Lactate Level in Amniotic Fluid, a New Diagnostic Tool

We evaluated the influence of hypoxic hypoxia on lactate, creatinine and urea concentrations in the amniotic fluid (AF) of rabbits on 27-28th day of pregnancy. Rabbits were randomly sudivided into two groups: experimental (n=9) and control (n=6). Rabbits of experimental groups were placed in a hypoxic chamber containing 10±2% oxygen and 90±2% nitrogen for 1 h and then were euthanized, AF was extracted from the amniotic sacs via disposable syringe. Acute hypoxic hypoxia had no effect on the AF volume, increased (1.4-fold) lactate, (1.3-fold) creatinine and (1.1-fold) urea concentrations in AF. In contrast to animals of the control group, lactate concentration in the groups with hypoxic hypoxia correlated with the creatinine (r=0.71, p<0.0001, n=35) and urea concentrations in the AF (r=0.81, p<0.0001, n=35). These results suggest that acute hypoxic hypoxia in late pregnancy causes changes in the biochemical composition of AF; these changes are characterized by high lactate concentrations, and the fetus and uterus can be the source of increased lactate level in AF.

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To find the association between intrapartum amniotic fluid lactate level and labour outcome

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20170394 ◽

2017 ◽

Vol 6 (2) ◽

pp. 620

Author(s):

Aravind Chander ◽

Seetha Panicker ◽

Reshma Shri

Keyword(s):

Amniotic Fluid ◽

Lactate Level ◽

Mode Of Delivery ◽

Rank Correlation ◽

Chi Square ◽

Active Labour ◽

Active Labor ◽

Chi Square Test ◽

Lactate Levels ◽

Supplementary Test

Background: Inspite of good monitoring in labour, there are still adverse labour outcomes. There is a need for supplementary test for early prediction of prolonged labour and fetal hypoxia. This study was undertaken to find to find the association between intrapartum amniotic fluid lactate level and labour outcome.Methods: 103 antenatal patients low risk at term, who entered into active labour either spontaneously or induced between September 2012 to August 2013 were included. Amniotic fluid was collected after doing ARM and lactate level was measured. Chi square test was used to find correlation between lactate level and duration of labour, mode of delivery and Apgar score.Results: In our study, patients with duration of labour between 8-<20hours 88 % had lactate level <10mmol/l, thus intra partum amniotic fluid lactate levels had a good negative predictive value when it was compared with the duration of labour. When the mode of delivery was analysed using Chi square test there was no statistically significant correlation (p<0.825) with amniotic fluid lactate. Spearman Rank correlation was done to observe correlation between lactate levels and Apgar which showed a correlation of 0.20 with p<0.05.Conclusions: Amniotic fluid lactate levels had better correlation with duration of labour and Apgar score. The lower the amniotic fluid lactate level, the better is the Apgar at birth. Better correlation could have been observed if amniotic fluid lactate levels were repeated every 3-4 hours during active labor. However, this was not possible due to practical difficulty in collection of sample.

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Stem cells in the amniotic fluid: the new chance of regenerative medicine

Orvosi Hetilap ◽

10.1556/oh.2011.29082 ◽

2011 ◽

Vol 152 (15) ◽

pp. 581-587 ◽

Cited By ~ 1

Author(s):

József Gábor Joó

Keyword(s):

Stem Cells ◽

Regenerative Medicine ◽

Amniotic Fluid ◽

Diagnostic Tool ◽

Congenital Malformations ◽

Genetic Diseases ◽

Storage Diseases ◽

Amniotic Fluid Stem Cells ◽

Amniotic Fluid Stem Cell ◽

Near Future

Amniotic fluid has been used in prenatal diagnosis for more than decades. It yields a simple and reliable screening and diagnostic tool for a variety of congenital malformations and genetic diseases such as chromosomal aberrations, neural tube defects or storage diseases. Nowadays the widening knowledge provides evidence that amniotic fluid is not only a screening and diagnostic tool, but it may be also the source of the effective therapy of several congenital and adult disorders. A subset of cells, the so-called stem cells were found in the amniotic fluid as well as the placenta, and they proved to be capable of maintaining prolonged undifferentiated proliferation. Stem cells are able to differentiate into multiple tissue types, originating from the three germ layers. In the near future stem cells isolated from amniotic fluid or placenta and stored by cryopreservation may play a significant role in regenerative medicine. Congenital malformations as well as certain diseases in adults might be treated by tissues coming from progenitor cells of amniotic fluid stem cell origin. This study gives a summary of the main characteristics of amniotic fluid stem cells and it also presents important examples of their possible clinical application. Orv. Hetil., 2011, 152, 581–587.

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The Elicited Language Analysis Procedure

Language Speech and Hearing Services in Schools ◽

10.1044/0161-1461.1301.37 ◽

1982 ◽

Vol 13 (1) ◽

pp. 37-41

Author(s):

Larry J. Mattes

Keyword(s):

Diagnostic Tool ◽

Descriptive Analysis ◽

Quality Measures ◽

Analysis Procedure ◽

Elicited Imitation ◽

Response Quality ◽

Semantic Relationships ◽

Language Analysis

Elicited imitation tasks are frequently used as a diagnostic tool in evaluating children with communication handicaps. This article presents a scoring procedure that can be used to obtain an in-depth descriptive analysis of responses produced on elicited imitation tasks. The Elicited Language Analysis Procedure makes it possible to systematically evaluate responses in terms of both their syntactic and semantic relationships to the stimulus sentences presented by the examiner. Response quality measures are also included in the analysis procedure.

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