scholarly journals Acute and sub-acute oral toxicity assessment of the methanolic extract from leaves of Hibiscus rosa-sinensis L. in mice

2015 ◽  
Vol 4 (1) ◽  
pp. 70 ◽  
Author(s):  
Purobi Nath ◽  
Arun Yadav
Keyword(s):  
Methanolic Extract ◽  
Toxicity Assessment ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Hibiscus Rosa Sinensis

Investigation of antiulcer activity of Pergularia extensa Linn

2020 ◽  
Vol 9 (2) ◽  
pp. 23-26
Author(s):  
Pavani C H
Keyword(s):  
Cardiac Glycosides ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Antiulcer Activity ◽  
Acute Oral Toxicity ◽  
Gastric Lesions ◽  
Oral Toxicity ◽  
Control Groups ◽  
Dose Dependent

This study was based on determination of the antiulcer activity from methanol extract was prepared by using barks of pergularia extensa linn.. Priliminary investigations showed presence of saponins, terpenes, cardiac glycosides, alkaloids and sterols. Based on OECD-423 Guidelines, the pharmacology and acute oral toxicity studies were conducted by using methanolic extract. Ulcer development was prevented by Tannins because of their vasoconstriction effects and due to protein precipitation. Similarly, the Methanolic extract of Pergularia extensa Linn shows triterpenoids and saponins. The phytoconstituents are present in the extract and these could be possible agents which are involved in order to prevent gastric lesions induced by aspirin. When compared to ulcerative control groups, this Pergularia extensa Linn., shows a dose dependent curative ratio. The extracts exhibited an inhibition percentage of 27.18, 45.47 and 61.28 at doses of 100, 200 and 400mg/kg doses respectively. 

scholarly journals ACUTE ORAL TOXICITY ASSESSMENT OF THE ETHANOL EXTRACT OF HOLOTHURIA ATRA IN MICE

2019 ◽  
pp. 150-153
Author(s):  
PANDU SALIM HANAFI ◽  
AJI SUTRISNO ◽  
TUTIK MURNIASIH ◽  
HARIJONO ◽  
MASTERIA YUNOVILSA PUTRA ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Toxicity Test ◽  
Ethanol Extract ◽  
Polar Compounds ◽  
Toxicity Assessment ◽  
Acute Oral Toxicity ◽  
Test Results ◽  
Oral Toxicity ◽  
Ethanol Extracts ◽  
Holothuria Atra

Objective: This study aimed to evaluate the toxicological potential of the ethanol extract of Holothuria atra through the acute oral toxicity – acute toxic class method. Methods: The sample was immersed in ethanol for 72 h at room temperature and repeated 3 times. The extracts were evaporated using a vacuum rotary evaporator. The identification of compounds in the ethanol extract of H. atra was carried out using liquid chromatography–mass spectrometry (LCMS) analysis. The acute toxicity test was examined the effects of treating male mice with the ethanol extract of H. atra at 300 and 2000 mg/kg by oral administration for 14 days. On the past day of the toxicity test, liver of all experimental animals was taken for histopathological testing. Results: LCMS analysis showed that the ethanol extract of H. atra is contained polar compounds (chlorogenic acid, coumaric acid, a glycosaminoglycan, and holothurin) and non-polar compounds (fatty acids). Acute toxicity study was performed at a dose of 300 and 2000 mg/kg for 14 consecutive days. No deaths or behavioral changes were observed during the administration of both doses. Histopathological test results on the liver showed a few changes at doses of 2000 mg/kg. Conclusions: The LD50 is equal to 5000 mg/kg and the ethanol extracts of H. atra can be classified as practically nontoxic. However, further studies are required to proceed to clinical studies in humans.

