Pulsed-field gel electrophoresis, plasmid profile and antimicrobial resistance pattern of Salmonella typhimurium isolated from human and retail meats

2012 ◽  
Vol 6 (22) ◽  
Author(s):  
Ines Abbassi-Ghozzi
Keyword(s):  
Antimicrobial Resistance ◽  
Salmonella Typhimurium ◽  
Gel Electrophoresis ◽  
Plasmid Profile ◽  
Pulsed Field Gel Electrophoresis ◽  
Resistance Pattern ◽  
Pulsed Field ◽  
Retail Meats

scholarly journals Molecular epidemiology and antimicrobial resistance of Salmonella isolates from broilers and pigs in Thailand

2019 ◽  
Vol 12 (8) ◽  
pp. 1311-1318 ◽  
Author(s):  
Dusadee Phongaran ◽  
Seri Khang-Air ◽  
Sunpetch Angkititrakul
Keyword(s):  
Antimicrobial Resistance ◽  
Gel Electrophoresis ◽  
Animal Species ◽  
Antimicrobial Agents ◽  
Genetic Relatedness ◽  
Pulsed Field Gel Electrophoresis ◽  
Resistance Pattern ◽  
Salmonella Spp ◽  
Pulsed Field ◽  
Amoxicillin Clavulanate

Aim: This study aimed to determine the prevalence and antimicrobial resistance pattern of Salmonella spp., and the genetic relatedness between isolates from broilers and pigs at slaughterhouses in Thailand. Materials and Methods: Fecal samples (604 broilers and 562 pigs) were collected from slaughterhouses from April to July 2018. Salmonella spp. were isolated and identified according to the ISO 6579:2002. Salmonella-positive isolates were identified using serotyping and challenged with nine antimicrobial agents: Amoxicillin/clavulanate (AMC, 30 μg), ampicillin (AMP, 10 μg), ceftazidime (30 μg), chloramphenicol (30 μg), ciprofloxacin (CIP, 5 μg), nalidixic acid (NAL, 30 μg), norfloxacin (10 μg), trimethoprim/sulfamethoxazole (SXT, 25 μg), and tetracycline (TET, 30 μg). Isolates of the predominant serovar Salmonella Typhimurium were examined for genetic relatedness using pulsed-field gel electrophoresis (PFGE). Results: Salmonella was detected in 18.05% of broiler isolates and 37.54% of pig isolates. The most common serovars were Kentucky, Give, and Typhimurium in broilers and Rissen, Typhimurium, and Weltevreden in pigs. Among broilers, isolates were most commonly resistant to antibiotics, NAL, AMP, TET, AMC, and CIP. Pig isolates most commonly exhibited antimicrobial resistance against AMP, TET, and SXT. Based on PFGE results among 52 S. Typhimurium isolates from broilers and pigs, a high genetic relatedness between broiler and pig isolates (85% similarity) in Cluster A and C from PFGE result was identified. Conclusion: The results revealed high cross-contamination between these two animal species across various provinces in Thailand. Keywords: antimicrobial resistance, broilers, pigs, pulsed-field gel electrophoresis, Salmonella spp.

Characterization of Antimicrobial-Resistant Phenotypes and Genotypes among Salmonella enterica Recovered from Pigs on Farms, from Transport Trucks, and from Pigs after Slaughter

2004 ◽  
Vol 67 (4) ◽  
pp. 698-705 ◽  
Author(s):  
WONDWOSSEN A. GEBREYES ◽  
PETER R. DAVIES ◽  
PAA-KOBINA TURKSON ◽  
W. E. MORGAN MORROW ◽  
JULIE A. FUNK ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Salmonella Typhimurium ◽  
Clavulanic Acid ◽  
Gel Electrophoresis ◽  
Rank Correlation ◽  
Pulsed Field Gel Electrophoresis ◽  
Mesenteric Lymph Nodes ◽  
Chloramphenicol Resistance ◽  
Pulsed Field ◽  
Amoxicillin Clavulanic Acid

