Antitumour and Antioxidant Activity of Some Red Sea Seaweeds in Ehrlich Ascites Carcinoma in vivo

2011 ◽  
Vol 66 ◽  
pp. 0367 ◽  
Author(s):  
H. H. Ahmed ◽  
M. M. Hegazi ◽  
H. I. Abd-Alla ◽  
E. F. Eskander ◽  
M. S. Ellithey
Keyword(s):  
Antioxidant Activity ◽  
Red Sea ◽  
Ehrlich Ascites Carcinoma ◽  
Ehrlich Ascites

scholarly journals Spectral Characterization, Antioxidant And Anti-Tumor Activity of Kaempferol- 3-O-Alpha-L-Rhamnoside; A Flavonol Glycoside Isolated From The Ethyl Acetate Extract of Pithecellobium Dulce Leaves

2021 ◽  
Author(s):  
Masuma Akter ◽  
Mahadi Hasan ◽  
Shahnaj Parvin ◽  
Aziz Abdur Rahman ◽  
Ekramul Islam
Keyword(s):  
Antioxidant Activity ◽  
Oxidative Damage ◽  
Morphological Changes ◽  
Radical Scavenging ◽  
Ehrlich Ascites Carcinoma ◽  
Biologically Active ◽  
Ehrlich Ascites ◽  
Isolated Compound ◽  
Anti Tumor Activity

Abstract Background: Pithecellobium dulce (Roxb.), an evergreen medium-sized, spiny tree which have vast nutritional values and widely used in ayurvedic medicines and home remedies. The plant has also been a rich source of biologically active compounds. The present study was designed to isolate pure compound and to know the efficacy as antioxidant as well as its anti-tumor activity on Ehrlich ascites carcinoma cell (EAC). Methods: The isolation of the compound was carried out by column chromatography and structure was revealed by 1H-NMR and 13C NMR. The antioxidant activity was investigated by the scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals as well as the inhibition of oxidative damage of pUC19 plasmid DNA, hemolysis and lipid peroxidation induced by 2, 2’-azobis (2-amidinopropane) dihydrochloride (AAPH) in human erythrocytes. In vivo anti-tumor activity of the compound was also evaluated by determining the viable tumor cell count, hematological profiles of experimental mice along with observing morphological changes of EAC cells by fluorescence microscope. Results: The isolated compound showed strong antioxidant activity in DPPH radical scavenging with IC50 of 14.6μg/ml. It also effectively inhibited AAPH induced oxidation in DNA and human erythrocyte model and lipid per oxidation. In anti-tumor assay, 70.89±6.62% growth of inhibition of EAC was observed as compare to the control mice (p<0.05) at a dose 50mg/kg body weight. Conclusion: The compound may have a great importance as a therapeutic agent in preventing oxidative damage of biomolecules and therapeutic use in chemotherapy.

Antitumour and Antioxidant Activity of Some Red Sea Seaweeds in Ehrlich Ascites Carcinoma in vivo

2011 ◽  
Vol 66 (7-8) ◽  
pp. 367-376 ◽  
Author(s):  
Hanaa H. Ahmed ◽  
Muhammad M. Hegazi ◽  
Howaida I. Abd-Allac ◽  
Emad F. Eskander ◽  
Mona S. Ellithey
Keyword(s):  
Red Sea ◽  
Antioxidant Activities ◽  
Ehrlich Ascites Carcinoma ◽  
Tumour Marker ◽  
Mice Model ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Tumour ◽  
Total Antioxidant ◽  
Algal Extracts

1The antitumour activities of extracts from the Red Sea seaweeds Jania rubens, Sargassum subrepandum, and Ulva lactuca were investigated in an in vivo mice model based on intramuscular injection of Ehrlich ascites tumour cells. In parallel, antioxidant activities were measured. Tumour marker levels, liver biochemical parameters, and hepatic oxidant/antioxidant status were measured to prove the anticancer and antioxidant nature of the algal extracts. Significant decreases in carcinoembryonic antigen (CEA) and α-fetoprotein (AFP) levels, activities of liver enzymes, levels of nitric oxide (NO) and malondialdehyde (MDA), and an increase in total antioxidant capacity (TAC) were recorded in groups treated with the algal extracts. Jania rubens was selected for phytochemical screening of its phytoconstituents. In addition, carotenoids, halides, minerals, lipoidal matters, proteins, and carbohydrates were studied. Furthermore, 7-oxo-cholest-5(6)-en-3-ol () and cholesterol (2) were isolated from the dichloromethane fraction.

Trichosanthes dioica seed lectin inhibits Ehrlich ascites carcinoma cells growth in vivo in mice by inducing G 0 /G 1 cell cycle arrest

2021 ◽  
Author(s):  
Shaikh Shohidul Islam ◽  
Md. Rezaul Karim ◽  
A. K. M. Asaduzzaman ◽  
A. H. M. Khurshid Alam ◽  
Zahid Hayat Mahmud ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Ehrlich Ascites Carcinoma ◽  
Carcinoma Cells ◽  
Cycle Arrest ◽  
Ehrlich Ascites ◽  
Trichosanthes Dioica

scholarly journals Antitumour evaluation of di-(2-ethylhexyl) phthalate (DEHP) isolated from Calotropis gigantea L. flower / Evaluacija antitumorskog djelovanja di-(2-etilheksil)-ftalata (DEHP) izoliranog iz cvjetova Calotropis gigantea L.

