scholarly journals Bibliographic Review of the Application of Ovulation Synchronization Protocol Based on Gonadotropin-Releasing Hormone (GnRH) and Insulin to Increase the Conception Rate in Crossbred Holstein Cows

Estrogen ◽  
2019 ◽  
Author(s):  
Calderón-Luna Joselin ◽  
Santos-Calderón Carlos
Keyword(s):  
Gonadotropin Releasing Hormone ◽  
Conception Rate ◽  
Holstein Cows ◽  
Releasing Hormone ◽  
Synchronization Protocol

Two novel SNPs of the type I gonadotropin releasing hormone receptor gene and their associations with superovulation traits in Chinese Holstein cows

2011 ◽  
Vol 136 (2-3) ◽  
pp. 164-168 ◽  
Author(s):  
Wu-Cai Yang ◽  
Shu-Jing Li ◽  
Yang-Hua Xie ◽  
Ke-Qiong Tang ◽  
Guo-Hua Hua ◽  
...  
Keyword(s):  
Hormone Receptor ◽  
Receptor Gene ◽  
Gonadotropin Releasing Hormone ◽  
Holstein Cows ◽  
Type I ◽  
Releasing Hormone ◽  
Chinese Holstein ◽  
Chinese Holstein Cows ◽  
Gonadotropin Releasing Hormone Receptor ◽  
Hormone Receptor Gene

408 HOLSTEIN DONORS RESPONSE USING OR NOT USING ESTRADIOL 17-β IN THE SUPEROVULATORY TREATMENT

2010 ◽  
Vol 22 (1) ◽  
pp. 361
Author(s):  
S. Fuentes ◽  
D. Ruiz ◽  
P. Vera ◽  
A. Castro ◽  
I. Moreno ◽  
...  
Keyword(s):  
Treatment Protocol ◽  
Gonadotropin Releasing Hormone ◽  
Holstein Cows ◽  
The European Union ◽  
Treatment Protocols ◽  
Releasing Hormone ◽  
Lactating Cows ◽  
Group A ◽  
Group B ◽  
Rectal Palpation

Because of new regulations banning the use of estradiol in the European Union and other countries, the present study was designed to determine the efficacy of an alternative to the use of estradiol 17-β in superovulation of Holstein cows and heifers. A total of 61 lactating cows and 49 heifers (15-18 months old) were divided into 2 groups: Group A (32 cows and 22 heifers) and Group B (29 cows and 27 heifers). Animals in Group A received a CIDR device (Pfizer, Madrid, Spain) for 13 days. At the same time of CIDR insertion and 5 days later, all animals received 2 mL (0.15 mg) of d-cloprostenol (PGF; Veteglan®, Calier, Spain). Gonadotropin-releasing hormone (0.5 mg of gonadorelin; Fertagyl®, Schering-Plough, Carbajosa de la Sagrada, Spain) was administered twice, 36 h after the second PGF and 24 h after CIDR removal. Superovulation treatments were initiated 36 h after the first GnRH, with a total dose of 15 mL (750 IU) of FSH (Pluset®, Calier, Spain) for cows and 11.5 mL (575 IU) for heifers, given in 10 twice-daily injections that decreased in dose. Luteolysis was induced with 0.15 mg of PGF given twice, 12 h before and also at the time of CIDR removal. Animals in Group B received a CIDR device for 7 days. Twenty-four hours after CIDR insertion, all animals received 100 mg of progesterone (Smithkline, Tres Cantos, Spain) and 5 mg of estradiol 17-β (Sigma, St. Louis, MO, USA). Beginning on Day 5, animals were superovulated with similar doses of FSH to those used in Group A. Luteolysis was induced with 0.15 mg of PGF given twice, 12 h before CIDR removal and also at the time of removal. Gonadotropin-releasing hormone (0.5 mg) was administered at the time of AI. Animals from both groups were inseminated with frozen-thawed semen at 12 and 24 h after the onset of standing estrus. Embryos were recovered nonsurgically 7 days later. A total of 299 frozen-thawed embryos (Freeze Control® CL5500, Bioniche, Belleville, Ontario, Canada) from both groups were transferred in synchronized Holstein heifers. Pregnancy was confirmed by rectal palpation on Day 40 to 45 of gestation. Data were analyzed by two-way ANOVA and results are shown in the table. No significant differences were found between cows and heifers, nor was there a difference between groups. These results showed that the previous superovulatory treatment using progesterone and estradiol can be successfully replaced with the treatment protocol described herein. Table 1.Mean number of recovered ova/embryos, transferable embryos, and pregnancy rates after embryo transfer (ET) in Holstein cows and heifers superovulated with 2 treatment protocols

