Propagation Potentials of Genotypes and Different Physiological Ages of Stem Cuttings in Jatropha curcas L.

Nitrogen is an important nutrient for the successful metabolism of plants, but its occurrence in soil is always very limited. This nutrient has a significant role to preserve plants during various stress conditions by altering the production rate of phytochemicals as defense weapons. Hence, this study was carried out to analyze the effect of nitrogen supply on the phytochemical composition and relative water content in Jatropha curcas L. under a simulated drought condition. The selected seedlings were grown from stem cuttings and categorized into different treated three groups along with the control. After the completion of the experiment, the growth pattern and phytochemical production were investigated. An increased malondialdehyde activity (MDA) was reported with a reduction in relative water content (RWC) of the leaf and in the biomass of seedlings under drought stress. A significant decrease in the levels of alkaloids, phenol, flavonoids and tannins with an increase in saponins and terpenoids was also observed in only simulated drought stressed plants. While a significant increase in the levels of total alkaloid, tannins, flavonoids and phenols was observed in those plants where exogenous nitrogen was supplied before the start of drought periods, unlike in treated and control plants. Therefore, it was revealed that application of Nitrogen enabled the plants to possess protective mechanism through the production of phytochemicals that facilitate the cell membrane to reduce the detrimental effects caused by drought stress.

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PERTUMBUHAN STEK HORIZONTAL BATANG JARAK PAGAR (Jatropha curcas L.) YANG DIINTRODUKSI DENGAN PSEUDOMONAD FLUORESEN

EKSAKTA Berkala Ilmiah Bidang MIPA ◽

10.24036/eksakta/vol19-iss1/129 ◽

2018 ◽

Vol 19 (1) ◽

pp. 68-75

Author(s):

Linda Advinda

Keyword(s):

Jatropha Curcas ◽

Indole Acetic Acid ◽

Shoot Length ◽

Raw Material ◽

Fluorescent Pseudomonad ◽

Stem Cuttings ◽

Jatropha Curcas L ◽

Randomized Design ◽

Shoot Number ◽

Number Of Shoots

Jatropha curcas L. is one of the plants that can be developed as raw material of biodiesel. One of the efforts to increase the production of J. curcas is the propagation of stem cuttings horizontally. Efforts to stimulate root growth can be accomplished by adding Plant Growth Regulators (PGRs). PGRs can be obtained naturally or synthetically. Indole acetic acid (IAA) is one of PGRs, which is not only produced by plants but can also be produced by rhizobacteria. One such rhizobacteria is fluorescent pseudomonad. The objectives of the study were to know the response of growth of horizontal stem cuttings after being introduced with fluorescent pseudomonad. This research is an experimental research. Complete Randomized Design with 6 treatments and 4 replications. The treatments were soaking of J. curcas stem cuttings with fluorescent pseudomonad isolate suspension PfPj3 suspension concentration of 0% (without suspension), 5%, 10%, 15%, 20%, and 25%. Observation of the research is the beginning of the shoots, the number of shoots, and the length of the J. curcas stem shoot. The data obtained were analyzed using Anova and DNMRT significant level of 5%. The results showed that horizontal stem cuttings of J. curcas with fluorescent pseudomonad suspension influenced early shoot, shoot number, and shoot length. A 10% fluorescent pseudomonad concentration can cause shoots to appear early, while a concentration of 20% causes the largest number of shoots.

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ROOT MORPHOLOGY, SHOOT GROWTH, ANTIOXIDATIVE RESPONSE AND GAS EXCHANGE OF STEM CUTTINGS OF Jatropha Curcas L. ACCESSIONS AT DIFFERENT NACL SALINITY LEVELS

International Journal of Biology Pharmacy and Allied Sciences ◽

10.31032/ijbpas/2021/10.9.1033 ◽

2021 ◽

Vol 10 (9) ◽

Keyword(s):

Gas Exchange ◽

Root Morphology ◽

Jatropha Curcas ◽

Shoot Growth ◽

Stem Cuttings ◽

Nacl Salinity ◽

Jatropha Curcas L ◽

Antioxidative Response ◽

Salinity Levels

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Pengaruh Komposisi Media dan Sumber Eksplan Terhadap Induksi Kalus, Perkecambahan, dan Pertumbuhan Tunas Embrio Somatik Jarak Pagar

Buletin Tanaman Tembakau Serat & Minyak Industri ◽

10.21082/bultas.v4n2.2012.76-84 ◽

2016 ◽

Vol 4 (2) ◽

pp. 76

Author(s):

Tantri Dyah Ayu Anggraeni ◽

Emy Sulistyowati ◽

Rully Dyah Purwati

Keyword(s):

