scholarly journals FTA Cards for Preservation of Nucleic Acids for Molecular Assays: A Review on the Use of Cytologic/Tissue Samples

2018 ◽  
Vol 142 (3) ◽  
pp. 308-312 ◽  
Author(s):  
Gilda da Cunha Santos
Keyword(s):  
Nucleic Acids ◽  
Molecular Techniques ◽  
Long Distance ◽  
High Quality ◽  
Molecular Assays ◽  
Fta Cards ◽  
Long Term Storage ◽  
Term Storage

Context.— Traditional methods for storing histologic and cytologic specimens for future use in molecular assays have consisted of either snap-freezing with cryopreservation or formalin-fixing, paraffin-embedding the samples. Although snap-freezing with cryopreservation is recommended for better preservation of nucleic acids, the infrastructure and space required for archiving impose challenges for high-volume pathology laboratories. Cost-effective, long-term storage at room temperature; relatively easy shipment; and standardized handling can be achieved with formalin-fixed, paraffin-embedded samples, but formalin fixation induces fragmentation and chemical modification of nucleic acids. Advances in next-generation sequencing platforms, coupled with an increase in diagnostic, prognostic, and predictive molecular biomarkers have created a demand for high-quality nucleic acids. To address issues of the quality of nucleic acid and logistics in sample acquisition, alternatives for specimen preservation and long-term storage have been described and include novel universal tissue fixatives, stabilizers, and technologies. Objective.— To collect, retrieve, and review information from studies describing the use of nucleic acids recovered from cytologic/tissue specimens stored on Flinders Technology Associates (FTA, GE Whatman, Maidstone, Kent, United Kingdom) cards for downstream molecular applications. Data Sources.— An electronic literature search in the PubMed (National Center for Biotechnology Information, Bethesda, Maryland) database allowed the selection of manuscripts addressing the use of FTA cards for storage of cytologic samples for molecular analysis. Only articles published in English were retrieved. Conclusions.— The use of FTA cards is a versatile method for fostering multicenter, international collaborations and clinical trials that require centralized testing, long-distance shipment, and high-quality nucleic acids for molecular techniques. Studies with controlled temperature are required to test the quality of recovered RNA after long-term storage.

scholarly journals Unconventional effects of long-term storage of microwave-modified chicken egg white lysozyme preparations

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Grzegorz Leśnierowski ◽  
Tianyu Yang ◽  
Renata Cegielska-Radziejewska
Keyword(s):  
Microwave Radiation ◽  
Egg White ◽  
Process Time ◽  
High Quality ◽  
Chicken Egg ◽  
Egg White Lysozyme ◽  
Physicochemical Changes ◽  
Long Term Storage ◽  
Term Storage

AbstractThermal modification is an effective method that induces significant expansion of the antimicrobial properties and other valuable properties of chicken egg white lysozyme. In our latest research, a new innovative method of enzyme modification was developed, in which microwave radiation was used as an energy source to process liquid lysozyme concentrate (LLC). After modification, high-quality preparations were obtained. However, long-term storage in a concentrated form initiated various processes that caused darkening over time and could also lead to other significant changes to their structure and, consequently, to their functional properties. This necessitated multidirectional research to explain this phenomenon. This paper presents the results of research aimed at assessing the physicochemical changes in the properties of microwave-modified lysozyme in the form of a liquid concentrate after long-term storage under refrigeration conditions. The assessment also considered the conditions under the acidity of the modifying medium and the duration of the microwave modification. The analysis showed that the values of the basic parameters determining the quality and usefulness of the modified enzyme significantly improved during long-term storage of the preparations. The greatest changes were observed in the preparations modified for the longest time and in the most acidic environment (process time 260 s, pH 2.0), the number of oligomers under these conditions increased by 18% after 12 months of holding, and the surface hydrophobicity increased by as much as 31%. In addition, microbiological tests showed that the preparations of microwave-modified lysozyme had an effect on gram-positive bacteria as well as on gram-negative, and this effect was significantly enhanced after 12 months. The results confirm that LLC modification with microwave radiation is a highly efficient method to prepare high-quality and high utility potential lysozyme. Notably, an interesting and important phenomenon was the observation of the unconventional behaviour of the preparations during their long-term storage, which increased their utility potential significantly.

