PHYTOCHEMICAL SCREENING AND INSECTICIDAL EFFECT OF AQUEOUS EXTRACTS OF ARGAN LEAVES (ARGANIA SPINOSA) ON PEACH APHID (MYZUS PERSICAE

Author(s):  
Nadjiba Meziou Chebouti
2021 ◽  
Vol 11 (2) ◽  
pp. 101-106
Author(s):  
Lassaad Mdellel ◽  
Ahmed Abdelli ◽  
Khaled Omar ◽  
Waleed El-Bassam ◽  
Mazen Al-Khateeb

This study aimed to determine the percentage infestation and population parameters of the green peach aphid Myzus persicae under laboratory conditions and evaluate the effect of aqueous extracts of three different plants (pot marigold: Calendula officinalis, mint: Mentha viridis and rosemary: Salvia rosmarinus) on the mortality of this aphid. Extracts of these plants were used at three concentrations (C1 = 15%, C2 = 30% and C2 = 45%). Results indicate a percentage infestation of 76.9 ± 9.4%, a mean relative growth rate of 0.062 ± 0.007 and generation time of 11.12 ± 1.42 days. All treatments reduced the numbers of aphids and statistically significantly reduced the number (α < 0.01) recorded after treatment with C1, C2 and C3 of each extract. C. officinalis extract was more effective than those of M. viridis and S. rosmarinus. The highest mortality (69.82 ± 5.23%) and efficacy (61.71 ± 4.46%) were recorded for the C3 of aqueous extract of C. officinalis, whereas the lowest mortality (38.24 ± 2.42%) and efficacy (32.41 ± 1.23%) were recorded for the C1 of extract of M. viridis. The data provided indicate that aqueous extracts of C. officinalis, M. viridis and S. rosmarinus have an insecticidal effect on M. persicae and can be integrated into a pest management strategy to reduce M. persicae abundance on pepper plants.


AGROFOR ◽  
2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Salim LEBBAL ◽  
Aissa LEBBAL ◽  
Azzeddine ZERAIB

The research of new control methods that have low negative effects represent analternative aiming to limit disadvantages of chemical control of pests. This studyaimed to test the effect of 11 aqueous plant extracts belonging to four species(Santolina africana, Juniperus thurifera, Artemisia herba-alba and Pituranthosscoparius), on the larvae of the aphid Aphis craccivora installed on the leaves ofRobinia pseudoacacia under laboratory conditions. The insecticidal effect ofaqueous extracts on the mortality of aphids was evaluated by the introduction ofacacia leaflet into an extract and infestation of them by 10 larvae of the aphid.Later, a counting of dead larvae after 3, 6, 12 and 24 h was performed. In addition,we investigated the effect of the aqueous extracts on the orientation of the aphidsby putting the latter in a position of choice between six leaflets, each treated with adifferent extract. In addition, a phytochemical screening was carried out to detectthe presence of saponins and polyphenols in the extracts studied. Through ourstudy, the extract obtained from S. africana at a concentration of 15% was the mosteffective with a mortality rate higher than 80% after 24 h of artificial infestation.As for the effect of the four extracts on the orientation of the cowpea aphid, theydid not attract the larvae massively. Regarding phytochemical screening,differences in the chemical composition of the analyzed extracts were observed.These differences may explain in part the variation of aphicidal effects of testedplant extracts.


2011 ◽  
Vol 35 (6) ◽  
pp. 1059-1066 ◽  
Author(s):  
Camila Cramer Filgueiras ◽  
Paulo Roberto Silva Farias ◽  
Maria das Graças Cardoso ◽  
José Djair Vendramim ◽  
Edson Marcos Leal Soares Ramos ◽  
...  

Studies with botanical insecticides have become more popular as an alternative to Integrated Pest Management. The purpose of this study was to evaluate the effect of leaf and fruit aqueous extracts, from the species Clibadium sylvestre, and leaves and root of Derris amazonica species at concentrations of 0, 1, 2, 4 and 8%, in the control of the aphid Myzus persicae (Hemiptera: Aphididae). Four experiments were conducted; two tests with choice preference and two tests with no choice preference, giving nine treatments and five repetitions. Phytochemical screening was carried out from the leaves and fruit of the species C. sylvestre and leaves and root of D. amazonica. Evaluations of mortality, number of nymphs, and deterrence index of the insects were conducted 24, 48 and 72 h after the application of the extracts. The aqueous extracts tested from C. sylvestre fruit concentrations had the highest mortality compared to the control; in the analysis of the number of the nymphs, the aqueous extract from fruit at 8% concentration had a higher efficiency than the other treatments. The D. amazonica leaves extract at 1% concentration showed the highest mortality and the lowest number of nymphs. The D. amazonica root extracts increased mortality at all concentrations tested, and the 8% concentration had the fewest nymphs. All treatments showed a deterrent effect. The 72 h period showed the greatest effect of the extracts, on two species of insects.


