The Effectiveness of Ointment of Patah Tulang Stem’s (Euphorbia Tirucalli) Ethanol Extract for Burn Wound Healing on White Rats (Rattus Norvegicus)

Abstract. The use of snakehead fish (Channa striata) collagen in cream is an alternative toreduce fish wastes. It has been proved that snakehead fish (Channa striata) contain collagen.This research aims to study the burn wound healing effectivity of bone’s collagen fromsnakehead fish (Channa striata) that is formulated into a cream. This study used theexperimental method with the bone of snakehead fish (Channa striata) as a sample. This studyconsisted of 4 (four) such as isolation of collagen from snakehead fish's (Channa striata) bone,characterization of collagen by analyzing moisture, ash, protein, fat and functional groupanalysis with FTIR, formulation, and evaluations of cream such as organoleptic test,homogeneity test, pH measurement, stability test, and irritation test, and burn wound healingtest in male white rats (Rattus norvegicus) and the result is analyzed using SPSS 22.0 Free trialand One Way ANOVA and Post-Hoc Tukey HSD. The results showed that fish collagen couldbe isolated from snakehead fish (Channa striata) and the yield obtained is 33.3%. The results ofcollagen characterization and evaluation test met the collagen standard requirements. Results ofthe burn wound healing test on male white rats (Rattus norvegicus) showed that K1 burn woundrecovered on day 21, K2 on day 12, K3 on day 15, K4 on day 18, and K5 on day 18.Measurement of burn wound diameter on day 21 showed K1 = 1.20 cm, K2 = 0.15 cm, K3 =0.10 cm, K4 = 0.45 cm, K5 = 0.40 cm. The results of the statistical analysis of burn wounddiameter showed a significant difference p = 0,000 (p <0.05) between each group. K3 showedthe reduction in the burn wound diameter is faster and the smallest, it can be concluded that theoptimal dose of snakehead fish bone’s collagen cream is a concentration of 3%.

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PENGARUH PEMBERIAN EKSTRAK PROPOLIS LEBAH TANPA SENGAT PADA PENYEMBUHAN LUKA BAKAR TIKUS PUTIH (Rattus norvegicus)

Jurnal Bioteknologi & Biosains Indonesia (JBBI) ◽

10.29122/jbbi.v8i1.4585 ◽

2021 ◽

Vol 8 (1) ◽

pp. 68-76

Author(s):

Nelky Suriawanto ◽

Evi Setyawati ◽

Narwan

Keyword(s):

Wound Healing ◽

Rattus Norvegicus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Stingless Bee ◽

Control Group ◽

Burn Wound ◽

Propolis Extract ◽

Burn Wound Healing

The Effect of Treatment Using Stingless Bee Propolis Extract on Burn Wound Healing in Rattus norvegicus Burn would occurs as a result of direct or indirect fire burns, exposure to sunlight and chemicals. This study aims to analyze the effect of stingless bee propolis extract (Tetragonula fuscobalteata) on the burn wound healing of the back skin of Rattus norvegicus. An experimental research was carried out using the control and experimental groups. Twenty five R. norvegicus were divided into 5 groups: the negative control group was given 70% ethanol, the positive control group was given Bioplacenton® and 3 groups were treated with propolis extract with a concentration of 50%, 70% and 100%. Measurement of burn diameter was carried out every 2 days for 21 days. Data were analyzed statistically using the one way Anova method. The results of phytochemical screening showed that the stingless bee propolis extract contained flavonoids, phenolics, tannins and saponins. The test of the effectiveness of propolis extract against burn wound obtained a sig value of 0.00 (< 0.05). stingless bee propolis extract could heal burn wound in R. norvegicus. Luka bakar dapat terjadi akibat terbakar api secara langsung atau tidak langsung, paparan sinar matahari dan bahan kimia. Penelitian ini bertujuan untuk menganalisis pengaruh ekstrak propolis lebah tanpa sengat (Tetragonula fuscobalteata) terhadap penyembuhan luka bakar pada kulit punggung Rattus norvegicus. Metode yang digunakan merupakan penelitian true experimental menggunakan kelompok kontrol dan eksperimen. Dua puluh lima ekor R. norvegicus dibagi menjadi 5 kelompok: kelompok kontrol negatif diberi etanol 70%, kontrol positif diberi Bioplacenton®, dan 3 kelompok perlakuan ekstrak propolis dengan konsentrasi 50%, 70%, dan 100%. Pengukuran diameter luka bakar dilakukan setiap 2 hari selama 21 hari. Data dianalisa secara statistik menggunakan metode Anova satu arah. Hasil skrining fitokimia ekstrak propolis lebah tanpa sengat positif mengandung senyawa flavonoid, fenolik, tanin, dan saponin. Uji efektivitas ekstrak propolis terhadap luka bakar didapatkan nilai sig 0,00 (< 0,05). Ekstrak propolis lebah tanpa sengat dapat menyembuhkan luka bakar pada R. norvegicus.

