induced to synthesize collagen during the healing process which is known as fibroplasia. Objective. The aim of this study was to determine the effect of the application of different substances on fibroplasia of cutaneous surgical wounds on rats. Materials and Methods. 48 Wistar rats were divided into three groups. A surgical wound 1 cm in diameter and 1  mm in depth was created on the dorsum of each animal. The surgical wounds were submitted to the topical application of an alcoholic extract of 30% propolis, 70% alcohol, or 0.001% dexamethasone in a cream base every 12 hours. The animals were sacrificed three, seven, 14, and 28 days postoperatively. The specimens were histologically processed and stained with Masson’s trichrome. The assessment of fibroplasia was performed using a scoring system: (1) 5 to 25% collagen deposition; (2) 26 to 50% collagen deposition; (3) 51 to 75% collagen deposition; (4) more than 75% collagen deposition. Results. There were statistically significant differences in collagen deposition between the substances at all postoperative evaluation times. Conclusion. Propolis and alcohol promoted greater collagen deposition in surgical wounds than dexamethasone.

2020 ◽  
Vol 41 (Supplement_1) ◽  
pp. S168-S169
Author(s):  
Reza Jalili ◽  
Myriam Maude Verly ◽  
Breshell Russ ◽  
Ruhangiz T Kilani ◽  
Aziz Ghahary

Abstract Introduction In large skin injuries, lack of matrix deposition impedes timely healing process. The longer a wound remains open, the greater is the risk of infection, non-healing, and other complications. It is therefore crucial to find effective means to promote rapid closure of skin defects. Our group has previously developed a liquid in situ-forming nutritional scaffold, known as MeshFill (MF). MF has been previously proven to be very effective in accelerating the wound repair process, notably that of complex wounds. However, MF is limited in its application to deep and tunnelling wounds, and requires reconstitution with a solvent as well as maintenance at cold temperature until application. To address these limitations, our group has developed a powdered form of MF for rapid topical application on superficial skin injuries such as dehisced surgical wounds and burn injuries. Methods Our goal was to investigate whether a powdered form of MF could be directly applied onto the wounds to accelerate healing. Ideally, powdered MF would absorb the moisture within the wound environment and reconstitute into the gel form in situ. We examined the efficacy of powder MF (PMF) compared to reconstituted gel MF (GMF) and to a standard dressing protocol. To do so, splinted full thickness wounds were generated on the back of mice and treated with either PMF or GMF or were bandaged with no treatment (NT). The healing process was monitored until wounds were fully closed. Clinical wound measurements and histological assessments were performed to compare different treatment regimens. Results Application of both PMF and GMF accelerated wound epithelialization at days 7 and 14, compared to NT, and had faster wound closure times. On average, the PMF treatments healed 17% faster than the NT control, and the GMF treatments healed 21% faster than the NT control. No significant difference between PMF and GMF was found for any outcomes. Additionally, our results suggest that epidermis formation was more effective in P and MF conditions compared to NT. Conclusions These findings suggest that topical application of a powdered form of MeshFill is as effective as standard reconstituted MeshFill gel in accelerating the healing process of skin injuries. Applicability of Research to Practice Topical application of a powdered scaffold may be a very convenient and practical method for rapid treatment of large superficial wounds such as dehisced surgical wounds, burn injuries, and filling gaps in meshed skin grafts.


