Biogenic synthesis of nano-sulfur using Punica granatum fruit peel extract with enhanced antimicrobial activities for accelerating wound healing

Nano Futures ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 045003
Author(s):  
K Samrat ◽  
M N Chandraprabha ◽  
R Hari Krishna ◽  
R Sharath ◽  
B G Harish
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Breaking Strength ◽  
Punica Granatum ◽  
Control Group ◽  
Fruit Peel ◽  
Burn Wound ◽  
Excision Wound ◽  
Tissue Weight ◽  
Peel Extract

Abstract Microbial wound infections leading to secondary complications in wound healing has resulted in high demand for therapeutic drugs with improved efficacy. Despite achieving enhanced bio-activity and higher bioavailability compared to its bulk form, nano-sulfur (SNP) has been explored to a very limited extent for wound healing applications. In this work, we prepare biogenic SNP (SNP-B) via simple biogenic technique using pomegranate (Punica granatum) peel extract and demonstrate its antimicrobial and wound healing activity. The SNP-B was characterized using powder x-ray diffractometer, FESEM, transmission electron microscopy and Raman spectroscopy. Different wound models (excision, incision, dead space and burn) were used to assess the wound healing potential of SNP-B. The 2% (w/w) SNP-B treated group exhibited enhanced wound contraction rate (excision wound, 99.62 ± 0.59%; burn wound, 99.46 ± 0.59%), breaking strength (393.2 ± 10.87 g cm−2), and granulation tissue weight (166.8 ± 9.45 mg) compared to the control group (excision wound, 84.24 ± 2.78%; burn wound, 90.58 ± 3.2%; breaking strength, 241.3 ± 16.11 g cm−2; granulation tissue weight, 91.17 ± 7.28 mg). The efficacy of 2% (w/w) SNP-B was comparable to that of standard (5% w/w povidone-iodine ointment) in all the wound models analyzed. The SNP-B showed enhanced antibacterial activity with a MIC value of 90, 80, 80, and 60 μg ml−1 for Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Staphylococcus aureus, respectively. The results obtained prove the potential of SNP-B as a multifunctional therapeutic agent for topical applications.

scholarly journals Pengaruh Papain Getah Pepaya Terhadap Pembentukan Jaringan Granulasi pada Penyembuhan Luka Bakar Tikus Percobaan

2014 ◽  
Vol 3 (1) ◽  
Author(s):  
Meishinta Fitria ◽  
Deddy Saputra ◽  
Gusti Revilla
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Control Group ◽  
Tissue Formation ◽  
Burn Wound ◽  
Control Group Design ◽  
Granulation Tissue Formation ◽  
Burn Wounds ◽  
Group Design ◽  
Burn Wound Healing

