scholarly journals Acute hypoxic hypoxia increases lactate concentration in amniotic fluid of rabbits on 27-28th day of pregnancy

2017 ◽  
Vol 63 (1) ◽  
pp. 81-84
Author(s):  
A.V. Popovtseva ◽  
E.V. Suzopov ◽  
Yu.V. Korenovsky
Keyword(s):  
Amniotic Fluid ◽  
Lactate Level ◽  
Biochemical Composition ◽  
Lactate Concentration ◽  
Late Pregnancy ◽  
Hypoxic Hypoxia ◽  
Control Group ◽  
Acute Hypoxic Hypoxia ◽  
And Control ◽  
High Lactate

We evaluated the influence of hypoxic hypoxia on lactate, creatinine and urea concentrations in the amniotic fluid (AF) of rabbits on 27-28th day of pregnancy. Rabbits were randomly sudivided into two groups: experimental (n=9) and control (n=6). Rabbits of experimental groups were placed in a hypoxic chamber containing 10±2% oxygen and 90±2% nitrogen for 1 h and then were euthanized, AF was extracted from the amniotic sacs via disposable syringe. Acute hypoxic hypoxia had no effect on the AF volume, increased (1.4-fold) lactate, (1.3-fold) creatinine and (1.1-fold) urea concentrations in AF. In contrast to animals of the control group, lactate concentration in the groups with hypoxic hypoxia correlated with the creatinine (r=0.71, p<0.0001, n=35) and urea concentrations in the AF (r=0.81, p<0.0001, n=35). These results suggest that acute hypoxic hypoxia in late pregnancy causes changes in the biochemical composition of AF; these changes are characterized by high lactate concentrations, and the fetus and uterus can be the source of increased lactate level in AF.

Microcirculatory effects of norepinephrine in patients with septic shock: a microdialysis study

2020 ◽  
Author(s):  
Amira Fatnassi ◽  
Chihebeddine Romdhani ◽  
Widd Kaabi ◽  
Iheb Labbene ◽  
Zied Hajjej ◽  
...  
Keyword(s):  
Septic Shock ◽  
Lactate Concentration ◽  
Inverse Correlation ◽  
Tissue Perfusion ◽  
Control Group ◽  
Adrenergic Drug ◽  
Microdialysis Study ◽  
And Control ◽  
Stable Flow ◽  
Pyruvate Ratio

Abstract Background: The management of septic shock requires the administration of an alpha-adrenergic drug such as norepinephrine, after optimization of the patient’s preload, to maintain adequate mean arterial pressure. Nevertheless, with optimal macrocirculatory parameters, alterations of tissue perfusion can occur. This study aimed to investigate the effect of norepinephrine dosage on microcirculation parameters, studied by microdialysis, in patients with septic shock. Methods: We conducted a retrospective study. We included all patients aged over 16 years in septic shock. We studied three groups (levosimendan, dobutamine, and control group). We administrated norepinephrine before inclusion, at stable flow for more than an hour. We performed hemodynamic monitoring of macrocirculation by echocardiography. We analyzed microcirculation parameters (lactate, pyruvate, and lactate/pyruvate ratio) every six hours during the first three days, by muscle microdialysis (CMA 600, CMA microdialysis AB, Stockholm, Sweden). We studied correlations between microcirculation parameters and norepinephrine doses.Results: We included thirty patients in the study (ten patients in each group). Demographic characteristics and mortality were comparable across the three groups. In total, we analyzed 390 samples of interstitial muscle fluid. We did not find any correlation between norepinephrine doses and the lactate concentration in the muscle, as well as the ratio of lactate/ pyruvate concentration in the muscle (p > 0.05) for all groups. We found a weak inverse correlation between norepinephrine doses and muscle pyruvate levels (p < 0.05) for the dobutamine group and the control group and but not for the levosimendan group.Conclusions: Noradrenaline dose has little effect on microcirculation when administered for hemodynamic optimization, as recommended by the Surviving Sepsis Campaign.

scholarly journals Platelet count estimation: a prognostic index in pregnancy induced hypertension

2018 ◽  
Vol 7 (2) ◽  
pp. 476
Author(s):  
Aakriti Gupta ◽  
Jyoti Hak ◽  
Isha Sunil ◽  
Amita Gupta
Keyword(s):  
Platelet Count ◽  
Pregnant Women ◽  
Prognostic Index ◽  
Disease Process ◽  
Late Pregnancy ◽  
Control Group ◽  
The Mean ◽  
The Difference ◽  
And Control ◽  
In Pregnancy

