Evaluation of wound healing potential of pomegranate (Punica granatum) whole fruit extract on skin burn wound in rats (Rattus norvegicus)

The Effect of Treatment Using Stingless Bee Propolis Extract on Burn Wound Healing in Rattus norvegicus Burn would occurs as a result of direct or indirect fire burns, exposure to sunlight and chemicals. This study aims to analyze the effect of stingless bee propolis extract (Tetragonula fuscobalteata) on the burn wound healing of the back skin of Rattus norvegicus. An experimental research was carried out using the control and experimental groups. Twenty five R. norvegicus were divided into 5 groups: the negative control group was given 70% ethanol, the positive control group was given Bioplacenton® and 3 groups were treated with propolis extract with a concentration of 50%, 70% and 100%. Measurement of burn diameter was carried out every 2 days for 21 days. Data were analyzed statistically using the one way Anova method. The results of phytochemical screening showed that the stingless bee propolis extract contained flavonoids, phenolics, tannins and saponins. The test of the effectiveness of propolis extract against burn wound obtained a sig value of 0.00 (< 0.05). stingless bee propolis extract could heal burn wound in R. norvegicus. Luka bakar dapat terjadi akibat terbakar api secara langsung atau tidak langsung, paparan sinar matahari dan bahan kimia. Penelitian ini bertujuan untuk menganalisis pengaruh ekstrak propolis lebah tanpa sengat (Tetragonula fuscobalteata) terhadap penyembuhan luka bakar pada kulit punggung Rattus norvegicus. Metode yang digunakan merupakan penelitian true experimental menggunakan kelompok kontrol dan eksperimen. Dua puluh lima ekor R. norvegicus dibagi menjadi 5 kelompok: kelompok kontrol negatif diberi etanol 70%, kontrol positif diberi Bioplacenton®, dan 3 kelompok perlakuan ekstrak propolis dengan konsentrasi 50%, 70%, dan 100%. Pengukuran diameter luka bakar dilakukan setiap 2 hari selama 21 hari. Data dianalisa secara statistik menggunakan metode Anova satu arah. Hasil skrining fitokimia ekstrak propolis lebah tanpa sengat positif mengandung senyawa flavonoid, fenolik, tanin, dan saponin. Uji efektivitas ekstrak propolis terhadap luka bakar didapatkan nilai sig 0,00 (< 0,05). Ekstrak propolis lebah tanpa sengat dapat menyembuhkan luka bakar pada R. norvegicus.

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Biogenic synthesis of nano-sulfur using Punica granatum fruit peel extract with enhanced antimicrobial activities for accelerating wound healing

Nano Futures ◽

10.1088/2399-1984/ac279b ◽

2021 ◽

Vol 5 (4) ◽

pp. 045003

Author(s):

K Samrat ◽

M N Chandraprabha ◽

R Hari Krishna ◽

R Sharath ◽

B G Harish

Keyword(s):

Wound Healing ◽

Granulation Tissue ◽

Breaking Strength ◽

Punica Granatum ◽

Control Group ◽

Fruit Peel ◽

Burn Wound ◽

Excision Wound ◽

Tissue Weight ◽

Peel Extract

Abstract Microbial wound infections leading to secondary complications in wound healing has resulted in high demand for therapeutic drugs with improved efficacy. Despite achieving enhanced bio-activity and higher bioavailability compared to its bulk form, nano-sulfur (SNP) has been explored to a very limited extent for wound healing applications. In this work, we prepare biogenic SNP (SNP-B) via simple biogenic technique using pomegranate (Punica granatum) peel extract and demonstrate its antimicrobial and wound healing activity. The SNP-B was characterized using powder x-ray diffractometer, FESEM, transmission electron microscopy and Raman spectroscopy. Different wound models (excision, incision, dead space and burn) were used to assess the wound healing potential of SNP-B. The 2% (w/w) SNP-B treated group exhibited enhanced wound contraction rate (excision wound, 99.62 ± 0.59%; burn wound, 99.46 ± 0.59%), breaking strength (393.2 ± 10.87 g cm−2), and granulation tissue weight (166.8 ± 9.45 mg) compared to the control group (excision wound, 84.24 ± 2.78%; burn wound, 90.58 ± 3.2%; breaking strength, 241.3 ± 16.11 g cm−2; granulation tissue weight, 91.17 ± 7.28 mg). The efficacy of 2% (w/w) SNP-B was comparable to that of standard (5% w/w povidone-iodine ointment) in all the wound models analyzed. The SNP-B showed enhanced antibacterial activity with a MIC value of 90, 80, 80, and 60 μg ml−1 for Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Staphylococcus aureus, respectively. The results obtained prove the potential of SNP-B as a multifunctional therapeutic agent for topical applications.

