scholarly journals The Effects of Methanolic Extract of Melissa officinalis on Experimental Gastric Ulcers in Rats

2016 ◽  
Vol 18 (7) ◽  
Author(s):  
Arezoo Saberi ◽  
Elham Abbasloo ◽  
Gholamreza Sepehri ◽  
Mahnaz Yazdanpanah ◽  
Ehsan Mirkamandari ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Gastric Ulcers ◽  
Melissa Officinalis

Cytoprotective and antisecretory properties of methanolic extract of Distemonanthus benthamianus (Caesalpiniaceae) stem bark on acute gastric ulcer in rats

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Vanessa Mba Matah Marthe ◽  
Gilbert Ateufack ◽  
Marius Mbiantcha ◽  
William Yousseu Nana ◽  
Albert Donatien Atsamo ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Gastric Lesion ◽  
Stem Bark ◽  
Male Rats ◽  
Gastric Ulcers ◽  
Gastric Lesions ◽  
Mucus Production ◽  
Antisecretory Activity ◽  
Antisecretory Effect ◽  
Pylorus Ligation

AbstractObjectivesIn African traditional medicine, Distemonanthus benthamianus (Caesalpiniaceae) is used to treat many diseases including gastric ulcers. We evaluated in this study, the cytoprotective and antisecretory properties of the methanolic extract of the stem bark of this plant using different technics of gastric lesion induction.MethodsCytoprotective and antisecretory activity of the methanolic extract of D. benthamianus stem bark was evolved through six methods of gastric lesion induction in experimental Wistar male rats (150–200 g): (1) gastric lesions induced by HCl/ethanol, (2) gastric lesions induced by Indomethacin- HCl/ethanol, (3) gastric lesion induced by Indomethacin, (4) gastric lesions induced by Pylorus ligation, (5) gastric lesions induced by histamine-Pylorus ligation, (6) gastric lesions induced by carbachol-Pylorus ligation. Mucus and gastric mucosal ulceration were evaluated. pH, gastric volume, and acidity were quantified in all pylorus ligation induction technics. Nitric oxide (NO) level was determined in indomethacin induced gastric ulcers.ResultsAt different doses (125, 250 and 500 mg/kg), extract reduced significantly the ulcer index. In all models used, that is 100.00% with HCl/ethanol; 100.00% with HCl/ethanol/indomethacin; 95.70% with Indomethacin; 74.79% with pylorus ligation, 95.94% histamine-Pylorus ligation, 99.54% carbachol-Pylorus ligation at the highest dose of 500 mg/kg. The lesion formation reduces in all the methods used followed by a significant increase of mucus production. The pylorus ligation technic revealed that the extract has an antisecretory activity.ConclusionsThe methanolic extract of D. benthamianus stem bark has both cytoprotective and antisecretory effects. This extract exerts its antisecretory effect trough cholinergic and histaminergic pathways.

scholarly journals In vivo effect of Piper sarmentosum methanolic extract on stress-induced gastric ulcers in rats

2019 ◽  
Vol 15 (1) ◽  
pp. 223-231 ◽  
Author(s):  
Mohd Fahami Nur Azlina ◽  
Hj Mohd Saad Qodriyah ◽  
Muhamad Nurul Akmal ◽  
Ibrahim Abdel Aziz Ibrahim ◽  
Yusof Kamisah
Keyword(s):  
Methanolic Extract ◽  
Gastric Ulcers

Influence of Melissa officinalis methanolic extract on hyperthyroidism in a rat model

2021 ◽  
Vol 0 (0) ◽  
pp. 0
Author(s):  
NeveinN Fadl ◽  
FathiaA Mannaa ◽  
KhaledG Abdel-Wahhab ◽  
LailaK Hassan ◽  
RehabF Taher ◽  
...  
Keyword(s):  
Rat Model ◽  
Methanolic Extract ◽  
Melissa Officinalis

scholarly journals Methanolic Extract of Distemonanthus benthamianus (Caesalpiniaceae) Stem Bark Suppresses Ethanol/Indomethacin-Induced Chronic Gastric Injury in Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Vanessa Mba Matah Marte ◽  
Gilbert Ateufack ◽  
Marius Mbiantcha ◽  
Albert Donatien Atsamo ◽  
Carine Flore Adjouzem ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Protein Denaturation ◽  
Hematological Parameters ◽  
Mechanisms Of Action ◽  
Male Rats ◽  
Gastric Ulcers ◽  
Gastric Injury ◽  
Ros Production ◽  
Tnf Α ◽  
Anti Inflammatory

