scholarly journals PHYTOCHEMICAL SCREENING AND ANTHELMINTIC ACTIVITY OF ROOT EXTRACT OF HUGONIA MYSTAX LINN AGAINST PHERETIMA POSTHUMA – AN IN VITRO STUDY

2018 ◽  
Author(s):  
NARENDER BOGGULA
Keyword(s):  
Anthelmintic Activity ◽  
Root Extract ◽  
Phytochemical Screening

scholarly journals Phytochemical screening and in vitro anthelmintic activity of methanol extract of Terminalia citrina leaves

2015 ◽  
Vol 5 ◽  
pp. S166-S168 ◽  
Author(s):  
Narhari Das ◽  
Durajan Goshwami ◽  
Md. Sharif Hasan ◽  
Sheikh Zahir Raihan ◽  
Nirmal Kumar Subedi
Keyword(s):  
Methanol Extract ◽  
Anthelmintic Activity ◽  
Phytochemical Screening

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTHELMINTIC ACTIVITY OF ETHANOLIC EXTRACT OF LEAF RACHIS OF AZADIRACHTA INDICA Linn. (NEEM)

2017 ◽  
Vol 03 (01) ◽  
pp. 8-12
Author(s):  
Gavalapu Venkateswara Rao ◽  
Abhinav kanagarla ◽  
Allamraju Satyakeertana ◽  
Vinukonda Alekhya ◽  
Dammu Saipriya ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Phytochemical Screening

scholarly journals In vitro thrombolytic, anthelmintic, anti-oxidant and cytotoxic activity with phytochemical screening of methanolic extract of Xanthium indicum leaves

2015 ◽  
Vol 10 (4) ◽  
pp. 854 ◽  
Author(s):  
Antara Ghosh ◽  
Sujan Banik ◽  
Md. Ariful Islam
Keyword(s):  
Cytotoxic Activity ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Anthelmintic Activity ◽  
Positive Control ◽  
Clot Lysis ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Anti Oxidant

<p class="Abstract"><em>Xanthium indicum</em> is an important medicinal plant traditionally used in Bangladesh as a folkloric treatment. The current study was undertaken to evaluate thrombolytic, anthelmintic, anti-oxidant, cytotoxic properties with phytochemical screening of methanolic extract of <em>X. indicum</em> leaves. The analysis of phytochemical screening confirmed the existence of phytosetrols and diterpenes. In thrombolytic assay, a significant clot lysis was observed at four concentrations of plant extract compare to the positive control streptokinase (30,000 IU, 15,000 IU) and negative control normal saline. The extract revealed potent anthelmintic activity at different concentrations. In anti-oxidant activity evaluation by two potential experiments namely total phenolic content determination and free radical scavenging assay by 2, 2-diphenylpicrylhydrazyl (DPPH), the leaves extract possess good anti-oxidant property. In the brine shrimp lethality bioassay, the crude extract showed potent (LC<sub>50</sub> 1.3 μg/mL) cytotoxic activity compare to the vincristine sulfate as a positive control (LC<sub>50</sub> 0.8 μg/mL). </p><p> </p>

Anthelmintic Activities of Hexacosa-9, 11-dienoic Acid and 3-hydroxyurs-12-en-28-oic Acid Isolated from Spermacoce verticillata

2019 ◽  
pp. 1-10 ◽  
Author(s):  
S. Garba ◽  
M. M. Adeyemi ◽  
L. K. Musa
Keyword(s):  
Fatty Acid ◽  
Anthelmintic Activity ◽  
Dienoic Acid ◽  
Phytochemical Screening ◽  
Active Components ◽  
Standard Drug ◽  
Pentacyclic Triterpenoid ◽  
Death Time

The phytochemical screening, anthelmintics, and characterization of Spermacoce verticillata crude ethanolic extracts were carried out using standard methods. The research is aimed at investigating the in-vitro anthelmintic activity of Spermacoce verticillata and Cochlospermum tinctorium plants for possible active components. The results of phytochemical screening showed the presence of Alkaloids, Flavonoids, Tannin, Phenol, Fatty acid and Cardiac glycoside, Terpenoid and Saponin. The result of the anthelmintic activities showed that Spermacoce verticillata have paralysis time ranging from 25.9 -71.6 mins with death times ranging from  39 - 90.1 mins at concentrations range between 25 - 100 mg/cm3 The results of Chromatographic and Spectroscopic Analyses using TLC, CCH, IR, NMR GC-MS of c leads to the Isolation of two compounds namely Hexacosa-9,11-dienoic acid (fatty acid) and 3-hydroxyurs-12-en-28-oic acid (Pentacyclic triterpenoid) with paralysis/death time at concentration of 50 mg/cm3  of 22.5 mins and 36.3 mins for the fatty acid and 13.2 mins and 20.1 mins for the pentacyclic triterpenoid. The results were compared with standard drug (Albendazole) which showed higher activity than the crude extracts but lower than the isolated compounds. The results of this work therefore confirmed the traditional claim of using the plant for the treatment of helminthiasis disease.