Summer Pruning: An Ecological Alternative to Postharvest Calcium Treatment to Improve Storability of High Quality Apple cv. ‘Reinette du Canada’

2010 ◽  
Vol 16 (4) ◽  
pp. 343-350 ◽  
Author(s):  
M. Guerra ◽  
P.A. Casquero
Keyword(s):  
Titratable Acidity ◽  
Soluble Solids ◽  
High Quality ◽  
Long Term Storage ◽  
Postharvest Application ◽  
Bitter Pit ◽  
Best Parameter ◽  
Term Storage ◽  
Organic Orchards

Two strategies, summer pruning and postharvest Ca treatment, were studied in apple (Malus domestica Borkh) cv. ‘Reinette du Canada’ in order to analyze its effect on the fruit quality during storage. Summer pruning and Ca treatment reduced external and internal bitter-pits; so after 180 days of storage, both treatments decreased external bitter-pit by 10.0% and 16.7%, respectively. Summer pruning influenced color, firmness, total soluble solids and titratable acidity (TA) of fruit during storage, whereas Ca treatment only affected firmness and TA. Fruit from pruned trees had significant lower K and Mg than those from unpruned trees and Ca treatment increased Ca content. Orchard management, by means of summer pruning, combined with Ca postharvest application would be useful to prevent losses due to bitter-pit during storage in commercial orchards. However, in organic orchards, summer pruning would be the ecological alternative to decrease bitter-pit incidence during storage in high quality apple cv. ‘Reinette du Canada’. K/Ca ratio, on the peel at harvest, turned out to be the best parameter to correlate with external and internal bitter-pits during storage; so this ratio would be useful to predict bitter-pit on long-term storage.

scholarly journals The effect of aerosol treatment with calcium-based preparation on quality of table grape cultivar during storage

2021 ◽  
Vol 232 ◽  
pp. 03023
Author(s):  
Sofia Cherviak ◽  
Svetlana Levchenko ◽  
Vladimir Boyko ◽  
Dmitriy Belash
Keyword(s):  
Weight Loss ◽  
Table Grape ◽  
Oxidative Enzymes ◽  
Organoleptic Properties ◽  
Table Grapes ◽  
Long Term Storage ◽  
Organoleptic Characteristics ◽  
Term Storage

The main problems of grape storage are berry dehydration and rot. One of the solutions to reduce rot and preserve the quality of grapes during storage is the use of aerosol treatment with calcium content before storing. The aim of the study was to evaluate the effect of physiologically active substances based on calcium in various concentrations on the quality and organoleptic characteristics of table grape cvs. ‘Italia’ during long-term storage. The effectiveness of the studied systems of treatment was evaluated according to the content of sugars and titratable acids, the activity of the redox enzymes of polyphenol oxidase and peroxidase, weight loss of bunch and organoleptic properties. The greatest effectiveness established by using preparation of Master Green Ca, Brentax KCa, and Brentax Ca (in concentration 150g / 20l). Experimental treatments significantly decreased the weight loss of bunch (less than 4%), and preserved organoleptic properties of berry at a high level (8.2-8.9 points). Decrease in the activity of oxidative enzymes had a positive effect on the quality of table grapes and contributed to its better preservation. The data obtained make it possible to rationalize the system of long-term storage of grapes by using of aerosol treatment with calcium-based preparation.

scholarly journals The Technological Investigations Of Manufacturing Of Energy Chips

2015 ◽  
Vol 1 ◽  
pp. 220
Author(s):  
Ziedonis Miklašēvičs
Keyword(s):  
Moisture Content ◽  
Dry Mass ◽  
Quality Parameters ◽  
Heating Value ◽  
Long Term Storage ◽  
Lower Heating Value ◽  
Term Storage ◽  
Lower Heating

The methodology in Latvia forest industry provide to determine the quality of energy chips only in long- term storage places before selling. Due to the lack of hard empirical data about the quality parameters of energy chips in different phases of manufacturing process, this research paper consists of: - the identification and analyses of the factors that influenced the values of energy chips quality features such as: bulk density, moisture content, ash content, higher and lower heating value according to actual moisture content and per dry mass of the chips; - the methodology for determination the quality parameters of energy chips by analysis the wood moisture content and by choice the method of the manufacturing of energy chips.

scholarly journals EFFECT OF FREEZING ON THE MILK QUALITY OF COWS

2020 ◽  
pp. 54-57
Author(s):  
Lyubov Valentinovna Efimova ◽  
Tatyana Vyacheslavovna Zaznobina ◽  
Elena Vladimirovna Gatilova ◽  
Olga Valeryevna Ivanova
Keyword(s):  
Quality Indicators ◽  
Age Groups ◽  
Milk Quality ◽  
Long Term Storage ◽  
Before And After ◽  
Frozen State ◽  
Term Storage ◽  
Two Age Groups

Due to the fact that milk has a short shelf life, the issue of its freezing and long-term storage in the frozen state is relevant. A study was conducted on the effect of freezing on the milk quality after its defrosting of cows-mothers and cows-daughters of Red-Motley breed. As a result, it was established how milk quality indicators to change after five months of storage in a frozen state in cows of two age groups. A high correlation was established between quality indicators before and after freezing. The influence of the “freezing” factor on milk quality indicators was recognized statistically significant, the “generation” factor – not significant.

