scholarly journals Reevaluation of Salivary Lymphadenoma

2020 ◽  
Author(s):  
Chunye Zhang ◽  
Ting Gu ◽  
Yuhua Hu ◽  
Jingjing Sun ◽  
Ronghui Xia ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Epithelial Cells ◽  
Mucoepidermoid Carcinoma ◽  
Clinicopathologic Features ◽  
Prognostic Features ◽  
Lymphoid Stroma ◽  
Histologic Evaluation ◽  
Age Range

Context.— Both salivary lymphadenomas (LADs) and Warthin-like mucoepidermoid carcinoma (MEC) contain components of epithelium and lymphoid stroma and their differential diagnosis can be difficult on the basis of morphology alone. Objective.— To clarify whether Warthin-like MEC was diagnosed as a LAD, and to compare their clinicopathologic features. Design.— A total of 16 LAD cases were analyzed for MAML2 rearrangement by using fluorescence in situ hybridization, and the clinical, histologic, immunohistochemical, and prognostic features were compared between MAML2 rearrangement–positive and MAML2 rearrangement–negative groups. Results.— Among the 16 cases investigated, 9 harbored a MAML2 rearrangement and were reclassified as Warthin-like MEC. The remaining 7 cases were classified as LADs with 1 nonsebaceous and 6 sebaceous cases. The patients with Warthin-like MEC had a wider age range (10–75 years) than the patients with LADs (36–68 years). Histologically, 2 of the 9 Warthin-like MECs (22.2%) showed focal invasion, whereas all the LADs had complete capsules. Warthin-like MECs exhibited a diverse epithelial cell morphology, including basaloid, glandular, cuboidal, epidermoid, with mucinous cells, although these cytologic features were seen only focally in some cases. Nonsebaceous LAD was composed of basaloid and glandular epithelial cells predominantly. In sebaceous LAD, the epithelial cells were composed of basaloid and large foamy sebaceous cells. In all cases, the stroma was composed mainly of lymphocytes accompanied by lymphoid follicles, although plasma cell infiltration could be much heavier in Warthin-like MEC. All the patients had a good outcome after a longer follow-up (3∼166 months). Conclusions.— Warthin-like MEC can be misdiagnosed as a LAD owing to overlap in clinicopathologic features of the 2 entities. Careful histologic evaluation and detection of MAML2 rearrangement can facilitate their differential diagnosis.

Spermatocytic Tumor With Sarcoma: A Rare Testicular Neoplasm

2017 ◽  
Vol 25 (6) ◽  
pp. 559-562 ◽  
Author(s):  
Ashley E. Stueck ◽  
John E. Grantmyre ◽  
Lori A. Wood ◽  
Cheng Wang ◽  
Jennifer Merrimen
Keyword(s):  
Differential Diagnosis ◽  
Germ Cell ◽  
Poor Prognosis ◽  
Germ Cell Tumors ◽  
Testicular Neoplasm ◽  
Spermatocytic Seminoma ◽  
Isochromosome 12P ◽  
Pathologic Findings

Spermatocytic tumor, formerly known as spermatocytic seminoma, is an uncommon testicular neoplasm which is a distinct clinicopathologic entity from classic seminoma. These tumors are not associated with germ cell neoplasia in situ, other germ cell tumors, or isochromosome 12p. Although typically, these tumors have an excellent prognosis occasional cases are associated with sarcoma and have a very poor prognosis. We present a case of spermatocytic tumor with sarcoma showing a chondrosarcomatous component, discuss the pathologic findings and differential diagnosis and provide follow-up information.

scholarly journals Complex Intratarsal Cyst with a Mixed Ciliated Respiratory-Type and Squamous Epithelial Lining

2019 ◽  
Vol 6 (3) ◽  
pp. 151-158
Author(s):  
Frederick A. Jakobiec ◽  
Paula Cortes Barrantes ◽  
Lina Ma ◽  
Nahyoung Grace Lee
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Staining Pattern ◽  
Epithelial Lining ◽  
Full Thickness ◽  
Upper Eyelid ◽  
Eyelid Skin ◽  
Eyelid Swelling

A 55-year-old woman developed a painless, non-ulcerated left upper eyelid swelling over 6 months. Examination disclosed a fluctuant mass that permitted movement of the eyelid skin over the lesion. A full-thickness eyelid resection contained a well-encapsulated cyst with milky contents that was predominantly located in the tarsus. The cyst’s lining was partially composed of segments of ciliated respiratory-type and non-keratinizing squamous epithelia. Immunohistochemical evaluation with cytokeratins 17, 18, and 19 confirmed the staining pattern of a respiratory-type epithelial cell (whether or not cilia were present in the non-squamous epithelial zones). In the squamous region, entirely different cytokeratin results were obtained vis-a-vis the non-squamous regions of the lining. The current lesion is interpreted as congenital and representing an in situ persistence of embryonic ciliated glandular epithelium that normally exists only transitorily. A more remote possibility is that the lesion was the result of ectopic epithelial cells displaced from an adjacent sinus. A recurrence has not developed during 6 months of follow-up.