2020 ◽  
pp. 138-140
Author(s):  
О.М. Kovalenko

Background. A wound is a defect of skin and underlying tissues that can be caused by many factors. Wound care is more than just changing dressings. Different wounds require different approaches and care. Optimal wound care supports the natural healing process in an effective but gentle way. Wounds are classified into acute and chronic, as well as cut, scalped, chopped, stabbed, bruised, torn, bitten, gunshot, and surgical. Surgical wounds are distinguished by the fact that they are applied specifically, for medical or diagnostic purposes, in special aseptic conditions, with minimal tissue trauma, in conditions of anesthesia, with thorough hemostasis and joining of separated anatomical structures with sutures. Objective. To describe the modern approach to wound care. Materials and methods. Analysis of literature data on this topic. Results and discussion. Types of wound healing include primary tension healing, delayed primary tension healing, secondary tension healing, and scab healing. When caring for surgical wounds, primary protection against microorganisms is important. In this case, sterile dressings play an important role, for example, a medical surgical patch with an absorbent pad (Yu-Fix, “Yuria-Pharm”). The pad is characterized by high hygroscopicity, does not stick to the wound and does not leave fibers in the wound. The frequency of dressings changing depends on the healing process and the amount of exudate released from the wound. For festering wounds, the dressing should be changed daily and sometimes more often. Hands and gloves should be worn before bandaging. After removing the gloves, the hands are also treated with an antiseptic (Gorosten, “Yuria-Pharm”, a decamethoxine-based antiseptic). The use of Gorosten in medical institutions for prophylactic purposes is indicated for disinfection of staff hands in order to prevent the spread of transient microflora. Sutures from the surgical wound are removed after the onset of epithelialization, when the wound is covered with a thin protective film. However, in this period there are still wound channels from the threads, so after that it is necessary to treat the wound several times with antiseptic (Dekasan, “Yuria-Pharm”). Signs of local infection of the wound are redness, excess exudate, sometimes – with the addition of pus, odor, pain in the area of injury, fever, edema. Sometimes postsurgical wound suppuration occurs even with proper care due to weak immunity or rejection of surgical suture material. If there is suppuration, it is advisable to switch to dressings with Dekasan and hyperosmolar antibacterial ointments. Unlike 2 % povidone-iodine, which causes severe inhibition of granulation in an open wound, Dekasan does not damage granulation tissue. Surgical treatment, lavage, drainage, antibiotics, laser or ultrasound may also be required. After removing the signs of local inflammation, secondary sutures are applied to the wound or the edges of the wound are connected with the help of adhesive plaster. Before starting the wound care procedure, it is necessary to assess the condition of the wound bed, the nature of the exudate, the condition of the tissues around the wound, pain, wound size. Wound management should involve a multidisciplinary approach. For example, an endocrinologist, a diabetic foot specialist, a purulent surgery surgeon, a vascular surgeon, and a nurse are involved in the treatment of ulcerative defects in patients with diabetes. In the treatment of wounds it is necessary to maintain a humid environment in the wound, maintain a constant temperature without hypothermia, provide adequate drainage and not too tight tamponade, use additional means of healing (eg, unloading the foot in the treatment of foot ulcers in patients with diabetes). Conclusions. 1. Optimal wound care supports the natural healing process in an effective but gentle way. 2. When caring for surgical wounds, primary protection against the effects of microorganisms is important. 3. Sutures from the surgical wound are removed after the onset of epithelialization, when the wound is covered with a thin protective film. 4. If there is suppuration, it is advisable to switch to dressings with Dekasan and hyperosmolar antibacterial ointments. 5. Wound management should involve a multidisciplinary approach.


2020 ◽  
Vol 10 ◽  
Author(s):  
Gerard Vicente Dantas de Medeiros ◽  
Tiago da Silva Teófilo ◽  
Antonio Salatino ◽  
Giuseppina Negri ◽  
Carmen E. P. Jara ◽  
...  

Background : The use of red propolis has antimicrobial, anti-inflammatory, antioxidant activity results, and provides local conditions that optimize events involved in the healing process, accelerating tissue repair. Objective: To evaluate the chemical composition, antioxidant activity, cytotoxic effects, and healing potential of red propolis hydroethanolic extract (RPHE) on the surgical wounds of cats submitted ovarisalpingohisterectomy. Methods: Chemical analyses of RPHE were carried out using HPLC-DADESI-MS/MS. Antioxidant activity was evaluating using the DPPH (2,2-diphenyl-1-picrilhydrazyl) method, and cytotoxicity was evaluated using the MTT colorimetric method. The healing effect of the extract was evaluated in 60 domestic cats (Felis catus) divided into three groups: GI: treated with topical application RPHE; GII: received topical application of commercial rifamycin-based solution, and GIII: treated with topical application of 0.9% NaCl physiological solution. Surgical wounds were evaluated for clinical, macroscopic, and histological aspects. Results: The RPHE showed 26 flavonoid and high antioxidant activity. Propolis extract had no cytotoxic effect on any of the cell lines tested. High cell viability percentages were observed, ranging from 80.0% to 94.4% for L929 fibroblasts and 78.0% to 91.5% keratinocytes (Hacat). Red propolis had a positive influence on the healing of wounds in cats submitted to ovarisalpingohisterectomy, because it promoted less intense inflammatory response, rapid closure of the wound, early deposition of collagen and reepithelization. Conclusion: The RPHE presented rich in flavanoids, high antioxidant activity, did not exert cytotoxic effect and promoted the acceleration of healing of surgical wounds.


2021 ◽  
Vol 73 (6) ◽  
pp. 1315-1322
Author(s):  
M.C.P. Gouveia ◽  
B.W. Minto ◽  
L.F. Sargi ◽  
R.L. Souza ◽  
J.M. Pazzini ◽  
...  

