Green tea extract modulates lithium-induced thyroid follicular cell damage in rats

Iron is a crucial trace element and essential for many cellular processes; however, excessive iron accumulation can induce oxidative stress and cell damage. Neurodegenerative disorders, such as Alzheimer’s disease and Parkinson’s disease, have been associated with altered iron homoeostasis causing altered iron distribution and accumulation in brain tissue. This study aims to investigate the protective effect of 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) in combination with green tea extract (GTE) on iron-induced oxidative stress in neuroblastoma (SH-SY5Y) cells. Cells were cultured in medium with or without ferric chloride loading. Their viability and mitochondrial activity were assessed using MTT and JC-1 staining methods. Levels of the cellular labile iron pool (LIP), reactive oxygen species (ROS), and lipid-peroxidation products were determined using calcein acetoxymethyl ester, 2′,7′-dichlorohydrofluorescein diacetate, and TBARS-based assays, respectively. The viability of iron-loaded cells was found to be significantly increased after treatment with CM1 (10 µM) for 24 h. CM1 co-treatment with GTE resulted in a greater protective effect than their monotherapy. Combination of CM1 and GTE also reduced mitochondrial disruption and LIP content and ROS and TBARS production. In conclusion, the combination of CM1 and GTE exhibits protection against iron-induced oxidative stress in neuroblastoma cells.

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Green Tea Extract May Prevent PIN Progression

Family Practice News ◽

10.1016/s0300-7073(05)71574-7 ◽

2005 ◽

Vol 35 (16) ◽

pp. 48

Author(s):

ROBERT FINN

Keyword(s):

Green Tea ◽

Green Tea Extract ◽

Tea Extract

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Green Tea Extract Exposure Pattern Differenzially Affects Acetaminophen-Induced Hepatotoxicity

Planta Medica ◽

10.1055/s-0031-1273663 ◽

2011 ◽

Vol 77 (05) ◽

Author(s):

A Ali ◽

X Yang ◽

Q Shi ◽

J Greenhaw ◽

WF Salminen

Keyword(s):

Green Tea ◽

Green Tea Extract ◽

Exposure Pattern ◽

Tea Extract

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Immunological and redox effects of diets enriched in glutamine and antioxidants, a green tea extract diet and a combination of these diets on several organs during endotoxemia

Aktuelle Ernährungsmedizin ◽

10.1055/s-2006-946772 ◽

2006 ◽

Vol 31 (03) ◽

Author(s):

N Unger-Manhart ◽

M Esparrech ◽

B Wessner ◽

E Roth

Keyword(s):

Green Tea ◽

Green Tea Extract ◽

Tea Extract

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Antioxidative Activity of Laver Snack Prepared with Addition of Green Tea Extract

The Korean Tea Society ◽

10.29225/jkts.2017.23.4.35 ◽

2017 ◽

Vol 23 (4) ◽

pp. 35-41

Author(s):

Jeong Hee Park ◽

Hang Yeon Jeong ◽

Jeong Yong Cho ◽

Jae Hak Moon

Keyword(s):

Green Tea ◽

Antioxidative Activity ◽

Green Tea Extract ◽

Tea Extract

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N-hexane Extract and Fraction of Green Tea as Antiproliferation of MCM-B2 Breast Cancer Cells In Vitro

Current Biochemistry ◽

10.29244/cb.6.2.3 ◽

2020 ◽

Vol 6 (2) ◽

Author(s):

Lisni Noraida Waruwu ◽

Maria Bintang ◽

Bambang Pontjo Priosoeryanto

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Green Tea ◽

Cancer Cell ◽

Breast Cancer Cells ◽

Green Tea Extract ◽

Hexane Fraction ◽

Phytochemical Screening ◽

Tea Extract

Green tea (Camellia sinensis) is one of traditional plants that have the potential as an anticancer. The sample used in this research commercial green tea extract. The purpose of this study was to test the antiproliferation activity of green tea extract on breast cancer cell MCM-B2 in vitro. Green tea extract fractionated using three solvents, ie water, ethanol 70%, and n-hexane. Extract and fraction of green tea water have value Lethality Concentration 50 (LC50) more than 1000 ppm. The fraction of ethanol 70% and n-hexane had an LC50 value of 883.48 ppm and 600.56 ppm, respectively. The results of the phytochemical screening of green tea extract are flavonoids, tannins, and saponins, while the phytochemical screening results of n-hexane fraction are flavonoids and tannins. Antiproliferation activity was tested on breast cancer cells MCM-B2 and normal cells Vero by trypan blue staining method. The highest MCM-B2 cell inhibitory activity was achieved at a concentration of 13000 ppm green tea extract and 1000 ppm of n-hexane fraction, 59% and 59%, respectively. The extract and n-hexane fraction of green tea are not toxic to normal Vero cells characterized by not inhibiting normal cell proliferation. Keywords: antiproliferative, cancer cell MCM-B2, commercial green tea, cytotoxicity

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Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production

Mansoura Veterinary Medical Journal ◽

10.35943/mvmj.2020.21.105 ◽

2020 ◽

Vol 21 (1) ◽

pp. 31-35

Author(s):

Basma El-Desoky ◽

Shaimaa El-Sayed ◽

El-Said El-Said

Keyword(s):

Gene Expression ◽

Single Dose ◽

Green Tea ◽

Serum Testosterone ◽

Green Tea Extract ◽

Male Rats ◽

Controlled Study ◽

Control Group ◽

Testicular Damage ◽

Tea Extract

Objective: Investigating the effect of green tea extract (GTE) on the testicular damage induced by cadmium chloride CdCl2 in male rats. Design: Randomized controlled study. Animals: 40 male Wistar rats. Procedures: Rats were randomly divided into four groups: A) control group (each rat daily received pellet diet); B) GTE group each rat daily received pellet diet as well as 3 ml of 1.5 % w/v GTE, C) CdCl2 group each rat was I/P injected a single dose of 1 mg/kg CdCl2, then daily received pellet diet, and D) CdCl2+GTE group each rat was I/P injected a single dose of 1 mg/kg CdCl2 then daily received pellet diet as well as 3 ml of 1.5 % w/v GTE. After 30 days, blood samples were collected for hormonal assays (testosterone, FSH, and LH). In addition, both testes were collected; one of them was used for quantification of 17-beta hydroxysteroid dehydrogenase III (17β-HSDIII) gene expression using a real-time PCR. The other testis was used for determination of catalase and reduced glutathione; GSH, Nitric oxide (NO) and malondialdehyde (MDA) levels. Results: CdCl2 decreased serum testosterone levels and its synthesis pathway (17β-HSDIII testicular gene expression). While antioxidants catalase and GSH were reduced, oxidants MDA were enriched in the testes of CdCl2-poisoned rats. This CdCl2-promoted testicular dysfunction was corrected via the administration of GTE to male rats. Conclusion and clinical relevance: GTE could be used as a remedy for protecting against CdCl2-induced testicular damage in male rats.

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