Hematological and Morphological Changes in the Peripheral Blood Smear of Patients with COVID-19

Background: The novel coronavirus is responsible for a widespread pandemic, causing multiple clinical manifestations. Peripheral blood films have shown changes in white blood cell (WBC) morphology and counts, as well as platelet abnormalities. Atypical and reactive lymphocytes may also be observed in the blood film, which suggests mild leukoerythroblastosis. Objectives: The present study aimed to evaluate the hematological and morphological changes in the peripheral blood smear of patients with COVID-19. Methods: This study was conducted on 175 patients diagnosed with COVID‐19 infection. Blood samples were collected from the patients and examined for the estimation of blood parameters, differential cell count, and presence of atypical lymphocytes. Results: The mean age of the patients was 53.73 years, and males constituted 52.6% of the patients. The majority had normal hemoglobin levels. WBC count was normal in 60.6% of the patients, while 74.3% had normal platelet count, and thrombocytopenia was detected in 23.4%. In addition, differential WBC count indicated that 56.6% of the patients had normal neutrophils, while lymphopenia was detected in 28 and 77.7% had a low eosinophil count. The peripheral blood smears showed atypical lymphocytes with deeply basophilic cytoplasm in 21.14% of the patients. Conclusions: Blood film examination may show multiple abnormal findings in patients with COVID-19 infection. Neutrophilia and lymphopenia with the presence of atypical lymphocytes are frequently observed as well. Low eosinophil count is also highly common. Although platelets showed changes, they were normal or reduced in most cases.

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Congenital Malaria: A Rare Cause of Splenomegaly and Anemia in an American Infant

PEDIATRICS ◽

10.1542/peds.60.2.209 ◽

1977 ◽

Vol 60 (2) ◽

pp. 209-212

Author(s):

D. Thompson ◽

C. Pegelow ◽

A. Underman ◽

D. Powars

Keyword(s):

Plasmodium Vivax ◽

Hemolytic Anemia ◽

Peripheral Blood ◽

Endemic Area ◽

Blood Smear ◽

Clinical Manifestations ◽

Peripheral Blood Smear ◽

Pregnancy Malaria ◽

Congenital Malaria

A 38-day-old infant had fever, jaundice, hepatosplenomegaly, and a hemolytic anemia. A peripheral blood smear demonstrated intraerythrocytic malarial parasites identified as Plasmodium vivax. Maternal and infant sera contained antibodies to this species. A directed history revealed the mother had suffered several febrile illnesses in Mexico during her pregnancy. Malaria had not been diagnosed nor was it considered at the time of her delivery at this hospital. Review of this and six other cases of congenital malaria reported in this country since 1950 indicates clinical manifestations seldom appear before 3 weeks of age. Although these signs are more frequently associated with other transplacental infections, their occurrence in an infant whose mother is from or who has traveled in an endemic area should prompt consideration of the diagnosis of congenital malaria.

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Morphological Changes of Red Blood Cells in Peripheral Blood Smear of Patients with Pregnancy-related Hypertensive Disorders

Archives of Medical Research ◽

10.1016/j.arcmed.2015.07.003 ◽

2015 ◽

Vol 46 (6) ◽

pp. 479-483 ◽

Cited By ~ 6

Author(s):

Jorge Daniel Hernández Hernández ◽

Onésimo Rangel Villaseñor ◽

Javier Del Rio Alvarado ◽

Roberto Ortega Lucach ◽

Arturo Zárate ◽

...

Keyword(s):

Red Blood Cells ◽

Peripheral Blood ◽

Blood Cells ◽

Blood Smear ◽

Morphological Changes ◽

Peripheral Blood Smear ◽

Hypertensive Disorders

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Prevalence of latex allergy among clinical laboratory workers of kerala: diagnostic approach by questionnaire survey (QBLH), hematological study and ige screening with ELISA

Asian Journal of Health Sciences ◽

10.15419/ajhs.v1i1.413 ◽

2013 ◽

Vol 1 (1) ◽

pp. 38-41

Author(s):

T M Sagi ◽

Jose Sebastian ◽

R Harikumaran Nair

Keyword(s):