The main objectives of this study were to determine antimicrobial resistance patterns among Salmonella serotypes and to evaluate the role of transport trucks in dissemination of antimicrobial-resistant strains of Salmonella. Salmonella from groups of nursery and finishing pigs on farms, from trucks, and from pigs after slaughter were compared using serotyping, patterns of antimicrobial resistance, and pulsed-field gel electrophoresis patterns. The five farms included in the study yielded 858 isolates representing 27 Salmonella serovars. The most common resistance observed (80% of all isolates) was to tetra-cycline; resistance to ampicillin (42%), chloramphenicol (31%), amoxicillin/clavulanic acid (30%), and piperacillin (31%) also were common. We found a correlation between serovar and antimicrobial resistance. High correlation was found between Salmonella Typhimurium var. Copenhagen and chloramphenicol resistance (Spearman rank correlation, ρ = 0.7). Multidrug resistance was observed primarily in Salmonella Typhimurium var. Copenhagen (94%) and Salmonella Typhimurium (93%) and was much less common in the other common serovars, including Salmonella Derby (7%) and Salmonella Heidelberg (8%). Of the 225 isolates exhibiting the most common pentaresistance pattern in this study, amoxicillin/clavulanic acid–ampicillin–chloramphenicol–piperacillin–tetracycline, 220 (98%) were Salmonella Typhimurium var. Copenhagen, and 86% of the isolates of this serovar had this pattern. Isolates from the trucks were similar, based on pulsed-field gel electrophoresis patterns, to those from the cecum and mesenteric lymph nodes of pigs on two of the farms, suggesting the probable infection of pigs during transport. Class I integrons were also common among various serovars.

Characterization and Antimicrobial Resistance of Salmonella Typhimurium Isolates from Clinically Diseased Pigs in Korea

2016 ◽  
Vol 79 (11) ◽  
pp. 1884-1890 ◽  
Author(s):  
SANG-IK OH ◽  
JONG WAN KIM ◽  
MYEONGJU CHAE ◽  
JI-A JUNG ◽  
BYUNGJAE SO ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Salmonella Typhimurium ◽  
Resistance Genes ◽  
Gel Electrophoresis ◽  
Salmonella Enteritidis ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Antimicrobial Resistance Genes

ABSTRACT This study investigated the prevalence of Salmonella enterica serovar and antimicrobial resistance in Salmonella Typhimurium isolates from clinically diseased pigs collected from 2008 to 2014 in Korea. Isolates were also characterized according to the presence of antimicrobial resistance genes and pulsed-field gel electrophoresis patterns. Among 94 Salmonella isolates, 81 (86.2%) were identified as being of the Salmonella Typhimurium serotype, followed by Salmonella Derby (6 of 94, 6.4%), Salmonella 4,[5],12:i:− (4 of 94, 4.3%), Salmonella Enteritidis (2 of 94, 2.1%), and Salmonella Brandenburg (1 of 94, 1.1%). The majority of Salmonella Typhimurium isolates were resistant to tetracycline (92.6%), followed by streptomycin (88.9%) and ampicillin (80.2%). Overall, 96.3% of Salmonella Typhimurium isolates showed multidrug-resistant phenotypes and commonly harbored the resistance genes blaTEM (64.9%), flo (32.8%), aadA (55.3%), strA (58.5%), strB (58.5%), sulII (53.2%), and tetA (61.7%). The pulsed-field gel electrophoresis analysis of 45 Salmonella Typhimurium isolates from individual farms revealed 27 distinct patterns that formed one major and two minor clusters in the dendrogram analysis, suggesting that most of the isolates (91.1%) from diseased pigs were genetically related. These findings can assist veterinarians in the selection of appropriate antimicrobial agents to combat Salmonella Typhimurium infections in pigs. Furthermore, they highlight the importance of continuous surveillance of antimicrobial resistance and genetic status in Salmonella Typhimurium for the detection of emerging resistance trends.

