scholarly journals Cytoskeletal Disruption after Electroporation and Its Significance to Pulsed Electric Field Therapies

Cancers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1132 ◽  
Author(s):  
Philip M. Graybill ◽  
Rafael V. Davalos
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Irreversible Electroporation ◽  
Pulsed Electric Fields ◽  
Pulsed Electric Field ◽  
Current Understanding ◽  
Vascular Effects ◽  
Cell Substrate ◽  
Membrane Effects ◽  
Cytoskeletal Disruption

Pulsed electric fields (PEFs) have become clinically important through the success of Irreversible Electroporation (IRE), Electrochemotherapy (ECT), and nanosecond PEFs (nsPEFs) for the treatment of tumors. PEFs increase the permeability of cell membranes, a phenomenon known as electroporation. In addition to well-known membrane effects, PEFs can cause profound cytoskeletal disruption. In this review, we summarize the current understanding of cytoskeletal disruption after PEFs. Compiling available studies, we describe PEF-induced cytoskeletal disruption and possible mechanisms of disruption. Additionally, we consider how cytoskeletal alterations contribute to cell–cell and cell–substrate disruption. We conclude with a discussion of cytoskeletal disruption-induced anti-vascular effects of PEFs and consider how a better understanding of cytoskeletal disruption after PEFs may lead to more effective therapies.

Pulsed Electric Fields Versus Thermal Treatment: Equivalent Processes To Obtain Equally Acceptable Citrus Juices

2006 ◽  
Vol 69 (8) ◽  
pp. 2016-2018 ◽  
Author(s):  
E. SENTANDREU ◽  
L. CARBONELL ◽  
D. RODRIGO ◽  
J. V. CARBONELL
Keyword(s):  
Thermal Treatment ◽  
Electric Field ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Pectin Methylesterase ◽  
Pulsed Electric Field ◽  
Citrus Juices ◽  
Pulsed Electric Field Treatment ◽  
Electric Field Treatment

Pulsed electric field treatment has been claimed to produce more acceptable chilled citrus juices than those obtained by conventional thermal treatment. The pectin methylesterase activity and the acceptability of nine juices obtained from Clementine mandarins, Valencia oranges, and Ortanique fruits (hybrid of mandarin and orange), untreated, pasteurized (85°C for 10 s), and treated by pulsed electric fields (25 kV/cm for 330 μs), were evaluated. The treatments, selected to reach a similar level of pectin methylesterase inactivation, produced juices that did not differ in acceptability from each other for the three varieties and in all cases were less acceptable than the untreated juice.

scholarly journals Towards Solid Tumor Treatment by Nanosecond Pulsed Electric Fields

2009 ◽  
Vol 8 (4) ◽  
pp. 289-306 ◽  
Author(s):  
Axel T. Esser ◽  
Kyle C. Smith ◽  
T. R. Gowrishankar ◽  
James C. Weaver
Keyword(s):  
Cell Death ◽  
Electric Field ◽  
Spatial Scale ◽  
Electric Fields ◽  
Solid Tumor ◽  
Irreversible Electroporation ◽  
Pulsed Electric Fields ◽  
Underlying Mechanism ◽  
Tumor Ablation ◽  
Nanosecond Pulsed Electric Fields

Local and drug-free solid tumor ablation by large nanosecond pulsed electric fields leads to supra-electroporation of all cellular membranes and has been observed to trigger nonthermal cell death by apoptosis. To establish pore-based effects as the underlying mechanism inducing apoptosis, we use a multicellular system model (spatial scale 100 μm) that has irregularly shaped liver cells and a multiscale liver tissue model (spatial scale 200 mm). Pore histograms for the multicellular model demonstrate the presence of only nanometer-sized pores due to nanosecond electric field pulses. The number of pores in the plasma membrane is such that the average tissue conductance during nanosecond electric field pulses is even higher than for longer irreversible electroporation pulses. It is shown, however, that these nanometer-sized pores, although numerous, only significantly change the permeability of the cellular membranes to small ions, but not to larger molecules. Tumor ablation by nanosecond pulsed electric fields causes small to moderate temperature increases. Thus, the underlying mechanism(s) that trigger cell death by apoptosis must be non-thermal electrical interactions, presumably leading to different ionic and molecular transport than for much longer irreversible electroporation pulses.