Distemonanthus benthamianus (Caesalpiniaceae) is a plant from the Cameroon pharmacopoeia very widely used in the treatment of many pathologies among which are gastrointestinal disorders. The main purpose of this study was to assess the healing properties of gastric ulcer from the methanolic extract of Distemonanthus benthamianus and its mechanisms of action. The healing properties of gastric ulcers (chronic ulcer model induced by ethanol and indomethacin) were evaluated in vivo in adult male rats, while the mechanisms of action were evaluated in vitro by anti-inflammatory assay (protein denaturation, cyclooxygenase, and lipoxygenase assays) and immunomodulatory assay (ROS production (using technical chemiluminescence), cytokine (TNF-α, IL-1β, IL-6) production (using ELISA), proliferation of T cells (using liquid scintillation counter), and cytotoxicity (using MTT assay)). The methanolic extract of Distemonanthus benthamianus inhibited protein denaturation (75.63%) and the activities of cyclooxygenase (78.92%) and 5-lipoxygenase (81.54%). The extract also significantly ( p < 0.001 ) inhibited intracellular and extracellular ROS production and T cell proliferation and reduced significantly ( p < 0.01 , p < 0.001 ) TNF-α, IL-1β, IL-6, and PGE2 production. At all doses (125, 250, and 500 mg/kg), the extract significantly reduces the ulceration index and the area of ulceration and significantly increases the mass of gastric mucus. In addition, the extract significantly decreases the level of MDA, significantly increases the activities of catalase and glutathione, and then improves the hematological parameters in sick animals. Histological micrographs show that in the presence of the extract, there is advanced reepithelialization with recovery of the ulcerated epithelium. Thus, the extract of Distemonanthus benthamianus has healing properties against gastric ulcers which are associated with its anti-inflammatory, immunomodulatory, and antioxidant effects.

scholarly journals Antiulcerogenic activity of methanol extract from Ipomoea asarifolia leaves in Wistar rats

2021 ◽  
Vol 12 (2) ◽  
pp. 89-95
Author(s):  
A.E. Aiwonegbe ◽  
I.F. Omenai ◽  
K.O. Ogbeide ◽  
G.O. Benjamin ◽  
I.R. Eikpe
Keyword(s):  
Gastric Ulcer ◽  
Wistar Rats ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Protective Action ◽  
Gastric Ulcers ◽  
Protective Agent ◽  
Dependent Manner ◽  
Ipomoea Asarifolia ◽  
Antiulcerogenic Activity

The phytochemical constituents and the antiulcerogenic activity of methanolic extract from Ipomoea asarifolia leaf were investigated to ascertain its medicinal potentials. The study revealed that the leaf contained saponins, tannins, alkaloids, terpenoids and eugenols. The methanolic extract was used as a protective agent against gastric ulcer in Wistar rats, using the experimental model of gastric ulcer (lesion) induced by ethanol. The ulcer count for the methanol extract ranged from 0.00 to 2.00, while the ulcer index ranged between 0.33 to 2.00 for all concentrations (100, 200 and 400mg/kg). Percentage inhibition at 100, 200 and 400mg/kg were respectively 25.09%, 12.73% and 87.64%. The extract prevented acute gastric mucosal injury induced by absolute ethanol in a non dose-dependent manner. This is because the protective action was observed at the lowest and highest doses, but not an intermediate dose of the extract. The results obtained indicate that the leaf extract has antiulcerogenic importance and may be utilized for the treatment of gastric ulcers.

scholarly journals Prostaglandin Analogous and Antioxidant Activity Mediated Gastroprotective Action ofTabernaemontana divaricata(L.) R. Br. Flower Methanolic Extract against Chemically Induced Gastric Ulcers in Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-18 ◽  
Author(s):  
Mohammed Safwan Ali Khan ◽  
Abdul Manan Mat Jais ◽  
Adiba Afreen
Keyword(s):  
Superoxide Dismutase ◽  
Total Protein ◽  
Methanolic Extract ◽  
Histopathological Examination ◽  
Basic Mechanism ◽  
Gastric Ulcers ◽  
Antiulcer Activity ◽  
Ulcer Index ◽  
Mass Spectral ◽  
Antioxidant Mechanism