Antimicrobial resistance and genotypes of staphylococci from bovine milk and the cowshed environment

2012 ◽  
Vol 15 (4) ◽  
pp. 741-749 ◽  
Author(s):  
B. Kot ◽  
M. Piechota ◽  
M. Antos-Bielska ◽  
E. Zdunek ◽  
K.M. Wolska ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Gel Electrophoresis ◽  
Bovine Milk ◽  
Pulsed Field Gel Electrophoresis ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Haemolytic Activity ◽  
Pulsed Field ◽  
Sensitivity Pattern ◽  
Isolated Species

Abstract Investigation of antimicrobial resistance and genetic relatedness of staphylococci from milk of cows with mastitis and cowshed environment was the aim of this study. Antimicrobial resistance against 14 antimicrobials were determined by using a disc diffusion method. Genetic similarity between the most frequently isolated species was analysed by PFGE (pulsed-field gel electrophoresis). Haemolytic activity, DNase, protease and esterase production was also investigated. Coagulase-negative Staphylococcus species were isolated from 30.8% of milk samples from cows with mastitis. The most frequently isolated species was Staphylococcus xylosus and yield of these organisms was significantly associated with milk of mastitis cows. S. epidermidis was a predominant penicillin-resistant species. High frequency of resistance to lincomycin was observed among isolates of S. sciuri (54.2%) and S. xylosus (25.9%) from cows with mastitis. PFGE (pulsed-field gel electrophoresis) analysis of 29 Staphylococcus aureus isolates showed the presence of 17 PFGE pulsotypes. Isolates of S. sciuri (n=36) had unique PFGE patterns. Some S. xylosus isolates from milk and milker’s hands had the same PFGE pulsotypes, and this observation could indicate that dairyman may be a potential source of the infection. The pulsotype of each of the remaining isolates of S. xylosus suggested that they might have come from common environmental sources; however, these isolates differed in antibiotic resistance pattern or virulence traits. Therefore, knowledge about antibiotic sensitivity pattern and virulence factors of a CNS isolate, besides its genotype, may be informative in tracking the source of the infection.

scholarly journals Molecular Analyses of Salmonellaenterica Isolates from Fish Feed Factories and Fish Feed Ingredients

2003 ◽  
Vol 69 (2) ◽  
pp. 1075-1081 ◽  
Author(s):  
Live L. Nesse ◽  
Kerstin Nordby ◽  
Even Heir ◽  
Bjarne Bergsjoe ◽  
Traute Vardund ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Profile Analysis ◽  
Plasmid Profile ◽  
Pulsed Field Gel Electrophoresis ◽  
Fish Feed ◽  
Molecular Analyses ◽  
Feed Ingredients ◽  
Pulsed Field ◽  
Salmonella Serovars

ABSTRACT Isolates of the most commonly observed salmonella serovars in Norwegian fish feed factories from 1998 to 2000 (Salmonella enterica serovar Agona, S. enterica serovar Montevideo, S. enterica serovar Senftenberg, and S. enterica serovar Kentucky) were studied by pulsed-field gel electrophoresis (PFGE) and plasmid profile analysis and compared to isolates of the same serovars from fish feed ingredients, humans, and other sources (a total of 112 isolates). Within each serovar, a variety of distinct PFGE types (with similarity levels less than 90%) were observed in the feed ingredients and other sources, while only two distinct types of each serovar were identified in the factories. The combined results of PFGE and plasmid analyses showed that each factory harbored only a few S. enterica clones. Some of these clones persisted for at least 3 years in the factories, indicating that there was long-lasting contamination probably due to inadequate decontamination procedures.

scholarly journals Genotypic Characterization of Salmonella enteritidis Phage Types by Plasmid Analysis, Ribotyping, and Pulsed-Field Gel Electrophoresis