Impact of high-intensity pulsed electric fields on carotenoids profile of tomato juice made of moderate-intensity pulsed electric field-treated tomatoes

2013 ◽  
Vol 141 (3) ◽  
pp. 3131-3138 ◽  
Author(s):  
Anna Vallverdú-Queralt ◽  
Isabel Odriozola-Serrano ◽  
Gemma Oms-Oliu ◽  
Rosa M Lamuela-Raventós ◽  
Pedro Elez-Martínez ◽  
...  
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
High Intensity ◽  
Pulsed Electric Fields ◽  
Pulsed Electric Field ◽  
Tomato Juice ◽  
Moderate Intensity

scholarly journals Pulsed Electric Fields Alter Expression of NF-κB Promoter-Controlled Gene

2021 ◽  
Vol 23 (1) ◽  
pp. 451
Author(s):  
Justina Kavaliauskaitė ◽  
Auksė Kazlauskaitė ◽  
Juozas Rimantas Lazutka ◽  
Gatis Mozolevskis ◽  
Arūnas Stirkė
Keyword(s):  
Gene Expression ◽  
Synthetic Biology ◽  
Electric Field ◽  
Electric Fields ◽  
Mammalian Cells ◽  
Single Gene ◽  
Pulsed Electric Fields ◽  
Pulsed Electric Field ◽  
Potential Candidate ◽  
Cell Parameters

The possibility to artificially adjust and fine-tune gene expression is one of the key milestones in bioengineering, synthetic biology, and advanced medicine. Since the effects of proteins or other transgene products depend on the dosage, controlled gene expression is required for any applications, where even slight fluctuations of the transgene product impact its function or other critical cell parameters. In this context, physical techniques demonstrate optimistic perspectives, and pulsed electric field technology is a potential candidate for a noninvasive, biophysical gene regulator, exploiting an easily adjustable pulse generating device. We exposed mammalian cells, transfected with a NF-κB pathway-controlled transcription system, to a range of microsecond-duration pulsed electric field parameters. To prevent toxicity, we used protocols that would generate relatively mild physical stimulation. The present study, for the first time, proves the principle that microsecond-duration pulsed electric fields can alter single-gene expression in plasmid context in mammalian cells without significant damage to cell integrity or viability. Gene expression might be upregulated or downregulated depending on the cell line and parameters applied. This noninvasive, ligand-, cofactor-, nanoparticle-free approach enables easily controlled direct electrostimulation of the construct carrying the gene of interest; the discovery may contribute towards the path of simplification of the complexity of physical systems in gene regulation and create further synergies between electronics, synthetic biology, and medicine.

scholarly journals Modelling of Carotenoids Content in Red Clover Sprouts Using Light of Different Wavelength and Pulsed Electric Field

2020 ◽  
Vol 10 (12) ◽  
pp. 4143
Author(s):  
Ilona Gałązka-Czarnecka ◽  
Ewa Korzeniewska ◽  
Andrzej Czarnecki ◽  
Paweł Kiełbasa ◽  
Tomasz Dróżdż
Keyword(s):  
Electric Field ◽  
Blue Light ◽  
Electric Fields ◽  
White Light ◽  
Light Exposure ◽  
Red Clover ◽  
Pulsed Electric Fields ◽  
Pulsed Electric Field ◽  
Pre Treatment ◽  
Β Carotene

The paper presents the results of influence the light of different wavelengths and pulsed electric fields on the content of carotenoids. Seeds germination was carried out in a climatic chamber with phytotron system. The experiment was carried out under seven growing conditions differing in light-emitting diode (LED) wavelengths and using pulsed electric fields (PEFs) with different strength applied before sowing. Cultivation of the sprouts was carried out for seven days at relative humidity 80% and 20 ± 1 °C. Different light wavelengths were used during cultivation: white light (380–780 nm), UVA (340 nm), blue (440 nm), and red (630 nm). In addition, the pulsed electric field (PEF) with three values of strength equal to 1, 2.5 and 5 kV/cm, respectively, was applied to three series of sprouts before sowing. Sprouts treated with the PEF were grown under white light (380–780 nm). The light exposure time for all experimental series of sprouts was 12/12 h (12 h light, 12 h dark for seven days). Lutein is the dominant carotenoid in germinating red clover seeds, the content of which varies from 743 mg/kg in sprouts grown in red light, 862 mg/kg in sprouts grown in UVA, to 888 mg/kg in sprouts grown in blue light. Blue light in the cultivation of red clover sprouts had the most beneficial effect on the increase of carotenoids content and amounted to 42% in β-carotene, 19% in lutein, and 14% in zeaxanthin. It confirms that modelling the content of carotenoids is possible using UVA and blue light (440 nm) during seed cultivation. An increase in the content of β-carotene and lutein in red clover sprouts was obtained in comparison to the test with white light without PEF pre-treatment, respectively by 8.5% and 6%. At the same time a 3.3% decrease in the content of zeaxanthin was observed. Therefore, it can be concluded that PEF pre-treatment may increase mainly the content of β-carotene.