The present study was conducted to evaluate the antiulcerogenic effect and recognize the basic mechanism of action ofTabernaemontana divaricata(L.) R. Br. flowers.T. divaricataflower methanolic extract (TDFME) was screened for antiulcer activity versus aspirin and ethanol induced gastric ulcers at three doses—125, 250, and 500 mg/kg—orally using misoprostol as a standard. Besides histopathological examination, seven parameters, that is, ulcer index, total protein, nonprotein sulphhydryls, mucin, catalase, malondialdehyde, and superoxide dismutase levels, were estimated. In addition to HPLC profiling, GC-MS analysis and electrospray ionization—high resolution mass spectral (ESI-HRMS) analysis of crude TDFME were carried out in an attempt to identify known phytochemicals present in the extract on the basis ofm/zvalue. The results revealed a significant increase in the levels of catalase, superoxide dismutase, mucin, and nonprotein sulphhydryls, while they revealed a reduction in ulcer index, the levels of total protein, and malondialdehyde. Histopathological observations also demonstrated the protective effect. Though all the doses of TDFME exhibited gastroprotective function, higher doses were found to be more effective. Mass spectral analysis gave a few characteristicm/zvalues suggesting the presence of a few known indole alkaloids, while HPLC profiling highlighted the complexity of the extract. TDFME was found to exhibit its gastroprotective effect through antioxidant mechanism and by enhancing the production of gastric mucous.

scholarly journals Gastroprotective Mechanism and Ulcer Resolution Effect of Cyrtocarpa procera Methanolic Extract on Ethanol-Induced Gastric Injury

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Wendy Itzel Escobedo-Hinojosa ◽  
Erika Gomez-Chang ◽  
Karina García-Martínez ◽  
Raquel Guerrero Alquicira ◽  
Alexandre Cardoso-Taketa ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Clinical Symptoms ◽  
Gastric Ulcers ◽  
Gastric Injury ◽  
Inflammatory Infiltration ◽  
Area Reduction ◽  
Quality Of Ulcer Healing ◽  
Gastric Mucosal Erosions ◽  
Ulcer Treatment

Gastric ulcers are a worldwide health problem and their poor healing is one of the most important causes for their recurrence. We have previously reported the remarkable gastroprotective and anti-Helicobacter pylori activities of the methanolic extract (CpMet) of Cyrtocarpa procera bark. This work investigates, in a murine model, the CpMet gastroprotective mechanism and establishes its preclinical efficacy in the resolution of ethanol-induced gastric ulcers. The results showed that the gastroprotective activity of CpMet is mainly associated with endogenous NO and prostaglandins, followed by sulfhydryl groups and KATP channels. Furthermore, CpMet (300 mg/kg, twice a day) orally administered during 20 consecutive days promoted an ulcer area reduction of 62.65% at the 20th day of the treatment. The effect was confirmed macroscopically by the alleviation of gastric mucosal erosions and microscopically by an increase in mucin content and a reduction in the inflammatory infiltration at the site of the ulcer. No clinical symptoms or signs of toxicity were observed in the treated animals. The results indicate the safety and efficacy of CpMet in promoting high quality of ulcer healing by different mechanisms, but mostly through cytoprotective and anti-inflammatory effects, making it a promising phytodrug for ulcer treatment.

scholarly journals LC-MS Analysis and Antioxidant Activity of the Hydro-alcoholic Extract of Melissa Officinalis L. From Algeria

2020 ◽  
Vol 15 (2) ◽  
pp. 78-87
Author(s):  
Brahim Ben Aicha ◽  
Rachid Rouabhi ◽  
Salim Gasmi ◽  
Chawki Bensouici ◽  
Hichem Mohammedi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Mass Spectrometry Analysis ◽  
Alcoholic Extract ◽  
Scavenging Activity ◽  
Melissa Officinalis ◽  
Caftaric Acid ◽  
Pharmaceutical Industries

The present work focuses on evaluation of the chemical composition and antioxidant activity of the hydro-methanolic extract of Melissa officinalis from Algeria. The liquid chromatography-mass spectrometry analysis allowed the identification of six compounds: caffeic acid, caftaric acid, hydroxyjasmonic acid glucoside, caftaric acid glucoside, rosmarinic acid and sagerinic acid. The in-vitro antioxidant activity of the hydro-methanolic extract was evaluated by using four different methods including: radical scavenging assay (DPPH), scavenging activity (ABTS), cupric reducing antioxidant capacity, and ferric reducing power assay. The extract exhibited a relatively strong antioxidant activity compared to the synthetic antioxidants. The highest radical scavenging activity was registered using DPPH and ABTS methods, IC50= 20.53±2.64 μg/mL and 22.50±0.67 μg/mL, respectively. These results suggest that Melissa officinalis L. could be considered a potential source of natural antioxidants with potential interest in the agrochemical and pharmaceutical industries.

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