Clinical case of idiopathic pulmonary hypertension complicated by thrombosis in situ (9-year follow-up)

2021 ◽  
Vol 22 (2) ◽  
pp. 93-97
Author(s):  
D. F. Nizamova ◽  
◽  
Z. M. Safiullina ◽  
O. V. Abaturova ◽  
O. V. Kremneva ◽  
...  
Keyword(s):  
Pulmonary Hypertension ◽  
Differential Diagnosis ◽  
Clinical Case ◽  
Disease Development ◽  
Pathogenetic Mechanisms ◽  
Idiopathic Pulmonary Hypertension

The article presents a clinical case of idiopathic pulmonary hypertension complicated by thrombosis in situ (9-year follow-up). Possible pathogenetic mechanisms of the disease development are analyzed. The difficulties of differential diagnosis and the treatment of idiopathic pulmonary hypertension are discussed.

Primary cutaneous Ewing sarcoma/primitive neuroectodermal tumour: a clinicopathological analysis of seven cases highlighting diagnostic pitfalls and the role of FISH testing in diagnosis

2009 ◽  
Vol 62 (10) ◽  
pp. 915-919 ◽  
Author(s):  
M V Shingde ◽  
M Buckland ◽  
K J Busam ◽  
S W McCarthy ◽  
J Wilmott ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
In Situ Hybridisation ◽  
Molecular Testing ◽  
Fish Analysis ◽  
Fluorescence In Situ Hybridisation ◽  
Clinicopathological Analysis ◽  
Blue Cell

Aims:To perform a clinicopathological analysis of a series of primary cutaneous Ewing sarcomas/primitive neuroectodermal tumours (ES/PNET) to highlight the pathological features, discuss the differential diagnosis, emphasise the role of molecular testing (particularly fluorescence in situ hybridisation, FISH) in diagnosis and outline the patients’ clinical course.Methods:Seven cases of primary cutaneous ES/PNET were identified from the authors’ consultation files.Results:The patients were aged 16–61 years (median 25). Five were female and two were male. Five cases involved the limbs and two the trunk. Five were initially misdiagnosed (three as carcinoma and two as melanoma). All cases were characterised histologically by sheet-like growth of small round cells with little cytoplasm and showed strong membranous staining for CD99 and positive but variable staining for FLI-1. Six patients showed an EWS rearrangement (five on FISH analysis and one on RT-PCR). All tumours were completely excised. Three patients received adjuvant chemotherapy, one of whom also received radiotherapy. Follow-up was available in all cases (range 11–57 months; median 41). No recurrences or metastases occurred.Conclusions:Although rare, primary cutaneous ES/PNET should be considered in the differential diagnosis of cutaneous “small blue cell tumours”. Immunostaining for FLI-1 and molecular testing for evidence of an EWS rearrangement are useful ancillary investigations to confirm the diagnosis. The prognosis of primary cutaneous ES/PNET appears to be more favourable than extracutaneous ES/PNET.

scholarly journals BRAF Rearrangements and BRAF V600E Mutations Are Seen in a Subset of Pancreatic Carcinomas With Acinar Differentiation

2021 ◽  
Author(s):  
Toshi Ghosh ◽  
Patricia T. Greipp ◽  
Darlene Knutson; ◽  
Sara Kloft-Nelson; ◽  
Sarah Jenkins ◽  
...  
Keyword(s):  
Overall Survival ◽  
In Situ Hybridization ◽  
Mapk Pathway ◽  
Braf V600e Mutation ◽  
Braf V600e ◽  
Genomic Profiling ◽  
Clinicopathologic Features ◽  
Comprehensive Genomic Profiling

Context.— Comprehensive genomic profiling has demonstrated that approximately 20% of pancreatic carcinomas with acinar differentiation harbor potentially targetable BRAF fusions that activate the MAPK pathway. Objectives.— To validate the above finding by BRAF break-apart fluorescence in situ hybridization (FISH) in a large series of pure acinar cell carcinomas (ACCs), evaluate tumors for the presence of BRAF V600E mutations, and compare clinicopathologic features of tumors with BRAF rearrangements with those without. Design.— Thirty cases of pure ACC and 6 cases of mixed acinar-neuroendocrine carcinoma (ACC-NEC) were retrieved. A break-apart FISH probe was used to detect BRAF rearrangements. Immunohistochemistry for BRAF V600E was performed. Results.— BRAF rearrangements by FISH were found in 6 of 36 cases (17%), 5 of which were pure ACC and 1 was a mixed ACC-NEC. Follow-up was available in 29 of 36 (81%). The median survival was 22 months for BRAF-rearranged cases and 16 months for BRAF-intact cases; the 2-year overall survival was 50% for BRAF-rearranged cases and 35% for BRAF-intact cases. No significant clinicopathologic differences were identified in cases with BRAF rearrangement compared with those without BRAF rearrangement. BRAF V600E mutation was identified in 2 of 34 cases (6%), both of which were pure ACC and were BRAF-intact by FISH. Conclusions.— This study supports the finding that BRAF rearrangements are present in approximately 20% of cases and identified BRAF V600E mutations in approximately 5% of cases. These cases may benefit from targeted therapy.

