scholarly journals Hematology of Nile tilapia (Oreochromis niloticus) subjected to anesthesia and anticoagulation protocols

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4237
Author(s):  
Nadia Cristine Weinert ◽  
Julieta Volpato ◽  
Ádson Costa ◽  
Rozyanne Rosa Antunes ◽  
Aldo Camargo de Oliveira ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Hematological Parameters ◽  
Prognostic Indicator ◽  
Control Group ◽  
Average Weight ◽  
Commercial Fish ◽  
Pathological Conditions ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Apparently Healthy

Clinical hematology facilitates the diagnosis of disease and can act as a prognostic indicator of pathological conditions in fish. The aim of the present study was to evaluate hematological parameters of Nile tilapia (Oreochromis niloticus) subjected to different anesthetics and anticoagulants. Thirty apparently healthy fishes (average weight of 473 ± 35. 50 g and mean total length of 29. 33 ± 0. 37 cm), were selected from the local commercial fish farm in the Lages municipality (Santa Catarina, Brazil). The animals were randomly divided into three groups of 10. In two groups, anesthesia was induced with eugenol (70 mg·L- 1) (EG) and Benzocaine hydrochloride (100 mg·L-1) (BG), respectively. Anesthesia was not administered to fish of the third group (CG/control group). Blood samples were obtained by venipuncture of the caudal vessels and placed into microtubes containing sodium heparin or Na2EDTA for further analysis. The results were analyzed by Sigma Stat for Windows, the paired t-test for significant differences between anticoagulants of the same group, and analysis of variance followed by the Tukey test for comparison of means between groups (p ? 0. 05). Most of the observed changes in the erythrogram were significantly higher for the anticoagulant heparin and benzocaine group in comparison to the control group. However, the values obtained for the leukogram were significantly higher for all groups subjected to the Na2EDTA anticoagulant, suggesting that heparin may cause cell clumping. The results suggest that the anesthetics under investigation effectively minimizes the effects of stress caused by handling and invasive procedures, and that the anticoagulant heparin causes less hemolysis in comparison to Na2EDTA for Nile tilapia. Thus, the hematological variations attributed to different anesthetic protocols and/or different anticoagulants should be considered for the species Oreochromis niloticus.

scholarly journals Hematology of Nile tilapia (Oreochromis niloticus) subjected to anesthesia and anticoagulation protocols

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4237
Author(s):  
Nadia Cristine Weinert ◽  
Julieta Volpato ◽  
Ádson Costa ◽  
Rozyanne Rosa Antunes ◽  
Aldo Camargo de Oliveira ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Hematological Parameters ◽  
Prognostic Indicator ◽  
Control Group ◽  
Average Weight ◽  
Commercial Fish ◽  
Pathological Conditions ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Apparently Healthy

<p>Clinical hematology facilitates the diagnosis of disease and can act as a prognostic indicator of pathological conditions in fish. The aim of the present study was to evaluate hematological parameters of Nile tilapia (<em>Oreochromis niloticus</em>) subjected to different anesthetics and anticoagulants. Thirty apparently healthy fishes (average weight of 473 ± 35. 50 g and mean total length of 29. 33 ± 0. 37 cm), were selected from the local commercial fish farm in the Lages municipality (Santa Catarina, Brazil). The animals were randomly divided into three groups of 10. In two groups, anesthesia was induced with eugenol (70 mg·L- 1) (EG) and Benzocaine hydrochloride (100 mg·L-1) (BG), respectively. Anesthesia was not administered to fish of the third group (CG/control group). Blood samples were obtained by venipuncture of the caudal vessels and placed into microtubes containing sodium heparin or Na2EDTA for further analysis. The results were analyzed by Sigma Stat for Windows, the paired t-test for significant differences between anticoagulants of the same group, and analysis of variance followed by the Tukey test for comparison of means between groups (p ? 0. 05). Most of the observed changes in the erythrogram were significantly higher for the anticoagulant heparin and benzocaine group in comparison to the control group. However, the values obtained for the leukogram were significantly higher for all groups subjected to the Na2EDTA anticoagulant, suggesting that heparin may cause cell clumping. The results suggest that the anesthetics under investigation effectively minimizes the effects of stress caused by handling and invasive procedures, and that the anticoagulant heparin causes less hemolysis in comparison to Na2EDTA for Nile tilapia. Thus, the hematological variations attributed to different anesthetic protocols and/or different anticoagulants should be considered for the species <em>Oreochromis niloticus. </em></p>

scholarly journals Compensatory growth in Nile tilapia Oreochromis niloticus: feed conversion ratio, size heterogeneity, and proximal composition

