Morphological examination of the accessory sex glands of the Barki bucks (Capra hircus)

Regression of the accessory sex glands was induced in male Syrian hamsters by chronic exposure to diethylstilboestrol (DES), an agonist of 17β-oestradiol. Experimental groups (n = 4–5) were killed at increasing time intervals up to 6 months after initiation of treatment. Organ atrophy was evaluated by morphological examination. Apoptosis in the seminal vesicles and coagulating glands was visualized by in situ analysis of DNA fragmentation. Cell proliferation was monitored by immunostaining nuclei of S-phase cells after pulse labelling with BrdU. The volume of the seminal vesicles decreased drastically after 2 weeks of DES administration due to a marked reduction of secretions in the lumen of the glands. Cell proliferation in the seminal vesicles was stimulated by chronic administration of DES. Mitotic activity mostly increased during the first month of treatment, leading to epithelial hyperplasia associated with progressive hyperplasia of the fibromuscular stroma. Evidence of epithelial dysplasia and metaplasia, often associated with an infiltration of polymorphonuclear leukocytes, was observed in animals exposed to DES for 4 months or more. Regression of the seminal vesicles was also associated with apoptosis in the gland epithelium. Apoptosis appeared 3 days after starting DES administration and culminated at 1 month. Thereafter the number of apoptotic cells decreased progressively, but apoptosis remained present until the end of treatment. In contrast, coagulating glands were less sensitive to DES. No major morphological changes were observed in these glands except for a moderate reduction of protein secretion. The levels of the apoptotic and proliferating cells indices were very low in the coagulating glands during DES treatment. In conclusion, these data point to different sensitivities of the accessory sex glands to DES exposure because DES induces extensive alterations of the normal morphology of the seminal vesicles, but shows only a moderate impact on the coagulating glands. Extra keyword: oestrogen.

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Morphological Examination of a Herpes-type Virus Indigenous for Tree Shrews

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100067790 ◽

1970 ◽

Vol 28 ◽

pp. 160-161

Author(s):

J. P. Brunschwig ◽

R. M. McCombs ◽

R. Mirkovic ◽

M. Benyesh-Melnick

Keyword(s):

Electron Microscopy ◽

Soft Tissue ◽

Chick Embryo ◽

Soft Tissue Sarcoma ◽

Cell Cultures ◽

Tree Shrew ◽

Type Virus ◽

Morphological Examination ◽

Tree Shrews ◽

Embryo Cells

A new virus, established as a member of the herpesvirus group by electron microscopy, was isolated from spontaneously degenerating cell cultures derived from the kidneys and lungs of two normal tree shrews. The virus was found to replicate best in cells derived from the homologous species. The cells used were a tree shrew cell line, T-23, which was derived from a spontaneous soft tissue sarcoma. The virus did not multiply or did so poorly for a limited number of passages in human, monkey, rodent, rabbit or chick embryo cells. In the T-23 cells, the virus behaved as members of the subgroup B of herpesvirus, in that the virus remained primarily cell associated.

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FR-IR Molecular Microanalysis System

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100134958 ◽

1990 ◽

Vol 48 (2) ◽

pp. 270-271

Author(s):

John A. Reffner ◽

William T. Wihlborg

Keyword(s):

Fourier Transform ◽

Fourier Transform Infrared ◽

Integrated System ◽

Morphological Examination ◽

Fully Integrated ◽

Ir Microscopy ◽

Normal Functions ◽

Infrared Microspectroscopy ◽

Infrared Spectral ◽

Ft Ir

The IRμs™ is the first fully integrated system for Fourier transform infrared (FT-IR) microscopy. FT-IR microscopy combines light microscopy for morphological examination with infrared spectroscopy for chemical identification of microscopic samples or domains. Because the IRμs system is a new tool for molecular microanalysis, its optical, mechanical and system design are described to illustrate the state of development of molecular microanalysis. Applications of infrared microspectroscopy are reviewed by Messerschmidt and Harthcock.Infrared spectral analysis of microscopic samples is not a new idea, it dates back to 1949, with the first commercial instrument being offered by Perkin-Elmer Co. Inc. in 1953. These early efforts showed promise but failed the test of practically. It was not until the advances in computer science were applied did infrared microspectroscopy emerge as a useful technique. Microscopes designed as accessories for Fourier transform infrared spectrometers have been commercially available since 1983. These accessory microscopes provide the best means for analytical spectroscopists to analyze microscopic samples, while not interfering with the FT-IR spectrometer’s normal functions.