Author(s):  
O. R. Umeh ◽  
E. I. Chukwura ◽  
E. L. Okoye ◽  
E. M. Ibo ◽  
P. I. Egwuatu ◽  
...  

Medicinal plants are used by almost 80% of the world’s population for their basic health care because of their low cost and ease in availability. In the last few decades, many bacteria have continued to show increasing resistance against current antibiotics. Aim: In this study, phytochemical screening and antibacterial effects of conventional antibiotics, garlic and ginger on test isolates from fish pond water samples were evaluated between May-November, 2019. Methods: Standard methods for phytochemical screening and antibacterial analysis were employed. Results: The results showed that amongst the antibiotics used for susceptibility test, Amoxicilin (30 µg) was mostly resisted by all the bacterial isolates except Staphylococcus aureus, Vibrio cholerae, Vibrio parahaemolyticus and Salmonella typhi while erythromycin (10 µg) was unable to inhibit Bacillus subtilis. Ciprofloxacin (10 µg) and pefloxacin (10 µg) inhibited the growth of all the isolates except Pseudomonas aeruginosa. The test isolates showed variable susceptibility to the garlic and ginger extracts (ethanol, methanol and hot water). The extracts inhibited the isolates in descending order; ginger ethanol > ginger methanol > garlic methanol > ginger hot water > garlic ethanol > garlic hot water. Vibrio parahaemolyticus, Vibrio cholerae and Staphylococcus aureus showed little resistant to the extracts while these extracts showed better activity on Klebsiella pneumoniae and Proteus mirabilis. Synergistic effect of garlic and ginger (500mg/ml) inhibited the growth of all the isolates with ethanol extracts having the highest zone diameter (29 mm) on Klebsiella pneumoniae and Proteus mirabilis while hot water extracts had the least zone of inhibition (18 mm) on Acinetobacter calcoaceticus and Vibrio parahaemolyticus. The minimum inhibitory and bactericidal concentration for ethanol, methanol and hot water extracts ranged from 31.25mg/ml to 62.5mg/ml and 62.5mg/ml to 125mg/ml respectively. Conclusion: The outcomes of susceptibility experiment depicted that ethanol and methanol extracts of garlic and ginger (each alone and in combination) showed more inhibitory effect than aqueous extracts and also the combination of ethanol, methanol and aqueous extracts resulted in inhibitory effect greater than each of the extracts when used singly. The use of ginger and garlic for control of fish pathogens appears to be justified.


2021 ◽  
pp. 28-29
Author(s):  
Umadevi. D ◽  
K.U.M.A Rafeeq

Cymbopogon citratus, widely known as lemongrass is used as folk medicine for several disease conditions. Preliminary phytochemical screening of the essential oil extract of C. citratus revealed the presence of various bioactive compounds like alkaloids, avonoids, saponins, tannins and phenolic compounds in the aqueous extracts. Essential oil was extracted from C. citratus leaves and its chemical constituents were identied by Gas Chromatography-Mass Spectrometry (GC-MS).


2017 ◽  
Vol 89 (3 suppl) ◽  
pp. 2141-2154 ◽  
Author(s):  
MARÍLIA TEDESCO ◽  
ANDRIELLE W. KUHN ◽  
VIVIANE DAL-SOUTO FRESCURA ◽  
ALINE A. BOLIGON ◽  
MARGARETH L. ATHAYDE ◽  
...  

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Nelson Mpumi ◽  
Kelvin M. Mtei ◽  
Revocatus L. Machunda ◽  
Patrick A. Ndakidemi

The effectiveness of 1, 5, and 10% w/v of aqueous extracts of Croton dichogamus, Tephrosia vogelii, and Syzygium aromaticum and their mixture (2.5 and 5%) was evaluated against Myzus persicae on Brassica oleracea under field conditions. The synthetic chemical insecticide, chlorpyrifos, was used as positive control, and water and water plus soap were used as negative controls. Aqueous extracts significantly ( P ≤ 0.05 ) lowered M. persicae population compared with negative controls. The 5% of the aqueous extract from mixed plants and 10% of each plant used in this study significantly ( P ≤ 0.01 ) reduced aphid, M. persicae population comparable to chlorpyrifos in 2019 and 2020. However, the 1 and 5% concentrations of aqueous extracts of C. dichogamus, T. vogelii, and S. aromaticum and 2.5% of aqueous extracts from the mixed plants significantly lowered the population of M. persicae compared with negative controls in 2019 and 2020 wet seasons. The weekly observations revealed that, at weeks 1, 2, 3, 4, and 5 after application of treatments, the population of M. persicae differed significantly ( P ≤ 0.05 ). Also, the aqueous extracts from the mixed plants at 5% concentrations significantly ( P ≤ 0.01 ) reduced M. persicae population as compared with other treatments. It was as effective as synthetic chemical pesticide (chlorpyrifos). Also, 1 and 5% of C. dichogamus, T. vogelii, and S. aromaticum and the 2.5% of aqueous extracts from the mixed plants significantly reduced the population of M. persicae for all 6 weeks of study compared with negative controls. Therefore, these pesticidal plants can be recommended to smallholder farmers for the control of M. persicae in B. oleracea crops.


