scholarly journals Effects of Type and Level of Forage Supplementation on Voluntary Intake, Digestion, Rumen Microbial Protein Synthesis and Growth in Sheep Fed a Basal Diet of Rice Straw and Cassava

1998 ◽  
Vol 11 (6) ◽  
pp. 692-696 ◽  
Author(s):  
S. Premaratne ◽  
J. van Bruchem ◽  
X. B. Chen ◽  
H. G. D. Perera ◽  
S. J. Oosting
Keyword(s):  
Protein Synthesis ◽  
Rice Straw ◽  
Basal Diet ◽  
Microbial Protein ◽  
Microbial Protein Synthesis ◽  
Voluntary Intake

The influence of supplementing rapidly degraded fibre with different forms of nitrogen on microbial protein synthesis in sheep

1998 ◽  
Vol 1998 ◽  
pp. 92-92
Author(s):  
S. Chikunya ◽  
E. L. Miller
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Microbial Activity ◽  
Basal Diet ◽  
Microbial Protein ◽  
Purine Derivative ◽  
Protein Requirements ◽  
Microbial Protein Synthesis ◽  
Current Systems

There is controversy in the literature concerning the effect of amino acid (AA) and peptide nitrogen on microbial activity in ruminants fed diets high in structural carbohydrates. Microbial protein is the most variable and uncertain element of current systems of evaluating protein requirements for ruminants (ARC, 1984). Some of the variability is attributed to the multiplicity of techniques for estimating microbial yield, some of which are both imprecise and cumbersome. This study investigates the effects of different forms of nitrogen on microbial activity in sheep fed a rapidly degraded fibrous basal diet and attempts to validate the recently developed purine derivative (PD) technique by comparing it to other procedures used to estimate microbial protein synthesis under the same conditions.

Effect of banana flower powder on rumen fermentation, synthesis of microbial protein and nutrient digestibility in swamp buffaloes

2019 ◽  
Vol 59 (9) ◽  
pp. 1674 ◽  
Author(s):  
Metha Wanapat ◽  
Thiwakorn Ampapon ◽  
Kampanat Phesatcha ◽  
Sungchhang Kang
Keyword(s):  
Fatty Acids ◽  
Protein Synthesis ◽  
Rice Straw ◽  
Methane Production ◽  
Volatile Fatty Acids ◽  
Nutrient Digestibility ◽  
Microbial Protein ◽  
Microbial Protein Synthesis ◽  
Ruminal Ph ◽  
Banana Flower

Replacement of chemical compounds by dietary sources as rumen enhancers have been of great interest and concern by researchers. Four, rumen-fistulated swamp buffalo bulls with average liveweight of 365 ± 15.0 kg were randomly assigned to treatments, to investigate the impact of banana flower powder (BAFLOP) as a rumen modifier on pH, rumen fermentation, nutrient digestibility, microbial protein synthesis and volatile fatty acids. All buffaloes were allotted according to a 4 × 4 Latin square design. Dietary supplementation treatments were as follows: 2 g concentrate/kg bodyweight (BW; T1), 15 g concentrate/kg BW (T2), 15 g concentrate/kg BW plus BAFLOP 300 g/head.day (T3) and 15 g concentrate/kg BW plus BAFLOP 600 g/head.day (T4). Untreated rice straw was fed ad libitum. The findings showed that total feed intake was increased in buffaloes fed a diet supplemented with concentrate at 2 g/kg BW, while rice straw intake was reduced. Nutrient digestibility was increased by BAFLOP supplementation at both levels (T3 and T4; P < 0.05). Ruminal pH dropped (5.9) in buffaloes fed with concentrate at 15 g/kg BW, while buffaloes with BAFLOP supplementation could maintain ruminal pH when fed with high-concentrate diet. Ruminal ammonia-nitrogen increased in the buffaloes fed concentrate at 15 g/kg BW, especially with BAFLOP supplementation. Feeding high-concentrate diet increased the concentrations of ruminal total volatile fatty acids and propionic acid (C3), while the concentration of acetic acid and the acetic acid:C3 ratio and methane production were subsequently reduced (P < 0.05). In addition, efficiency of microbial protein synthesis was increased by the BAFLOP feeding (P < 0.05). In the present study, using BAFLOP as a dietary rumen enhancer at 300–600 g/head.day resulted in an increased rumen pH, C3 concentration, nutrient digestibility and microbial protein synthesis, while mitigating ruminal methane production. Higher nutrient digestibility and lower ruminal methane production, more dietary energy and production efficiency are expected.

scholarly journals The efficiency of microbial protein synthesis in the rumen and the degradability of feed nitrogen between the mouth and abomasum in steers given different diets

