scholarly journals Grain Quality of Doubled Haploid Lines in Rice (Oryza sativa L.) Produced by Anther Culture

2016 ◽  
Vol 8 (5) ◽  
pp. 184
Author(s):  
M. Fazaa ◽  
A. EL Sabagh ◽  
G. Anis ◽  
I. EL-Rewainy ◽  
C. Barutçular ◽  
...  
Keyword(s):  
Anther Culture ◽  
Doubled Haploid ◽  
Grain Quality ◽  
Oryza Sativa L ◽  
Culture Technique ◽  
Rice Grain ◽  
Mineral Element ◽  
Doubled Haploid Lines ◽  
Important Approach ◽  
Positive Correlations

<p>Genetic devlopment to imrove genotypes with high quality is the most important approach of rice. Thus, anther culture technique is one of straight forward approaches for improvement of rice cultivars with good grain quality. Therefore, this investigation aimed to develop some doubled haploid lines (DH) through anther culture technique and evaluate them along with their five parents including two check varieties for some nutritional characteristics. The results indicated that the three mineral element contents (Zn, Mn and Fe) of rice grain were clearly different among genotypes (DH), which implied that genotypic variations might provide opportunities to select for higher mineral element content. Analysis of variance revealed that the differences among genotypes were significant for all studied traits. Highly significant positive correlations were recognized among the studied characteristics. Accordingly, Rice lines with the high nutritional values will use as donors for this trait in rice breeding programs for exploitation and in hybridization. <strong></strong></p>

scholarly journals The Agronomical Performances of Doubled Haploid Lines of Rice (Oryza sativa L.) Derived from Anther Culture

2016 ◽  
Vol 8 (5) ◽  
pp. 177 ◽  
Author(s):  
M. Fazaa ◽  
A. EL Sabagh ◽  
G. Anis ◽  
I. EL-Rewainy ◽  
C. Barutcular ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Correlation Analysis ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Oryza Sativa L ◽  
Culture Technique ◽  
Panicle Length ◽  
Doubled Haploid Lines ◽  
Wide Variability ◽  
Breeding Programmes

<p>Anther culture technique offers great opportunities to accelerate breeding progress. The current study aimed to develop some good doubled haploid lines (DH) through anther culture technique and to evaluate them along with their five parents including two check varieties for yield and yield related traits. Analysis of variance revealed that varietal differences were signified and wide variability present among the genotypes with respect to all the characteristics studied. Correlation analysis revealed that grain yield was highly positive correlated with panicle length. The information on the inter association among the yield attributing characters showed the nature and extent of relationship with each other. Consequently, this will help in the improvement of different characters along with yield in breeding programmes.</p>

Agronomic Performance of Wheat Doubled-Haploid Lines Derived from Cultivars by Anther Culture

1989 ◽  
Vol 103 (2) ◽  
pp. 101-109 ◽  
Author(s):  
P. S. Baenziger ◽  
D. M. Wesenberg ◽  
V. M. Smail ◽  
W. L. Alexander ◽  
G. W. Schaeffer
Keyword(s):  
Anther Culture ◽  
Doubled Haploid ◽  
Agronomic Performance ◽  
Doubled Haploid Lines

scholarly journals In vitro androgenesis in triticale

2017 ◽  
Vol 1 (1) ◽  
pp. 99-100
Author(s):  
Oksana Ivanovna Zaitseva
Keyword(s):  
Genetic Variation ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Doubled Haploids ◽  
External Factors ◽  
Hexaploid Triticale ◽  
Doubled Haploid Lines ◽  
In Vitro Androgenesis

Abstract The technology for in vitro anther culturing was optimized in hexaploid triticale using combination of external factors that allowed to obtain more than 100 doubled haploid lines. Investigation of genetic variation among anther culture derived doubled haploids of triticale showed the occurrence of heterozygous plants.

scholarly journals Heritabilitas, Karakterisasi, dan Analisis Clustergram Galur-galur Padi Dihaploid Hasil Kultur Antera

2018 ◽  
Vol 46 (2) ◽  
pp. 119 ◽  
Author(s):  
Muhammad Fuad Anshori ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Sintho Wahyuning Ardie ◽  
Willy Bayuardi Suwarno ◽  
...  
Keyword(s):  
Anther Culture ◽  
Doubled Haploid ◽  
Rice Field ◽  
Block Design ◽  
Yield Potential ◽  
Panicle Length ◽  
Grain Number ◽  
Doubled Haploid Lines ◽  
Randomized Complete Block Design ◽  
High Heritability

<p>ABSTRACT<br /><br />Doubled haploid rice formation through anther culture becomes one of the alternatives in the plant breeding. The lines need to be characterized, clustered, and selected. This study aimed at determining heritability, characters, and cluster of doubled haploid lines obtained from anther culture. This experiment was conducted at IPB Rice Field Experiment Station, Darmaga, Bogor from October 2016 until January 2017. The experiment was managed according to randomized complete block design (RCBD) with 3 replications and genotype as the single factor treatment. Genotypes used were 60 genotypes consisting of 56 doubled haploid lines and 4 control varieties. The controls were Ciherang, Inpara 5, Inpari 29, and Inpari 34 Salin Agritan. The results showed that all characters have significant effect and high heritability (above 60%), so they could be used as a selection character. The tested genotypes could be grouped into three main groups. The first group consisted of 11 genotypes, the second group consisted of 44 genotypes, and the third group consisted of 5 genotypes. The main group determinant characters were productive tillers, percentage of filled grain number, plant height, panicle length, and weight of 1,000 grains. The number of filled grain and productive tillers can be used as selection characters together with yield potential.<br /><br />Keywords: filled grain, heatmap, panicle length, productive tillers, yield potential<br /><br /></p>

scholarly journals Molecular Marker-Assisted Selection of Rice Grain Quality on Rice (Oryza sativa L.) Lines Tolerant to Fe Toxicity Stress