Peripheral Blood ◽

Questionnaire Survey ◽

Eosinophil Count ◽

Blood Smear ◽

Clinical Laboratory ◽

Peripheral Blood Smear ◽

Latex Allergy ◽

Antibody Assay ◽

Latex Glove ◽

Laboratory Workers

The use of latex glove has increased several folds due to concern about blood borne infections.Latex allergy has become a global epidemic affecting health care workers, industry workers and patients.The objective of the study was to determine the prevalence and risk factors of latex glove allergy among clinical laboratory workers in Kerala, India. The study also aimed to find out easy diagnostic procedures for the assessment of latex glove allergy. Methodology: Epidemiological study by a modified questionnaire based latex hypersensitivity, Haematological studies like Peripheral Blood Smear examination for eosinophilia, Absolute Eosinophil Count and IgE specific antibody assay. Observations: Out of 234 laboratory workers participated in the Questionnaire Based Latex Hypersensitivity study, all responded well to the study, seven shows positivity. The positive seven subjects were included in further studies. The blood samples of them revealed high eosinophilia in peripheral blood smear with high absolute eosinophilic count .IgE antibody shows high value in the five cases. Conclusion: Due to lower exposure to sensitizing latex allergens, there is low prevalence of latex allergy in clinical laboratory workers of Kerala. Questionnaire survey, Peripheral Blood Smear, Absolute Eosinophil Count and IgE antibody assay can be used as a useful tool for diagnosis of latex allergy than using skin prick test with more risk.

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PENENTUAN KRITERIA PENILAIAN KESAN JUMLAH LEUKOSIT PADA PEMERIKSAAN APUSAN DARAH TEPI

Jurnal Kesehatan Panrita Husada ◽

10.37362/jkph.v3i2.156 ◽

2018 ◽

Vol 3 (2) ◽

pp. 27-34

Author(s):

Aan Yulianingsih Anwar ◽

Nurhamsiah

Keyword(s):

Platelet Count ◽

Human Body ◽

Peripheral Blood ◽

Blood Smear ◽

Peripheral Blood Smear ◽

Blood Components ◽

Laboratory Observation ◽

Normal Platelet ◽

Zone Ii ◽

The Difference

Platelets are one of the blood components present in the human body, which play an important role in hemostasis. A good macroscopic and microscopic peripheral blood smear is essential in assessing success in the manufacture of peripheral blood smear preparations. This research was conducted on March, 9thto May, 31st2018 at Hematology Laboratory of Health Polytechnic of Makassar majoring in Health Analyst. The purpose of this study to determine the number of thrombocytes view ability and declared the impression of sufficient quantities. The type of research used in this study is laboratory observation that aims to determine the assessment criteria impression of the number of platelets in the peripheral blood smear. The results showed that the number of platelets in zone I obtained results the impression of the number of platelets 5-9 stated the amount of normal platelet impression, zone II obtained the result total number of platelets impression 6-10 stated the amount of normal platelet impression, and the impression of the results obtained III zone number of platelets 7-11 stated the number of the impression of normal platelets. Therefore, advisable to the laboratory to calculate the platelet count using the peripheral blood smear method by counting at zone II and in subsequent studies it is better to examine the difference in platelet counts in each zone in the peripheral blood smear.

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The Association Between Cellular Morphological Changes in Peripheral Blood Smear and Complications in Pediatric Burn Cases

Turkish Journal of Trauma and Emergency Surgery ◽

10.5505/tjtes.2011.13911 ◽

2011 ◽

Vol 17 (2) ◽

pp. 128-132

Author(s):

Mehmet Bozkurt ◽

Samet Vasfi Kuvat ◽

Emin Kapi ◽

Percin Karakol ◽

Abdulkadir Ozel ◽

...

Keyword(s):

Peripheral Blood ◽

Blood Smear ◽

Morphological Changes ◽

Peripheral Blood Smear

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And the Oscar goes to peripheral blood film for detection of lead poisoning in a complicated patient: A case report with review of laboratory clues

10.21203/rs.3.rs-95086/v1 ◽

2020 ◽

Author(s):

Amir Zamani ◽

Ehsan Sarraf Kazerooni ◽

S Saeed Kasaee ◽

Mohammad Hossein Anbardar ◽

Sahand Mohammadzadeh ◽

...