1984 ◽  
Vol 51 (1) ◽  
pp. 77-83 ◽  
Author(s):  
A. B. McAllan ◽  
R. H. Smith
Keyword(s):  
Protein Synthesis ◽  
Fish Meal ◽  
Basal Diet ◽  
Metabolizable Energy ◽  
Diaminopimelic Acid ◽  
Barley Straw ◽  
Microbial Protein ◽  
Total N ◽  
Microbial Protein Synthesis ◽  
Mixed Bacteria

1. Protozoa-free steers with simple rumen and abomasal cannulas were given basal diets consisting of a concentrate mixture of flaked maize and tapioca with barley straw (BS) or alkali-treated barley straw (BSA). Other diets were supplemented with urea (BSU and BSAU respectively) or with fish meal replacing the tapioca (BSF and BSAF respectively). The diets were isoenergetic and calculated to provide sufficient metabolizable energy (ME) to support a growth rate of 0.5 kg/d. Rumen-degradable nitrogen (RDN): ME values (g/MJ) were estimated to be 0.50, 1.20 and 0.80 for the basal diet, urea- and fish-meal-supplemented diets respectively. RNA and α, ε-diaminopimelic acid (DAP) were used as microbial markers. 103Ruthenium and polyethylene glycol (PEG) were given as flow markers and flows (g/24 h) at the abomasum of organic matter (OM) and nitrogenous constituents were calculated.2. Samples of mixed bacteria separated from rumen digesta from animals receiving N-supplemented diets contained significantly more N than those from animals receiving basal diets (approximately 74 and 62 mg/g dry matter (DM) respectively) but there were no other significant differences in total-N contents between treatments. RNA-N: total-N values were similar for all diets (approximately 0.13). DAP-N: total-N values were significantly lower in bacteria from animals receiving alkali-treated (AT) rather than untreated (UT) straw (approximately 0.008 and 0.011 respectively).3. The proportion of OM intake digested in the rumen (ADOM) was significantly higher for animals receiving AT straw rather than UT straw (approximately 0.54 and 0.43 respectively). N supplementation had no effect on OM digestibility.4. When basal rather than N-supplemented diets or AT-straw- rather than UT-straw-containing diets were given, there were significantly lower flows of ammonia-N, non-ammonia-N (NAN) and microbial-N (based on RNA flow, MN(RNA)) at the abomasum. Mean daily MN(RNA) flows (g) were 21, 30, 31, 16, 27 and 28 for diets BS, BSU, BSF, BSA, BSAU and BSAF respectively. These correspond to estimated efficiencies of microbial protein synthesis, expressed as g MN(RNA) /kg truly-digested OM, at 14, 22, 22, 12, 18 and 19 respectively. Values were significantly lower for basal as compared with corresponding N-supplemented diets and for AT-straw diets as compared with corresponding UT-straw diets.5. Estimated mean proportions of total feed-N intake degraded in the rumen, based on MN(RNA) as microbial marker, of diets BS, BSU and BSF were 0.60, 0.74 and 0.47 respectively; corresponding values for diets BSA, BSAU and BSAF were 0.72, 0.73 and 0.36 respectively. Making certain assumptions, the mean proportions of fish-meal-N digested in the rumen were calculated to be 0.23 and 0.14 respectively for UT- and AT-straw diets. The values were not significantly different. Values for microbial flows based on DAP as marker were significantly lower, by about 25%, than those based on RNA.

scholarly journals Peningkatan Kualitas Jerami Padi dan Pengaruhnya Terhadap Kecernaan Nutrien dan Produk Fermentasi Rumen Kerbau dengan Feces Sebagai Sumber Inokulum

2013 ◽  
Vol 13 (2) ◽  
pp. 59-67 ◽  
Author(s):  
Syapura Syapura ◽  
Muhamad Bata ◽  
Wardhana Surya Pratama
Keyword(s):  
Protein Synthesis ◽  
Organic Matter ◽  
Rice Straw ◽  
Dry Matter ◽  
Nutrient Digestibility ◽  
Microbial Protein ◽  
Fermentation Products ◽  
Microbial Protein Synthesis ◽  
Organic Matter Digestibility