Author(s):  
Mikala Grâce Raycélia Merveille ◽  
Nkounkou Loumpangou Célestine ◽  
Kadja Amani Brice ◽  
Mamyrbekova-Békro Janat Akhanovna ◽  
Békro Yves-Alain ◽  
...  

Background: Despite advances in hygiene and preventive medicine, parasitic diseases, particularly digestive parasitosis, remain a public health problem in tropical countries. Elaborate the ethnobotanical survey carried out in Brazzaville - Congo, 21 plants (divided into 20 families, 20 genera) were identified, among them 9 were selected for this study. These were: Ageratum conyzoides (L.) L., Rauvolfia mannii Stapf, Aloe buettneri A. Berger, Garcinia kola Heckel, Piper guineense Schumach & Thonn., Aframomum alboviolaceum (Ridl.) K. Schum., Plagiostyles africana (Müll .Arg.) Prain, Morinda lucida Benth, Cogniauxia podoleana Baill. Materials and Methods: The in vitro evaluation of the anthelmintic activity of the aqueous extracts of these 9 plants was determined at concentrations of 10, 25 and 50 mg/mL against Lumbricus terrestris used as animal support in comparison to the Mebendazole (20 mg/mL). The phytochemical screening realized by TLC to detect phytochemical families in plant, also, the antioxidant potential with DPPH radical was determined by UV-Visible spectrophometry in comparison to the Vitamin C. Results: Thus, P. guineense, A. conyzoides and A. alboviolaceum are those which presented significant vermicidal effect, after Mebendazole. Phytochemical screening into TLC revealed the co-presence of alkaloids, coumarins, flavonoids, tannins, sterols and terpenes.The antioxidant potential of the aqueous extracts of these different plants in comparison to vitamin C, has shown that the species A. Buettineri and A. alboviolaceum presented the best potential compared to 7 other species studied, with the  concentration  needed to  reduce 50% of the DPPH radical (CR50)  respectively 0.096 mg/mL for A. Buettineri, 0.098 mg/mL for A. alboviolaceum Berger and 0.015 mg/mL for vitamin C. Conclusion: There is modern drugs against intestinal worms, it is necessary to identify the plants with anthelmintic activity, to determine phytochemical compounds that would be responsible for their use by traditional healers to treat the intestinal parasitosis.


2021 ◽  
Vol 4 (1) ◽  
pp. 11-15
Author(s):  
Kokou Atchou ◽  
Koku Amegbo Dakey ◽  
Farida Etroubeka-Tsono ◽  
Amégninou Agban ◽  
Simplice Damintoti Karou ◽  
...  

Staphylococcus aureus is a ubiquitous bacteria that causes a serious health problem because of its multi-resistance to antibiotics. The aimed of this study was to evaluate the antibacterial activity of P. kotschyi root against S. aureus ATCC 29213 and S. aureus, a clinical strain. The phytochemical compound was sought in the extracts by standard staining tests and extractions were carried out by fractionation using solvents depletion method with increasing polarity. The method of dilution in liquid medium was used for the antibacterial tests. The results showed that the aqueous extract of the bark was effective against both S. aureus with MICs of 0.39 mg.mL-1. The MICs of ethanolic extract were 0.39 and 0.78 mg.mL-1, respectively on S. aureus ATCC 29213 and S. aureus. The steles of P. kotschyi roots were less effective. The ethanolic and aqueous extracts at concentrations = 2MICs, have been respectively bacteriostatic and bactericidal effects on tested germs. The kinetics of inhibition showed that the aqueous extract of the bark at 0.78 mg.mL-1, completely destroyed the two germs respectively in 4 h and 5 h. The action of ethanolic extract at 0.78 and 1.56 mg.mL-1 occurs late against both S. aureus at 6 h and 7 h. Phytochemical screening revealed the presence of alkaloids, tannins, flavonoids, anthracenes, glycosides, saponosides and sterols, some of that may be responsible for the observed antimicrobial activity. This study proved the antibacterial activity of P. kotschyi roots that can be exploited as an antibiotic in the treatment of S. aureus infections.


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