scholarly journals Modern technologies for utilizing tobacco wastes

2018 ◽  
Vol 80 (3) ◽  
pp. 259-264
Author(s):  
A. G. Mirgorodskaya ◽  
M. V. Shkidyuk ◽  
N. N. Matyukhina ◽  
T. A. Don
Keyword(s):  
Fractional Composition ◽  
Special Treatment ◽  
Long Term Storage ◽  
Tobacco Production ◽  
Taste Evaluation ◽  
Cut Tobacco ◽  
Term Storage ◽  
Modern Technologies

The problem of utilization or recycling industrial tobacco wastes is an important problem for tobacco industry. Economically preferable way for utilizing industrial tobacco wastes (tobacco scrap) is production smokeless products and hookah blends as wastes are utilized without special treatment. Monitoring of humidity and fractional composition of cut tobacco taken from tobacco rod/cigarette spoilage (tobacco scrap) is carried. Its long-term storage leads to increasing scrap and dust quantity. Researches on utilizing tobacco scrap for sniff and hookah blend production, which are considered to be less harmful compared to cigarettes, were carried. Chemical composition of American type tobacco and scrap was defined. Nicotine content varies from 0.9 to 2.4% and carbohydrates content – from 5.6 to 9.6%. Experimental samples of hookah blend were produced and their organoleptic and taste evaluation were carried. It was found that utilization of tobacco scrap is economically efficient for hookah blend production, as quality of final product made of leaf tobacco and tobacco scrap is similar. Experimental samples of sniff tobacco were produced in order to discover possibility of utilization industrial tobacco scrap for manufacturing this product. Consumer’s properties of sniff tobacco were evaluated by 100 points tasting protocol. This technology for sniff tobacco production from tobacco scrap allows manufacturing product of good quality, decreasing technological operations, rejecting stages of tobacco selection and its cutting and as the result decreasing cost of final product. The results of the research on utilizing tobacco scrap for hookah blend and sniff tobacco production proves optimality of recycling the tobacco wastes.

scholarly journals Rapid, Reproducible, Quantifiable NMR Metabolomics: Methanol and Methanol: Chloroform Precipitation for Removal of Macromolecules in Serum and Whole Blood

Metabolites ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 93 ◽  
Author(s):  
Cora McHugh ◽  
Thomas Flott ◽  
Casey Schooff ◽  
Zyad Smiley ◽  
Michael Puskarich ◽  
...  
Keyword(s):  
Human Serum ◽  
1H Nmr ◽  
Whole Blood ◽  
Nmr Spectra ◽  
High Quality ◽  
Healthy Human ◽  
Long Term Storage ◽  
The Impact ◽  
Term Storage

Background: Though blood is an excellent biofluid for metabolomics, proteins and lipids present in blood can interfere with 1d-1H NMR spectra and disrupt quantification of metabolites. Here, we present effective macromolecule removal strategies for serum and whole blood (WB) samples. Methods: A variety of macromolecule removal strategies were compared in both WB and serum, along with tests of ultrafiltration alone and in combination with precipitation methods. Results: In healthy human serum, methanol:chloroform:water extraction with ultrafiltration was compared to methanol precipitation with and without ultrafiltration. Methods were tested in healthy pooled human serum, and in serum from patients with sepsis. Effects of long-term storage at −80 °C were tested to explore the impact of macromolecule removal strategy on serum from different conditions. In WB a variety of extraction strategies were tested in two types of WB (from pigs and baboons) to examine the impact of macromolecule removal strategies on different samples. Conclusions: In healthy human serum methanol precipitation of serum with ultrafiltration was superior, but was similar in recovery and variance to methanol:chloroform:water extraction with ultrafiltration in pooled serum from patients with sepsis. In WB, high quality, quantifiable spectra were obtained with the use of a methanol: chloroform precipitation.

Understanding Actinides through the Role of 5f Electrons

MRS Bulletin ◽  
2001 ◽  
Vol 26 (9) ◽  
pp. 684-688 ◽  
Author(s):  
T. Gouder ◽  
F. Wastin ◽  
J. Rebizant ◽  
G.H. Lander
Keyword(s):  
Predictive Power ◽  
Quality Data ◽  
Nuclear Fuels ◽  
High Quality ◽  
High Quality Data ◽  
Long Term Storage ◽  
5F Electrons ◽  
Term Storage

Studies of the actinide elements and compounds were (and are) motivated by the need to characterize their structural and thermodynamic properties for the development of nuclear fuels and the treatment of waste, whether it be for long-term storage or ideas involving transmutation in high-powered accelerators. For the most part, tables giving these data exist, although the data for transuranium compounds are rather sparse. A much more difficult task is to understand the data and develop theories that have predictive power in this part of the periodic table. In doing this, however, we are confronted with the extremely complicated electronic structure of the 5f shell and the great paucity of high-quality data on materials containing transuranium isotopes.

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