scholarly journals Integrating flexible electronics for pulsed electric field delivery in a vascularized 3D glioblastoma model

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Marie C. Lefevre ◽  
Gerwin Dijk ◽  
Attila Kaszas ◽  
Martin Baca ◽  
David Moreau ◽  
...  
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Flexible Electronics ◽  
Soft Tissues ◽  
Multiphoton Microscopy ◽  
Membrane Integrity ◽  
Tumor Model ◽  
Pulsed Electric Fields ◽  
In Ovo ◽  
Cell Membrane Integrity

AbstractGlioblastoma is a highly aggressive brain tumor, very invasive and thus difficult to eradicate with standard oncology therapies. Bioelectric treatments based on pulsed electric fields have proven to be a successful method to treat cancerous tissues. However, they rely on stiff electrodes, which cause acute and chronic injuries, especially in soft tissues like the brain. Here we demonstrate the feasibility of delivering pulsed electric fields with flexible electronics using an in ovo vascularized tumor model. We show with fluorescence widefield and multiphoton microscopy that pulsed electric fields induce vasoconstriction of blood vessels and evoke calcium signals in vascularized glioblastoma spheroids stably expressing a genetically encoded fluorescence reporter. Simulations of the electric field delivery are compared with the measured influence of electric field effects on cell membrane integrity in exposed tumor cells. Our results confirm the feasibility of flexible electronics as a means of delivering intense pulsed electric fields to tumors in an intravital 3D vascularized model of human glioblastoma.

Chemical Analysis on the Effect of Pulsed Electric Fields in Pineapple Juices Preservation

2014 ◽  
Vol 554 ◽  
pp. 588-592 ◽  
Author(s):  
Ali Mohammad Dastgheib ◽  
Zolkafle Buntat ◽  
Muhammad Abu Bakar Sidik
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Food Industry ◽  
Pulsed Electric Field ◽  
Liquid Food ◽  
Chemical Parameters ◽  
Natural Properties ◽  
Viable Solution ◽  
Before And After ◽  
Thermal Technique

The application of high voltage electric field for preservation of fruit juices has a promising scope in the food industry. The pulsed electric field (PEF) is an innovative non- thermal technique and free from bio-toxic effects. The technique has a viable solution of the problem yet faced in the food industry to prolong life and preserve and maintain quality with natural properties of the liquid food and beverages. In this study, we have treated the pineapples juice samples by different strengths of pulsed electric field such as 10, 20 and 30kV/cm for 5 minutes in each test. This study used new design of helix treatment chamber with three different lengths of 20, 30and 50cm. In these experiments, all samples were kept in same and normal condition with a temperature around 25-26 andthe humidity was between 55 and 65%. Then the observation based on chemical tests such as pH, conductivity, salinity and total dissolved solids (TDS) was recorded for all samples before and after the test. Based on results obtained by chemical parameters suggest that the injection on pulsed electric field of 30 kV/cm by the 50 cm treatment chamber has the best effect on pineapple juices characteristic as compared to the other value. The result of this experiment is encouraging and supportive of the better way for pasteurization the pineapple juices and increasing longevity of pineapple juices.

Critical Electric Field Strengths of Onion Tissues Treated by Pulsed Electric Fields

2010 ◽  
Vol 75 (7) ◽  
pp. E433-E443 ◽  
Author(s):  
Suvaluk Asavasanti ◽  
Seda Ersus ◽  
William Ristenpart ◽  
Pieter Stroeve ◽  
Diane M. Barrett
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Field Strengths

scholarly journals Review of the Dynamics of Coalescence and Demulsification by High-Voltage Pulsed Electric Fields

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Ye Peng ◽  
Tao Liu ◽  
Haifeng Gong ◽  
Xianming Zhang
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
High Voltage ◽  
Industrial Application ◽  
Voltage Pulse ◽  
Pulse Electric Field ◽  
Pulsed Electric Fields ◽  
High Voltage Pulse ◽  
Technological Method ◽  
Pulse Electric

The coalescence of droplets in oil can be implemented rapidly by high-voltage pulse electric field, which is an effective demulsification dehydration technological method. At present, it is widely believed that the main reason of pulse electric field promoting droplets coalescence is the dipole coalescence and oscillation coalescence in pulse electric field, and the optimal coalescence pulse electric field parameters exist. Around the above content, the dynamics of high-voltage pulse electric field promoting the coalescence of emulsified droplets is studied by researchers domestically and abroad. By review, the progress of high-voltage pulse electric field demulsification technology can get a better understanding, which has an effect of throwing a sprat to catch a whale on promoting the industrial application.

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