1998 ◽  
Vol 36 (8) ◽  
pp. 2314-2321 ◽  
Author(s):  
A. M. Ridley ◽  
E. J. Threlfall ◽  
B. Rowe
Keyword(s):  
Gel Electrophoresis ◽  
Salmonella Enteritidis ◽  
Profile Analysis ◽  
Phage Type ◽  
Plasmid Profile ◽  
Pulsed Field Gel Electrophoresis ◽  
Single Type ◽  
Pulsed Field ◽  
Phage Types ◽  
Reference Strains

Pulsed-field gel electrophoresis (PFGE) was used to resolveXbaI and SpeI macrorestriction fragments from 60 defined phage type (PT) reference strains of Salmonella enteritidis. The level of discrimination was compared to that afforded by plasmid profile analysis and ribotyping. Twenty-eight distinct XbaI pulsed-field profiles (PFPs) were observed, although a single type, PFP X1, predominated. Absence of the 57-kbspv-associated fragment was observed for three PT reference strains, and the profile was designated PFP X1A. The XbaI macrorestriction profiles of a further four PT reference strains were altered by the presence of plasmid-associated bands. Twenty-sixSpeI-generated PFPs (plus one subtype) were observed for the same strains. No SpeI fragment corresponding to the 38-MDa serovar-specific plasmid was detected. The distribution ofXbaI and SpeI profiles did not always correspond, producing a total of 32 combined PFPs for the 60 PT reference strains. This compared with a total of 18 different plasmid profiles and three PvuII ribotypes generated by the same strains. The results of this study indicate that PFGE may offer an improved level of discrimination over other genotypic typing methods for the epidemiological typing of S. enteritidis.

Isolation of Salmonella Typhimurium from Outbreak-Associated Cake Mix

2007 ◽  
Vol 70 (4) ◽  
pp. 997-1001 ◽  
Author(s):  
GUODONG ZHANG ◽  
LI MA ◽  
NEHAL PATEL ◽  
BALA SWAMINATHAN ◽  
STEPHANIE WEDEL ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Gel Electrophoresis ◽  
Ice Cream ◽  
Pulsed Field Gel Electrophoresis ◽  
Outbreak Investigation ◽  
Cake Batter ◽  
Pulsed Field ◽  
Enrichment Cultures ◽  
Broth Enrichment ◽  
Better Than

During May and June of 2005, 26 persons in several states were infected by a single strain (isolates indistinguishable by pulsed-field gel electrophoresis) of Salmonella enterica serotype Typhimurium after eating cake batter ice cream. The cake mix used to prepare the cake batter in the ice cream was implicated by epidemiologic investigation as the source of Salmonella contamination. Initial tests did not detect Salmonella in cake mix collected during the outbreak investigation. The objective of this study was to evaluate different procedures to isolate Salmonella from the implicated cake mix, cake, and ice cream. All outbreak-associated food samples (14 samples) were collected during the outbreak investigation by health departments of several of the states involved. Different combinations of Salmonella isolation procedures, including sample size, preenrichment broth, enrichment broth, enrichment temperature, and isolation medium, were used. Salmonella Typhimurium was isolated from two cake mix samples; the food isolates were indistinguishable from the outbreak pattern by pulsed-field gel electrophoresis subtyping. Universal preenrichment broth was substantially better than was lactose broth for preenrichment, and tetrathionate broth was better than was Rappaport-Vassiliadis broth for isolating Salmonella from the two positive cake mix samples. Although more typical Salmonella colonies were observed on plates from enrichment cultures grown at 35°C, more confirmed Salmonella isolates were obtained from plates of enrichment cultures grown at 42°C. Brilliant green agar, xylose lysine tergitol 4 agar, xylose lysine desoxycholate agar, Hektoen enteric agar, and bismuth sulfite agar plates were equally effective in isolating Salmonella from cake mix. The best combination of preenrichment-enrichment conditions for isolating the outbreak strain of Salmonella was preenrichment of cake mix samples in universal preenrichment broth at 35oC for 24 h, followed by enrichment in tetrathionate broth at 42°C for 24 h.

Sign in / Sign up

Export Citation Format

Share Document