scholarly journals Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

2020 ◽  
Author(s):  
Lucie Bacakova ◽  
Julia Pajorova ◽  
Marketa Zikmundova ◽  
Elena Filova ◽  
Petr Mikes ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Nanofibrous Scaffolds

Nanofibrous scaffolds for skin tissue engineering and wound healing applications

2022 ◽  
pp. 645-681
Author(s):  
Guadalupe Rivero ◽  
Matthäus D. Popov Pereira da Cunha ◽  
Pablo C. Caracciolo ◽  
Gustavo A. Abraham
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Nanofibrous Scaffolds

scholarly journals Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic Polymers

2020 ◽  
Author(s):  
Lucie Bacakova ◽  
Marketa Zikmundova ◽  
Julia Pajorova ◽  
Antonin Broz ◽  
Elena Filova ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Synthetic Polymers ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Nanofibrous Scaffolds

scholarly journals Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering

2019 ◽  
Vol 20 (20) ◽  
pp. 5174 ◽  
Author(s):  
Hariharan Ezhilarasu ◽  
Raghavendra Ramalingam ◽  
Chetna Dhand ◽  
Rajamani Lakshminarayanan ◽  
Asif Sadiq ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Aloe Vera ◽  
Dermal Fibroblasts ◽  
Tetracycline Hydrochloride ◽  
Skin Tissue ◽  
Electrospinning Technique ◽  
Skin Tissue Engineering ◽  
Nanofibrous Scaffolds ◽  
Diffusion Assay

Aloe vera (AV) and tetracycline hydrochloride (TCH) exhibit significant properties such as anti-inflammatory, antioxidant and anti-bacterial activities to facilitate skin tissue engineering. The present study aims to develop poly-ε-caprolactone (PCL)/ AV containing curcumin (CUR), and TCH loaded hybrid nanofibrous scaffolds to validate the synergistic effect on the fibroblast proliferation and antimicrobial activity against Gram-positive and Gram-negative bacteria for wound healing. PCL/AV, PCL/CUR, PCL/AV/CUR and PCL/AV/TCH hybrid nanofibrous mats were fabricated using an electrospinning technique and were characterized for surface morphology, the successful incorporation of active compounds, hydrophilicity and the mechanical property of nanofibers. SEM revealed that there was a decrease in the fiber diameter (ranging from 360 to 770 nm) upon the addition of AV, CUR and TCH in PCL nanofibers, which were randomly oriented with bead free morphology. FTIR spectra of various electrospun samples confirmed the successful incorporation of AV, CUR and TCH into the PCL nanofibers. The fabricated nanofibrous scaffolds possessed mechanical properties within the range of human skin. The biocompatibility of electrospun nanofibrous scaffolds were evaluated on primary human dermal fibroblasts (hDF) by MTS assay, CMFDA, Sirius red and F-actin stainings. The results showed that the fabricated PCL/AV/CUR and PCL/AV/TCH nanofibrous scaffolds were non-toxic and had the potential for wound healing applications. The disc diffusion assay confirmed that the electrospun nanofibrous scaffolds possessed antibacterial activity and provided an effective wound dressing for skin tissue engineering.

scholarly journals Skin tissue engineering: wound healing based on stem-cell-based therapeutic strategies

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Azar Nourian Dehkordi ◽  
Fatemeh Mirahmadi Babaheydari ◽  
Mohammad Chehelgerdi ◽  
Shiva Raeisi Dehkordi
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Stem Cell ◽  
Therapeutic Strategies ◽  
Skin Tissue ◽  
Skin Tissue Engineering

Quercetin promotes human epidermal stem cell proliferation through the estrogen receptor/β-catenin/c-Myc/cyclin A2 signaling pathway

2020 ◽  
Vol 52 (10) ◽  
pp. 1102-1110
Author(s):  
Zhaodong Wang ◽  
Guangliang Zhang ◽  
Yingying Le ◽  
Jihui Ju ◽  
Ping Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Wound Healing ◽  
Estrogen Receptor ◽  
Signaling Pathway ◽  
Skin Wound ◽  
Skin Tissue ◽  
Skin Wound Healing ◽  
Skin Tissue Engineering ◽  
Cyclin A2