scholarly journals Pattern of induced estrus and conception rate following Ovsynch and Ovsynch based gonadotropin-releasing hormone treatments initiated on day 6 of estrous cycle in repeat breeding crossbred cows

2016 ◽  
Vol 9 (04) ◽  
pp. 342-345 ◽  
Author(s):  
N. Ahmed ◽  
D. Kathiresan ◽  
F. A. Ahmed ◽  
K. Lalrintluanga ◽  
P. Mayengbam ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Gonadotropin Releasing Hormone ◽  
Conception Rate ◽  
Releasing Hormone ◽  
Crossbred Cows

scholarly journals GPGMH, a New Fixed Timed-AI Synchronization Regimen for Swamp and River Crossbred Buffaloes (Bubalus bubalis)

2021 ◽  
Vol 8 ◽  
Author(s):  
Adili Abulaiti ◽  
Hadeel S. El-Qaliouby ◽  
Halla E. K. El Bahgy ◽  
Zahid Naseer ◽  
Zulfiqar Ahmed ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Estradiol Benzoate ◽  
Bubalus Bubalis ◽  
Gonadotropin Releasing Hormone ◽  
Pregnancy Rates ◽  
Releasing Hormone ◽  
Follicular Cyst ◽  
Prostaglandin F ◽  
Estrus Response ◽  
Synchronization Protocol

The crossbreeding of Swamp and River type buffalo breeds is practiced for the improvement of milk yield and reproductive performance in swamp buffalo herds. This study aimed to modify the Ovsynch synchronization protocol (GPG) and improve the fixed-timed artificial insemination (FTAI) for better reproductive performance of crossbred buffaloes. Comparison of four conventional synchronization protocols [pregnant mare gonadotropin-prostaglandin F2α-gonadotropin-releasing hormone (PmPG), gonadotropin-releasing hormone-prostaglandin F2α-gonadotropin-releasing hormone (GPG), prostaglandin F2α-gonadotropin-releasing hormone-prostaglandin F2α-estradiol benzoate (PGPE), and progesterone-pregnant mare gonadotropin-prostaglandin F2α-gonadotropin-releasing hormone (P4PmPG)] in crossbred buffaloes showed that the GPG protocol treated buffaloes displayed higher (P < 0.05) estrus response with an increasing tendency in ovulation (84.6%) and pregnancy rates (30.8%) than PmPG, PGPE, and P4PmPG treated buffaloes. Buffaloes treated with a dose of 0.4 (mg/kg) mifepristone combined with GPG, exhibited higher (P < 0.05) estrous response (82.4%), ovulation (94.1%), and pregnancy (47.1%) rates compared with other doses (0, 0.3, or 0.5 mg/kg) groups. Injection of mifepristone along second GnRH injection in buffaloes improved (P < 0.05) pregnancy rate (35.3%) when compared to before or after the second GnRH of GPG protocol. Single AI after 24 h of mifepristone or second GnRH injection seems the best time to enhance the pregnancy rates in buffaloes compared to double or other single AI times in the modified GPGMH protocol. In comparison, GPGMH reduced the follicular cyst incidence (P < 0.05) with increasing ovulation (P > 0.05) and pregnancy rates (P > 0.05) than the P4GPG and GPG protocols in crossbred buffaloes. The current study supported that new synchronization protocol (modified of GPG protocol; GPGMH) by the inclusion of mifepristone (with a dose of 0.4 mg/kg along second GnRH), AI after 24 h of mifepristone or second GnRH, and human chorionic gonadotropin (hCG at day 5 of AI) enhance the ovulation and pregnancy rates in crossbred buffaloes.