Keyword(s):

Bone Marrow ◽

Lead Poisoning ◽

Peripheral Blood ◽

Blood Smear ◽

Lead Level ◽

Peripheral Blood Smear ◽

Blood Film ◽

Short Period ◽

Bone Marrow Study ◽

Blood Smear Review

Abstract Background Peripheral blood smear examination is an invaluable laboratory test which provides the complete hematologic and/or non-hematologic picture of a case. In addition to verifying the results of automated cell counters, it has the potential to identify some pathologic morphologic changes that remain hidden using the cell counters alone. Case presentation A 40-year-old man with a 3 year history of alcohol intake and marijuana abuse presented with severe lower extremities bone pain and abdominal pain. Physical examination showed high blood pressure, high pulse rate and abdominal tenderness. He underwent extensive laboratory and imaging tests, and cholecystectomy and bone marrow study, without any definite diagnosis or improvement. Right after all these invasive, expensive and time consuming investigations during a month, finding a point on the peripheral blood smear by an expert led to the final diagnosis. The finding was coarse basophilic stippling in the red blood cells. Elevated blood lead level and the presence of ring sideroblast in the bone marrow study confirmed the diagnosis of lead poisoning and the patient responded well to chelator therapy in a short period. Conclusion This case clearly shows the value of peripheral blood smear review and its impact on the patient care. In order not to lose the cases, laboratories are recommended to design their own policy for peripheral blood smear review. Peripheral blood smear is the fastest, simplest, and most available screening test which could prevent many misdiagnoses and malpractices. It provides rich morphological information, among which basophilic stippling is highly suggestive of lead poisoning.

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Microfilaria: not always associated with eosinophilia

Tropical Doctor ◽

10.1177/0049475519833264 ◽

2019 ◽

Vol 49 (3) ◽

pp. 210-212

Author(s):

Neha Garg ◽

Shilpi More ◽

Monika Sharma ◽

Mrinalini Kotru

Keyword(s):

Low Power ◽

Peripheral Blood ◽

Eosinophil Count ◽

Blood Smear ◽

Parasitic Infection ◽

Peripheral Blood Smear ◽

Buffy Coat ◽

Blood Eosinophilia ◽

Wet Mount ◽

Peripheral Blood Eosinophilia

Filariasis is a parasitic infection seen predominantly in tropical and subtropical countries including India. In clinically suspected cases, examining a thick wet mount smear or a buffy coat film is most informative. In unsuspected cases, however, eosinophilia in a peripheral blood smear (PBS) may be the sole indicator of parasitaemia. A few cases of tissue microfilaria with the absence of peripheral blood eosinophilia (PBE) have been reported. Here, we report two cases of microfilaria in PBS in the absence of PBE. A routine screening of the tail end of all PBS at low power magnification is also advised as it may facilitate the detection of asymptomatic cases when there is a normal eosinophil count.

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Can the leukocyte`s parameters in peripheral blood smear predict risk of in-hospital death of patients undergoing high-risk gastrointestinal surgery?

Polish Journal of Surgery ◽

10.5604/01.3001.0012.7792 ◽

2018 ◽

Vol 91 (1) ◽

pp. 1-5

Author(s):

Michał Pluta ◽

Łukasz Krzych

Keyword(s):

High Risk ◽

Peripheral Blood ◽

Blood Smear ◽

Gastrointestinal Surgery ◽

Peripheral Blood Smear ◽

Full Blood Count ◽

Hospital Death ◽

Significant Difference ◽

Precise Analysis ◽

Wbc Count

Introduction. White blood cell (WBC) count constitutes a part of routine peripheral blood examination (FBC, full blood count). Precise analysis of leukocytes’ parameters in blood smear is usually performed only when leukopenia or leukocytosis is found. We aimed to assess the usefulness of leukocytes’ smear test in predicting in-hospital death of patients undergoing high-risk gastrointestinal (GI) surgery. Materials & Methods. We prospectively enrolled 101 subjects undergoing high-risk GI surgery from 01.01.2017 till 31.12.2017. Blood tests were performed preoperatively, with analysis focused on the assessment of total WBC count, as well as the count of neutrophils (NEUT), lymphocytes (LYM), monocytes (MONO), eosinophils (EOS) and basophils (BASO). Indices of NEUT/LYM (NLR) and MONO/LYM (MLR) were calculated. In-hospital mortality was considered the outcome. Results. Mortality reached 5%. There was no significant difference in WBC count between survivors and the deceased (p=0.2) and WBC failed to predict the outcome (AUC=0.69; p=0.3). MONO (p=0.009) and BASO (p=0.02) counts, as well as MLR (p=0.007) were significantly higher in patients who died. MONO count and MLR index predicted in-hospital death with good accuracy, respectively: AUC[MONO]=0.85 (p<0.001) and AUC[MLR]=0.86 (p<0.001). Other investigated parameters played no significant role in outcome prediction. Conclusion. Routine peripheral blood smear evaluation should be considered in all patients undergoing high-risk GI surgery, because the number of monocytes can be a valuable predictor of in-hospital death.