scholarly journals Immunocytochemistry and DNA-Image Cytometry in Diagnostic Effusion Cytology I. Prevalence of Markers in Tumour Cell Positive and Negative Smears

1999 ◽  
Vol 19 (1) ◽  
pp. 7-20 ◽  
Author(s):  
Helma Motherby ◽  
Mary Kube ◽  
Nikolaus Friedrichs ◽  
Bahram Nadjari ◽  
Kristiane Knops ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Epithelial Cells ◽  
Tumour Cell ◽  
Image Cytometry ◽  
Metastatic Carcinoma ◽  
Carcinoma Cells ◽  
Malignant Cells ◽  
Cell Type ◽  
Dna Image Cytometry

To determine the prevalence of immunocytochemical positivities for a panel of antibodies in benign and malignant cells in effusions with known follow‐up in order to use these as diagnostic markers. Besides their ability to identify malignant epithelial cells their contribution to the differential diagnosis between carcinomatoses and mesotheliomas was investigated. 101 tumour cell positive and 53 negative effusions were stained with 12 different antibodies. Results were scored semiquantitatively per cell type. Furthermore, DNA‐image cytometry was performed. While prevalence of Ber‐EP4 positivity was 95.4% in metastatic carcinoma cells, it was 0% in those from mesotheliomas. No cell type reacted with this marker in benign effusions (0%). Ber‐EP4 correctly differentiated between metastatic carcinoma and mesothelioma in 98.0%. Prevalence of DNA‐aneuploidy was 95.4% in metastatic carcinomas, 57.1% in mesotheliomas and 0% in reactive effusions. Combining immunocytochemistry (Ber‐EP4 positivity) and DNA‐image cytometry (aneuploidy) results in a 100% detection of metastatic carcinomatoses and 57.1% of mesotheliomas. Both markers furthermore allowed a correct differentiation of these entities in 98%.

Mucoepidermoid Carcinoma of the Breast With MAML2 Rearrangement: A Case Report and Literature Review

2020 ◽  
Vol 28 (7) ◽  
pp. 787-792 ◽  
Author(s):  
Mingfei Yan ◽  
Hannah Gilmore ◽  
Aparna Harbhajanka
Keyword(s):  
Literature Review ◽  
Mucoepidermoid Carcinoma ◽  
Low Grade ◽  
Rare Entity ◽  
Carcinoma Of The Breast ◽  
Molecular Abnormalities ◽  
Molecular Abnormality ◽  
Hybridization Test

Mucoepidermoid carcinoma is one of the most common malignancies in salivary glands. In comparison, breast mucoepidermoid carcinoma is a very rare entity, with limited reports and understanding of its clinical behaviors to date. In this article, we report a case of low-grade breast mucoepidermoid carcinoma of a 60-year-old female patient. Histologic and immunohistochemical patterns were demonstrated. Fluorescence in situ hybridization test was also conducted to identify MAML2 rearrangement in this case, indicating a similar molecular abnormality as mucoepidermoid carcinoma in the salivary gland. Five-year follow-up of the patient showed no local recurrence or distant metastasis of the carcinoma, indicating the indolent behavior of low-grade breast mucoepidermoid carcinoma. Besides, a 40-year literature review from 1979 to 2019 was also performed to better characterize the prognosis and molecular abnormalities of the lesion.

scholarly journals Comparación entre los melanomas de cabeza y cuello con los de tronco y extremidades

2020 ◽  
Vol 112 (2) ◽  
pp. 157-164
Author(s):  
Silvina Verna ◽  
◽  
Gisela Coliva ◽  
Matías Norte ◽  
Fernando Dip ◽  
...  
Keyword(s):  
Head And Neck ◽  
Multidisciplinary Approach ◽  
Distant Metastases ◽  
Primary Lesion ◽  
Primary Defect ◽  
Melanoma In Situ ◽  
Clinical Records ◽  
Age Range

