Isolation, Identification and Antimicrobial Susceptibility Pattern of Tatumella ptyseos Strains Isolated From Powdered Infant Formula Milk Consumed in Neonatal Intensive Care Unit: First Report From Iran

This study was the first conducted in Brazil to evaluate the presence of Enterobacter sakazakii in milk-based powdered infant formula manufactured for infants 0 to 6 months of age and to examine the conditions of formula preparation and service in three hospitals in São Paulo State, Brazil. Samples of dried and rehydrated infant formula, environments of milk kitchens, water, bottles and nipples, utensils, and hands of personnel were analyzed, and E. sakazakii and Enterobacteriaceae populations were determined. All samples of powdered infant formula purchased at retail contained E. sakazakii at <0.03 most probable number (MPN)/100 g. In hospital samples, E. sakazakii was found in one unopened formula can (0.3 MPN/100 g) and in the residue from one nursing bottle from hospital A. All other cans of formula from the same lot bought at a retail store contained E. sakazakii at <0.03 MPN/100 g. The pathogen also was found in one cleaning sponge from hospital B. Enterobacteriaceae populations ranged from 101 to 105 CFU/g in cleaning aids and <5 CFU/g in all formula types (dry or rehydrated), except for the sample that contained E. sakazakii, which also was contaminated with Enterobacteriaceae at 5 CFU/g. E. sakazakii isolates were not genetically related. In an experiment in which rehydrated formula was used as the growth medium, the temperature was that of the neonatal intensive care unit (25°C), and the incubation time was the average time that formula is left at room temperature while feeding the babies (up to 4 h), a 2-log increase in levels of E. sakazakii was found in the formula. Visual inspection of the facilities revealed that the hygienic conditions in the milk kitchens needed improvement. The length of time that formula is left at room temperature in the different hospitals while the babies in the neonatal intensive care unit are being fed (up to 4 h) may allow for the multiplication of E. sakazakii and thus may lead to an increased health risk for infants.

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Bacterial Isolates and its Antibiotic Susceptibility Pattern in NICU

Kathmandu University Medical Journal ◽

10.3126/kumj.v11i1.11030 ◽

2014 ◽

Vol 11 (1) ◽

pp. 66-70 ◽

Cited By ~ 13

Author(s):

S Shrestha ◽

NC Shrestha ◽

S Dongol Singh ◽

RPB Shrestha ◽

S Kayestha ◽

...

Keyword(s):

Intensive Care Unit ◽

Intensive Care ◽

Neonatal Intensive Care Unit ◽

Neonatal Intensive Care ◽

Bacterial Isolates ◽

Susceptibility Pattern ◽

Gram Positive ◽

Antibiotic Susceptibility Pattern ◽

Gram Negative ◽

Nosocomial Sepsis

Background Neonatal sepsis is one of the major causes of morbidity and mortality among the newborns in the developing world. Objectives To determine the common bacterial isolates causing sepsis in neonatal intensive care unit and its antibiotic susceptibility pattern. Methods A one year discriptive prospective study was conducted in neonatal intensive care unit to analyse the results of blood culture and to look into the sensitivity of the commonly used antibiotics. Results The blood culture yield by conventional method was 44.13% with nosocomial sepsis accounting for 10.79%. 84.08% were culture proven early onset sepsis and 15.95% were late onset sepsis. Klebsiella infection was the commonest organism isolated in early, late and nosocomial sepsis but statistically not significant. Gram positive organisms were 39.36% in which Staphylococcus aureus was the leading microorganism followed by coagulase negative staphylococcus areus. Gram negative organisms were 60.64% amongst them Klebsiella was the most often encountered followed by Pseudomonas. The most common organism Klebsiella was 87.5% and 78.3% resistance to ampicillin and gentamycin respectively. Among gram negative isolates 87.5% and 77.2% were resistance to ampicillin and gentamycin respectively. Among gram positive isolates 58.5% and 31.5% resistance were noted to ampicillin and gentamycin respectively. Resistance to cefotaxim to gram negative and gram positive isolates were 87.34% and 59.35% respectively. Conclusion Klebsiella is most common organism which is almost resistance to first line antibiotics. Resistance to both gram negative and gram positive isolates among firstline antibiotics and even with cefotaxim is emerging and is a major concern in neonatal intensive care unit. DOI: http://dx.doi.org/10.3126/kumj.v11i1.11030 Kathmandu University Medical Journal Vol.11(1) 2013: 66-70

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Biofilm Production in Clinical Isolates and Their Antimicrobial Susceptibility Pattern in the Intensive Care Unit of a Tertiary Care Hospital in Rajasthan

Journal of Mahatma Gandhi University of Medical Sciences and Technology ◽

10.5005/jp-journals-10057-0035 ◽

2017 ◽

Vol 2 (2) ◽

pp. 55-60

Author(s):

Neha Gupta ◽

Sweta Gupta ◽

Malvika Sharma ◽

Shanoo Sharma

Keyword(s):

Intensive Care Unit ◽

Intensive Care ◽

Antimicrobial Susceptibility ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Clinical Isolates ◽

Care Hospital ◽

Susceptibility Pattern ◽

Antimicrobial Susceptibility Pattern ◽

Biofilm Production

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Antimicrobial resistance of bacterial pathogens in a Neonatal Intensive Care Unit

Bangabandhu Sheikh Mujib Medical University Journal ◽

10.3329/bsmmuj.v11i1.35133 ◽

2018 ◽

Vol 11 (1) ◽

pp. 25 ◽

Cited By ~ 1

Author(s):

Farzana Ahmed ◽

Aftab Yousuf Raj ◽

Lutfun Nahar ◽

Zahidul Hasan

Keyword(s):

Intensive Care Unit ◽

Intensive Care ◽

Neonatal Intensive Care Unit ◽

Neonatal Intensive Care ◽

Treatment Options ◽

Gram Positive ◽

Gram Negative ◽

Antimicrobial Susceptibility Pattern ◽

Antibiotics Sensitivity ◽

Combination Preparation

The aim of this study was to identify the antimicrobial susceptibility pattern and relevant treatment options in a neonatal intensive care unit from January 2012 and June 2016. Out of the total 78 culture positive samples, Gram positive and Gram negative microorganisms were 26% and 74% respectively. Acinetobacter remained the predominant isolate (32.1%) followed by Klebsiella species (18.0%). Most of the Gram positive isolates exhibited higher resistance to penicillin, cephalosporin, macrolides, gentamycin and quinolones. Gram positive isolates had sensitivity of 100% to linezolid, vancomycin, chloramphenicol followed by rifampicin (84%). In comparison to other commonly used antibiotics, sensitivity to these four medicines was statistically significant (p<0.05). Similarly, most of the Gram negative bacteria showed resistance to cephalosporin, aminoglycosides. About two-third cases showed resistant to meropenum, quinolones and combination preparation of piperacillin and tazobactam. Overall sensitivity among the Gram negative isolates was to polymixin B (100%) and minocycline (97%), followed by colistin (83%). In comparison to other commonly used antibiotics, sensitivity to these three medicines was statistically significant (p<0.05).

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