Improving of rice straw quality and its effect on ability nutrient digestibility and rumen metabolism products of buffalo in-vitro with feces as inoculum source ABSTRACT.  This study was aimed to determine the effect of feeding  ammoniated rice straw plus concentrate on buffalo nutrient digestibility and rumen fermentation products by in vitro. The Research was carried out by using  experimental method, designed according to completely  randomized design (CRD). The source of inoculum was obtain from different feces of three  buffalos kept in  Datar Village of Purwokerto region fed  rice straw, rice straw plus concentrate and rice straw ammoniated plus concentrate with dry matter ratio of 80 : 20. The treatments tested consisted of three treatments, namely R0 =  control feed using rice straw; R1 = the use of rice straw plus concentrate with a ratio of  (DM basis) 80:20; R2 = the use of ammoniated rice straw plus concentrate with a ratio of (DM basis) 80:20. The treatments were repeated 7 times, so there were 21 experimental units. The Variables measured included total VFA, Ratio A/P, N-NH3, Microbial Protein Synthesis (MPS),   Dry Matter and Organic Matter Digestibility. The result of this study showed that the treatment had an effect significant (P0.05) on the concentration of VFA, Ratio A/P,  N-NH3,  Microbial Protein Synthesis (MPS), and Dry Matter and Organic Matter Digestibility. The HSD test showed that the highest production of  VFA,Ratio A/P, N-NH3, Microbial Protein Synthesis (MPS), Dry Matter and Organic Matter Digestibility were achieved at R2 followed by R1 and R0 respectively. The conclusion is that the ammoniated rice straw supplemented with concentrate can be recommended to be fed to buffalo

Effects of the ratio of effective rumen degradable protein to fermentable metabolizable energy on voluntary intake and milk yields of dairy cows

2001 ◽  
Vol 2001 ◽  
pp. 184-184
Author(s):  
A.R.J. Cabrita ◽  
A.J.M. Fonseca ◽  
C. Sampaio ◽  
E. Gomes ◽  
R.J. Dewhurst
Keyword(s):  
Protein Synthesis ◽  
Dairy Cows ◽  
Metabolizable Energy ◽  
Microbial Protein ◽  
Maize Silage ◽  
Protein Sources ◽  
Microbial Protein Synthesis ◽  
Voluntary Intake ◽  
Production Response ◽  
Degradable Protein

Diets with low ratios of effective rumen degradable protein (ERDP) to fermentable metabolizable energy (FME) are often offered to dairy cows in Portugal, because they are based on maize silage and protein sources are very expensive. It seems likely that this will restrict microbial protein synthesis and voluntary intake and, consequently, lead to reduced milk yields. The objective of this study was to examine the production response of dairy cows offered diets differing in ERDP/FME ratio.

Rumen fermentation and microbial protein synthesis in cattle given intraruminal infusions of lactic acid with a grass silage based diet

1992 ◽  
Vol 119 (3) ◽  
pp. 411-418 ◽  
Author(s):  
S. Jaakkola ◽  
P. Huhtanen
Keyword(s):  
Protein Synthesis ◽  
Lactic Acid ◽  
Volatile Fatty Acids ◽  
Control Diet ◽  
Basal Diet ◽  
Rumen Fermentation ◽  
Microbial Protein ◽  
Acid Fermentation ◽  
Microbial Protein Synthesis ◽  
Grass Silage

SUMMARYFour Friesian bulls with ruminal and duodenal cannulae were used in a 4 × 4 Latin square experiment to study the effects of lactic acid (LA) on rumen fermentation and microbial protein synthesis. On a dry matter (DM) basis (g/kg), the basal diet comprised grass silage (700), barley (240) and rapeseed meal (60) and it was given at the rate of 7·1 kg DM/day. LA was infused continuously into the rumen at the rates of 0 (L0), 40 (L40), 80 (L80) or 120 (L120) g/kg basal diet DM.The molar proportion of propionate in the rumen volatile fatty acids (VFA) increased linearly (P < 0.001) and that of acetate, isovalerate, caproate (P < 0.01) and isobutyrate (P < 005) decreased linearly with an increasing rate of LA infusion. At the same time there was a linear decrease (P < 0.05) in the number of rumen protozoa. When the metabolic fate of infused LA was calculated on a molar basis, 0.21 of lactic acid was converted to acetate, 0·52 to propionate and 0.27 to butyrate.Infusion of LA into the rumen had no effect on the site or extent of the digestion of basal diet organic matter (OM) and neutral detergent fibre (NDF). LA diets tended to have a lower microbial N flow at the duodenum (71·4 v. 85·8 g N/day) and lower synthetic efficiency in the rumen (14·4 v. 20.4 g N/kg OM apparently fermented) when compared with the control diet. The ratio of duodenal non-ammonia N to N intake was highest with the control diet and lowest with L40, the effect of the LA rate being quadratic (P < 0·05). The results suggest that propionate was the main end-product of lactic acid fermentation in the rumen with the grass silage based diet. Lactic acid had no value as an energy source for microbial protein synthesis.

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