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COMPARATIVE EVALUATION OF MULTIPARAMETRIC ENDORECTAL ULTRASOUND AND ENHANCED IMAGING COLONOSCOPY IN THE DIAGNOSIS OF EARLY COLORECTAL CANCER

Koloproktologia ◽

10.33878/2073-7556-2020-19-3-49-64 ◽

2020 ◽

Vol 19 (3) ◽

pp. 49-64

Author(s):

E. M. Bogdanova ◽

Yu. L. Trubacheva ◽

O. M. Yugai ◽

S. V. Chernyshov ◽

E. G. Rybakov ◽

...

Keyword(s):

Colorectal Cancer ◽

Statistical Significance ◽

Muscle Layer ◽

Endorectal Ultrasound ◽

Diagnostic Methods ◽

Early Colorectal Cancer ◽

Morphological Examination ◽

Optical Magnification ◽

The Difference ◽

Enhanced Imaging

AIM: to compare multiparametric endorectal ultrasound (ERUS) and enhanced imaging colonoscopy in the diagnosis of early colorectal cancer.PATIENTS AND METHODS: the study included 78 patients with epithelial rectal tumor. All the patients underwent multiparametric ERUS and colonoscopy with examination by narrow beam imaging (NBI) at optical magnification. All the patients were operated.RESULTS: a morphological examination removed specimens revealed adenomas in 48 cases, in 19 specimens – adenocarcinomas in situ and T1, and in 11 specimens – adenocarcinomas with invasion of the muscle layer or deeper. When calculating the accuracy indicators of diagnostic methods for groups of patients with adenoma, Tis-T1 adenocarcinoma, and T2-T3 adenocarcinoma, the difference in the sensitivity and specificity of the methods in none of the presented groups did not reach the level of statistical significance (p>0.05).ROC analysis showed that ultrasound has a prognostic value comparable to colonoscopy. The area difference was 0.013 (p=0.85).CONCLUSION: endoscopy and ultrasound have similar value in the diagnosis of malignant transformation of rectal adenomas.

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A technique for superfusion of isolated granulosa cells. Dynamics of cAMP production and steroidogenesis in response to gonadotropins

Acta Endocrinologica ◽

10.1530/acta.0.1170497 ◽

1988 ◽

Vol 117 (4) ◽

pp. 497-506 ◽

Cited By ~ 4

Author(s):

Carl Johanson ◽

Viktor Johanson

Keyword(s):

Granulosa Cells ◽

Culture Medium ◽

Cell Damage ◽

Adenosine Monophosphate ◽

Camp Production ◽

Morphological Examination ◽

Ovarian Cells ◽

Temporal Relationships ◽

Polycarbonate Membranes ◽

Estradiol 17Β

Abstract. A superfusion model for isolated ovarian cells was developed and characterized in detail. Granulosa cells isolated from pre-ovulatory rat ovarian follicles were placed in superfusion (perifusion) chambers with a volume of 125 μl. Culture medium was pumped through the chambers, collected in 20-min fractions of 600 μl and analysed for cAMP and steroids. Viability was confirmed by morphological examination. The use of polycarbonate membranes to retain the cells in the chambers was abandoned since the membranes caused severe cell damage. The temporal relationships between gonadotropic stimuli and the release of cyclic 3':5'-adenosine monophosphate (cAMP) and steroids was investigated. Within 10 min FSH elicited transient increase in the release of cAMP and progesterone but had no effect on testosterone or estradiol-17β release. Amplitude and duration of the response in cAMP and progesterone release were correlated to concentration and length of the FSH pulse when these parameters were varied within the ranges 1–100 μg/l and 30–270 min, respectively. Compared with the cAMP response, the progesterone response peaked up to 30 min later and lasted 1 to 2 h longer but could not be extended to more than approximately 6 h, not even with longer FSH pulses. These results could indicate a development of desensitization.

