Evaluation of Anthelmintic Activity (In -vitro) of Boerhaavia diffusa Linn (Punarnava) Root Extract Including Phytochemical Screening

Xanthium indicum is an important medicinal plant traditionally used in Bangladesh as a folkloric treatment. The current study was undertaken to evaluate thrombolytic, anthelmintic, anti-oxidant, cytotoxic properties with phytochemical screening of methanolic extract of X. indicum leaves. The analysis of phytochemical screening confirmed the existence of phytosetrols and diterpenes. In thrombolytic assay, a significant clot lysis was observed at four concentrations of plant extract compare to the positive control streptokinase (30,000 IU, 15,000 IU) and negative control normal saline. The extract revealed potent anthelmintic activity at different concentrations. In anti-oxidant activity evaluation by two potential experiments namely total phenolic content determination and free radical scavenging assay by 2, 2-diphenylpicrylhydrazyl (DPPH), the leaves extract possess good anti-oxidant property. In the brine shrimp lethality bioassay, the crude extract showed potent (LC50 1.3 μg/mL) cytotoxic activity compare to the vincristine sulfate as a positive control (LC50 0.8 μg/mL).

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Investigation of in-vitro anthelmintic activity of ethanolic leaf extract of Boerhavia diffusa (Nyctaginaceae) including pharmacognostical and phytochemical screening

Journal of Pharmacy Research ◽

10.1016/j.jopr.2013.08.009 ◽

2013 ◽

Vol 7 (8) ◽

pp. 774-780 ◽

Cited By ~ 1

Author(s):

Ramasubramania Raja Rajagopal

Keyword(s):

Leaf Extract ◽

Anthelmintic Activity ◽

Phytochemical Screening ◽

Ethanolic Leaf Extract ◽

Boerhavia Diffusa

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Qualitative Phytochemical Screening and In-vitro Anthelmintic Activity of Adhatoda vasica (Acanthaceae)

Research Journal of Pharmacy and Technology ◽

10.5958/0974-360x.2017.00083.x ◽

2017 ◽

Vol 10 (2) ◽

pp. 414

Author(s):

Shabi R. Ruskin ◽

S. Ajina

Keyword(s):

Anthelmintic Activity ◽

Phytochemical Screening ◽

Adhatoda Vasica

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Anthelmintic Activities of Hexacosa-9, 11-dienoic Acid and 3-hydroxyurs-12-en-28-oic Acid Isolated from Spermacoce verticillata

Chemical Science International Journal ◽

10.9734/csji/2019/v28i130131 ◽

2019 ◽

pp. 1-10 ◽

Cited By ~ 1

Author(s):

S. Garba ◽

M. M. Adeyemi ◽

L. K. Musa

Keyword(s):

Fatty Acid ◽

Anthelmintic Activity ◽

Dienoic Acid ◽

Phytochemical Screening ◽

Active Components ◽

Standard Drug ◽

Pentacyclic Triterpenoid ◽

Death Time

The phytochemical screening, anthelmintics, and characterization of Spermacoce verticillata crude ethanolic extracts were carried out using standard methods. The research is aimed at investigating the in-vitro anthelmintic activity of Spermacoce verticillata and Cochlospermum tinctorium plants for possible active components. The results of phytochemical screening showed the presence of Alkaloids, Flavonoids, Tannin, Phenol, Fatty acid and Cardiac glycoside, Terpenoid and Saponin. The result of the anthelmintic activities showed that Spermacoce verticillata have paralysis time ranging from 25.9 -71.6 mins with death times ranging from 39 - 90.1 mins at concentrations range between 25 - 100 mg/cm3 The results of Chromatographic and Spectroscopic Analyses using TLC, CCH, IR, NMR GC-MS of c leads to the Isolation of two compounds namely Hexacosa-9,11-dienoic acid (fatty acid) and 3-hydroxyurs-12-en-28-oic acid (Pentacyclic triterpenoid) with paralysis/death time at concentration of 50 mg/cm3 of 22.5 mins and 36.3 mins for the fatty acid and 13.2 mins and 20.1 mins for the pentacyclic triterpenoid. The results were compared with standard drug (Albendazole) which showed higher activity than the crude extracts but lower than the isolated compounds. The results of this work therefore confirmed the traditional claim of using the plant for the treatment of helminthiasis disease.

