Polymorphism among some bread wheat (Triticum aestivum) cultivars in Iraq using SDS-PAGE for total seed proteins as a biochemical marker

Author(s):  
Attyaf AlTamimi ◽  
Zeina ALrufaye
2003 ◽  
Vol 16 (1) ◽  
pp. 35 ◽  
Author(s):  
Alicia L. Lamarque ◽  
Renée H. Fortunato

Total seed proteins of 10 Acacia species were examined by SDS–PAGE. The protein patterns showed qualitative and quantitative differences among the taxa analysed. The main protein components of most species examined had MW's in the range of 38.5–49.0 × 103. Subgenus Aculeiferum differed from subg. Acacia in the presence of a high concentration of proteins in the range of 20–24.5 × 103. Hierarchical clustering of the 10 taxa was undertaken, based on Jaccard distances calculated from electrophoretic data. The species grouped in two main clusters, representing the two subgenera of Acacia that occur in America, namely subg. Acacia and subg. Aculeiferum. The taxonomic placement of Acacia emilioana, a species with uncertain sectional affinity within subg. Aculeiferum, is discussed.


Author(s):  
Dragana Obreht ◽  
Ljiljana Vapa ◽  
Sándor Kis ◽  
Mária-Hajos Takács ◽  
Éva-Bányai Stefánovics ◽  
...  

Total seed proteins in two safflower species (Carthamus tinctorius L. and C. lanatus L) have been separated by the SDS-PAGE method. Their molecular masses ranged from 120 to 20 kDa. All C. tinctorius genotypes under study exhibited identical electrophoretic patterns which differed from the pattern exhibited by the wild species C. lanatus in the number and position of protein bands. Differences in protein profiles occurred in regions around 60 kDa, from 43 to 36 kDa and around 30 kDa. Statistically significant differences in seed protein content were found among safflower genotypes from different countries as well as among genotypes from the same country but from different sites. The highest seed protein content was found in a genotype originating from the USA.


2008 ◽  
Vol 53 (1) ◽  
pp. 13-23
Author(s):  
Elizabeta Miskoska-Milevska ◽  
Blagica Dimitrievska ◽  
Koo Poru ◽  
Zoran Popovski

The protein profiles of tomato seeds from sub-species ( subsp. cultum Brezh., subsp. subspontaneum Brezh. and subsp. spontaneum Brezh.) were analyzed using SDS-PAGE technique. Electrophoreograms and denzitograms of total, soluble and non-soluble proteins of 31 different samples have showed quantitative and qualitative differences. Qualitative differences in electrophoregrams of total seed proteins refer to protein fragments in zone A (114 kDa, 83 kDa and 65 kDa) and protein fragment in zone C (17 kDa). Qualitative differences in electrophoregrams of soluble seed proteins refer to protein fragment in zone A (94 kDa). Qualitative differences in electrophoregrams of nonsoluble seed proteins refer to protein fragments with molecular weights of: 210 kDa, 85 kDa, 67 kDa and 26 kDa.


2001 ◽  
Vol 29 (3-4) ◽  
pp. 331-338 ◽  
Author(s):  
I. N. Xynias ◽  
I. A. Zamani ◽  
E. Gouli-Vavdinoudi ◽  
D. G. Roupakias

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Temesgen Godebo ◽  
Fanuel Laekemariam ◽  
Gobeze Loha

AbstractBread wheat (Triticum aestivum L.) is one of the most important cereal crops in Ethiopia. The productivity of wheat is markedly constrained by nutrient depletion and inadequate fertilizer application. The experiment was conducted to study the effect of nitrogen (N) and potassium (K) fertilizer rates on growth, yield, nutrient uptake and use efficiency during 2019 cropping season on Kedida Gamela Woreda, Kembata Tembaro Zone Southern Ethiopia. Factorial combinations of four rates of N (0, 23, 46 and 69 kg Nha−1) and three rates of K2O (0, 30 and 60 kg Nha−1) in the form of urea (46–0-0) and murate of potash (KCl) (0-0-60) respectively, were laid out in a randomized complete block design with three replications. The results showed that most parameters viz yield, yield components, N uptake and use efficiency revealed significant differences (P < 0.05) due to interaction effects of N and K. Fertilizer application at the rate of 46 N and 30 kg K ha−1 resulted in high grain yield of 4392 kg ha− 1 and the lowest 1041 from control. The highest agronomic efficiency of N (52.5) obtained from the application of 46 kg N ha−1. Maximum physiological efficiency of N (86.6 kg kg−1) and use efficiency of K (58.6%) was recorded from the interaction of 46 and 30 kg K ha−1. Hence, it could be concluded that applying 46 and 30 kg K ha−1was resulted in high grain yield and economic return to wheat growing farmers of the area. Yet, in order to draw sound conclusion, repeating the experiment in over seasons and locations is recommended.


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