In vitrocytotoxicity andin vivosub-acute oral toxicity assessment of porphyran reduced gold nanoparticles

2012 ◽  
Vol 94 (7) ◽  
pp. 1357-1367 ◽  
Author(s):  
Vinod Venkatpurwar ◽  
Vishal Mali ◽  
Subhash Bodhankar ◽  
Varsha Pokharkar
Keyword(s):  
Gold Nanoparticles ◽  
Toxicity Assessment ◽  
Acute Oral Toxicity ◽  
Oral Toxicity

In-vitro Antioxidant and Anti-Convulsant activity of methanolic extract of Sarasvata Churna using scPTZ And MES Models

2020 ◽  
Vol 10 ◽  
Author(s):  
Rahul Kaushik ◽  
Jainendra Jain ◽  
Avijit Majumdar
Keyword(s):  
Ascorbic Acid ◽  
Nitrous Oxide ◽  
Sodium Valproate ◽  
Anticonvulsant Activity ◽  
Methanolic Extract ◽  
Total Phenolic ◽  
Toxicity Screening ◽  
Acute Oral Toxicity ◽  
Oral Toxicity

Background: Sarasvata Churna is an Ayurvedic formulation for treatment and management of Epilepsy and other maniac disorders since thousands years. Objective: This study aimed to evaluate the in-vitro antioxidant potential, total phenolics and flavonoids content, acute-oral-toxicity and anticonvulsant activity of Sarasvata churna. Materials & Methods: In-vitro antioxidant activity of methanolic extract of Sarasvata churna against Nitrous oxide, Peroxide, Phosphomolybdenum and Hydroxyl radicals performed using Colorimetry against Ascorbic acid as standard along with estimation of total phenolic and flavonoids content. Acute oral toxicity was evaluated using OECD guidelines. Extract in carboxymethyl cellulose at doses of 50,75,100,125,150 and 200mg/kg was screened for anticonvulsant activity using subcutaneous Pentylenetetrazole and Maximal Electroshock models in Swiss Albino Mice (n=6). Sodium valproate was used as standard. Results: IC50 value of methanolic extract in the Nitrous oxide, Peroxide, and Hydroxyl free radical scavenging assay was found to be 165mg/ml, 32.5mg/ml and 253.9mg/ml respectively as compared to 61.58µg/ml, 333.44µg/ml and 351µg/ml respectively of standard Ascorbic acid. In acute oral toxicity screening, animals did not showed any signs of acute and delayed toxicity even up to a dose of 2000mg/kg. Extract offered a protection of 57.39% and 85.26% in scPTZ model (P<0.0001) and 74% and 96.38% in MES model (P<0.0001) at doses of 50 and 200mg/kg respectively as compared to standard at 95% Confidance interval (ANNOVA, Tukey test) indicating a dose dependent protection. Conclusion: Sarasvata churna’s potentials are comparable with standard antioxidant Ascorbic acid and antiepileptic drug Sodium valproate. This preclinical and toxicity screening data can be beneficial in establishing the scientific basis for the use of Sarasvata churna in management of epilepsy.

scholarly journals Antitumor activity of leaves of Himatanthus drasticus (Mart.) Plumel-Apocynaceae (janaguba) in the treatment of Sarcoma 180 tumor

2010 ◽  
Vol 46 (2) ◽  
pp. 199-203 ◽  
Author(s):  
Eliane Leite de Sousa ◽  
Ana Ruth Sampaio Grangeiro ◽  
Isla Vanessa Gomes Alves Bastos ◽  
Guilherme Carvalho Ribeiro Rodrigues ◽  
Maria Joselice e Silva ◽  
...  
Keyword(s):  
Side Effects ◽  
Antitumor Activity ◽  
Growth Inhibition ◽  
Crude Extract ◽  
Methanolic Extract ◽  
Tumor Growth Inhibition ◽  
Sarcoma 180 ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Low Toxicity

Himatanthus drasticus, also known as janaguba, is used popularly in Brazil's Northeastern region in the treatment of cancer. However, no scientific reports are available. The present study is the first investigation on the antitumor activity of crude methanolic extract from Himatanthus drasticus leaves against Sarcoma 180 tumor and on its side effects including acute oral toxicity. The OECD 423 methodology was used to study acute oral toxicity, and the STOCK methodology to assess antitumor activity. The crude extract showed low toxicity at the tested doses (50, 300 and 2000 mg/kg) administered orally. The histopathological analyses demonstrated alterations in liver lung, spleen and kidney. It also showed activity against Sarcoma 180 tumor in male Swiss albino mice, evidencing tumor growth inhibition of 67.7% and 68% at 300 mg/kg and 400 mg/kg doses, respectively.

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