scholarly journals Germination of Pomegranate Seeds under Sarcotesta Extraction Methods and Drying

2017 ◽  
Vol 9 (10) ◽  
pp. 198 ◽  
Author(s):  
Deived U. De Carvalho ◽  
Maria A. Da Cruz ◽  
Elisete A. F. Osipi ◽  
Jethro B. Osipe ◽  
Ronan C. Colombo ◽  
...  
Keyword(s):  
Room Temperature ◽  
Punica Granatum ◽  
Extraction Methods ◽  
Linear Increase ◽  
Drying Process ◽  
Germination Test ◽  
Immersion Period ◽  
Physiological Quality ◽  
Pomegranate Seeds ◽  
Punica Granatum L

Aiming to evaluate the effect of degumming methods and natural drying, as well as, the influence of different periods of seeds in quicklime solution on Punica granatum L. germination, were conducted two experiments. At first, the seeds from ripe fruits were subjected to the following sarcotesta extraction: natural fermentation at room temperature, during 72 hours; immersion in quicklime (CaO), during 24 hours; and non-degumming, taken as control. For the second experiment, the extraction method was carried out by the following mixture: seeds, quicklime, and distilled water; differing the immersion period (5, 10, 15, 20, and 25 hours). In both, the experimental design was completely randomized, performing the water content and germination test. The quicklime method is efficient to degrade and to eliminate the sarcotesta on P. granatum seeds, and the drying process provides a better germination. In addition, there is a linear increase on seed physiological quality when upgrades the immersion period in quicklime solution.

scholarly journals Occurrence of a Fruit Spot Disease of Pomegranates Caused by Colletotrichum gloeosporiodes in the Prefecture of Komotini, Greece

Plant Disease ◽  
2011 ◽  
Vol 95 (7) ◽  
pp. 872-872 ◽  
Author(s):  
T. Thomidis ◽  
E. Exadaktylou
Keyword(s):  
Room Temperature ◽  
Morphological Characteristics ◽  
Punica Granatum ◽  
Lesion Development ◽  
Koch’S Postulates ◽  
Randomized Design ◽  
Fruit Spot ◽  
Koch's Postulates ◽  
Punica Granatum L ◽  
Wounded Area

Pomegranate (Punica granatum L.) is an important crop in Greece. In 2010, fruits of the pomegranate cv. Wonderful, in commercial fields located in the Prefecture of Komotini in eastern Greece, were observed to have symptoms of distinct dark brown spots. The waxy acervuli observed in infected tissue were subepidermal, typically with setae and simple, short, erect conidiophores. Conidia were hyaline, one celled, ovoid to oblong, slightly curved or dumbbell shaped, and 10 to 15 μm long and 5 to 7 μm wide. The pathogen was isolated on acidified potato dextrose agar (PDA) (2.5 ml of 85% lactic acid per liter of nutrient medium) and incubated at 23°C for 7 days. The pathogen was identified as Colletotrichum gloeosporiodes (Penz) Sacc. on the basis of morphological characteristics and internal transcribed spacer (ITS) sequence (100% identity to AJ301912, C. gloeosporioides species complex) (3) by CBS Fungal Biodiversity Centre, Identification Service, Utrecht, the Netherlands, (CG-1 isolate deposited in CBS Collection; Accession No. CBS 129372). Koch's postulates were completed in the laboratory by placing a 40-μl drop of suspension (4 × 105 conidia ml–1 of water) on a wounded area of healthy fruits of cv. Wonderful. Fruits were surface sterilized by dipping in 0.1% chlorine solution and allowed to dry in a laminar flow hood. There were 15 inoculated and 15 control fruits (similarly sprayed with sterile distilled water) in a randomized design. Fruits were covered with perforated polythene bags to maintain a high humidity necessary for infection that were removed 48 h after inoculation and the fruits were maintained at room temperature (23 ± 2°C). Lesion development was recorded daily for each fruit. Koch's postulates were satisfied after reisolating the fungus from inoculated fruit that developed symptoms similar to those observed on fruits collected from fields. To our knowledge, this is the first report of the occurrence of C. gloeosporioides as a causal agent of fruit spot of pomegranates in Greece. Fruit spots caused by C. gloeosporioides have been reported in pomegranate fields of other countries around the world (1,2). References: (1) B. K. M. Lakshmi et al. Trop. Agric. Res. 22:183, 2011. (2) D. S. Patel. Indian Phytopathol. 62:252, 2009. (3) B. S. Weir and P. R. Johnston. Mycotaxon 111:209, 2010.

scholarly journals Comparing the number of Iranian pomegranate genotypes based on morphological and biochemical properties

2021 ◽  
Vol 57 (No. 4) ◽  
pp. 158-165
Author(s):  
Sara Yari ◽  
Seyed Abbas Mirjalili ◽  
Amir Mousavi ◽  
Elahe Poorazizi
Keyword(s):  
Punica Granatum ◽  
Biochemical Properties ◽  
Fruit Weight ◽  
Soluble Solids ◽  
Morphological Properties ◽  
Beneficial Effects ◽  
Breeding Programmes ◽  
Pomegranate Seeds ◽  
Harvest Maturity ◽  
Punica Granatum L