ABSTRACT The aim of this study was to evaluate the topical application of alcoholic extracts of Dipteryx alata Vogel almonds and bark in skin wound healing in mice. Fifty-four C57BL/6 mice were equally distributed into three groups: Control, Almond, and Bark. A 9 mm skin fragment was resected from the dorsal region of the animals’ thorax. The wounds were submitted to topical application of base cream (vehicle), 10% hydroalcoholic almond extract, or bark extract twice a day. Macroscopic, histological, and immunohistochemical evaluations were conducted on the 7th, 14th, and 21st postoperative days. No significant difference was observed regarding skin wound area among groups, with the parameter presenting only a temporal effect on healing (p>0.05). The almond and control groups exhibited more intense collagenization than the bark group (p<0.05). Dipteryx alata Vogel showed to be inert in the wound healing process in mice.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 25-26
Author(s):  
Nicholas W Dias ◽  
Claire L Timlin ◽  
Stefania Pancini ◽  
Nicholas W Dias ◽  
Zackary Seekford ◽  
...  

Abstract The objective of this study was to evaluate changes in vaginal pH of beef cows enrolled in a 7-d CO-Synch+controlled internal drug release (CIDR) protocol and its effects on pregnancy. A total of 46 multiparous beef cows with no signs of vaginitis were enrolled. Individual vaginal flushes were collected from cows on D0, D7 and D10. Individual flushes were immediately analyzed for pH with a portable pH meter. As evidence of vaginitis, a CIDR score was determined at CIDR removal (D7), following a 1 to 4 scoring system: 1 = clean, no secretion; 2 = clean, clear secretion; 3 = purulent secretion; 4 = purulent secretion and blood. Pregnancy status was determined by rectal ultrasonography approximately 40 days after TAI. No cows had CIDR scores of 1 or 4, 19.6% of cows had a score of 2 and 80.4% a score of 3. Vaginal pH increased from D0 (6.88±0.007) to D7 (7.32±0.047), regardless of CIDR score (P &lt; 0.001). Cows with a CIDR score 2 had greater pH on D7 (7.35±0.084) than on D0 (6.90± 0.14; P = 0.001), and pH on D10 was intermediate (7.14±0.14; P = 0.14). Cows with CIDR score 3 had reduced pH on D10 (7.09±0.07) when compared to D7 (7.29±0.04; P = 0.003), but greater pH on D10 than on D0 (6.87±0.07; P = 0.007). There was a pregnancy by day interaction for pH values (P = 0.046). Pregnant cows had greater vaginal pH in comparison with non-pregnant cows on D0 (6.99±0.081 and 6.68±0.09, respectively; P = 0.01) and D10 (7.26±0.08 and 6.89±0.09, respectively; P = 0.006), but not on D7 (7.34±0.05 and 7.27±0.06, respectively; P = 0.38). Non-pregnant cows had reduced vaginal pH on D10 in comparison to D7 (6.89±0.09 and 7.27±0.06, respectively; P &lt; 0.001), whereas pregnant cows had similar vaginal pH on D10 and D7 (7.26±0.08 and 7.34±0.05, respectively; P = 0.35). We conclude that CIDR increases vaginal pH overtime and greater pH at TAI is beneficial to fertility.


Author(s):  
Claudio Spinelli ◽  
Alessia Bertocchini ◽  
Gianmartin Cito ◽  
Marco Ghionzoli ◽  
Silvia Strambi

Abstract Purpose The purpose of the study is to evaluate results and outcomes in a long-time follow-up period, by performing a novel testicular fixation procedure, known as “fat anchor orchidopexy” (FAO), for the treatment of palpable low inguinal undescended testis. Materials and methods We retrospectively reviewed all patients who underwent scrotal orchiopexy technique, from May 2013 to May 2019, at the Pediatric Surgery Division of Department of Surgical Pathology, University of Pisa (Italy). FAO (Spinelli’s technique) consists in anchoring the testicles to sub-scrotal fat with a single trans-scrotal incision. All the patients enrolled had history of unilateral or bilateral undescended testis. Data collected included patient’s age, operative times and complications. Results A total of 150 children with cryptorchidism were treated using a single trans-scrotal orchiopexy. Of them, 130 patients (86.7%) had unilateral undescended testis and 20 (13.3%) bilateral cryptorchidism. Mean patient’s age was 21 months (range: 14–28 months). All the procedures were planned in a day-surgery setting. Trans-scrotal orchiopexy was successful in all cases and no patients required an additional groin incision. No intraoperatively and postoperatively major complications were observed. Patients’ post-operative pain was mild (mean pediatric visual analog scale = 2). In all cases, the healing process was rapid and no surgical wounds infections were reported during the post-operative period, referring excellent cosmesis results. During a mean 48-month follow-up period, no testicular retraction, recurrence or testis atrophy was reported. Conclusion The original Spinelli’s technique (FAO) proves to be a safe and effective method for the treatment of palpable or distal-to-external-inguinal-ring testes. No immediate and delayed post-surgery complications were reported. In all cases, the anchored testicle remained in the scrotal position with normal vascularization. This novel surgical technique could give better options for scrotal fixation in case of low-lying cryptorchid testes.