Abstract Skin epidermal stem cells (EpSCs) play an important role in wound healing. Quercetin is a phytoestrogen reported to accelerate skin wound healing, but its effect on EpSCs is unknown. In this study, we investigated the effect of quercetin on human EpSC proliferation and explored the underlying mechanisms. We found that quercetin at 0.1~1 μM significantly promoted EpSC proliferation and increased the number of cells in S phase. The pro-proliferative effect of quercetin on EpSCs was confirmed in cultured human skin tissue. Mechanistic studies showed that quercetin significantly upregulated the expressions of β-catenin, c-Myc, and cyclins A2 and E1. Inhibitor for β-catenin or c-Myc significantly inhibited quercetin-induced EpSC proliferation. The β-catenin inhibitor XAV-939 suppressed quercetin-induced expressions of β-catenin, c-Myc, and cyclins A2 and E1. The c-Myc inhibitor 10058-F4 inhibited the upregulation of c-Myc and cyclin A2 by quercetin. Pretreatment of EpSCs with estrogen receptor (ER) antagonist ICI182780, but not the G protein-coupled ER1 antagonist G15, reversed quercetin-induced cell proliferation and upregulation of β-catenin, c-Myc, and cyclin A2. Collectively, these results indicate that quercetin promotes EpSC proliferation through ER-mediated activation of β-catenin/c-Myc/cyclinA2 signaling pathway and ER-independent upregulation of cyclin E1 and that quercetin may accelerate skin wound healing through promoting EpSC proliferation. As EpSCs are used not only in clinic to treat skin wounds but also as seed cells in skin tissue engineering, quercetin is a useful reagent to expand EpSCs for basic research, skin wound treatment, and skin tissue engineering.

Preparation and characterization of polyhydroxybutyrate-co -hydroxyvalerate/silk fibroin nanofibrous scaffolds for skin tissue engineering

2014 ◽  
Vol 55 (4) ◽  
pp. 907-916 ◽  
Author(s):  
Caihong Lei ◽  
Hailin Zhu ◽  
Jingjing Li ◽  
Jiuming Li ◽  
Xinxing Feng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Nanofibrous Scaffolds

Emu oil-based electrospun nanofibrous scaffolds for wound skin tissue engineering

2012 ◽  
Vol 415 ◽  
pp. 454-460 ◽  
Author(s):  
Afeesh R. Unnithan ◽  
P.B. Tirupathi Pichiah ◽  
Gopalsamy Gnanasekaran ◽  
Kalaiselvi Seenivasan ◽  
Nasser A.M. Barakat ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Emu Oil ◽  
Nanofibrous Scaffolds

scholarly journals Immunomodulation of Skin Repair: Cell-Based Therapeutic Strategies for Skin Replacement (A Comprehensive Review)

Biomedicines ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 118
Author(s):  
Shima Tavakoli ◽  
Marta A. Kisiel ◽  
Thomas Biedermann ◽  
Agnes S. Klar
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Wound Healing ◽  
Skin Wound ◽  
Skin Tissue ◽  
Diabetic Patients ◽  
Specific Cell ◽  
Skin Substitutes ◽  
Skin Wound Healing ◽  
Skin Tissue Engineering

The immune system has a crucial role in skin wound healing and the application of specific cell-laden immunomodulating biomaterials emerged as a possible treatment option to drive skin tissue regeneration. Cell-laden tissue-engineered skin substitutes have the ability to activate immune pathways, even in the absence of other immune-stimulating signals. In particular, mesenchymal stem cells with their immunomodulatory properties can create a specific immune microenvironment to reduce inflammation, scarring, and support skin regeneration. This review presents an overview of current wound care techniques including skin tissue engineering and biomaterials as a novel and promising approach. We highlight the plasticity and different roles of immune cells, in particular macrophages during various stages of skin wound healing. These aspects are pivotal to promote the regeneration of nonhealing wounds such as ulcers in diabetic patients. We believe that a better understanding of the intrinsic immunomodulatory features of stem cells in implantable skin substitutes will lead to new translational opportunities. This, in turn, will improve skin tissue engineering and regenerative medicine applications.

2D and 3D electrospinning technologies for the fabrication of nanofibrous scaffolds for skin tissue engineering: A review

2020 ◽  
Vol 12 (4) ◽  
Author(s):  
Antonios Keirouz ◽  
Michael Chung ◽  
Jaehoon Kwon ◽  
Giuseppino Fortunato ◽  
Norbert Radacsi
Keyword(s):  
Tissue Engineering ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Nanofibrous Scaffolds ◽  
2D And 3D
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