Somatic Embryogenesis ◽

Somatic Embryo ◽

Jatropha Curcas ◽

Stem Cuttings ◽

Media Composition ◽

Embryo Germination ◽

Embryogenic Calli ◽

Jatropha Curcas L ◽

Somatic Embryo Germination ◽

Somatic Embryogenic

Jarak pagar (Jatropha curcas L.) merupakan tanaman penghasil minyak nabati sebagai bahan baku bio-diesel. Selama ini, kebutuhan bahan tanam diperoleh dari benih dan setek. Teknik mikropropagasi khususnya melalui embriogenesis somatik merupakan alternatif untuk penyediaan bahan tanam dalam jumlah besar dengan waktu relatif lebih singkat. Jenis eksplan, genotipe, dan kondisi fisiologis tanaman donor serta jenis dan kondisi fisik mediummerupakan faktor utama yang mempengaruhi keberhasilan embriogenesis somatik. Penelitian ini dilakukan untuk mengetahui eksplan dan komposisi media yang tepat untuk induksi kalus embriogenesis somatik, perkecambahan embrio somatik dan pertumbuhan tunas hasil embriogenesis somatik. Penelitian dilaksanakan di laboratorium Kultur Jaringan, Balai Penelitian Tanaman Pemanis dan Serat mulai bulan April sampai dengan November 2011, meliputi tiga tahap, yaitu 1) menguji komposisi media untuk induksi kalus embriogenesis somatik antara lain M1=MS+0,5 mg/l BAP+0,5 mg 2,4 D; M2= MS+1 mg/l BAP +0,5 mg/l 2,4 D; M3= MS+0,5 mg/l BAP+0,2 mg/l TDZ, dan M4= MS+1 mg/l BAP+0,2 mg/l TDZ; 2) menguji komposisi media untuk induksi perkecambahan embrio somatik antara lain MK1= MS+0,5 mg/l BAP+0,1 mg/l NAA dan MK2= MS+0,5 mg/l BAP+0,4 mg/l IBA; dan 3) menguji komposisi media untuk pertumbuhan tunas embrio somatik antara lain MP1= MS+0,5 mg/l BAP+0,1 mg/l IBA dan MP2= MS+0,5 mg/l BAP+0,1 mg/l IAA. Bahan tanam yang digunakan adalah genotipe IP-3A dan IP-3M dengan sumber eksplan kotiledon dan daun. Hasil penelitian menunjukkan kombinasi MS+0,5 mg/l BAP+0,2 mg/l TDZ dengan sumber eksplan kotiledon paling sesuai untuk induksi kalus embriogenesis somatik. Genotipe IP-3M memiliki respon yang lebih baik disbanding IP-3A dan stabil dari tahap induksi kalus embriogenis somatik, induksi perkecambahan embrio somatik, dan pertumbuhan tunas embrio somatik. Jatropha (Jatropha curcas L.) is an oil producing plants as source of bio-diesel. Planting materials usually are obtained from seeds and stem-cuttings. Micro-propagation techniques especially through somatic embryo-genesis is an alternative to provide a large number of planting material in a relatively short time. Explant sources, genotype and physicological conditions of donor plants, also composition and physical condition of medium are the main factors affecting the successful of somatic embryogenesis. The study was conducted to determine the most suitable combination of explant and media composition for embryogenic calli induc-tion, somatic embryo germination, and shoots growth derived from somatic embryogenesis. The experiment was conducted in the Tissue Culture Laboratory, of Indonesian Sweetener and Fiber Crops Research Insti-tute from April to November 2011 covering three phases: 1) testing media composition to induce somatic embryogenic calli i.e. M1=MS+0.5 mg/l BAP+0.5 mg 2.4 D; M2 = MS+1 mg/l BAP+0.5 mg/l 2.4 D; M3 = MS+0.5 mg/l BAP+0.2 mg/l TDZ and M4 = MS+1 mg/l BAP+ 0.2 mg/l TDZ; 2) testing media composition to induce somatic embryo germination i.e. MK1 = MS+0.5 mg/l BAP+0.1 mg/l NAA and MK2 = MS+0.5 mg/l BAP+0.4 mg/l IBA; and 3) testing media composition to induce somatic embryo shoot growth i.e. MP1 = MS+0.5 mg/l BAP+0.1 mg/l IBA and MP2= MS+0.5 mg/l BAP+0.1 mg/l IAA. Plant material used are genotype IP-3A and IP-3M with cotyledone and leaf as explant sources. The results showed that combination of MS+0.5 mg/l BAP+0.2 mg/l TDZ and cotyledons as explants source is the most suitable for somatic embryogenic calli. IP-3M genotype showed a better response to IP-3A and stable from induction of somatic embryogenic calli, somatic embryo germination, and somatic embryo shoots growth.

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Pertumbuhan Beberapa Genotipe Jarak Pagar (Jatropha Curcas L.) di Tanah Masam

Buletin Agrohorti ◽

10.29244/agrob.v5i2.16806 ◽

2017 ◽

Vol 5 (2) ◽

pp. 251-263

Author(s):

Indah Retnowati ◽

Memen Surahman

Keyword(s):

Jatropha Curcas ◽

Jatropha Curcas L

Indonesia memiliki banyak tanah masam, tetapi penggunaan masih sangat sedikit karena kandungan nutrisi tanah masam rendah. Sementara itu, jarak pagar merupakan tanaman yang dapat tumbuh di berbagai jenis tanah (termasuk lahan marjinal) dan penggunaannya sebagai bahan baku biodiesel. Oleh karena itu, perlunya penelitian mengenai pertumbuhan jarak pagar pada tanah masam sebagai upaya untuk mengembangkan jarak pagar dan penggunaannya di Indonesia.Untuk memulai upaya, penelitian tentang berbagai genotipe jarak pagar di tanah masam telah dilakukan. Tujuan dari penelitian ini yaitu mempelajari genotipe jarak pagar yang berpotensi untuk tanah masam. Penelitian ini dilakukan di UPTD Pengembangan Teknologi Lahan Kering Singabraja, Kecamatan Tenjo, Bogor, Jawa Barat, dari November 2010-Agustus 201. Penelitian ini menggunakan Rancangan Kelompok Lengkap Teracak (RKLT) dengan satu faktor yaitu genotipe. Hasil penelitian menunjukkan berbagai perbedaan pada pertumbuhan diantara genotipe-genotipe jarak pagar. Secara umum, ada lima genotipe jarak pagar yang terbaik di tanah masam (pH 5.0) yaitu Medan I-5-1, Dompu, IP-2P-3-4-1, Sulawesi, dan Bima M.

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