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UJI AKTIVITAS EKSTRAK ETANOL BIJI PINANG (Areca catechu L.) TERHADAP PENYEMBUHAN LUKA BAKAR PADA KULIT PUNGGUNG MENCIT PUTIH JANTAN (Mus musculus)

Jurnal Ilmiah Manuntung ◽

10.51352/jim.v2i2.60 ◽

2017 ◽

Vol 2 (2) ◽

pp. 154

Author(s):

Fitri Handayani ◽

Reksi Sundu ◽

Henriko Nober Karapa

Keyword(s):

Antimicrobial Activity ◽

Wound Healing ◽

Treatment Group ◽

Mus Musculus ◽

Healing Process ◽

Ethanol Extract ◽

Phytochemical Analysis ◽

Burn Wound ◽

Burn Wound Healing ◽

Areca Catechu

Areca seeds contain flavonoid, tannin, and alkaloid which can help the healing process of burn wounds on the back skin of mice. Areca seeds are used for treatment because it has antimicrobial activity. Areca Seeds are one of Indonesian plants which the fruit in tranditional way is used as burn medicine. The objective of this study was to know theeffect of ethanol extract of areca seeds against burn wound healing on the back skin of mice. Fifteen mices were used in this experiment and were divided into 5 groups : vaseline flavum as a control negative, branded ointment as a control positive , ethanol extract of areca seeds in vaseline flavum with consentration 20%, 40 % and 60 %. Each group consisted of 3 mices. The back skin of mice was induced using hot iron size 1×1 cm for 2 seconds then given a treatment for 14 days. The percentage of healing of burns by calculating the surface area of wound. The results of its preliminary phytochemical analysis showed the presence of alkaloids, flavonoids, tannins and saponins. The percentage of burn wound healing of ethanol extract in concentration 20 % was 84,33 %, 40 % was 87,67%; and 60% was 89,67%. Data was analyzed using one-way Analysis of Variance (ANOVA) which showed that data has significant differences (P < 0,05) was 0,000. LSD test showed that each treatment group had siginicant differences with significant value < 0,05.

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The Effect of Age on Pain Sensitivity during Burn Wound Healing in Dairy Calves

10.1055/s-0038-1646777 ◽

2018 ◽

Author(s):

S. Adcock ◽

C. Tucker

Keyword(s):

Wound Healing ◽

Pain Sensitivity ◽

Dairy Calves ◽

Burn Wound ◽

Burn Wound Healing ◽

Effect Of Age

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The Safety and Efficacy of Mupirocin Topical Spray for Burn Wound Healing in A Rat Model

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.10.1.8 ◽

2019 ◽

Vol 10 (01) ◽

Author(s):

Sritharadol Rutthapol ◽

Chunhachaichana Charisopon ◽

Kumlungmak Sukanjana ◽

Buatong Wilaiporn ◽

Dechraksa Janwit ◽

...

Keyword(s):

