scholarly journals Protection Against Radiation-induced Duox1 and Duox2 Upregulation in Rat’s Lung Tissues by a Combination of Curcumin and L-Selenomethionine

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Masoud Najafi ◽  
Peyman Amini ◽  
Hana Saffar ◽  
Sedighe Kolivand ◽  
Elahe Motevaseli ◽  
...  
Keyword(s):  
Experimental Study ◽  
Free Radicals ◽  
Lung Injury ◽  
Male Rats ◽  
Interleukin 4 ◽  
Injury Treatment ◽  
Radiation Induced ◽  
Lung Irradiation ◽  
Stimulation Of

Background: It has been proposed that increased levels of pro-inflammatory and pro-fibrotic cytokines play a key role in radiation-induced lung injury. Interleukin-4 (IL-4) and IL-13 are two pro-fibrotic cytokines that promote the production of free radicals through stimulation of Duox1 and Duox2. In this experimental study, we aimed to evaluate the expression of IL4Ra1, Duox1, IL13Ra2, and Duox2 genes following rat’s lung irradiation. Objectives: Also, we detected the modulatory effect of a combination of curcumin and L-selenomethionine on the expression of these genes. Methods: Twenty male rats were divided into four groups as G1: control (no treatment or radiation); G2: treatment with a combination of curcumin and L-selenomethionine; G3: radiation; G4: radiation plus a combination of curcumin and L-selenomethionine. sixty-seven days after irradiation, rats were killed for detecting the expression of IL4Ra1, IL13Ra2, Duox1, and Duox2. Results: The results showed no detectable expression for IL13Ra2, while the expression of IL4Ra1, Duox1, and Duox2 was increased. Treatment with a combination of curcumin and L-selenomethionine could attenuate the expression of these genes. Conclusions: This study proposes that upregulation of Duox1 and Duox2 may be involved in radiation-induced lung injury. Treatment with a combination of curcumin and L-selenomethionine may be useful for the mitigation of lung injury through modulation of these genes.

scholarly journals Protective Effect of Selenium-L-methionine on Radiation-induced Acute Pneumonitis and Lung Fibrosis in Rat

2019 ◽  
Vol 14 (2) ◽  
pp. 157-164 ◽  
Author(s):  
Peyman Amini ◽  
Sedighe Kolivand ◽  
Hana Saffar ◽  
Saeed Rezapoor ◽  
Elahe Motevaseli ◽  
...  
Keyword(s):  
Immune Responses ◽  
Morphological Properties ◽  
Interleukin 4 ◽  
Protective Effects ◽  
Receptor Subunit ◽  
Dual Oxidase ◽  
Radiation Induced ◽  
Lung Irradiation ◽  
60Co Gamma Rays ◽  
Interleukin 4 Receptor

Background: In this study, we aimed to detect the changes in the level of interleukin (IL)-4 and IL-13 cytokines and their downstream genes including interleukin-13 receptor subunit alpha-2 (IL13Ra2), interleukin-4 receptor subunit alpha-1 (IL4Ra1), dual oxidase 1 (DUOX1) and dual oxidase 2 (DUOX2). The protective effects of Selenium-L-methionine on radiation-induced histopathological damages and changes in the level of these cytokines and genes were detected. Methods: Four groups of 20 rats (5 rats in each) namely, control; Selenium-L-methionine, radiation and radiation plus Selenium-L-methionine were used in this study. 4 mg/kg of Selenium-Lmethionine was administered 1 day before irradiation and five consecutive days after irradiation. Irradiation was done using a dose of 15 Gy 60Co gamma rays at 109 cGy/min. All rats were sacrificed 10 weeks after irradiation for detecting changes in IL-4 and IL-13 cytokines, the expressions of IL13Ra2, IL4Ra1, Duox1 and Duox2 and histopathological changes. Results: The level of IL-4 but not IL-13 increased after irradiation. This was associated with increased expression of IL4Ra1, Duox1 and Duox2, in addition to changes in morphological properties. Selenium-L-methionine could attenuate all injury markers following lung irradiation. Conclusion: Selenium-L-methionine can protect lung tissues against toxic effects of ionizing radiation. It is possible that the modulation of immune responses and redox interactions are involved in the radioprotective effect of this agent.

scholarly journals Crossed Pathways for Radiation-Induced and Immunotherapy-Related Lung Injury

2021 ◽  
Vol 12 ◽  
Author(s):  
Zengfu Zhang ◽  
Jialin Zhou ◽  
Vivek Verma ◽  
Xu Liu ◽  
Meng Wu ◽  
...  
Keyword(s):  
Lung Injury ◽  
Signaling Pathways ◽  
Radiation Pneumonitis ◽  
Checkpoint Inhibitors ◽  
Therapeutic Targets ◽  
Normal Lung ◽  
Injury Treatment ◽  
Radiation Induced ◽  
The Common ◽  
Underlying Mechanisms

Radiation-induced lung injury (RILI) is a form of radiation damage to normal lung tissue caused by radiotherapy (RT) for thoracic cancers, which is most commonly comprised of radiation pneumonitis (RP) and radiation pulmonary fibrosis (RPF). Moreover, with the widespread utilization of immunotherapies such as immune checkpoint inhibitors as first- and second-line treatments for various cancers, the incidence of immunotherapy-related lung injury (IRLI), a severe immune-related adverse event (irAE), has rapidly increased. To date, we know relatively little about the underlying mechanisms and signaling pathways of these complications. A better understanding of the signaling pathways may facilitate the prevention of lung injury and exploration of potential therapeutic targets. Therefore, this review provides an overview of the signaling pathways of RILI and IRLI and focuses on their crosstalk in diverse signaling pathways as well as on possible mechanisms of adverse events resulting from combined radiotherapy and immunotherapy. Furthermore, this review proposes potential therapeutic targets and avenues of further research based on signaling pathways. Many new studies on pyroptosis have renewed appreciation for the value and importance of pyroptosis in lung injury. Therefore, the authors posit that pyroptosis may be the common downstream pathway of RILI and IRLI; discussion is also conducted regarding further perspectives on pyroptosis as a crucial signaling pathway in lung injury treatment.

DER EINFLUSS VON RESERPIN AUF DIE ANTITHYREOIDALE WIRKUNG VON KALIUMPERCHLORAT

1962 ◽  
Vol 39 (3) ◽  
pp. 423-430
Author(s):  
H. L. Krüskemper ◽  
F. J. Kessler ◽  
E. Steinkrüger
Keyword(s):  
Thyroid Gland ◽  
Male Rats ◽  
Normal Male ◽  
Tissue Respiration ◽  
List Type ◽  
Morphological Alterations ◽  
Hormone Synthesis ◽  
Stimulation Of

ABSTRACT 1. Reserpine does not inhibit the tissue respiration of liver in normal male rats (in vitro). 2. The decrease of tissue respiration of the liver with simultaneous morphological stimulation of the thyroid gland after long administration of reserpine is due to a minute inhibition of the hormone synthesis in the thyroid gland. 3. The morphological alterations of the thyroid in experimental hypothyroidism due to perchlorate can not be prevented with reserpine.

Macrophage Activation in Stellate Ganglia Contributes to Lung Injury‐Induced Arrhythmogenesis in Male Rats

2021 ◽  
Author(s):  
Juan Hong ◽  
Ryan J. Adam ◽  
Lie Gao ◽  
Taija Hahka ◽  
Zhiqiu Xia ◽  
...  
Keyword(s):  
Lung Injury ◽  
Macrophage Activation ◽  
Male Rats ◽  
Stellate Ganglia
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