AbstrakLuka bakar merupakan masalah yang serius dalam kesehatan dunia, khususnya di negara berkembang. Di Indonesia belum ada laporan tertulis mengenai jumlah penderita luka bakar dan jumlah angka kematian yang diakibatkannya. Di RSUP Dr. M. Djamil Padang pada tahun 2010 ditemukan 84 kasus luka bakar dengan penyebab sengatan listrik, siraman air panas, kompor, dan minyak panas. Sejumlah studi menunjukkan bahwa tanaman tradisional potensial sebagai agen penyembuhan luka, salah satunya papain getah pepaya (Carica papaya). Penelitian ini bertujuan untuk mengetahui pembentukan jaringan granulasi pada penyembuhan luka bakar tikus percobaan. Jenis penelitian ini adalah penelitian eksperimental dengan rancangan posttest only control group design. Subjek penelitian adalah 10 ekor tikus Wistar jantan yang dibagi menjadi dua kelompok, yaitu kelompok kontrol (K) dan kelompok perlakuan (P). Masing-masing kelompok terdiri dari lima (5) ekor tikus. Plat logam (1,5 cm x 1,5 cm) yang dipanaskan digunakan untuk menghasilkan luka bakar full thickness pada bagian dorsal tikus. Papain getah pepaya diberikan pada kelompok P selama 7 hari. Hasil penelitian ini menunjukkan bahwa pemberian papain getah pepaya tidak memberikan pengaruh yang signifikan (p > 0,05) terhadap pembentukan jaringan granulasi pada penyembuhan luka bakar tikus percobaan. Pada kelompok P didapatkan hasil pembuluh darah 29,26 ± 12,34, fibroblas 26,40 ± 21,94, neutrofil 1,4 ± 0,44, limfosit 1,06 ± 0,13, dan makrofag 1,00 ± 0,00. Kesimpulan penelitian ini adalah papain getah pepaya tidak memberikan pengaruh yang signifikan terhadap pembentukan jaringan granulasi pada penyembuhan luka bakar tikus percobaanKata kunci: papain, jaringan granulasi, luka bakar, penyembuhan lukaAbstractBurn wounds is the serious problem in world health specifically for developing contries. In Indonesia, there is no written report about burn wounds patient and mortality account yet. In 2010, M. Djamil Padang Government Public Hospital found 84 cases of burn wounds with some causes as burn effect from sting of electric, hot water, stove flame and hot oil. Some researches indicate that traditional plant able to be wound healing agent as papaya sap. The purposed of this studi was to find out the effect of papain from papaya sap to granulation tissue formation on burn wounds healing in rat models. This was experimental research with posttest only control group design. The subjects were ten male Wistar rats divided in to two group (control group K and experimental group P). Every group consist of five rats. Heated metal plat (1,5 cm x 1,5 cm) used to get full thickness burn wound on dorsal rat part. Then, papain of papaya sap was given to group P for seven days. The results showed that papain of papaya sap didn’t have significant effect (p > 0,05) to granulation tissue formation in burn wound healing of rat models. In group P, the research found the vascular 29,26 ± 12,24, fibroblast 26,40 ± 21,94, neutrophil 1,4 ± 0,44, lymphocyte 1,06 ± 0,13, and macrophag 1,00 ± 0,00. The conclusion of this research is that papain of papaya sap didn’t have the significant effect to granulation tissue formation in burn wound healing of rat models.Keywords:papain, granulation tissue, burn, wound healing

scholarly journals EFFECT OF HEALOL OIL ON WOUND HEALING IN WISTAR RATS: A NOVEL STUDY

2018 ◽  
Vol 11 (3) ◽  
pp. 152
Author(s):  
Sujith V ◽  
Poornima G ◽  
Balaji O ◽  
Bairy Kl ◽  
Praveen K ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Chronic Wounds ◽  
Statistical Significance ◽  
Silver Sulfadiazine ◽  
Histopathological Analysis ◽  
Burn Wound ◽  
Excision Wound ◽  
Burn Wounds ◽  
Wound Model

 Objective: Wound healing is a complex process, and various plant extracts have been used to study the effect of medicinal plants on wound healing. Healol oil is used in some tribal areas for ulcer healing and treatment of burn wounds. There are no animal studies done so far using healol oil to find out its role in the treatment of wounds. Hence, the aim of our study is to find the effect of healol oil in excision as well burn wounds in Wistar rats.Methods: Thirty-six Wistar rats were used, 18 rats in excision wound model and remaining in burn wound model. Each model consists of three groups of six rats each. Povidone-iodine was used as a standard control in excision wound model and silver sulfadiazine was used as a standard control in burn wound model. Healol oil was used as test drug in both the models. Period of epithelization and wound contracture rates were analyzed. Histopathological analysis of the skin tissue was done. One-way analysis of variance was used followed by Tukey’s post hoc test for statistical analysis using SPSS version 23 software, P value of <0.05 was taken as statistical significance.Results: Both standard and test groups showed significantly faster wound healing (p<0.001) compared to control rats treated with paraffin wax in both the models. In excision model standard povidone was significantly (p<0.001) better compared to healol oil whereas in burn model, silver sulfadiazine and healol oil showed comparable results with respect to period of epithelization without statistical significance (p>0.05).Conclusion: Wound healing property of healol oil is proved, and further clinical trial can be done to find out the effect of healol oil on chronic wounds due to various etiologies.