2020 ◽  
Vol 48 (3) ◽  
pp. 421-428
Author(s):  
Gaspar Román Poot-López ◽  
Eucario Gasca-Leyva ◽  
Carlos González- Salas ◽  
Sergio Guillen-Hernández ◽  
Roger Domínguez-May
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Compensatory Growth ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Control Group ◽  
Average Weight ◽  
Feed Conversion ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Size Heterogeneity

A feeding test was performed to evaluate compensatory growth in the Nile tilapia (Oreochromis niloticus) and to assess changes in feed conversion ratio (FCR), body condition, and carcass composition associated with the imposed feeding strategy. The experiment was carried out under two different stages, restricted (RS) and compensated (CS), with six weeks long each other. Three hundred juveniles of Nile tilapia (average weight of 16.4 ± 0.2 g) were divided into three treatments and one control. The control group was fed to apparent satiation four times a day in both stages. The RS treatments were based on 100% (RS100), 80% (RS80), and 60% (RS60) of a feeding chart, respectively. In the next six weeks, fishes from the CS treatments (CS100, CS80, and CS60) were then fed to apparent satiation four times a day. In the RS, the control group displayed the most considerable weight gained, but with a significant FCR. Nevertheless, in the CS, the previous restricted treatments (RC80 and RC100) showed a compensatory growth, with a smaller FCR than the control group. The restricted-compensated rations did not have a significant effect on the size heterogeneity; when the food is restricted, fish use to moisturize their tissue to compensate for the muscle loss until they receive more food. However, when the food restriction levels are too high, the weight gain, lipid, and protein levels do not recover as they were before. Thus, restricted (marginally) and compensatory feeding strategies provide evidence that growth rates of fishes can be regulated.

scholarly journals Optimal Replacement of Fish Meal Protein by Stick Water in Diet of Sex-Reversed Nile Tilapia (Oreochromis niloticus)

Animals ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 521
Author(s):  
Uraiwan Wattanakul ◽  
Wattana Wattanakul ◽  
Karun Thongprajukaew
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Fish Meal ◽  
Specific Activity ◽  
Hematological Parameters ◽  
Dietary Treatment ◽  
Normal Liver ◽  
Carcass Quality ◽  
Liver Histopathology ◽  
Nile Tilapia Oreochromis Niloticus

The effects of replacing fish meal (FM) protein with stick water (SW) were investigated during the market stage of sex-reversed Nile tilapia, Oreochromis niloticus (18.49 ± 0.31 g initial body weight). The FM protein was replaced with SW for 10% (10SW), 20% (20SW), 30% (30SW) and 50% (50SW) of the FM. The completely randomized design was conducted in outdoor 15 floating baskets (1.5 × 1.5 × 2 m), comprising three replications with 50 fish each, over an 8 month trial. At the end of the experiment, no differences in survival, growth performance or feed utilization were observed across the dietary treatments (p > 0.05). A significant change in lipase-specific activity was caused by the replacement, without changes to trypsin, chymotrypsin or amylase activities. The fish in all dietary groups exhibited normal liver histopathology, but the fish fed a diet containing SW showed higher numbers of cells accumulating lipids as compared to fish fed the baseline 0SW dietary treatment. Hematological parameters were similar across the five dietary groups. Only fish fed the 20SW diet had superior carcass quality compared to the baseline 0SW group, in terms of crude protein and lipids, but lower or higher replacement levels had negative effects on carcass quality. Findings from the current study support the replacement of FM protein with SW at a level of 20% in the diet of sex-reversed Nile tilapia reared to the market stage. Higher replacement levels might be possible with the supplementation of fatty acids.

scholarly journals EFFECT OF REPLACING MUSTARD OILCAKE BY LINSEED OILCAKE ON GROWTH PERFORMANCE OF NILE TILAPIA (Oreochromis niloticus, Linnaeus, 1758)

2017 ◽  
Vol 22 (1) ◽  
pp. 10-16
Author(s):  
Suraj Babu Ghimire ◽  
Rahul Ranjan
Keyword(s):  
Growth Performance ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Linseed Oil ◽  
Daily Weight Gain ◽  
Average Weight ◽  
Feed Conversion ◽  
Fish Yield ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Significant Difference