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Design of a biofluid-absorbing bioactive sandwich-structured Zn–Si bioceramic composite wound dressing for hair follicle regeneration and skin burn wound healing

Bioactive Materials ◽

10.1016/j.bioactmat.2020.12.006 ◽

2021 ◽

Vol 6 (7) ◽

pp. 1910-1920

Author(s):

Zhaowenbin Zhang ◽

Wenbo Li ◽

Ying Liu ◽

Zhigang Yang ◽

Lingling Ma ◽

...

Keyword(s):

Wound Healing ◽

Hair Follicle ◽

Wound Dressing ◽

Skin Burn ◽

Burn Wound ◽

Burn Wound Healing

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Effects of Nitric Oxide on Skin Burn Wound Healing

Journal of Burn Care & Research ◽

10.1097/bcr.0b013e3181848119 ◽

2008 ◽

Vol 29 (5) ◽

pp. 804-814 ◽

Cited By ~ 39

Author(s):

Haifeng Zhu ◽

Xiaofei Wei ◽

Ka Bian ◽

Ferid Murad

Keyword(s):

Nitric Oxide ◽

Wound Healing ◽

Skin Burn ◽

Burn Wound ◽

Burn Wound Healing

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Test of Burn Wounds Healing Effects of Collagen From Snakehead Fish (Channa striata) Bone in The Preparation of Cream on Male White Rats (Rattus norvegicus)

Indonesian Journal of Pharmaceutical and Clinical Research ◽

10.32734/idjpcr.v3i1.3977 ◽

2020 ◽

Vol 3 (1) ◽

pp. 62-75

Author(s):

Najwa Miladi Hasri ◽

Nilsya Zebua ◽

Sudewi

Keyword(s):

Wound Healing ◽

Rattus Norvegicus ◽

Homogeneity Test ◽

Burn Wound ◽

Burn Wound Healing ◽

White Rats ◽

Significant Difference ◽

Irritation Test ◽

Channa Striata ◽

Post Hoc

Abstract. The use of snakehead fish (Channa striata) collagen in cream is an alternative toreduce fish wastes. It has been proved that snakehead fish (Channa striata) contain collagen.This research aims to study the burn wound healing effectivity of bone’s collagen fromsnakehead fish (Channa striata) that is formulated into a cream. This study used theexperimental method with the bone of snakehead fish (Channa striata) as a sample. This studyconsisted of 4 (four) such as isolation of collagen from snakehead fish's (Channa striata) bone,characterization of collagen by analyzing moisture, ash, protein, fat and functional groupanalysis with FTIR, formulation, and evaluations of cream such as organoleptic test,homogeneity test, pH measurement, stability test, and irritation test, and burn wound healingtest in male white rats (Rattus norvegicus) and the result is analyzed using SPSS 22.0 Free trialand One Way ANOVA and Post-Hoc Tukey HSD. The results showed that fish collagen couldbe isolated from snakehead fish (Channa striata) and the yield obtained is 33.3%. The results ofcollagen characterization and evaluation test met the collagen standard requirements. Results ofthe burn wound healing test on male white rats (Rattus norvegicus) showed that K1 burn woundrecovered on day 21, K2 on day 12, K3 on day 15, K4 on day 18, and K5 on day 18.Measurement of burn wound diameter on day 21 showed K1 = 1.20 cm, K2 = 0.15 cm, K3 =0.10 cm, K4 = 0.45 cm, K5 = 0.40 cm. The results of the statistical analysis of burn wounddiameter showed a significant difference p = 0,000 (p <0.05) between each group. K3 showedthe reduction in the burn wound diameter is faster and the smallest, it can be concluded that theoptimal dose of snakehead fish bone’s collagen cream is a concentration of 3%.

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