Background: Hypertension in pregnancy is one of the serious complications of pregnancy with an incidence of 5% to 7% of all pregnancies, particularly in cases with preeclampsia and eclampsia. Though platelet count during pregnancy is within the normal non pregnant reference values, there is a tendency for the platelet count to fall in late pregnancy. The frequency and intensity of maternal thrombocytopenia varies and is dependent on the intensity of the disease process and duration of PIH syndrome.Methods: A prospective observational study was conducted on 200 pregnant women attending OPD Or Inpatients in the Department of Obstetrics and Gynecology, SMGS Hospital, GMC Jammu from November 2015 to October 2016. Pregnant women beyond 28 weeks gestation of pregnancy are included and divided into two groups i.e. the study and control group. Platelet counts will be done every 4 weeks in controls and weekly in subjects from 28 weeks till delivery.Results: The mean platelet count observed among cases of mild preecclampsia, severe preeclampsia and ecclampsia was 2.26, 1.63 and 0.99 lakh/mm3 respectively. The difference in mean platelet count among cases and controls was statistically significant. The association of platelet count with severity of different categories of PIH was analysed statistically and was highly significant.Conclusions: Platelet count is a very important investigation for the antenatal mother having PIH, as it is directly related to maternal and perinatal outcome. Routine and regular monitoring of platelet count can be included in the routine antenatal checkup among the pregnant women with PIH.

scholarly journals Study of effects of oligohydramnios on perinatal outcome

2020 ◽  
Vol 9 (6) ◽  
pp. 2525
Author(s):  
Aashka M. Mashkaria ◽  
Babulal S. Patel ◽  
Akshay C. Shah ◽  
Shashwat K. Jani ◽  
Vismay B. Patel ◽  
...  
Keyword(s):  
Caesarean Section ◽  
Amniotic Fluid ◽  
Perinatal Outcome ◽  
Stress Test ◽  
Fetal Distress ◽  
Control Group ◽  
Study Group ◽  
Amniotic Fluid Index ◽  
Lower Segment Caesarean Section ◽  
And Control

Background: Oligohydramnios has got a noteworthy influence on perinatal outcome. Hence, early detection and its timely management will aid in curtailing of perinatal morbidity and mortality and leading to decreased operative interventions. Therefore, the present study is conducted to look for the effects of oligohydramnios.Methods: This comparative study was a prospective observational study conducted at study institution. The women were divided into study and control groups based on AFI (amniotic fluid index), 100 cases were selected in each group.Results: Out of the 200 women, included in the present study, 35% of the patients in the study group had non-reactive non-stress test (NST) while in the control group 7% had it. Caesarean section was performed in 58% of cases in the study group as compared to 30% in the control group. Amongst these, Fetal distress was the most common indication for LSCS (lower segment caesarean section). There were no perinatal deaths in this study.Conclusions: Based on this study it has been observed that, amniotic fluid index of ≤5 cm was commonly associated with increased LSCS rates, intrauterine growth restriction, non-reactive NST, and abnormal Doppler velocimetry studies. Therefore, every case of oligohydramnios requires to be assessed meticulously. Prompt detection; timely management and treating the underlying condition improve outcome.

scholarly journals Diagnostic capabilities of the biochemical composition of amniotic fluid in assessing fetal conditions

2021 ◽  
Vol 102 (5) ◽  
pp. 773-777
Author(s):  
I B Fatkullina ◽  
A Yu Lazareva ◽  
Yu N Fatkullina ◽  
L A Faizullina ◽  
N A Stecenko ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Biochemical Composition ◽  
Statistical Significance ◽  
Fetal Distress ◽  
Main Group ◽  
Control Group ◽  
Gamma Glutamyl Transpeptidase ◽  
Exact Test ◽  
Nitrogenous Substances ◽  
Diagnostic Capabilities