scholarly journals Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds

2018 ◽  
Author(s):  
Yasuhiko Kaita ◽  
Takehiko Tarui ◽  
Hideaki Yoshino ◽  
Takeaki Matsuda ◽  
Yoshihiro Yamaguchi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Therapeutic Effect ◽  
Healing Process ◽  
Control Group ◽  
Type I ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing ◽  
Significant Difference ◽  
Regenerative Cells

AbstractThe purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs.Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses.In vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thickness, and neovascularization between the treatment groups and control group. Although there was no significant difference of therapeutic efficacy between fresh ADRC group and frozen ADRC group, frozen ADRCs improved burn wound healing process in dermal regeneration with increased great type I collagen synthesis compared with fresh ADRCs.These findings indicate that frozen ADRCs allow us to apply not only quickly but also for multiple times, and the cryopreserved ADRCs could therefore be useful for the treatment of burn wounds in clinical settings.

scholarly journals Effectivity of topical mangosteen pericarp extract cream on wound healing in Swiss albino mice

2019 ◽  
Vol 92 (2) ◽  
Author(s):  
Hilda Brigitta Sombolayuk ◽  
Khairuddin Djawad ◽  
Siswanto Wahab ◽  
Upik A. Miskad ◽  
Gemini Alam ◽  
...  
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Inflammatory Cells ◽  
Control Group ◽  
Tissue Formation ◽  
Granulation Tissue Formation ◽  
Swiss Albino Mice ◽  
Accelerate Wound Healing ◽  
Acute Wound ◽  
Albino Mice

Wound healing is a complex physiological process consisting of four phases: coagulation, inflammation, proliferation and migration, and remodeling, each with distinct characteristics. Studies have suggested that mangosteen pericarp extract (MPE) may accelerate wound healing. However, the mechanism has not been fully understood. This study aims to evaluate the effect of MPE cream in various concentrations in acute wound healing of albino mice, both histologically and macroscopically. Thirty-two healthy female Swiss albino mice, aged 6-9 weeks, weight 20-30 g, were included in this study. The samples were randomly divided into eight groups each consisting of 4 mice. The first four groups were treated with MPE cream 5%, 10%, and 20%, and no medication (control group), respectively, and were sacrificed after three days. The other four groups received the same application and were sacrificed after 8 days. Wound bed diameter was measured and biopsy from the skin lesion was performed for histopathologic examination. Mann-Whitney test was used to analyze the diameter of the wound bed and histopathological findings of granulation tissue formation, reepithelialization, and inflammation, with P<0.05 considered as significant. MPE cream significantly improved wound healing by increasing granulation tissue formation, and reepithelialization. In addition, MPE cream application was also shown to decrease the number of inflammatory cells, particularly in 5% and 10% concentrations, both in the 3-day and 8-day groups. MPE cream application can accelerate wound healing and thus can be used in acute wound treatment.

Wound healing potential of green synthesized silver nanoparticles prepared from Lansium domesticum fruit peel extract

2015 ◽  
Vol 5 (2) ◽  
pp. 159-164 ◽  
Author(s):  
Shiv Shankar ◽  
Lily Jaiswal ◽  
Rangabhatla Sai Laxmi Aparna ◽  
Rangabhatla GunneswaraS V Prasad ◽  
Govindappa Prem Kumar ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Fruit Peel ◽  
Green Synthesized Silver Nanoparticles ◽  
Lansium Domesticum ◽  
Peel Extract

scholarly journals Effects of extractum cepae, heparin, allantoin gel and silver sulfadiazine on burn wound healing: an experimental study in a rat model

2012 ◽  
Vol 57 (No. 6) ◽  
pp. 287-292 ◽  
Author(s):  
AS Durmus ◽  
M. Yaman ◽  
HN Can
Keyword(s):  
Wound Healing ◽  
Rat Model ◽  
Silver Sulfadiazine ◽  
Control Group ◽  
Albino Rats ◽  
Burn Wound ◽  
Tissue Samples ◽  
Burned Areas ◽  
Positive Effects ◽  
Burn Wound Healing

