Determination of antibacterial properties of silver nanoparticles with aqueous extracts of Brassica oleracea L. var. acephala D.C. in cotton textiles

Author(s):  
Alper ÇİFTCİ ◽  
Alper ÇİFTCİ ◽  
Engin Burgaz ◽  
Merve Gizem Sezener ◽  
Çigdem Dikbas ◽  
...  
METANA ◽  
2018 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Aldila Sagitaning Putri ◽  
Endang i Bekti Kristiani ◽  
Sri Haryati

Kubis Merah (Brassica oleracea L.) merupakan salah satu hasil pertanian yang mengandung kandungan antosianin yang berpotensi sebagai antioksidan. Tujuan yang hendak dicapai dalam penelitian ini adalah untuk menggali potensi kubis merah sebagai antioksidan alami pada kerupuk kubis merah. Tahapan dari penelitian ini adalah pembuatan kerupuk kubis merah dengan berbagai formulasi tepung tapioka dan kubis merah, uji organoleptik serta penentuan aktivitas antioksidan dan kadar antosianin. Hasil penelitian menunjukkan bahwa aktivitas antioksidan dari kubis merah dan kerupuk kubis merah sebesar. Dari uji organoleptik yang paling disukai panelis adalah perlakuan T2K1 yaitu 150 gram tapioka dan 50 gram kubis mera yang memiliki kadar air sebesar 15,49%, kadar abu 1,18%, kadar protein 1,48%, kadar lemak 16,55%, antosianin 1,31% dan aktivitas antioksidan sebesar 23,93%. Dengan demikian dapat disimpulkan bahwa kerupuk kubis merah memiliki potensi sebagai sumber antioksidan. Antioxidant content  in Red Cabbage  (Brassica oleracea L.) and Application In The Making of Crackers Red Cabbage (Brassica oleracea L.) is one of the agricultural products containing anthocyanin content that has potential as antioxidant. The purpose of  this study was to explore the potential of red cabbage as a natural antioxidant in red cabbage crackers. The stages of this research are the manufacture of red cabbage crackers with various formulations of tapioca flour and red cabbage, organoleptic test and the determination of antioxidant activity and anthocyanin levels. The results showed that antioxidant activity of red cabbage and red cabbage cracker amounted to. The most preferred organoleptic test of panelist is T2K1 treatment which is 150 g tapioca and 50 g of red cabbage which has water content 15,49%, ash content 1,18%, protein content 1,48%, fat content 16,55%, antocyanin 1.31% and antioxidant activity of 23.93%. Thus it can be concluded that red cabbage crackers have the potential as a source of antioxidants.


2020 ◽  
Vol 12 (24) ◽  
pp. 10523
Author(s):  
Alaa H. Alkhathlan ◽  
Hessah A. AL-Abdulkarim ◽  
Mujeeb Khan ◽  
Merajuddin Khan ◽  
Abdullah AlDobiy ◽  
...  

Applications of chemical synthetic methods for the preparation of metal nanoparticles involve toxic reagents, which are hazardous to both humans and the environment. On the other hand, ecofriendly plant-based techniques offer rapid, non-toxic, and suitable alternatives to the traditional methods. Herein, we report an eco-friendly method for the preparation of silver nanoparticles (Ag NPs) using two different aqueous extracts of Zingiber officinale (ginger) and Nigella sativa L. seeds (black cumin). Successful preparation of Ag NPs was confirmed by X-ray diffraction, ultraviolet–visible (UV-Vis) spectroscopy, and energy dispersive spectroscopy (EDX). Transmission electron microscopy (TEM) analysis revealed that Nigella sativa L. seed extract (NSE) produced a smaller size of NPs (~8 nm), whereas the ginger extract (GE) led to the formation of slightly larger Ag NPs (~12 nm). In addition, to study the effect of concentration of the extract on the quality of resulting NPs, two different samples were prepared from each extract by increasing the concentrations of the extracts while using a fixed amount of precursor (AgNO3). In both cases, a high concentration of extract delivered less agglomerated and smaller-sized Ag NPs. Furthermore, the antibacterial properties of as-prepared Ag NPs were tested against different bacterial strains. Notably, despite the slightly better quality of Ag NPs obtained from NSE (NSE-Ag), NPs prepared by using GE (GE-Ag) demonstrated superior antibacterial properties. In case of the plant-extract-based synthesis of nanoparticles, it is widely reported that during the preparation, the residual phytomolecules remain on the surface of resulting NPs as stabilizing agents. Therefore, in this case, the high antibacterial properties of GE-Ag can be attributed to the contributing or synergetic effect of residual phytomolecules of GE extract on the surface of Ag NPs, since the aqueous extract of GE has been known to possess higher intrinsic bactericidal properties when compared to the aqueous NSE extract.


2019 ◽  
Vol 9 (3) ◽  
pp. 393-397
Author(s):  
R. Sharma ◽  
J. Singh ◽  
A.K. Bhatia

Introduction: An alternative source of synythesis of nanoparticles is plant extract rather than chemical methods. This is because of presence of secondary metabolites or reducing agents in plant extract which are responsible for nanoparticles synthesis. In bioaccumulation, this synthesis depends upon the availability of particular enzymes or protein in plant extract. Materials & Methods: Considering the therapeutic potentials of nanoparticles, this work has been designed to find out antibacterial activity of silver nanoparticles. Objectives of this work are - preparation of silver nanoparticles chemically and biologically, characterisation of nanoparticles and evaluation of their antibacterial activities against E. coli. Comparision of antibacterial properties were made among NaBH4- AgNPs, Azadirachta indica (Neem) extract AgNPs and Brassica oleracea (Cauliflower) extract AgNPs. UV- absorption spectra of chemically and biologically synthesized AgNPs at different time intervals were measured using UV-Visible spectrophotometer. Particle size of AgNPs was measured by dynamic laser scattering technique (DLS) using Malvern Aimil Zetasizer. Results: The obtained silver nanoparticles were of sizes between 10 nm and 100 nm. Conclusion: It was clear from antibacterial activities that biologically synthesized AgNPs were more effective against E. coli than chemically synthesized AgNPs.


2012 ◽  
Vol 60 (21) ◽  
pp. 5380-5387 ◽  
Author(s):  
Carla Sousa ◽  
Fátima Fernandes ◽  
Patrícia Valentão ◽  
António Sebastião Rodrigues ◽  
Marta Coelho ◽  
...  

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