Aim. To analyze the nature of changes in the biochemical parameters of amniotic fluid during fetal hypoxia. Methods. The study was carried out in the maternity ward of the Republican Clinical Hospital named after Kuvatov (Ufa) between January 2016 and September 2018. The main group 72 women in labor with symptoms of fetal distress, the control group 70 women in labor without it. The biochemical composition of the amniotic fluid was measured using an analyzer. Statistical analysis was performed by using the Statistica 10.0 software. Comparison of qualitative characteristics was carried out by using Fisher's exact test when comparing quantitative data, the MannWhitney test. The statistical significance of the differences was set at p 0.05. Results. It was found that in fetal distress, there is a decrease in such biochemical indicators of the composition of amniotic fluid as the level of triglycerides (0.20.1 and 0.30.1 mmol/L, p=0.0036) and cholesterol (0.10.16 and 0.30.2 mmol/L, p=0.0275), gamma-glutamyl transpeptidase activity (34.511 and 48.76.8 U/L, p=0.0261), while the level the lactate (in the main group 3.51.2 and 3.10.9 mmol/L in the control group, p=0.0035), glucose (1.20.6 and 0.60.3 mmol/L, p=0.0002) and nitrogenous substances such as urea (4.51.1 and 3.01.3 mmol/L, p=0.0018) increases. Conclusion. The biochemical composition of amniotic fluid reflects the state of the fetus at birth, and therefore the study of the amniotic fluid is a relevant and accessible method.

scholarly journals Neonatal Hypoglycemia Related to Glycine Levels in Uncontrolled Gestational Diabetes Mellitus during Mid-Late Pregnancy: Multicenter, Prospective Case-Cohort Observational Study

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Yuhang Long ◽  
Le Chen ◽  
Yaochao Yang ◽  
Wei Liu ◽  
Hanxi Zhang ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Cord Blood ◽  
Late Pregnancy ◽  
Glycosylated Haemoglobin ◽  
Late Gestation ◽  
Control Group ◽  
Neonatal Hypoglycemia ◽  
And Control

Aims. To explore the relationship between gestational diabetes mellitus (GDM) and neonatal cord blood amino acid and carnitine levels after GDM was diagnosed among pregnant women monitoring glycosylated haemoglobin levels of 5.5%-6.4% during mid-late gestation. Methods. In all, 7289 qualified participants were recruited and divided into two groups (GDM and control groups) between 1 July 2015 and 1 July 2020, and all maternal-neonatal data were collected and analyzed at three centers. Results. Interestingly, glycine in cord blood was not only significantly different between groups (15.52 vs. 6.67, P < 0.001 ) but also associated with neonatal hypoglycemia ( r = 0.132 , P < 0.001 ). Although glycine was an independent positive factor with neonatal hypoglycemia, it had lacked effective size to predict the risk of neonatal hypoglycemia ( b = 0.002 , P < 0.001 ). Conclusion. The study identifies some differences and relationships in maternal-neonatal data when the GDM group has fluctuating glycosylated haemoglobin levels of 5.5%-6.4% without hypoglycemic drug intervention, compared with the control group. Although umbilical cord blood of glycine levels has a lack of effective power to predict the risk of neonatal hypoglycemia, it is probably an independent factor involved in the maternal-neonatal glucolipid metabolism.

scholarly journals Quality of life in pregnant women with epilepsy versus women with epilepsy

2011 ◽  
Vol 69 (2b) ◽  
pp. 336-341 ◽  
Author(s):  
L L Lunardi ◽  
A L C Costa ◽  
C A M Guerreiro ◽  
E A P Souza
Keyword(s):  
Quality Of Life ◽  
Pregnant Women ◽  
Post Partum ◽  
Late Pregnancy ◽  
Control Group ◽  
Patients With Epilepsy ◽  
And Control ◽  
Experimental Group

It is assumed that 25% of patients with epilepsy are women of fertile age and 0.3% to 0.6% of all children are born of mothers with epilepsy. The aim of this study was to evaluate the quality of life on pregnant with epilepsy and compare with non-pregnant women with epilepsy. We evaluated two groups (Experimental Group - 29 pregnant women with epilepsy and Control Group - 30 women with epilepsy); they were attended at the HC/UNICAMP. The patients had three meetings to carry out and implement the anamnesis and the application of QQV-65. There were no significant differences in the measurement of quality of life when comparing both groups. However, when we analyzed individually in the pre- and post-partum periods, we observed significant differences in health aspects (p=0.0495), physical (p=0.02868) and emotional (p=0.0253) dimensions in QQV-65. This study shows that pregnancy could be interpreted as a stressor. In late pregnancy when this stressor was removed, women with epilepsy had improvement in their quality of life.

