scholarly journals Symbiotic properties of Bradyrhizobium sp. (Lupinus) assayed on serradella plants

2014 ◽  
Vol 64 (4) ◽  
pp. 359-365
Author(s):  
Mieczysława Deryło ◽  
Józef Bednara ◽  
Maria Głowacka ◽  
Anna Skorupska
Keyword(s):  
Tube Test ◽  
Laboratory Conditions ◽  
Small Seed ◽  
Bradyrhizobium Sp ◽  
Symbiotic Phenotype ◽  
Symbiotic Properties ◽  
Ornithopus Sativus ◽  
Slow Growing ◽  
Strain Usda

Physiological and symbiotic properties of <i>Bradyrhizobium</i> sp. (<i>Lupinus</i>) nodule isolates were compared to the standard slow-growing <i>Bradyrhizobium</i> sp. (<i>Lupinus</i>) strain USDA 3045. Lupine nodules isolates showed typical characteristics for bradyrhizobial strains and nodulated small seed legume, serradella (<i>Ornithopus sativus</i>), in tube test. We observed a permanent physiological segregation of the effective (Fix') and ineffective (Fix-) symbiotic phenotype for all tested bradyrhizobial strains during the growth of serradella in plant tube test. The ultrastructural differences between Fix* and Fix serradella nodules were observed. Rapid and visible nodulation as well as easy assay of the reduction of acetylene make serradella a convenient system for studies of <i>Bradyrhizobium</i> sp. (<i>Lupinus</i>) strains in laboratory conditions.

scholarly journals THE BIOLOGICAL PROPERTIES OF SOYBEAN NODULE BACTERIA WITH THE DIFFERENT SPEED OF GROWTH

2010 ◽  
Vol 10 ◽  
pp. 50-64
Author(s):  
D.V. Krutуlo
Keyword(s):  
16S Rrna ◽  
Biological Properties ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Soybean Nodule ◽  
Bradyrhizobium Sp ◽  
The One ◽  
Symbiotic Properties ◽  
Slow Growing ◽  
Intensive Growth

The phenotypical and genotypic properties of soybean microsymbionts with the different speed of growth were studied. It was established that the strains with the intensive growth were specific to the host-plant, but had differed by their serological, chemotaxonomical and symbiotic properties. The investigated intensive-growing strains were combined into the one serogroup by their antigenic content. The similarity of fatty acids spectrum was observed for the strains with the intensive growth Bradyrhizobium sp. КВ1-1, slow-growing strains B. japonicum 634b and B. japonicum КС2-3. The quantitative and qualitative differences in monosaccharides contents of EPS of the studied strains were revealed. Presence of xylose (2,7 %) and increased contents of rhamnose (18,4 %) were shown to be different in EPS of strain Bradyrhizobium sp. КВ1- 1 and EPS of strains B. japonicum КС2-3 and B. japonicum 634b. Analysis of the sequences of the 16S rRNA genes allowed attributing the strain with the intensive growth to the Bradyrhizobium genus. Genes consistency analysis of 16S rRNA had allowed to refer the intensivegrowing strains to the Bradyrhizobium genus. It was shown that studied intensive-growing strains had formed the nonspecific symbiosis with cow pea.

scholarly journals Taxonomically Different Co-Microsymbionts of a Relict Legume, Oxytropis popoviana, Have Complementary Sets of Symbiotic Genes and Together Increase the Efficiency of Plant Nodulation

2018 ◽  
Vol 31 (8) ◽  
pp. 833-841 ◽  
Author(s):  
Vera I. Safronova ◽  
Andrey A. Belimov ◽  
Anna L. Sazanova ◽  
Elizaveta R. Chirak ◽  
Alla V. Verkhozina ◽  
...  
Keyword(s):  
Root Nodules ◽  
Housekeeping Genes ◽  
Nodule Formation ◽  
Symbiotic Genes ◽  
Internal Transcribed Spacer Region ◽  
Bradyrhizobium Sp ◽  
The Common ◽  
Different Strains ◽  
Slow Growing ◽  
Fix Genes

Ten rhizobial strains were isolated from root nodules of a relict legume Oxytropis popoviana Peschkova. For identification of the isolates, sequencing of rrs, the internal transcribed spacer region, and housekeeping genes recA, glnII, and rpoB was used. Nine fast-growing isolates were Mesorhizobium-related; eight strains were identified as M. japonicum and one isolate belonged to M. kowhaii. The only slow-growing isolate was identified as a Bradyrhizobium sp. Two strains, M. japonicum Opo-242 and Bradyrhizobium sp. strain Opo-243, were isolated from the same nodule. Symbiotic genes of these isolates were searched throughout the whole-genome sequences. The common nodABC genes and other symbiotic genes required for plant nodulation and nitrogen fixation were present in the isolate Opo-242. Strain Opo-243 did not contain the principal nod, nif, and fix genes; however, five genes (nodP, nodQ, nifL, nolK, and noeL) affecting the specificity of plant-rhizobia interactions but absent in isolate Opo-242 were detected. Strain Opo-243 could not induce nodules but significantly accelerated the root nodule formation after coinoculation with isolate Opo-242. Thus, we demonstrated that taxonomically different strains of the archaic symbiotic system can be co-microsymbionts infecting the same nodule and promoting the nodulation process due to complementary sets of symbiotic genes.