These experiments were conducted in order to compare the effects of extractum cepae, heparin, allantoin gel (CTBX) and silver sulfadiazine (SSD) cream on burn wound healing in rats. Thirty six adult, female Wistar albino rats were divided into three equal groups. A burn was made on the back of all rats. The burned areas in the first, second and third groups were covered with cold cream (control), SSD skin cream and CTBX twice a day, respectively. Seven and 14 days later, the rats were sacrificed and burned skin tissue samples were collected from the rats for histopathological examinations. Histopatological evaluations on the 7<sup>th</sup> and 14<sup>th</sup> days showed burn healing to be better in the CTBX and SSD groups with respect to the control group. The best burn wound healing was observed in the CTBX group (P &lt; 0.001). Wound healing was significantly different between the groups at days 7 and 14 (P &lt; 0.001). In conclusion, application of CTBX has significant positive effects on the healing of burn wounds in a rat model. &nbsp;

scholarly journals Experimental Evaluation of Ethanolic Extract ofCarapa guianensisL. Leaf for Its Wound Healing Activity Using Three Wound Models

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
B. Shivananda Nayak ◽  
Joel Kanhai ◽  
David Malcolm Milne ◽  
Lexley Pinto Pereira ◽  
William H. Swanston
Keyword(s):  
Wound Healing ◽  
Drinking Water ◽  
Granulation Tissue ◽  
Dead Space ◽  
Breaking Strength ◽  
Test Group ◽  
Ethanolic Extract ◽  
Wound Contraction ◽  
Hydroxyproline Content ◽  
Wound Healing Activity

The leaves ofCarapa guianensishave been used to treat ulcers, skin parasites, and skin problems. The ethanolic extract ofC. guianensisleaf was evaluated for its antibacterial and wound healing activity using excision, incision and dead space wound models in rats. The animals were randomly divided into two groups (n= 6) in all the models. In the excision wound model test group animals were treated topically with the leaf extract (250 mg kg−1body weight) whereas, control animals were treated with petroleum jelly. In the incision and dead space wound models, the test group animals were treated with extract (250 mg kg−1day−1) orally by mixing in drinking water and the control group animals were maintained with plain drinking water. Healing was assessed by the rate of wound contraction, period of epithelialization, skin breaking strength, granulation tissue weight and hydoxyproline content. On Day 15 extract-treated animals exhibited 100% reduction in the wound area when compared to controls (95%) with significant decrease in the epithelialization period. The extract failed to demonstrate antibacterial activity. Skin breaking strength (P< .001), wet (P< .002) and dry (P< .02) granulation tissue and hydroxyproline content (P< .03) were significantly higher in extract treated animals. The increased rate of wound contraction, skin breaking strength and hydroxyproline content supports potential application ofC. guianensisin wound healing.

scholarly journals Uji Aktivitas Ekstrak Pericarp Kulit Buah Manggis Terpurifikasi Dalam Penyembuhan Luka Eksisi

2018 ◽  
Vol 1 (3) ◽  
pp. 134-138
Author(s):  
Dira Dira ◽  
Yanuarista Yanuarista ◽  
Ria Afrianti
Keyword(s):  
Wound Healing ◽  
Repeated Measures ◽  
Collagen Fibers ◽  
General Linear ◽  
Control Group ◽  
Garcinia Mangostana ◽  
Excision Wound ◽  
Group I ◽  
Significant Difference