scholarly journals Relevance of lactate level detection in migrane and fibromyalgia

2019 ◽  
Vol 29 (2) ◽  
Author(s):  
Patrizia Proia ◽  
Alessandra Amato ◽  
Valentina Contrò ◽  
Alessandra Lo Monaco ◽  
Jessica Brusa ◽  
...  
Keyword(s):  
Postural Stability ◽  
Path Length ◽  
Lactate Concentration ◽  
Control Group ◽  
Eyes Closed ◽  
Dynamic Coordination ◽  
Eyes Open ◽  
Lactate Levels ◽  
And Control ◽  
Blood Lactate Levels

The aim of this study was to determine the blood lactate levels in healthy and pathological subjects, particularly with migraine and fibromyalgia. Moreover we investigated the possible correlation between lactate concentration, postural stability and balance disorders; the composition of the groups were: migraine (n = 25; age 49.7 ± 12.5), fibromyalgia (n = 10; age 43.7 ± 21.2), control group (n = 16 age 28.52 ± 2.4). The results showed that patients with fibromyalgia (FG) had higher lactate levels compared to migraine (MG) and control group (CG) (mean ± sd: FG = 1.78 ± 0.9 mmol/L; MG = 1.45±1 mmol/L; CG = 0,85 ± 0,07 mmol/L). The same situation was highlighted about the sway path length with eyes closed (FG = 518 ± 195 mm; MG = 465 ± 165 mm; CG = 405 ± 94,72 mm) and with eyes open (FG = 430 ± 220 mm; MG = 411 ± 143 mm; CG = 389 ± 107 mm). This can be explained by the fact that energy-intensive postural strategies must be used to optimize both static and dynamic coordination, in particular with repeated contractions of tonic oxidative muscle cells responsible for postural control.

scholarly journals The effect of social Nervous (SaSo) exercise with insulin resistance (HOMA-IR) on gestational diabetes mellitus among Indonesian pregnant women: a quasi-experimental study

2021 ◽  
Author(s):  
Effatul Afifah ◽  
Detty Siti Nurdiati ◽  
Hamam Hadi ◽  
Zainal Muttaqien Sofro ◽  
Ahmad Hamim Sadewa ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Insulin Resistance ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Exercise Program ◽  
Late Pregnancy ◽  
Control Group ◽  
Quasi Experimental ◽  
And Control ◽  
Experimental Group

Abstract Background Gestational diabetes mellitus (GDM) is a carbohydrate intolerance condition that is diagnosed for the first-time during pregnancy and is associated with various adverse pregnancy outcomes for both mother and child, such as increased rates of perinatal complications and long-term morbidity. Exercise is a strategy to reduce hyperglycemia experienced during gestational diabetes mellitus. One type of exercise that can be done is a social nervous exercise (SaSo). SaSo can stimulate the parasympathetic or myelinated vagus nerves and control blood glucose by stimulating autonomic nerves system so that nerve homeostasis and glucose homeostasis occur. This study aimed to determine the impact of a SaSo program consisting of warm-up, core (prayer movements) and cool-down exercises on glucose homeostasis (HOMA-IR) parameters in women with GDM. Methods The study used a quasi-experimental design. Thirty-seven women with GDM at 24-28 weeks gestation were divided into an experimental group (n=19) with a regularly supervised SaSo program (n=18). The control group received only standard antenatal care for GDM. The exercise program started from the time of diagnosis of diabetes to six weeks of intervention conducted twice per week with sessions lasting 40-45 minutes. Bivariate analysis was used to test the difference in means with pretest and posttest results. Results Majority of pregnant women were in the not at-risk age category for control and intervention groups (79% and 83%, respectively). The baseline data results for the experimental and control groups were homogeneous, with no difference in baseline variables (P>0.05). The social nervous exercise experimental group had lower mean difference (MD±SD) levels of insulin resistence in late pregnancy compared to the control group (-9.15±10.06 vs 3.42 ±14.84) (P=0.004). Conclusions A social nervous exercise program has a beneficial effect on insulin resistance (HOMA-IR) levels in late pregnancy. Further research needs to be done with larger studies to confirm the findings of this study. Trial registration: Ethical approval was obtained from the Medical and Health Research Ethics Committee of the Faculty of Medicine, Universitas Gadjah Mada Yogyakarta (KE/0978/08/2019).