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Anthelmintic A-Type Procyanidins and Further Characterization of the Phenolic Composition of a Root Extract from Paullinia pinnata

Molecules ◽

10.3390/molecules25102287 ◽

2020 ◽

Vol 25 (10) ◽

pp. 2287

Author(s):

Verena Spiegler

Keyword(s):

Crude Extract ◽

Model Organism ◽

Anthelmintic Activity ◽

Root Extract ◽

Traditional Use ◽

Root Extracts ◽

Traditional Remedies ◽

Fast Centrifugal Partition Chromatography

Extracts from the roots of Paullinia pinnata L. are used in West Africa as traditional remedies for a variety of diseases including infestations with soil-transmitted helminths. Based on the results of an ethnopharmacological survey in Ghana, an aqueous acetone (70%) extract was investigated for its anthelmintic and phytochemical properties. Partitioning of the crude extract followed by several fractionation steps of the ethyl acetate phase using Sephadex® LH-20, fast centrifugal partition chromatography, RP-18-MPLC and HPLC led to isolation of six oligomeric A-type procyanidins (1 to 6). To determine the anthelmintic activity, the crude extract, fractions and isolated compounds were tested in vitro against the model organism Caenorhabditis elegans. A significantly better activity was observed for the trimeric A-type procyanidin (1) compared to a B-type trimer. However, this effect could not be generalized for the tetrameric procyanidins, for which the type of the interflavan-linkage (4→6 vs. 4→8) had the greatest impact on the bioactivity. Besides the procyanidins, three novel compounds, isofraxidin-7-O-α-l-rhamnopyranosyl-(1″→6′)-β-d-glucopyranoside (17), 4-methoxycatechol-2-O-(5′′-O-vanilloyl-β-apiofuranosyl)-(1′′→2′)-β-glucopyranoside (18) and a 6-(3-methoxy-4-hydroxyphenyl)-hexane-2,4-diol-2-O-hexoside (19) were isolated together with further ten known compounds (7 to 16), mainly coumarins and coumarinolignans. Except for 3-β-d-glucopyranosyloxy-4-methyl-2(5H)-furanone (15), none of the isolated compounds has previously been described for P. pinnata. The anthelmintic activity was attributed to the presence of procyanidins, but not to any of the other compound classes. In summary, the findings rationalize the traditional use of P. pinnata root extracts as anthelmintic remedies.

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Therapeutic efficacy of Boerhaavia diffusa (Linn.) root methanolic extract in attenuating streptozotocin-induced diabetes, diabetes-linked hyperlipidemia and oxidative-stress in rats

Biomedical Research and Therapy ◽

10.15419/bmrat.v6i7.556 ◽

2019 ◽

Vol 6 (7) ◽

pp. 3293-3306 ◽

Cited By ~ 2

Author(s):

Firoz Akhter ◽

Sahir Sultan Alvi ◽

Parvej Ahmad ◽

Danish Iqbal ◽

Bader Mohammed Alshehri ◽

...

Keyword(s):

Oxidative Dna Damage ◽

Methanolic Extract ◽

Fasting Blood Glucose ◽

Lipid Hydroperoxide ◽

Plasma Lipid ◽

Diabetic Rats ◽

Root Extract ◽

Bioactive Fraction ◽

Boerhaavia Diffusa

Introduction: We have previously demonstrated that sequentially extracted methanolic fractions of Boerhaavia diffusa (Linn.) showed marked antioxidant, antidiabetic and oxidative-DNA damage protective properties in vitro. The present study was undertaken to evaluate the beneficial effects of B.diffusa (Linn.) methanolic root extract and its partially purified bioactive fraction on streptozotocin (STZ)-induced hyperglycemia and hyperlipidemia in rats. Methods: The diabetic rats were treated for fourteen weeks either with methanolic extract of B. diffusa root (D-MT1, D-MT2, and D-MT3 : doses of 50, 150, and 300 mg/rat/day, respectively), partially isolated bioactive fraction (DBT: 0.5 mg/rat/day), or glibenclamide (D-GT: 0.5 mg/rat/day). Results: The level of fasting blood glucose (FBG) and glycated hemoglobin (HbA1c) were significantly alleviated in D-MT- and D-BT treated groups after fourteen weeks of administration. Moreover, plasma lipid profile, free fatty acids (FFAs), phospholipids (PLs), HMG-CoA reductase (HMG-R) activity, conjugated diene (CD), lipid hydroperoxide (LOOH), and malondialdehyde (MDA) were also markedly ameliorate d in all treatment groups. In addition, the activity of antioxidant enzymes, e.g., superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (Gred), and glutathione-S-transferase (GST), were also significantly restored by D-MT — and D-BT — treated groups. Furthermore, histologically, all the unseemly features of nephropathy were extensively regressed and normalized by the administration of B. diffusa and its bioactive fraction. Conclusions: Our results demonstrate a strong antidiabetic and hypolipidemic impact of B. diffusa extract an ideal alternative therapeutic agent in the prevention and treatment of diabetes as well as diabetes-linked hyperlipidemia.

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