scholarly journals Antimicrobial effect of bothropstoxin-i in broilers

2021 ◽  
Vol 42 (1) ◽  
pp. 267-282
Author(s):  
Ana Carolina Portella Silveira ◽  
◽  
Sarah Natalie Cirilo Gimenes ◽  
Luiz Fernando Barbaresco ◽  
Camila Perdoncini Carvalho ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Pathogenic Bacteria ◽  
Bacterial Resistance ◽  
Microbial Population ◽  
Growth Promotion ◽  
Antimicrobial Effect ◽  
Intestinal Morphology ◽  
Nonprescription Drugs ◽  
Significance Level

Bacterial resistance is a sanitary issue explained by indiscriminate use of nonprescription drugs, and antimicrobial use in food production for growth promotion. Bothropstoxin-I (BthTx-I) is a phospholipase A2 (PLA2) from Bothrops jararacussu venom, which has a known antimicrobial effect. The goal of this study was the unprecedented evaluation of in vivo antimicrobial activity of BthTx-I in broilers. Microbiological, biochemical, and histological parameters were determined using 84 21-day old broilers that were kept in cages with four birds each at a density of 625 cm2/broiler. The experiment was randomized by three treatments with seven repetitions of four broilers each that lasted seven days. The treatments were: 1) bacitracin zinc diet; 2) PLA2-BthTx-I; 3) without additives. The data obtained from the studied variables was subjected to analysis of variance and an F-test at the 5% significance level. Averages of each variable in each treatment were compared by Tukey’s test. Broiler bacterial cloacal counts showed that BthTx-I decreased the microbial population without reducing body weight, intestinal morphology, or liver or kidney histopathological damage. The toxin showed in vivo activity, being an alternative for better performance in the production of broiler chickens, because it acted by decreasing the microbial load of potentially pathogenic bacteria in the intestinal microbiota of the birds and did not cause muscle, liver or kidney damage at the assessed dosage.

scholarly journals Chloramphenicol Resurrected: A Journey from Antibiotic Resistance in Eye Infections to Biofilm and Ocular Microbiota

2019 ◽  
Vol 7 (9) ◽  
pp. 278 ◽  
Author(s):  
Lorenzo
Keyword(s):  
Pathogenic Bacteria ◽  
Bacterial Resistance ◽  
Antimicrobial Agents ◽  
In Vivo Studies ◽  
Antibiofilm Activity ◽  
Eye Infections ◽  
Treatment And Prevention ◽  
Old Drugs

The advent of multidrug resistance among pathogenic bacteria is devastating the worth of antibiotics and changing the way of their administration, as well as the approach to use new or old drugs. The crisis of antimicrobial resistance is also due to the unavailability of newer drugs, attributable to exigent regulatory requirements and reduced financial inducements. The emerging resistance to antibiotics worldwide has led to renewed interest in old drugs that have fallen into disuse because of toxic side effects. Thus, comprehensive efforts are needed to minimize the pace of resistance by studying emergent microorganisms and optimize the use of old antimicrobial agents able to maintain their profile of susceptibility. Chloramphenicol is experiencing its renaissance because it is widely used in the treatment and prevention of superficial eye infections due to its broad spectrum of activity and other useful antimicrobial peculiarities, such as the antibiofilm properties. Concerns have been raised in the past for the risk of aplastic anemia when chloramphenicol is given intravenously. Chloramphenicol seems suitable to be used as topical eye formulation for the limited rate of resistance compared to fluoroquinolones, for its scarce induction of bacterial resistance and antibiofilm activity, and for the hypothetical low impact on ocular microbiota disturbance. Further in-vitro and in vivo studies on pharmacodynamics properties of ocular formulation of chloramphenicol, as well as its real impact against biofilm and the ocular microbiota, need to be better addressed in the near future.

scholarly journals Performance, Antimicrobial Effect and Carcass Parameters of Finisher Broilers Given Xylopia aethiopica Dried Fruits (Grains of Selim) as Additive

2014 ◽  
Vol 47 (4) ◽  
pp. 124-130 ◽  
Author(s):  
Jonathan Ogagaoghene Isikwenu ◽  
Ifeanyi Udeh ◽  
Bernard Izuchukwu Oshai ◽  
Theresa Ogheneremu Kekeke
Keyword(s):  
Weight Gain ◽  
Feed Intake ◽  
Broiler Chickens ◽  
Microbial Population ◽  
Carcass Characteristics ◽  
Antimicrobial Effect ◽  
Daily Weight Gain ◽  
Broiler Chicks ◽  
Final Body Weight ◽  
Carcass Parameters