Punica granatum L. is one of the oldest known edible fruits. Numerous chemical compounds have been isolated from pomegranate seeds, juice, and peels, which have beneficial effects on human health. This study aimed to perform the physicochemical and morphological properties of twenty-four pomegranate genotypes from various provinces of Iran. Fifteen fruits of each cultivar are collected at harvest maturity in the normal ripening period for the pomegranate from the Iranian pomegranate genetic collection in Yazd, Iran. Five fruits were randomly harvested from each of four orientations of the tree, and were immediately taken to the laboratory for analysis. Three replicates were maintained for each analysis. The results indicated the highest levels of anthocyanin was observed in S783 and R633, while polyphenols in Q529, the antioxidant capacity in N755 and the total soluble solids levels in R633 and the total acidity levels were found in K477 and E336. On the other hand, the fruit weight (in S948), fruit diameter (in SH1738), crown diameter (in R533), total weight of the seeds (in S948), peel thickness (in S716), peel colour (in S948), and red juice (in S783) are significantly affected by the genotype. At a similarity of 50%, the genotypes were divided into nine sub-clusters including A, B, C, D, E, F, G, H and I. These identified genotypes can be rolled out in future breeding programmes.

scholarly journals PRE-GERMINATIVE TREATMENTS IN POMEGRANATE SEEDS (Punica granatum L.):EFFECT ON PHYSIOLOGICAL QUALITY

2017 ◽  
Vol 39 (spe) ◽  
Author(s):  
JOSEANO GRACILIANO DA SILVA ◽  
KILSON PINHEIRO LOPES ◽  
JERFFESON ARAÚJO CAVALCANTE ◽  
NATALI ALMEIDA EVANGELISTA PEREIRA ◽  
ROBERTA CHAIENE ALMEIDA BARBOSA
Keyword(s):  
Accelerated Aging ◽  
Punica Granatum ◽  
Coarse Sand ◽  
Index Number ◽  
Randomized Design ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality ◽  
Number Of Leaves ◽  
Pomegranate Seeds

ABSTRACT This study aimed to evaluate the efficiency of different removal methods of sarcotesta and their effects on the physiological quality of the seeds. We applied the following treatments: T1 - friction in coarse sand on a sieve; T2 and T3 - immersion in sulfuric acid (98%) for 30 minutes and washing in water, with and without a vernalization for 48 hours in a refrigerator at 4 °C, respectively; T4 and T5 - natural fermentation for 72 hours, with seeds submitted and not submitted to drying in shade for seven days; and T6 - seeds with intact sarcotesta as control. The experiment was carried out in a completely randomized design. For the analysis of the viability and vigor, we used the germination test, germination on first count, germination speed index, accelerated aging, emergence test, emergence speed index, number of leaves, plant height, stem diameter, and root length. The data were submitted to analysis of variance and Tukey test. Seeds submitted to fermentation showed the highest germination and emergence values. However, it was not statistically different from the control, probably due to the sensitivity to desiccation or seed dormancy, which was shown in the accelerated aging test.

scholarly journals Elucidation of Volatiles, Anthocyanins, Antioxidant and Sensory Properties of cv. Caner Pomegranate (Punica granatum L.) Juices Produced from Three Juice Extraction Methods

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1497
Author(s):  
Jurga Budiene ◽  
Gamze Guclu ◽  
Kouame Fulbert Oussou ◽  
Hasim Kelebek ◽  
Serkan Selli
Keyword(s):  
Sensory Quality ◽  
Antioxidant Activities ◽  
Punica Granatum ◽  
Extraction Process ◽  
Extraction Methods ◽  
Juice Extraction ◽  
Phytochemical Profiles ◽  
First Time ◽  
Punica Granatum L

This study deals with the characterization of the phytochemical profiles and antioxidant activities of cv. Caner pomegranate (Punica granatum) juices obtained from three different juice extraction methods including halved pomegranate (HPJ), arils (AJ), and macerated arils (MAJ) extraction for the first time. It was found that the type of the juice extraction process had substantial effects on the volatiles, anthocyanin compositions, and antioxidant activities of the samples. Results showed that the AJ sample (593 mg L−1) had more anthocyanin compounds followed by HPJ (555 mg L−1) and MAJ (408 mg L−1) samples. GC-MS analysis revealed a total of 34 volatile compounds. The highest number of volatiles was found in the MAJ sample (1872 µg L−1); thus, the aril maceration process played an important role in increasing the volatiles as compared to the HPJ (751.8 µg L−1) and AJ (710.7 µg L−1) samples. Sensory analysis showed that the HPJ sample was the most preferred and its general impression was higher as compared to the AJ and MAJ samples. The findings of this study elucidated that the juice extraction technique had a significant influence on the phytochemical profiles, sensory quality, and antioxidant activity of pomegranate juices.

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