15 Effects of Timed Artificial Insemination Protocol Using Prostglandin F2α and Estradiol Benzoate on Conception Rate in High-Producing Dairy Cows

2018 ◽  
Vol 30 (1) ◽  
pp. 147
Author(s):  
M. Yamaguchi ◽  
M. Takayama ◽  
H. López
Keyword(s):  
Reproductive Performance ◽  
Prostaglandin F2α ◽  
Estradiol Benzoate ◽  
Conception Rate ◽  
Holstein Cows ◽  
Dairy Herds ◽  
Chi Square ◽  
Parity Number ◽  
Chi Square Test ◽  
Synchronization Protocol

For several years, milk production per Holstein cow has been increasing while reproductive performance has declined worldwide. Timed AI (TAI) protocols with synchronization of oestrus and ovulation are tools for the improvement of reproductive performance in high producing dairy herds. In protocols for synchronization of oestrus and ovulation, oestradiol benzoate (EB) has been used to increase the number of animals that show oestrous behaviour, synchronize ovulation, and improve conception rate (CR). The objective of this study was to compare a simple oestrus synchronization and TAI protocol using prostaglandin F2α (PG) and EB with a more complicated TAI protocol or oestrus detection and AI on CR in high-producing Holstein cows. The study was conducted from 2014 to 2016, using 1,036 Holstein cows from 3 commercial dairy herds in central Hokkaido, Japan. The average parity number was 2.6 ± 1.5, and days open averaged 152.2 ± 82.0 days. The average milk yield (305 days) was 11,348 ± 1,619 kg. Oestrus synchronization of cows was achieved using PG followed by EB and TAI (PG was given to cows with a cutoff for CL diameter of >20 mm by ultrasonography; EB (1 mg) was administered 24 h later and AI was carried out 24 to 48 h after EB injection), or by Heatsynch + CIDR [gonadotropin-releasing hormone (GnRH) injection and CIDR insertion at random stages of the oestrous cycle, CIDR removal, and PG injection 7 days later, EB injected 24 h after CIDR removal, and TAI 24 to 32 h after EB injection]. Cows observed in natural oestrus were used as controls. All cows were inseminated after the onset of standing heat or removal of the tail chalk (Paintstik®, LA-CO Industries, Elk Grove Village, IL, USA). Pregnancy diagnoses were performed using ultrasonography between 30 and 45 days after AI. The CR were compared using chi-square test. The CR (no. of pregnant cows/no. of total cows in the treatment group) of the natural oestrus group, PG + EB, and Heatsynch + CIDR cows were 39.2% (302/771), 38.7% (48/124), and 31.2% (44/141), respectively. Overall CR for the 3 groups was 38.0%. There were no significant differences in CR among the 2 groups (P > 0.07). These results show that a simple oestrus synchronization protocol with injection of PG followed by EB can be used effectively for high-producing dairy herds, yielding a satisfactory CR similar to that obtained with the Heatsynch + CIDR protocol or natural oestrus.

scholarly journals Administration of gonadotropin-releasing hormone agonist on Day 5 increases luteal blood flow and improves pregnancy prediction accuracy on Day 14 in recipient Holstein cows

2017 ◽  
Vol 63 (4) ◽  
pp. 389-399 ◽  
Author(s):  
Tomomi KANAZAWA ◽  
Motohide SEKI ◽  
Keiki ISHIYAMA ◽  
Masao ARASEKI ◽  
Yoshiaki IZAIKE ◽  
...  
Keyword(s):  
Blood Flow ◽  
Prediction Accuracy ◽  
Gonadotropin Releasing Hormone ◽  
Holstein Cows ◽  
Releasing Hormone ◽  
Gonadotropin Releasing Hormone Agonist
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