Biological Nitrogen Fixation and Root-Nodule Bacteria (Rhizobium Sp. and Bradyrhizobium Sp.) In Two Rehabilitating Sand Dune Areas Planted With Acacia Spp

1985 ◽  
Vol 33 (5) ◽  
pp. 595 ◽  
Author(s):  
YM Barnet ◽  
PC Catt ◽  
DH Hearne
Keyword(s):  
Nitrogen Fixation ◽  
Biological Nitrogen Fixation ◽  
Sand Dune ◽  
Root Nodule ◽  
Nodule Bacteria ◽  
Root Nodule Bacteria ◽  
Bradyrhizobium Sp ◽  
Biological Nitrogen ◽  
Slow Growing ◽  
Rhizobium Sp

This paper reports a study of biological nitrogen fixation in two sand dune regions of New South Wales where planted Acacia spp. had been used in revegetation programmes. At one location (Bridge Hill Ridge), natural regrowth had produced a complex plant community, and native legumes in addition to the planted acacias were present. The other area (Wanda Beach) was a grossly disturbed site which contained only the planted species. Symbiotic fixation in association with Australian legumes occurred at both locations at rates within the range reported by other authors. Distinct seasonal changes were apparent, with higher activities in the cooler months. The legume association seemed the only source of biologically fixed nitrogen at Bridge Hill Ridge, but at Wanda Beach cyanobacteria in an algal mat also made a contribution. Fast and slow-growing bacterial strains were obtained from root nodules of native legumes at both sites and were classed as Rhizobium sp. and Bradyrhizobium sp., respectively. This division was supported by the pattern of serological affinities of the isolates and by differences in their protein profiles demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Two atypical types of root-nodule bacteria were found at Bridge Hill Ridge: non-nodulating, fast-growing isolates and an abnormally slow-growing Bradyrhizobium sp.

scholarly journals FUNCTIONING OF SYMBIOTIC SYSTEMS OF COWPEA – NODULE BACTERIA

2011 ◽  
Vol 12 ◽  
pp. 46-58
Author(s):  
D.V. Krutуlo
Keyword(s):  
Bradyrhizobium Japonicum ◽  
High Specificity ◽  
Positive Influence ◽  
Nodule Bacteria ◽  
Soil Culture ◽  
Nitrogen Fixation Activity ◽  
Bradyrhizobium Sp ◽  
Slow Growing ◽  
Cowpea Rhizobia ◽  
Significant Positive Influence

The nodule bacteria were isolated from the nodules of cowpea. On the basis of phenotypical properties these rhizobia were referred to slow growing bacteria of Bradyrhizobium genus. Interaction features of cowpea with the nodule bacteria of cowpea (Bradyrhizobium sp. (Vigna)) and soybean (Bradyrhizobium japonicum) on nitrogen-free substrate and in soil culture were studied. It was established that the cowpea rhizobia strains possess high specificity to the host plant, promote symbiotic nitrogen fixation activity in 1,8-2,6 times and increase plants aboveground mass yield in 1,4-3,4 times, in comparison with control. The significant positive influence of the active soybean microsymbiont Bradyrhizobium japonicum 46 on the growth and development of cowpea was shown.

scholarly journals The Bradyrhizobium Sp. LmicA16 Type VI Secretion System is Required for Efficient Nodulation of Lupinus Spp.

2021 ◽  
Author(s):  
Tighilt L. ◽  
Boulila F. ◽  
De Sousa BFS ◽  
Giraud E ◽  
Ruiz-Argüeso T ◽  
...  
Keyword(s):  
Wild Type Strain ◽  
Gene Clusters ◽  
Secretion System ◽  
Type Vi Secretion System ◽  
Free Living ◽  
Type Vi Secretion ◽  
Divergent Gene ◽  
Bradyrhizobium Sp ◽  
Symbiotic Phenotype ◽  
Free Living Conditions