Abstract The effect of graded levels of grains of selim on the performance, gut microbial population and carcass characteristics of finisher broilers was investigated. Two hundred and four (204) 28 days old broiler chicks (Marshal breed) were randomly allotted to four treatments with each treatment having three replicates of 17 chicks each in a completely randomized design. Finely blended grains of selim was administered through drinking water on treatments 2, 3 and 4 at concentrations of 0.5, 0.7 and 0.9 g per litre while chicks on treatment 1 (control) received 1.0 g per 1.5 litre of antibiotics (Gendox). Chicks were fed ad libitum with isonitrogenous and isocaloric diets containing 20% crude protein and 3000 Kcal/kg metabolisable energy for four weeks. Results showed no significant (P > 0.05) differences among treatments in the final body weight, total weight gain, daily weight gain, total feed intake, daily feed intake and feed:gain ratio. There were differences in the microbial population of the gastro-intestinal tract with colony count decreasing as the concentration of grains of selim increases. Carcass characteristics and organ weights were similar (P > 0.05) except for thigh and spleen weights, and large intestine lengths where differences (P < 0.05) exist. Mortality ranged from 8.47% - 13.47% across treatments. The results indicated that grains of selim have antimicrobial and anthelminthic properties, and promote growth in broiler chickens

scholarly journals Determination of Antimicrobial Activity of Different Essential Oils Obtained from Plants on Staphylococcus aureus Strains Isolated from Foods

2020 ◽  
Vol 8 (4) ◽  
pp. 1012-1017 ◽  
Author(s):  
Hacer Aslan Canberi ◽  
Esra Şentürk ◽  
Simge Aktop ◽  
Pınar Şanlıbaba
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Thyme Oil ◽  
Staphyloccocus Aureus ◽  
Hypericum Perforatum L ◽  
Different Strains

Essential oils (EOs) are known for its antimicrobial activity against several pathogenic bacteria. The present work evaluated the antimicrobial activity of 15 different EOs on survival of different strains of different Staphyloccocus aureus strains isolated from traditional cheeses by disc diffusion method. The most antimicrobial activity on the strains was found as oil thyme oil (mean zone diameter 23.203 mm). Clove oil and black seed oil had the highest antimicrobial activity after thyme oil with average zone diameters of 13.698 mm and 11.267 mm respectively. Hypericum perforatum L. oil (mean zone 6.209 mm), ginger oil (mean zone 6.250 mm) and garlic oil (mean zone 6.267 mm) were the lowest antimicrobial activity. New studies about antimicrobial effect of EOs in vivo conditions are recommended.

Protective effect of Lactobacillus reuteri KUB-AC5 against Salmonella Enteritidis challenge in chickens

2019 ◽  
Vol 10 (1) ◽  
pp. 43-54 ◽  
Author(s):  
M. Nakphaichit ◽  
S. Sobanbua ◽  
S. Siemuang ◽  
W. Vongsangnak ◽  
J. Nakayama ◽  
...  
Keyword(s):  
Microbial Diversity ◽  
Broiler Chickens ◽  
Salmonella Enteritidis ◽  
Pathogenic Bacteria ◽  
Lactobacillus Reuteri ◽  
Early Stage ◽  
Negative Control ◽  
Control Group ◽  
High Dose

Poultry is an important high-quality food and protein source for humans. However, chicken is considered a primary source of foodborne diseases, especially Salmonella Enteritidis infection. Reducing Salmonella contamination in live poultry will thus lower the risk to consumers. Our previous studies reported that Lactobacillus reuteri KUB-AC5 can produce a substance with antimicrobial activity against pathogenic bacteria, especially Salmonella. In vivo testing revealed that this strain greatly influenced the ileal microbiota by improving chicken gastrointestinal health and inhibiting certain pathogenic bacteria. However, its activity against Salmonella in chicken is unknown. This study investigated the effects of the probiotic L. reuteri KUB-AC5 at various concentrations against Salmonella and the microbiota status in the gastrointestinal tract of broiler chickens. Four treatments groups were used: negative-control group (no Salmonella challenge), positive-control group (Salmonella challenge), and 5 or 7 log cfu probiotic supplementation to Salmonella-challenged chickens. The resultant microbial diversities at the growing and finisher stages were not significantly different among the groups (P>0.05). However, a high dosage of KUB-AC5 maintained similar microbial diversity in Salmonella-challenged chickens as observed in the non-challenged group in the early stage. The exposure Salmonella can affect the microbial diversity that consequently contributes to the disease progression in chicken. Low and high dosages of KUB-AC5 eliminated S. Enteritidis from the ileum and caecum at 14, 21 and 35 days of age. A high-dose of KUB-AC5 also enhanced Lactobacillaceae levels in the growing stage in both the ileum and caecum and suppressed Enterobacteriaceae levels in the finisher stage on day 35, whereas these effects were not observed in the low dose of KUB-AC5 or control groups. These results support the potential value of high-dose L. reuteri KUB-AC5 supplementation for three days after hatching in preventing Salmonella infection in chickens.