2018 ◽  
Vol 5 ◽  
pp. 5 ◽  
Author(s):  
Hanan Kamel ◽  
Richard R.A. Syms ◽  
Evdokia M. Kardoulaki ◽  
Marc Rea

An implantable sensor for monitoring surgical wounds after bowel reconstruction is proposed. The sensor consists of a coupled pair of 8-element magneto-inductive ring resonators, designed for mounting on a biofragmentable anastomosis ring to give a local increase in signal-to-noise ratio near an annular wound during 1H magnetic resonance imaging. Operation on an anti-symmetric spatial mode is used to avoid coupling to the B1 field during excitation, and a single wired connection is used for MRI signal output. The electrical response and field-of-view are estimated theoretically. Prototypes are constructed from flexible elements designed for operation at 1.5 T, electrical responses are characterized and local SNR enhancement is confirmed using agar gel phantoms.


2012 ◽  
Vol 2012 ◽  
pp. 1-6
Author(s):  
Abdulmohsen H. Al-Elq ◽  
Mir Sadat-Ali ◽  
Mohamed Elsharawy ◽  
Ibrahim Al-Habdan ◽  
Fatin Othman Al-Aqeel ◽  
...  

Objective. Diminished wound healing is a common problem in diabetic patients due to diminished angiogenesis. SHMSP was found to promote angiogenesis. The present study was carried out to examine the effect of this peptide in healing of wounds in diabetic rabbits.Materials and Methods. Twenty male New Zealand rabbits were used in this study. Diabetes mellitus was induced and the rabbits were randomly divided into two equal groups: control group and peptide group. A-full thickness punch biopsy was made to create a wound of about 10 mm on the right ears of all rabbits. Every day, the wound was cleaned with saline in control groups. In the peptide group, 15 mg of SHMSP was applied after cleaning. On day 15th, all animals were sacrificed, and the wounds were excised with a rim of 5 mm of normal surrounding tissue. Histo-pathological assessment of wound healing, inflammatory cell infiltration, blood vessel proliferation, and collagen deposition was performed.Results. There were no deaths among the groups. There was significant increase in wound healing, blood vessel proliferation and collagen deposition, and significant decrease in inflammatory cell infiltration in the peptide group compared to the control group.Conclusion. Topical application of SHMSP improves wound healing in diabetic rabbits.


2017 ◽  
Vol 50 (2) ◽  
pp. 71
Author(s):  
Meircurius Dwi Condro Surboyo ◽  
Ira Arundina ◽  
Retno Pudji Rahayu

Background: Traumatic ulcers in patients with diabetes mellitus may experience delayed healing despite their diabetic condition being controlled. Liquid smoke coconut shell containing phenolic compounds can potentially accelerate the healing process. One healing process indicator is the increased number of fibroblasts, another being the increased amount of collagen. Purpose: This study aimed to analyze the amount of collagen in traumatic ulcers in diabetics after application of liquid smoke coconut shell. Methods: Alloxan was induced in twenty-four male Wistar rats as models of diabetes mellitus. A traumatic 10 mm ulcer was made along the labial fornix incisive inferior with a round, stainless steel blade before liquid smoke coconut shell and benzydamine hydrochloride (as the control) was administrated once a day. A biopsy of the labial fornix incisive inferior was subsequently performed after the topical application for 5 and 7 days. Histological assessment was conducted to analyze the amount of collagen by means of Masson Trichome staining. Results: Histologically, the topical application of liquid smoke coconut shell for 5 days significantly increased the amount of collagen, higher than that of benzydamine hydrochloride as the control (p=0.006) (p<0.05). Meanwhile, the topical application of liquid smoke coconut shell for 7 days made the concentration of collagen no significantly different from that of benzydamine hydrochloride as the control (p=0.156) (p>0.05). Conclusion: Liquid smoke coconut shell applied for 5 days increase the amount of collagen in traumatic ulcers in diabetic patients.


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