Wound Healing ◽

Matrix Metalloproteinase ◽

Rat Model ◽

Transforming Growth Factor ◽

Immunohistochemical Study ◽

Enzyme Linked Immunosorbent Assay ◽

Burn Wound ◽

Safety And Efficacy ◽

Burn Wound Healing ◽

Tgf Β1

ABSTRACT This study evaluated the effect of mupirocin topical spray on burn wound healing in a rat model. Fifteen male Sprague Dawley rats were used to create full-thickness burns on the rat dorsum using a cylindrical stainless steel rod. The rats were topically treated with normal saline solution (NSS), mupirocin spray, ointment, and solution. The wound size and morphological evaluation were investigated by photographs and clinical criterions for wound healing. The histology was observed by hematoxylin and eosin (HandE) staining assay. The immunohistochemical study was evaluated by detection of transforming growth factor-beta 1 (TGF-β1), and the ratio of matrix metalloproteinase-9 to the tissue inhibitor of matrix metalloproteinase-1 (MMP-9/TIMP-1) was quantified using the enzyme-linked immunosorbent assay (ELISA) assay. A complete healing was observed at 28 days in all treatments. Mupirocin formulations accelerated the wound healing faster than NSS in size. However, the clinical criteria indicated a desirable skin appearance in the mupirocin spray and ointment treated groups. The histological evaluations showed no differences between the treatments while the immunohistochemical study revealed that all treatments reduced the level of TGF-β1 over time, particularly on day 28 in the mupirocin spray and ointment treated groups. The MMP-9/TIMP-1 ratio was significantly lower in the mupirocin spray and ointment treated groups than in the NSS and mupirocin solution groups. This study shows the safety and efficacy in the use of mupirocin topical spray. The topical mupirocin spray is an alternative suitable for development as a human topical anti-infective and wound protection spray.

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Evaluation of Liver and Kidney Toxicity in Rats Receiving Proteoglycans from Fish Cartilage for the Acceleration of Burn Wound Healing

The Bangkok Medical Journal ◽

10.31524/bkkmedj.2016.02.003 ◽

2016 ◽

Vol 11 (01) ◽

pp. 11-16

Author(s):

Sitthiphon Bunman ◽

Narongpon Dumavibhat ◽

Noppadol Larbcharoensub

Keyword(s):

Wound Healing ◽

Burn Wound ◽

Kidney Toxicity ◽

Burn Wound Healing ◽

Liver And Kidney

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Validity of thermography for measuring burn wound healing potential

Wound Repair and Regeneration ◽

10.1111/wrr.12786 ◽

2019 ◽

Vol 28 (3) ◽

pp. 347-354 ◽

Cited By ~ 2

Author(s):

Michelle E. Carrière ◽

Louise E. M. Haas ◽

Anouk Pijpe ◽

Annebeth Meij‐de Vries ◽

Kim L. M. Gardien ◽

...

Keyword(s):

Wound Healing ◽

Burn Wound ◽

Burn Wound Healing

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556 The Use of a Concentrated Surfactant Based Gel to Promote Burn Wound Healing

Journal of Burn Care & Research ◽

10.1093/jbcr/irab032.206 ◽

2021 ◽

Vol 42 (Supplement_1) ◽

pp. S126-S127

Author(s):

Rebecca Coffey ◽

Rachel Penny

Keyword(s):

Wound Healing ◽

New Product ◽

Ease Of Use ◽

Burn Injuries ◽

Full Thickness ◽

Burn Wound ◽

Burn Wounds ◽

Burn Care ◽

Product Trial ◽

Burn Wound Healing

Abstract Introduction Strategies to remove necrotic tissue from burn wounds include excisional and non-excisional debridement. Alternative treatments could promote burn wound healing while minimizing patient discomfort and the need for surgery. We evaluated the usage of a concentrated surfactant gel (CSG) to promote burn wound healing in those with indeterminate depth and full thickness burn injuries. Methods An IRB approved retrospective study was conducted during a 10-patient new product trial period with enrollment between September and October 2019. Patients included in this study had indeterminate or full thickness burn wounds and were treated with a concentrated surfactant-based gel. Patients with non-burn diagnoses were excluded. Data collected included demographic information, injury descriptors, and additional burn wound characteristics. Results A total of 10 patients were included in this study as part of a new product trial. The subjects were 80% male with an average TBSA of 7.5%. 40% had indeterminate and 60% had full thickness burn wounds. Prior to initiation of the CSG, the burn wounds had been open for an average of 41 days. There were no infections or complications with usage of the CSG. 90% of patients reported less pain than the standard of care topical agents for burns. Average duration of treatment with the CSG until healing was 28 days. After usage of the CSG, no patients required surgery. Conclusions Our findings support the usage of a concentrated surfactant-based gel in patients with burn wounds. Patients reported decreased pain during dressing changes and ease of use compared to the standard topical agent in burn care. It also prevented surgical debridement in those with indeterminate and full thickness burn injuries.

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