2017 ◽  
Vol 7 (3) ◽  
pp. 268-276 ◽  
Author(s):  
Silvia Utami ◽  
Utut Widyastuti ◽  
Dwinita Utami ◽  
Ida Rosdianti ◽  
Puji Lestari
Keyword(s):  
Oryza Sativa ◽  
Molecular Marker ◽  
Marker Assisted Selection ◽  
Grain Quality ◽  
Oryza Sativa L ◽  
Rice Grain ◽  
Fe Toxicity ◽  
Selection Of

scholarly journals Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture

2020 ◽  
Vol 56 (No. 4) ◽  
pp. 150-158
Author(s):  
Osama Zuhair Kanbar ◽  
Csaba Lantos ◽  
Paul Karumba Chege ◽  
Erzsébet Kiss ◽  
János Pauk
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Genetic Material ◽  
Triticum Aestivum L ◽  
Average Value ◽  
Doubled Haploid Lines ◽  
Dh Lines

We investigated the anther culture (AC) efficiency of thirteen F<sub>4</sub> combinations of winter wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic entities, i.e. embryo-like structures (ELS), regenerated-, green-, albino-, and transplanted plantlets. The number of green plantlets per 100 anthers (GP/100A) varied from 0.36 to 24.74 GP/100A with a mean of 8.31 GP/100A. Albino plantlets (AP) occurred in each combination, ranging from 0.20 to 22.80 AP/100A with an average value of 5.59 AP/100A. Between 25–87.76 doubled haploid (DH) plants per 100 acclimatised plantlets (DH/100ADP), depending on the combination, with a mean of 59.74% were recovered. We have found the highest DH production in the combinations Béres/Midas, Kalász/Tacitus, Béres/Pamier, and Premio/5009. This improves remarkably the choice of basic genetic material in subsequent crossing programmes. These observations emphasise the usability and efficiency of in vitro AC in producing a large number of DH lines for breeding and the applied researches of winter wheat. Although albinism was found in each combination, it was mitigated by the in vitro AC application.

Utilisation of anther culture in rice (Oryza sativa L.) breeding in Italy

1994 ◽  
Vol 34 (7) ◽  
pp. 911 ◽  
Author(s):  
AM Callegarin ◽  
K Perfanov ◽  
G Dorotea ◽  
G Baldi
Keyword(s):  
Oryza Sativa ◽  
Anther Culture ◽  
Grain Quality ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Market Demand ◽  
Breeding Methods ◽  
Rice Varieties ◽  
Hybrid Lines

Increasing market demand has led to efforts to select for non-sticky, long-grain rice varieties suited to the Italian environment. The variability of recombinant populations derived from crosses between Italian and USA genotypes was exploited. Together with the conventional breeding methods, in vitro anther culture was used to obtain homozygous lines from crosses more quickly. Moreover, hybrid lines were obtained from crosses otherwise impossible to exploit owing to sterility in the F, generation (namely those resulting from crosses involving semi-dwarf USA varieties). Plant regeneration was obtained from 6 F, hybrids whose parents were chosen for their grain quality or their suitability to the Italian environment. About 5500 plantlets were regenerated. Of these, 1000 were albino, and about 50% of the green ones died after transplanting into soil; 65% of the surviving plants were sterile, so about 1000 progeny were obtained (30-445 for each cross). Progeny with desirable characteristics were evaluated for agronomic traits. On the basis of these results, limits and possibilities of anther-culture breeding in rice are discussed.

scholarly journals Karakter Agronomi Galur Padi Dihaploid Asal Kultur Antera Hasil Persilangan Three Way Cross

2018 ◽  
Vol 46 (1) ◽  
pp. 9
Author(s):  
Mohammad Syafii ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Dan Willy Bayuardi Suwarno
Keyword(s):  
Genetic Variability ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Block Design ◽  
Broad Sense ◽  
Yield Potential ◽  
Broad Sense Heritability ◽  
High Genetic Variability ◽  
Doubled Haploid Lines ◽  
Agronomic Character

<p><em></em><em>Anther culture is useful tool in rice breeding. The technique shortens the time to obtain full homozygous plant in just one generation. Information related to genetic variability among the regenerants is important for further analysis including selection activities. The objective of this study was to analyze agronomic character variability and yield potential among doubled-haploid lines (DH0) derived from anther culture. This study was carried out in Center for Agricultural Biotechnology and Genetic Resources Research and Development (BB Biogen) from October 2016 to February 2017. The experiment was arranged in randomized complete block design (RCBD) with three replications. Forty eight doubled haploid lines derived from anther culture and three check varieties were evaluated. The results showed high genetic variability as well as broad sense heritability (more than 90%) for all variables tested. Several lines showed superior agronomic characters compared to check (Ciherang, Inpari 18, Inpago 10). Anther culture could generate high genetic variability for further selection.<br /><br />Keywords: anther culture, broad sense heritability, haploid technology</em></p>

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