 This study was done to explore the effect of replacing mustard oilcake by linseed oilcake in feed on the growth performance of Nile Tilapia (Oreochromis niloticus). Mustard oilcake (MOC) was replaced by linseed oil cake (LOC) at three different level 0%, 50% and 100% to prepare three different types of diets. Diets were prepared with about 18% analyzed crude protein content. Fingerlings were kept in three happa with three replications for each diet in completely randomized design (CRD). Fish of average weight 5.31 g were stocked in happa having size 1.5m×1.5m×1.2m. The experimental fish were fed 3% of their body weight per day, once in the morning for five months continuously. No significant difference (p>0.05) in final mean weight, final total weight, average daily weight gain (DWG), survival rate, extrapolated gross fish yield (GFY), extrapolated net fish yield (NFY) and apparent feed conversion ratio (AFCR) among different treatments were observed. Hence, linseed oilcake can be used as the substitute in the feed of tilapia, if it is locally available.  Journal of Institute of Science and Technology, 2017, 22 (1): 10-16 

scholarly journals Effect of pollution with lead, cupper, cadmium on gene expression pattern of liver GST and serum lysozymes in Nile tilapia (Oreochromis. niloticus)

2020 ◽  
Vol 21 (3) ◽  
pp. 53-60
Author(s):  
Enas Hassan ◽  
Gehad El-Sayed ◽  
Azza Hassan ◽  
Shaimaa Abd Elsalam
Keyword(s):  
Gene Expression ◽  
Uric Acid ◽  
Expression Pattern ◽  
Total Protein ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Gene Expression Pattern ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Nile Tilapia Oreochromis Niloticus

Objective: This study was conducted to evaluate the influence of pollution with lead, copper, cadmium on the gene expression pattern of liver Glutathione-S-transferase and serum lysozyme in Nile tilapia (Oreochromis. niloticus). Design: Descriptive study. Fish: A Total of 120 Nile tilapia fish (Oreochromis niloticus) samples were collected from Lake Manzala, and drainage water at different localities. Procedures: Cd, Cu, and Pb concentrations residues within mid-dorsal muscle tissue, within gills, liver, and kidney were determined. Erythrocyte count, hemoglobin concentration, Packed Cell volume and other blood indices, as well as, total leukocyte count were measured. Biochemically, Alanine transaminase, Aspartate transaminase activities, total protein, creatinine, uric acid, lysozymes activity were estimated. GST gene expression was determined in the liver. Results: The results showed that Pb, Cu and Cd were bio accumulated at a higher level in the liver, kidney and gills of Nile tilapia fish (Oreochromis niloticus) from all sampling sites. The levels of the ALT and AST were increased, total protein and Albumin concentrations were decreased. Creatinine and uric acid were significantly (P≤ 0.05) increased in all groups (compared to the control group. Hematological parameters and lysozyme activity were decreased. Up regulation of the hepatic GST expression levels in Nile tilapia exposed to the heavy metals in comparison to the control value. Conclusion and clinical relevance: This study shows that lead, copper and cadmium were bio accumulated at higher concentration in liver, kidney, gills and muscles of Nile tilapia due to large industrial activities near locations of the sampling sites. Additionally, GST gene expression represents sensitive biomarker of aquatic pollution.

scholarly journals Effect of replacing soybean meal with water melon (Citrullus lanatus) seed meal on the growth, feed utilization and body composition of Nile tilapia (Oreochromis niloticus)

2020 ◽  
Vol 45 (2) ◽  
pp. 59-66
Author(s):  
W. A. Jimoh ◽  
M. O. Shittu ◽  
S. A. Abdulsalami ◽  
F. Y. Okemakin ◽  
A. A. Ayeloja
Keyword(s):  
Weight Gain ◽  
Soybean Meal ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Citrullus Lanatus ◽  
Nutrient Utilization ◽  
Seed Meal ◽  
Average Weight ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Water Melon

The nutritive potential of water melon (Citrullus lanatus) seed meal as dietary protein source in the diet of Nile tilapia (Oreochromis niloticus) using growth performance and nutrient utilization were evaluated in a 56 day feeding trial. One hundred and fifty tilapia fingerlings of average weight 6.12±0.05g were acclimatized for a week, weighed and allotted into five dietary treatments; containing 0, 15, 30, 45 and 60% Citrullus lanatus replacement levels with soybean meal respectively. The diets (35% crude protein and 10% lipid) were isonitrogenous and isolipidic. Each treatment was replicated three times with ten fish per replicate. Fish were fed 5% body weight on two equal proportions per day to determine weight gain, percentage weight gain, specific growth rate, food conversion ratio, protein efficiency ratio and net protein utilization. The result from the study indicated that there was no significant (p>0.05) differences in the FCR and PER between the fish fed control diets and the fish fed test diets.

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