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A case with full clinical manifestations of Dorfman-Chanarin syndrome

Open Medicine ◽

10.2478/s11536-007-0010-x ◽

2007 ◽

Vol 2 (1) ◽

pp. 116-121

Author(s):

Fatma Çuhaci ◽

Rüya Mutluay ◽

Burhan Aksakal ◽

Yasemin Erten

Keyword(s):

Peripheral Blood ◽

Blood Smear ◽

Clinical Manifestations ◽

Autosomal Recessive Disorder ◽

Peripheral Blood Smear ◽

Congenital Ichthyosis ◽

Blood Smear Examination ◽

History Of ◽

Lipid Vacuoles ◽

Peripheral Leukocytes

AbstractDorfman-Chanarin syndrome (DCS), is a rare, autosomal recessive disorder associated with lipid metabolism. It is characterized by ichthyosiform nonbullous erythroderma, lipid vacuoles in peripheral leukocytes and variable involvement of organs. We report a Turkish man with the complete syndrome, who described family history of ichthyosis. To best of our knowledge this is the sixth case from Turkish origin to date. In addition to congenital ichthyosis he had also strabismus, horizantal nystagmus, bilateral neurosensory hearing loss, hepatomegaly and splenomegaly. Liver biopsy revealed hidrophic degeneration in hepatocytes, steatosis, enlargement and inflammation in portal areas and portal central fibrosis, consistent with cirrhosis. Write stained peripheral blood smear examination revealed lipid vacuoles in all of the neutrophils consistent with Jordan’s anomaly. We think that, it is essential to evaluate the peripheral blood smear of the patients with ichthyosis and also patients with DCS should be informed and warned about the results of consanquinous marriage.

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STUDI GAMBARAN HASIL PEMERIKSAAN APUSAN DARAH TEPI PADA IBU HAMIL DI LABORATORIUM RSUD H.A.SULTHAN DAENG RADJA KABUPATEN BULUKUMBA

Jurnal Kesehatan Panrita Husada ◽

10.37362/jkph.v3i2.155 ◽

2018 ◽

Vol 3 (2) ◽

pp. 13-26

Author(s):

Rahmat Aryandi ◽

Subakhir Salnus

Keyword(s):

Platelet Count ◽

Pregnant Women ◽

Blood Smear ◽

Leukocyte Count ◽

Marked Change ◽

Peripheral Blood Smear ◽

Morphological Examination ◽

Laboratory Observation ◽

Hematologic Disorder ◽

Normal Platelet

During pregnancy, there will be a marked change in anatomy, physiology and biochemistry since the onset of pregnancy and often lackof nutrient intake. Hematologic disorder is often found in pregnant women because it causes pregnant women more susceptible to disturbances in blood circulation, The purpose of this study to determine the description of blood smear results in pregnant women in the laboratory RSUD H.A.Sulthan Daeng Radja District. This research is descriptive with laboratory observation approach. The sample used in this study were 30 samples of pregnant women who checked themselves in the Laboratory of RSUDH.A.Sulthan Daeng Radka Bulukumba District. The result of this research showed the result of peripheral blood smear on the morphology of erythrocytes using 30 samples of pregnant women showed 14 samples (46,66%) normocytic normochrom and the remaining 16 samples were morphological variation (53,33%), on morphological examination and platelet count with using 30 samples of pregnant women showed each 29 samples had morphology and normal platelet counts with respectively 96.66% percentage and platelet aggregation and decreased platelet count (thrombocytopenia) with each persentase 3.33%. At leukocyte morphology examination using 30 samples of pregnant women showed 29 samples had normal morphology with 96,66% percentage and one sample with hypersegmentation with percentage 3,33%. normal leukocyte count at 9 samples with percentage 30% and leukocyte count increased at 21 samples with percentage 70%.

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