scholarly journals Anthelmintic A-Type Procyanidins and Further Characterization of the Phenolic Composition of a Root Extract from Paullinia pinnata

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2287
Author(s):  
Verena Spiegler
Keyword(s):  
Crude Extract ◽  
Model Organism ◽  
Anthelmintic Activity ◽  
Root Extract ◽  
Traditional Use ◽  
Root Extracts ◽  
Traditional Remedies ◽  
Fast Centrifugal Partition Chromatography

Extracts from the roots of Paullinia pinnata L. are used in West Africa as traditional remedies for a variety of diseases including infestations with soil-transmitted helminths. Based on the results of an ethnopharmacological survey in Ghana, an aqueous acetone (70%) extract was investigated for its anthelmintic and phytochemical properties. Partitioning of the crude extract followed by several fractionation steps of the ethyl acetate phase using Sephadex® LH-20, fast centrifugal partition chromatography, RP-18-MPLC and HPLC led to isolation of six oligomeric A-type procyanidins (1 to 6). To determine the anthelmintic activity, the crude extract, fractions and isolated compounds were tested in vitro against the model organism Caenorhabditis elegans. A significantly better activity was observed for the trimeric A-type procyanidin (1) compared to a B-type trimer. However, this effect could not be generalized for the tetrameric procyanidins, for which the type of the interflavan-linkage (4→6 vs. 4→8) had the greatest impact on the bioactivity. Besides the procyanidins, three novel compounds, isofraxidin-7-O-α-l-rhamnopyranosyl-(1″→6′)-β-d-glucopyranoside (17), 4-methoxycatechol-2-O-(5′′-O-vanilloyl-β-apiofuranosyl)-(1′′→2′)-β-glucopyranoside (18) and a 6-(3-methoxy-4-hydroxyphenyl)-hexane-2,4-diol-2-O-hexoside (19) were isolated together with further ten known compounds (7 to 16), mainly coumarins and coumarinolignans. Except for 3-β-d-glucopyranosyloxy-4-methyl-2(5H)-furanone (15), none of the isolated compounds has previously been described for P. pinnata. The anthelmintic activity was attributed to the presence of procyanidins, but not to any of the other compound classes. In summary, the findings rationalize the traditional use of P. pinnata root extracts as anthelmintic remedies.

scholarly journals In Vitro Anthelmintic Activity of Ethanol Stem Bark Extract of Albizia ferruginea (Guill. & Perr.) Benth

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Miriam Tagoe ◽  
Yaw Duah Boakye ◽  
Theresah Appiah Agana ◽  
Vivian Etsiapa Boamah ◽  
Christian Agyare
Keyword(s):  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Motility Assay ◽  
Bark Extract ◽  
Phytochemical Screening ◽  
Methanol Fraction ◽  
Test Concentration ◽  
Stem Bark Extract

Albizia ferruginea (Guill. & Perr.) Benth bark is used in the traditional medicine as a vermifuge. This study sought to determine the anthelmintic activity of the stem bark extract of Albizia ferruginea. The powdered A. ferruginea stem bark was extracted with ethanol. Phytochemical screening was carried out on A. ferruginea ethanol extract (AFE) and then screened for its anthelmintic property against Pheretima posthuma and Haemonchus contortus using the adult motility assay. The effect of AFE and its fractions on the anthelminthic activity of mebendazole and albendazole were also determined using the adult worm (P. posthuma) motility assay. AFE showed a dose-dependent anthelmintic activity against P. posthuma and H. contortus. The least concentration of AFE (0.5 mg/mL) paralyzed and killed P. posthuma within 272.50 ± 12.42  min and 354.50 ± 5.06  min of exposure, respectively. AFE at the least test concentration (0.14 mg/mL) caused paralysis and induced death of H. contortus, after at 63.50 ± 2.98 and 254.96 ± 2.44  min of exposure, respectively. AFE extract at 0.25 and 0.125 mg/mL increased the paralytic and helminthicidal activities of albendazole. The paralytic and helminthicidal activities of mebendazole were reduced when combined with AFE (0.25 and 0.125 mg/mL). Among the three fractions obtained from AFE, the methanol fraction showed the highest anthelmintic activity. The methanol fraction at 0.5 mg/mL caused paralysis after 69.90 ± 0.15  min and death of worm after 92.53 ± 0.74  min of exposure. The petroleum ether and ethyl acetate fractions showed relatively low anthelmintic activity. Phytochemical screening of AFE revealed the presence of tannins, saponins, glycosides, alkaloids, and coumarins. The results from this study show that A. ferruginea possesses anthelmintic activity which gives credence to its folkloric use.

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