Alfa mangostin memiliki berbagai macam bioaktivitas dan merupakan major compound dalam eksrak kulit manggis (Garcinia mangostana L.), alfa mangostin memiliki aktivitas sebagai antioksidan, antiinflamasi dan antibakteri sehingga berperan dalam proses penyembuhan luka. Penelitian ini bertujuan untuk mengamati aktivitas ekstrak pericarp kulit buah manggis terpurifikasi yang mengandung > 90% alfa-mangostin dalam penyembuhan luka eksisi pada mencit putih jantan secara in vivo. Paramater yang diukur adalah persentase penyembuhan luka eksisi, waktu epitelisasi dan kerapatan serabut kolagen. Mencit dibagi menjadi dua kelompok, dimana kelompok I merupakan kelompok kontrol negatif (-) yang hanya diberi sediaan suspensi Na CMC 1%, sedangkan kelompok II merupakan kelompok perlakuan yang diberi sediaan suspensi ekstrak dengan konsentrasi 1%. Pada hari ke-5, ke-10 dan ke-15 diukur persentase penyembuhan luka dan diamati waktu epitelisasi serta kerapatan serabut kolagen. Hasil penelitian dari persentase penyembuhan luka dan waktu epitelisasi yang dianalisa dengan uji General Linear Model Repeated Measures memberikan perbedaan secara nyata (p<0,05), sedangkan untuk pengamatan serabut kolagen menunjukkan tidak ada perbedaan secara nyata (p>0,05) dan untuk waktu epitelisasi yang diuji menggunakan uji T Independent Sample memberikan perbedaan secara nyata (p<0,05), maka dapat disimpulkan bahwa ekstrak terpurifikasi dapat memberikan efek dalam penyembuhan luka eksisi pada mencit.   Alfa mangosteen is a major compound of mangosteen extract cortex and  hasvarious bioactivities, such as anti-oxidant, anti-inflammatory and anti-bacterial so that can be used as wound healing. This research aimed to evaluate the activity of Purified Mangosteen Pericarp Fruit Cortex which contained alfa mangosteen > 90% as excision wound healing  agent in male mice by in vivo study. The parameters observed were Excision wound healing, epithelialization time and collagen fiber density. Mice were divided into two groups, group I was the negative control group (-) which was only received 1% Na CMC suspension, while group II was the treatment group received 1 % extract suspension.  On the 5th, 10th and 15th days the percentage of wound healing was measured and the epithelialization time and density of collagen fibers was observed.The results of the percentage of wound healing and epithelialization time evaluation which were analyzed by the General Linear Repeated Measures test showed a significant differences (p <0.05), whereas observation of collagen fibers showed no significant differences (p> 0.05) and analysis of epithelialization time using Independent Sample T test showed a significant difference (p <0.05), it can be concluded that purified extract has excision wounds healing effect in mice.

scholarly journals Effect of Rambutan Fruit Peel Extract on Total Sperm Counts of Wistar Rats With Obesity

Biota ◽  
2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Kerin Victoria Sipahutar ◽  
Yudhi Nugraha ◽  
Cut Fauziah
Keyword(s):  
Wistar Rats ◽  
Semen Quality ◽  
Sperm Count ◽  
Control Group ◽  
Antioxidant Compounds ◽  
P Value ◽  
High Fat ◽  
Fruit Peel ◽  
Group I ◽  
Peel Extract

Obesity caused by a high-fat diet leads to an altered reproductive hormonal profile, including impaired semen quality. Antioxidants can overcome these conditions. One of the well-known sources of antioxidants is in the rambutan fruit peel extract. This research aimed to figure the effect of rambutan fruit peel extract towards total sperm count in Wistar rats induced with high-fat feed. The study design used post-test only control group,  subjects were 30 male Wistar rats divided into five groups: Group I (Positive Control) was given high-fat feed, Group II (Treatment Control Group) was given 15mg/kg BW rambutan fruit peel extract, whereas group III, IV, V (Treatment Group) were given an extract of rambutan skin with a dosage of 15, 30, and 60 mg/kg BW, respectively. Treatment was administered for 81 days. This study showed that rambutan fruit peel extract with doses of 15, 30, and 60 mg/kg BW has significantly increased total sperm count in Wistar rats induced with high-fat feed. The outcome using Kruskal Wallis shows a result of p-value 0,010 (CI 95%). Antioxidant compounds found in rambutan fruit peel extract significantly increase total sperm count in Wistar rats induced with high-fat feed, with the highest efficacy on the dose of 15mg/kg BW.   Keywords: Fruit Peel Rambutan Extract; ; ; 

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