scholarly journals The Protective Effect of Para-Aminobenzoic Acid in HypoxiaInduced Apoptosis of Conjunctiva and Cornea Cells in vivo

2021 ◽  
Vol 18 (2) ◽  
pp. 317-324
Author(s):  
A. A. Ryabtseva ◽  
S. I. Akberova ◽  
G. Kh. Ali-zade ◽  
H. F. Babayev ◽  
Yu. V. Markitantova
Keyword(s):  
Corneal Epithelium ◽  
Hypoxic Hypoxia ◽  
Control Group ◽  
Aminobenzoic Acid ◽  
Adult Rats ◽  
Group Iii ◽  
Group I ◽  
Acute Hypoxic Hypoxia ◽  
Group Ii

Purpose: to evaluate the effects of acute hypoxic hypoxia on eye tissue cells in adult rats and determining the effectiveness of 0.007 % solution of para-aminobenzoic acid (PABA) for apoptotic damage to the tissues of the ocular surface in rats.Materials and methods. In the experiment, 27 male Wistar rats (54 eyes) were used, divided into 5 groups. Group I — intact control, Group II — hypoxic control after 1 hour, Group III — hypoxic control after 3 hours, Group IV — with the injection of the drug 1 hour after hypoxia, Group V — injection of the drug 24 hours before hypoxia. The identification of apoptotic cells in the tissues of the eye was performed by the TUNEL method on frozen sections of the eye stained with Hoechst 33342 nuclear fluorescent dye. The localization and fluorescence intensity of the damaged cells was analyzed under a fluorescence microscope using the Image J. computer program.Results. Under the conditions of simulated acute hypoxic hypoxia, apoptotic lesions of the conjunctiva, corneal epithelium, choroid, and photoreceptor layer of the retina were observed. In group II, the number of damaged cells in the conjunctiva was 67 %, and in group III it was 120 % more compared to group I — the norm (p < 0.05). With the injection of PABA before hypoxia, there were no significant differences compared with the norm (group I) in the state of the affected cells in the conjunctiva. In the group of animals (group III) with the injection of PABA after hypoxia after 1 h, the number of damaged cells does not statistically differ from group II (hypoxic control after 1 h), but significantly lower than in group III (hypoxic control after 3 hours). The same pattern is observed in the corneal epithelium.Conclusion. Para-aminobenzoic acid (0.007 %) has a prophylactic and therapeutic effect, preventing and stabilizing the development of apoptosis of conjunctival cells and anterior corneal epithelium induced in experimental acute hypoxic hypoxia. 

scholarly journals BIOCHEMICAL COMPOSITION OF THE BLOOD OF DOGS ON THE BACKGROUND OF THE INFLUENCE OF INTENSIVE INVASION OF DIROFILARIASIS

2019 ◽  
pp. 132-135
Author(s):  
Bittirova ◽  
Kabardiev ◽  
Bittirov ◽  
Kabardiev ◽  
Begieva ◽  
...  
Keyword(s):  
Biochemical Composition ◽  
Total Bilirubin ◽  
Blood Parameters ◽  
Control Group ◽  
Pathological Effect ◽  
Blood Tests ◽  
Biochemical Studies ◽  
And Control ◽  
Experimental Group ◽  
Forearm Vein

Experimentally obtained new data in the study of the biochemical composition of blood of invasive dogs on the background of the influence of intensive invasion of dirofilariоsis. Biochemical blood tests were performed on 20 dogs intensively infected with microfilaria pathogens of dirofilariasis using standard methods. In the morning, blood from dogs was taken from the forearm vein in test tubes with an anticoagulant for a general blood test, in test tubes with a clotting activator – for biochemical studies. For the study were formed 2 groups: experimental and control. The experimental group (n = 10) consisted of dogs that had microfilariae of dirofilariоsis pathogens in blood samples. A control group of dogs (n = 10) was formed from nonhelminthic animals. Intensive dirofilariasis invasion has a pathological effect on the biochemical blood parameters of dogs in the form of an increase in total whey protein by 28.83 g / l, and enzymes: AST by 27.89 units / l and ALT 47.95 units / l, total bilirubin in 38.01 μmol / l and urea in 17.07 μmol / l. Against the background of control at high intensity of dirofilariasis invasion in dogs, hyperproteinemia with a multiple increase in the activity of AST and ALT, bilirubin and urea manifest with firmness.

Sign in / Sign up

Export Citation Format

Share Document