Effects of an organic acid mixture and methionine supplements on intestinal morphology, protein and nucleic acids content, microbial population and performance of broiler chickens

2011 ◽  
Vol 51 (11) ◽  
pp. 1025 ◽  
Author(s):  
A. A. Saki ◽  
S. M. Eftekhari ◽  
P. Zamani ◽  
H. Aliarabi ◽  
M. Abbasinezhad
Keyword(s):  
Nucleic Acids ◽  
Organic Acids ◽  
Organic Acid ◽  
Dna Content ◽  
Broiler Chickens ◽  
Microbial Population ◽  
Intestinal Morphology ◽  
Acid Mixture ◽  
Broiler Chicks ◽  
And Performance

A study was carried out to investigate the effects of dietary supplementation of an organic acids mixture and two methionine supplements on intestinal morphology, protein and nucleic acids content, microbial population and performance of broiler chickens. Six hundred unsexed day-old Ross 308 broiler chicks were used in a factorial arrangement (3 × 2) based on a completely randomised design by four replicates with 25 chicks in each. Experimental diets consisted of: 0% organic acid mixture + DL-methionine (Treatment 1), 0% organic acid mixture + Alimet (Treatment 2), 0.5% organic acid mixture + DL-methionine (Treatment 3), 0.5% organic acid mixture + Alimet (Treatment 4), 1% organic acid mixture + DL-methionine (Treatment 5) and 1% organic acid mixture + Alimet (Treatment 6). A significant increase in jejunum mucosal DNA content was found by using 1% of organic acid mixture compared with those by other levels of organic acids at 21 days of age (P < 0.05). Also Treatments 5 and 6 had a higher mucosal DNA content than other treatments (P < 0.05) in a similar period. While at grower stage, the results have shown that protein content, protein/DNA and protein/RNA ratio by 0.5 and 1% of organic acids mixture significantly increased compared with those in the 0% level (P < 0.05). The addition of 1% level of organic acid mixture significantly increased villus height compared with 0 and 0.5% level of organic acid mixture at 21 days of age (P < 0.05). Highest and lowest villus heights were obtained by Treatments 1 and 6 (P < 0.05). Crypt depth increased by 0.5 and 1% of organic acid at 21 and 42 days of age (P < 0.05). Villus surface was significantly increased by 1% of organic acid mixture compared with that by 0% level at 21 days of age. The use of organic acid levels especially 1% resulted in an increase in lactic acid bacteria and decreased Enterobacteriaceae counts in the ileum of broiler chicken (P < 0.05). Better bodyweight and feed conversion ratio were obtained (P < 0.05) by 0.5 and 1% of organic acids mixture, although no response was found in feed intake and mortality in this respect (P > 0.05). The results of this study have shown improved reactions on intestinal morphometrical parameters and microflora atmosphors, by using 1% organic acid mixture. In contrast no differences were found between Alimet and DL-methionine in all tested parameters.

scholarly journals Hermetia illucens fat affects the gastrointestinal tract selected microbial populations, their activity, and the immune status of broiler chickens

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Bartosz Kierończyk ◽  
Mateusz Rawski ◽  
Zuzanna Mikołajczak ◽  
Natalia Leciejewska ◽  
Damian Józefiak
Keyword(s):  
Gastrointestinal Tract ◽  
Soybean Oil ◽  
Broiler Chickens ◽  
Pathogenic Bacteria ◽  
Microbial Population ◽  
Suppressive Effect ◽  
Cholesterol Concentration ◽  
Hermetia Illucens ◽  
Bacterial Populations ◽  
Liver Health

Abstract The present study investigated the effect of Hermetia illucens larvae (BSFL) fat, derived using supercritical CO2 extraction and added to broiler chickens’ diets as a partial (50%) or total replacement for commonly used soybean oil, on the gastrointestinal tract (GIT) microbial population, its activity, and selected physiological and immune traits. A total of 576 one-dayold female Ross 308 chicks were randomly assigned to 3 dietary treatments with 16 replicates each. The following treatments were applied: SO – 100% soybean oil, BSFL50 – a mixture of BSFL and soybean oils in a 50:50 ratio, and BSFL100 – 100% BSFL fat. Digesta samples from the crop, jejunum and ceca were collected for further analyses, i.e., pH measurements, fluorescent in situ hybridization, and short-chain fatty acid (SCFA) concentrations. Additionally, the selected plasma biochemical parameters and immunological traits were assessed. In general, the implementation of BSFL fat in broilers’ diets resulted in increased proliferation of potentially pathogenic bacterial populations in the crop, such as Enterobacteriaceae, Bacteroides – Prevotella cluster, and Clostridium perfringens. Furthermore, BSFL100 enhanced microbial activity via total SCFA production and lowered the pH in this segment. However, no detrimental effects were observed in terms of other GIT segments, i.e., the jejunal and cecal microecosystems. The strongest impact on reduction of select components of the microbial population in the cecum was observed with the BSFL50 treatment for potentially pathogenic bacteria such as Enterobacteriaceae, Bacteroides – Prevotella cluster, while commensal populations were also limited, i.e., Bacillus spp., C. leptum subgroup, and C. coccoides – Eubacterium rectale cluster. Additionally, BSFL100 reduced the cholesterol concentration in the blood, while both experimental treatments decreased the ALT level. In conclusion, due to the insufficient release of lauric acid from the BSFL fat in the crop, an adverse shift in the microbiota can be noted. However, a positive suppressive effect on the select components of the cecal microbiota, as well as improvement of liver health suggests implying the BSFL fat in broiler nutrition.

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