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Effect of Nutritional Stress on Allometric Parameters of Non Descript Indian Buck(Capra Hircus) in a Controlled Thermoneutral Condition

Global Journal For Research Analysis ◽

10.15373/22778160/july2014/1 ◽

2012 ◽

Vol 3 (7) ◽

pp. 1-2

Author(s):

Hari abdul Samad ◽

◽

Shyma K latheef ◽

Anuraj K. S Anuraj K. S ◽

V. P. Maurya V. P. Maurya

Keyword(s):

Nutritional Stress ◽

Capra Hircus

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СРАВНИТЕЛЬНЫЙ АНАЛИЗ ГЕННЫХ КАРТ Bos taurus L. И Capra hircus L.

Sel skokhozyaistvennaya Biologiya ◽

10.15389/agrobiology.2013.2.63rus ◽

2013 ◽

pp. 63-70

Author(s):

Л.К. ЭРНСТ ◽

◽

П.М. КЛЕНОВИЦКИЙ ◽

В.А. БАГИРОВ ◽

Б.С. ИОЛЧИЕВ ◽

...

Keyword(s):

Bos Taurus ◽

Capra Hircus

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Morphological features of endo- and myometrium in patients with uterine leiomyoma and infertility

HEALTH OF WOMAN ◽

10.15574/hw.2020.151-152.33 ◽

2020 ◽

pp. 33-37

Author(s):

M.A. Flaksenberg ◽

◽

Keyword(s):

Uterine Leiomyoma ◽

Negative Impact ◽

Reproductive Function ◽

Cell Types ◽

Underlying Disease ◽

Reproductive Organs ◽

Pathological Process ◽

Simple Type ◽

Morphological Examination ◽

Blastocyst Implantation

The objective: determination of morphofunctional features of leiomatous nodes and endometrium in women with uterine leiomyoma and infertility to restore reproductive function and prevent recurrence of the underlying disease. Materials and methods. In order to restore reproductive function and prevent recurrence of the underlying disease, morphofunctional features of leiomatous nodes and endometrium in women with uterine leiomyoma and infertility were determined. Thirty samples of leiomyomatous nodes and endometrium were examined, among which 15 were obtained from women with multiple uterine leiomyoma and infertility and 15 samples from women with uterine leiomyoma with isolated uterine leiomyoma. During the study, a general-histological method was used for staining with hematoxylin-eosin and picrofuxin by van Gizon, as well as immunohistochemical methods. Histological examination of the endometrium was performed according to conventional protocol, taking into account the day of the menstrual cycle and R.W. Noyes criteria. Results. In the morphological examination of leiomyomatous nodes in the vast majority of cases the presence of uterine leiomyomas of simple and cell types or their combination was established. In women with multiple uterine leiomyoma, simple-type leiomyoma (53.3%) was predominant, and in patients with isolated leiomyoma the signs of cellular uterine leiomyoma (66.7%) were more frequently found. In 80.0% of women with uterine leiomyoma revealed pathology of the endometrium, such as glandular and glandular-fibrous polyps, simple and complex atypical endometrial hyperplasia, which confirms the theory about the only pathogenetic mechanisms of the emergence of hyperplastic processes of female organs. In 66.7% of women with multiple leiomyomas, signs of chronic endometritis have been found, which exacerbates the pathological process and can have a negative impact on the reproductive function, such as secretory endometrial transformation and impaired blastocyst implantation, and explains a much higher percentage of infertility in the group. Conclusion. In women with impaired reproductive function, patients with uterine leiomyoma, it is necessary to conduct a study of the receptivity of the reproductive organs, namely - the endometrium and leiomatous nodes. This will make it possible to use one or another method of treatment in order to restore reproductive function and prevent recurrence of the underlying disease. Keywords: infertility, uterine leiomyoma, endometrium, receptive apparatus.

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Size and density of a feral goat Capra hircus population in a protected area in the Pyrenees

Wildlife Biology in Practice ◽

10.2461/wbp.2013.ibeun.1 ◽

2013 ◽

Vol 9 (3) ◽

Author(s):

Juan Herrero ◽

Olatz Fernández ◽

Carlos Prada ◽

Alicia García-Serrano

Keyword(s):

Protected Area ◽

Capra Hircus ◽

Feral Goat

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