scholarly journals Microbial Decontamination by Pulsed Electric Fields (PEF) in Winemaking

2021 ◽  
Author(s):  
Carlota Delso ◽  
Alejandro Berzosa ◽  
Jorge Sanz ◽  
Ignacio Álvarez ◽  
Javier Raso

Pulsed Electric Fields (PEF) is a non-thermal technique that causes electroporation of cell membranes by applying very short pulses (μs) of a high-intensity electric field (kV/cm). Irreversible electroporation leads to the formation of permanent conductive channels in the cytoplasmic membrane of cells, resulting in the loss of cell viability. This effect is achieved with low energy requirements and minimal deterioration of quality. This chapter reviews the studies hitherto conducted to evaluate the potential of PEF as a technology for microbial decontamination in the winemaking process for reducing or replacing the use of SO2, for guaranteeing reproducible fermentations or for wine stabilization.

2003 ◽  
Vol 17 (11) ◽  
pp. 1-23 ◽  
Author(s):  
Stephen J. Beebe ◽  
Paula M. Fox ◽  
Laura J. Rec ◽  
Lauren K. Willis ◽  
Karl H. Schoenbach

2020 ◽  
Vol 246 (3) ◽  
pp. 539-548
Author(s):  
Rogelio Sánchez-Vega ◽  
Teresa Garde-Cerdán ◽  
María Janeth Rodríguez-Roque ◽  
Pedro Elez-Martínez ◽  
Olga Martín-Belloso

Cancers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1132 ◽  
Author(s):  
Philip M. Graybill ◽  
Rafael V. Davalos

Pulsed electric fields (PEFs) have become clinically important through the success of Irreversible Electroporation (IRE), Electrochemotherapy (ECT), and nanosecond PEFs (nsPEFs) for the treatment of tumors. PEFs increase the permeability of cell membranes, a phenomenon known as electroporation. In addition to well-known membrane effects, PEFs can cause profound cytoskeletal disruption. In this review, we summarize the current understanding of cytoskeletal disruption after PEFs. Compiling available studies, we describe PEF-induced cytoskeletal disruption and possible mechanisms of disruption. Additionally, we consider how cytoskeletal alterations contribute to cell–cell and cell–substrate disruption. We conclude with a discussion of cytoskeletal disruption-induced anti-vascular effects of PEFs and consider how a better understanding of cytoskeletal disruption after PEFs may lead to more effective therapies.


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