Abstract Many bacteria of the genus Bradyrhizobium are capable of inducing nodules in legumes. In this work, the importance of a type VI secretion system (T6SS) in a symbiotic strain of the genus Bradyrhizobium is described. T6SS of Bradyrhizobium sp. LmicA16 (A16) is necessary for efficient nodulation with Lupinus micranthus and L. angustifolius . A mutant in the gene vgrG, coding for a component of the T6SS nanostructure, induced less nodules and smaller plants than the wild type strain (wt) and was less competitive when co-inoculated with the wt strain. A16 T6SS genes are organized in a 26 kb DNA region in two divergent gene clusters of nine genes each. One of these genes codes for a protein (Tsb1) of unknown function but containing a methyltransferase domain. A tsb1 mutant showed an intermediate symbiotic phenotype regarding vgrG mutant and higher mucoidy and motility than the wt strain in free living conditions. T6SS promoter fusions to the lacZ reporter indicate expression in nodules but not in free living cells grown in different media and conditions. The analysis of nodule structure revealed that the level of nodule colonization was significantly reduced in the mutants with respect to the wt strain.

scholarly journals Revealing the Draft Genome Sequence of Bradyrhizobium sp. Strain USDA 3458, an Effective Symbiotic Diazotroph Isolated from Cowpea (Vigna unguiculata) Genotype IT82E-16

2019 ◽  
Vol 8 (38) ◽  
Author(s):  
Richard Allen White ◽  
Jeffrey S. Norman ◽  
Emily E. Mclachlan ◽  
Joseph P. Dunham ◽  
Aaron Garoutte ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Vigna Unguiculata ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Plant Growth Promoting Bacteria ◽  
Content Type ◽  
Bradyrhizobium Sp ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Strain Usda

Pairing plants with plant growth-promoting bacteria is critical to the future of agriculture. Bradyrhizobium sp. strain USDA 3458 isolated from Vigna unguiculata (cowpea) paired with cowpea genotype IT82E-16 represents a novel combination in arid regions. Here, we report the draft genome sequence of strain USDA 3458.

scholarly journals Elucidation of the Genome of Bradyrhizobium sp. Strain USDA 3456, a Historic Agricultural Diazotroph from Cowpea (Vigna unguiculata)

2019 ◽  
Vol 8 (33) ◽  
Author(s):  
Richard Allen White ◽  
Jeffrey S. Norman ◽  
Emily E. Mclachlan ◽  
Joseph P. Dunham ◽  
Aaron Garoutte ◽  
...  
Keyword(s):  
Vigna Unguiculata ◽  
Agricultural Research ◽  
Draft Genome ◽  
Germplasm Collection ◽  
The United States ◽  
United States Department ◽  
Content Type ◽  
Agricultural Applications ◽  
Bradyrhizobium Sp ◽  
Strain Usda

Bradyrhizobium sp. strain USDA 3456 is a historic strain from the United States Department of Agriculture (USDA) Agricultural Research Service (ARS) National Rhizobium Germplasm Collection isolated from Vigna unguiculata (cowpea) in 1966. Strain USDA 3456 has been utilized in global agricultural applications, including improving soil nitrogen fertility. The draft genome sequence here provides a genetic reference of a novel diazotroph.

Human drug taking under controlled laboratory conditions.

1988 ◽  
Author(s):  
Marian W. Fischman ◽  
Richard W. Foltin ◽  
Joseph V. Brady
Keyword(s):  
Laboratory Conditions

Multiple recycling of paperboard: Paperboard characteristics and maximum number of recycling cycles— Part I: Multiple recycling of corrugated base paper

TAPPI Journal ◽  
2019 ◽  
Vol 18 (11) ◽  
pp. 631-638
Author(s):  
FREDERIC KREPLIN ◽  
HANS-JOACHIM PUTZ ◽  
SAMUEL SCHABEL
Keyword(s):  
Compression Test ◽  
Production Capacity ◽  
Strength Properties ◽  
Paper Properties ◽  
Corrugated Board ◽  
Laboratory Conditions ◽  
Base Paper ◽  
Study Results ◽  
Short Span ◽  
Multiple Recycling

Paper for recycling is an important fiber source for the production of corrugated base paper. The change in production capacity toward more and more packaging papers affects the composition of paper for recycling and influences the paper quality. This research project investigated the influence of the multiple recycling of five different corrugated base papers (kraftliner, neutral sulfite semichemical [NSSC] fluting, corrugating medium, testliner 2, and testliner 3) on suspension and strength properties under laboratory conditions. The corrugated board base papers were repulped in a low consistency pulper and processed into Rapid-Köthen laboratory sheets. The sheets were then recycled up to 15 times in the same process. In each cycle, the suspension and the paper properties were recorded. In particular, the focus was on corrugated board-specific parameters, such as short-span compression test, ring crush test, corrugating medium test, and burst. The study results indicate how multiple recycling under laboratory conditions affects fiber and paper properties.

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