Background: Head and neck melanomas (HNMs) have been associated with prognostic factors different from those on other locations. Objective: The goal of the present study was to compare the demographic and clinical characteristics and the outcomes of surgical treatment between patients with HNM and those with trunk and extremity melanoma (TEM). Material and methods: The clinical records of patients undergoing surgery for melanoma between October 2014 and April 2018 were retrospectively reviewed. Fifteen patients (22.3%) had HNM and 52 (77.7) presented TEM. Results: There were no differences in age between both groups (63.8 ± 21.1 versus 58.5 ± 16), but there was a trend toward higher percentage of men in the HNM group (80% versus 61.3%). Patients with HNM had lower tumor thickness than those with TEM (2.07 versus 5.5 mm), higher incidence of melanoma in situ [5 (33.3%) versus 8 (15.3%)]; lymph node resection was more common (33% versus 25%) as well as reconstruction of the primary defect with local and musculocutaneous flaps. During follow-up, two patients in the HNM group developed local recurrences that were excised and three presented distant metastases in the lung, small bowel and abdomen and finally died due to the disease. In the TEM group, one patient had local recurrence and five died due to systemic metastases. The sample size was not sufficient to assess statistically significant differences. Conclusion: Head and neck melanomas occur in a wide age range and stages and has some clinical differences with TEM. The defects produced after the excision of the primary lesion often require more complex procedures and should be managed with a multidisciplinary approach.

scholarly journals Follicular lymphoma in situ in intra-abdominal lymphadenectomies- a study of 5 cases: revisiting the entity

2015 ◽  
Vol 1 (1) ◽  
pp. 62
Author(s):  
Rinsey R Kurian ◽  
Thiagarajah Balamurugan ◽  
Bruno Ping ◽  
Louise Hendry ◽  
Stephen Whitaker ◽  
...  
Keyword(s):  
Follicular Lymphoma ◽  
Incidental Finding ◽  
B Cell Lymphoma ◽  
Clinical Settings ◽  
Follicular Hyperplasia ◽  
Lymphoid Malignancies ◽  
Clinically Significant ◽  
Age Range

<p><strong>Background</strong>: Follicular lymphoma <em>in situ</em> (FLIS) is characterized by the presence of germinal centers that strongly express BCL-2 protein and germinal center markers CD10 and BCL-6, although most of the remaining lymph node shows a pattern of follicular hyperplasia, in the absence of interfollicular infiltration. Here, we present five cases of FLIS and discuss their presentation and pathological identification in a wide variety of clinical settings. <strong>Materials and Methods</strong>:<strong> </strong>The present study includes five cases of FLIS diagnosed in the department of surgical pathology over a period of three years (2010 to 2013). The clinical data and the follow-up information were obtained from the medical records. <strong>Results</strong>: The present study included three male and two female patients with an age range of 46–72 years. One case of FLIS was associated with diffuse large B-cell lymphoma (DLBCL), while in two cases this was an incidental finding associated with other non-lymphoid malignancies. <strong>Conclusion</strong>: FLIS has a very low rate of progression to clinically significant follicular lymphoma (FL), and the management strategy recommended is to watch and wait. However, some cases may develop into full blown FL and also many non-FL lymphoid malignancies have been seen associated with it. Hence, a staging workup is strongly advocated by many authors for FLIS.</p>

scholarly journals Soft tissue perineurioma involving the kidney: a report of two cases with an emphasis on differential diagnosis

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Tian-shi Ma ◽  
Ling Zhou ◽  
Quan Zhou ◽  
Xiang-lei He ◽  
Ming Zhao
Keyword(s):  
Differential Diagnosis ◽  
Soft Tissue ◽  
Low Grade ◽  
Renal Tubules ◽  
Case Presentation ◽  
Targeted Next Generation Sequencing ◽  
Mutation Burden ◽  
Fibromyxoid Sarcoma

Abstract Background Soft tissue perineurioma of the kidney is rare, with only a few reported cases. We report two additional cases with histologic, immunohistochemical and genetic analyses. Case presentation Both tumors were from adults (1 female aged 49 years and 1 male aged 42 years) and grossly had maximum diameters of 6.5 and 10 cm, respectively. The tumors were overall well circumscribed but unencapsulated, with focally entrapped benign native renal tubules in one case; both tumors seemed to arise in the capsular areas. The tumors had histologic and immunohistochemical profiles consistent with soft tissue perineurioma. Fluorescence in situ hybridization analyses demonstrated that the tumors were negative for amplification of MDM2 and rearrangements of ESWR1, FUS, and KMT2A. Targeted next-generation sequencing revealed a low tumor mutation burden and likely pathogenic mutations (CYP2B6 and FLT1 mutations for 1 each). Follow-up data were available for both patients; neither had tumor recurrence or metastasis. Conclusions In conclusion, renal perineurioma is rare, usually arises in the capsular areas, and is cured by resection. Low-grade dedifferentiated liposarcoma and low-grade fibromyxoid sarcoma as well as other spindle cell lesions should be considered in the differential diagnosis.

Sign in / Sign up

Export Citation Format

Share Document