2012 ◽  
Vol 62 (4) ◽  
pp. 607-615 ◽  
Author(s):  
Muhammad Rowshanul Habib ◽  
Muhammad Rezaul Karim
Keyword(s):  
Antitumour Activity ◽  
Viable Cell ◽  
Mass Gain ◽  
Ehrlich Ascites Carcinoma ◽  
Differential Count ◽  
Calotropis Gigantea ◽  
Ehrlich Ascites ◽  
Ethylhexyl Phthalate ◽  
Dose Dependent

The objective of the study is to explore the anticancer activity of di-(2-ethylhexyl) phthalate (DEHP) isolated from Calotropis gigantea flower against Ehrlich ascites carcinoma cells (EAC) in Swiss albino mice. The activity of DEHP was evaluated at doses of 10, 20 and 40 mg kg-1 body mass applied intraperitoneally. DEHP showed a significant decrease in viable cell count (p < 0.05), mass gain (due to tumour burden) and elevated the life span of EAC cell bearing mice. Altered hematological profiles such as RBC, hemoglobin, WBC and differential count were reverted to normal levels in DEHP-treated mice. DEHP also brought back altered biochemical parameters (glucose, cholesterol, triglycerides, blood urea, SALP and SGOT) to normal level. Results of this study indicate that DEHP show potent dose dependent antitumour activity against EAC in vivo.

scholarly journals In vitro and in vivo evaluation of antitumor activity of methanolic extract of Argyreia nervosa leaves on Ehrlich ascites carcinoma

2015 ◽  
Vol 10 (2) ◽  
pp. 399
Author(s):  
Bhawna Sharma ◽  
Isha Dhamija ◽  
Sandeep Kumar ◽  
Hema Chaudhary
Keyword(s):  
Antitumor Activity ◽  
Solid Tumor ◽  
Methanolic Extract ◽  
Ehrlich Ascites Carcinoma ◽  
Ehrlich Ascites ◽  
Wide Range ◽  
Antioxidant Parameters ◽  
Argyreia Nervosa

<p>The herb of importance like <em>Argyreia nervosa</em> has shown wide range of pharmacological activities. Its methanolic extract of <em>A. nervosa</em> has been explored against Ehrlich ascites carcinoma (EAC) induced liquid and solid tumor in mice. Liquid and solid tumors were induced by intraperitoneal and subcutaneous transplantation of EAC cells in Balb/C mice. Significant and dose dependant results are observed when the mice are sacrificed on 15<sup>th</sup> day for estimation of tumor proliferation, hematological, biochemical and hepatic antioxidant parameters. Mean survival time (days) was increased to 36.5 from 20.5 extract treated mice. The extract also showed a decrease (p&lt;0.001) in body weight and percentage reduction in tumor volume respectively when it was evaluated in solid tumor induced mice for a period of 30 days.  From the result it was concluded that the extract has as a potent antitumor activity and that is comparable to 5-fluorouracil.</p><p> </p>

THE ENZYMIC FORMATION OF 6-(METHYLMERCAPTO)PURINE RIBONUCLEOSIDE 5′-PHOSPHATE

1966 ◽  
Vol 44 (2) ◽  
pp. 229-245 ◽  
Author(s):  
Ian C. Caldwell ◽  
J. Frank Henderson ◽  
A. R. P. Paterson
Keyword(s):  
Tumor Cells ◽  
Blood Cells ◽  
Intraperitoneal Injection ◽  
Ehrlich Ascites Carcinoma ◽  
Carcinoma Cells ◽  
Ehrlich Ascites ◽  
Mouse Tissues ◽  
Enzymatic Methods

6-(Methylmercapto)purine ribonucleoside (Me6MPR) is efficiently phosphorylated in mouse tissues and in Ehrlich ascites carcinoma cells in vivo; tumor cells in vitro and cell-free extracts of the tumor also phosphorylate this analogue ribonucleoside. The product of this reaction has been identified by chemical and enzymatic methods and by its chromatographic behaviour as Me6MPR 5′-phosphate. The evidence presented in this report indicates that no other major metabolites of Me6MPR are formed.The phosphorylation of Me6MPR by cell-free tumor extracts requires ATP and Mn2+ (or Mg2+), and evidence is presented that the reaction is probably mediated by adenosine kinase.Me-14C-6MPR is rapidly taken up by most mouse tissues following its intraperitoneal injection. Forty minutes after injection of the labeled drug, the highest levels of radioactivity were found in intestine, liver, blood cells, lung, and spleen, in descending order; virtually no radioactivity was found in brain tissue or in blood plasma.

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